Araceli A Cano-Estrada,
Miriam M Vázquez-Acevedo,
Alexa A Villavicencio-Queijeiro,
Francisco F Figueroa-Martínez,
Héctor H Miranda-Astudillo,
Yraima Y Cordeiro,
Julio A JA Mignaco,
Debora D Foguel,
Pierre P Cardol,
Marie M Lapaille,
Claire C Remacle,
Stephan S Wilkens and
Diego D González-Halphen
Abstract
We readdressed the overall topology of the enzyme with different experimental approaches: detection of close vicinities between subunits based on cross-linking experiments and dissociation of the enzyme into subcomplexes, inference of subunit stoichiometry based on cysteine residue labelling, and ge...
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PMID: 20188694
PDF is available here.
Abstract
We observed attraction toward microscale pulses of DMSP and related compounds among several motile strains of phytoplankton, heterotrophic bacteria, and bacterivore and herbivore microzooplankton. Because microbial DMSP cycling is the main natural source of cloud-forming sulfur aerosols, our results...
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PMID: 20647471
PDF is available here.
Abstract
Our results confirm that intense aerobic bacterial degradative processes can introduce a bias in palaeotemperature reconstructions especially when there is evidence of substantial aerobic bacterial degradation of the deposited organic matter. The widespread occurrence of epoxyalkenones in the marine...
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PMID: 20491919
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Abstract
I discuss eukaryotic deep phylogeny and reclassify the basal eukaryotic kingdom Protozoa and derived kingdom Chromista in the light of multigene trees. I transfer the formerly protozoan Heliozoa and infrakingdoms Alveolata and Rhizaria into Chromista, which is sister to kingdom Plantae and arguably...
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PMID: 20031978
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Abstract
We show here that specific stimulation of local CaMKIIalpha-expressing excitatory neurons, either in the neocortex or thalamus, elicits positive BOLD signals at the stimulus location with classical kinetics. We also show that optogenetic fMRI (of MRI) allows visualization of the causal effects of sp...
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PMID: 20473285
PDF is available here.
Abstract
We determined the toxicity of sulphamethoxazole, trimethoprim, flumequine, tylosin, streptomycin, and oxytetracycline, using the NAT adapted for water exposure. The sensitivity of the NAT was compared to that of bioassays with bacteria (Microtox), cyanobacteria and green algae. In the Microtox test...
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PMID: 20430416
PDF is available here.
Abstract
We here describe the metabolic engineering of an artificial pathway that produces 26% EPA in leaf triacylglycerol using a newly-identified Delta6-desaturase from the marine microalga Micromonas pusilla. We also demonstrate that this enzyme appears to function as an acyl-CoA desaturase that has prefe...
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PMID: 20004733
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Abstract
We did not find close relationship between heavy metal content (in localities as well as lichen thalli) and photobiont diversity. Presence of multiple algal genotypes in single lichen thallus has been confirmed.
Copyright 2009 Elsevier Inc. All rights reserved....
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PMID: 20031214
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Abstract
Biological tests are essential for toxicity assessment of chemicals to aquatic organisms. Since awareness is increasing that freely dissolved concentrations in test media may change during exposure and deviate significantly from nominal concentrations there is a need for concentration monitoring in...
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PMID: 20385402
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Abstract
We observed in both cases the ultrafast photoreduction of FAD(ox): in 390 fs for OtCPF1 and 590 fs for OtCPF2. Although such ultrafast electron transfer has already been reported for other flavoproteins and CPF members, the present result is the first demonstration with full spectral characterizatio...
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PMID: 20222748
PDF is available here.
Abstract
Mechanical fractionation and aqueous or aqueous/organic two-phase partition approaches were applied for extraction and separation of extracellular terpenoid hydrocarbons from Botryococcus braunii var. Showa. A direct spectrophotometric method was devised for the quantitation of botryococcene and ass...
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PMID: 20005092
PDF is available here.
Abstract
This paper aims to compare household waste, separated pig solids, food waste, pig slaughterhouse sludge and green algae regarding their biodegradability, their stabilization kinetics and their temperature rise during composting. Three experiments in lab-scale pilots (300 L) were performed for each w...
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PMID: 19954959
PDF is available here.
Abstract
We reported the nucleotide sequence of a 14-3-3 cDNA cloned from the unicellular green alga Dunaliella salina, however, the nucleotide sequence of this gene have not been reported so far. In the present study, the cloning and characterization of the nucleotide sequence, the gene copy and expression...
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PMID: 19806467
PDF is available here.
Abstract
It has been demonstrated that the duplicated carbonic anhydrase is induced by salt in the Dunaliella salina (D. salina) and duplicated carbonic anhydrase 1 (DCA1) is a member of carbonic anhydrase family. The purpose of this study was to identify whether both the DCA1 gene and its promoter from D. s...
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PMID: 19823944
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Abstract
We used strategy of shotgun proteomics to identify flagella proteins after flagella were released and collected from D. salina. A total of 520 groups of proteins were identified under a stringent filter condition (Xcorr > or =1.9, > or =2.2 and > or =3.75; DeltaCn >/= 0.1). In addition to six kinds...
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PMID: 19437132
PDF is available here.
Abstract
Although glucose-6-phosphate isomerase (GPI) plays an important role in glycolysis of both the prokaryotes and eukaryotes, studies on the GPI have not been involved in the halotolerant, unicellular green alga Dunaliella salina (D. salina). In this study, a 2,338 bp of full-length cDNA cloned using r...
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PMID: 19688275
PDF is available here.
Abstract
The circular mapping 49343 bp mitochondrial genome of Helicosporidium closely resembles that of the vertebrate parasite Prototheca wickerhamii. The two genomes share an almost identical gene complement and display a level of synteny that is higher than any other sequenced chlorophyte mitochondrial D...
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PMID: 20126458
PDF is available here.
Abstract
We consider two cultures: U. consortiale 960 and P. vulpinum 3957 to be the most promising for further researches, they differ by a wide spectrum of antibiotic and phytotoxic activity and were not studied before. The paper is presented in Ukrainian....
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PMID: 20455437
PDF is available here.
Abstract
Crassostrea virginica is an epibentic filter-feeding bivalve of economical importance in coastal lagoons of the Gulf of Mexico, locations with increasing inputs of heavy metals such as cadmium that have become environmental stressors. In this study, feeding and assimilation of the species were evalu...
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PMID: 20390836
PDF is available here.
Abstract
Xochimilco is an area of Mexico City fulfilling important ecological functions. However, the water of the canal network in the lacustrine zone of Xochimilco is supplied by the water treatment plants of the city, implying a risk of accumulated contaminants in the sediments. This study reports the eff...
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PMID: 20390837
PDF is available here.
Abstract
Phylogenetic evidence suggests GSIIB in Chloroplastida evolved by HGT, possibly after the divergence of the primary endosymbiotic lineages. Thus while multiple GS isoenzymes are common among members of the Chloroplastida, the isoenzymes may have evolved via different evolutionary processes. The acqu...
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PMID: 20579371
PDF is available here.
Abstract
We used laboratory cultures of clonal isolates of these two species to assay for the presence of viruses in seawater samples from diverse locations. In keeping with the distributions of their host strains, we found a decline in the abundance of O. tauri viruses from a lagoon in southwest France rela...
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PMID: 19897754
PDF is available here.
Abstract
Cladophora in the Great Lakes grows rapidly during the warm summer months, detaches, and becomes free-floating mats as a result of environmental conditions, eventually becoming stranded on recreational beaches. Cladophora provides protection and nutrients, which allow enteric bacteria such as Escher...
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PMID: 20595755
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Abstract
Species delimitation in lichen-forming fungi has been based largely on morphological differences of fungal structures and the lichen thallus with an assumption that morphology reflects hereditary changes. One of the distinguishing features between Cladonia pyxidata and C. pocillum (Cladoniaceae, Asc...
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PMID: 20524586
PDF is available here.
Abstract
The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high...
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PMID: 19507070
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Abstract
Four quantitative structure-activity relationships were developed for toxicity of selected photosynthesis (PHS) inhibitors and acetolactate synthase (ALS) inhibitors to Chlorella Vulgaris and Raphidocelis subcapitata using a mechanism-based approach. These models have good fitness and predictive abi...
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PMID: 19582361
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Abstract
We propose the delayed fluorescence (DF) measurement from an alga 24 h after exposure. Eight chemicals (bifenox, bromoxynil, bensulfuronmethyl, diuron, diflufenican, thiobencarb, m-chlorophenylhydrazone and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone) were tested. The EC50 values from the 24 h D...
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PMID: 19629368
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Abstract
Effect-directed analysis (EDA) has been frequently and successfully used to identify key toxicants in sediment extracts. However, by disregarding bioavailability this approach may lead to a biased prioritisation of fractions and toxicants with respect to hazards and risks. To overcome this problem t...
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PMID: 19848144
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Abstract
We have developed a liquid/flow cell/chamber allowing infrared measurements of living biological specimens with high spatial resolution under a controlled aqueous environment. This flow chamber features sub-micrometer thick diamond windows exhibiting low spherical and chromatic aberrations. Diamond...
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PMID: 19843370
PDF is available here.
Abstract
A new steroidal saponin, chloragin (
1), was isolated and characterised from the aerial part of
Chlorophytum nimonii. The structure of chloragin (
1) was established as tigogenin-3-
O-α-
L-rhamnopyranosyl-(1 → 4)-β-
D
PDF is available here.
Abstract
These results indicate usefulness of HiCEP for screening of stress-responsive genes in species that are ecotoxicologically important but for which genomic sequence information is lacking....
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PMID: 19462152
PDF is available here.
Abstract
Bioassays were performed by commercially available kits on peracetic acid (PAA) solutions, at different concentrations, and on secondary effluents (from two different wastewater treatment plants) after disinfection at bench-scale, considering both samples containing residual active PAA and the same...
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PMID: 19764220
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Abstract
We demonstrate that this accumulation is due to the presence of an external electron donor agent in the purification buffer. Composition changes observed under steady-state photoexcitation are interpreted in terms of photoinduced reductions of FAD(ox) and FADH* states and competitive back reactions....
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PMID: 19427226
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Abstract
These results suggest that FG might be a potent candidate as a prophylactic agent for HSV-2 infection....
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PMID: 19420760
PDF is available here.
Abstract
The novel way of advanced treatment of municipal wastewater was proposed and characterized by purifying wastewater by Spirogyra. The characteristics of nitrogen and phosphorus removal by Spirogyra were studied aiming at nitrogen and phosphorus removal in advanced treatment of sewage. Under natural l...
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PMID: 19545007
PDF is available here.
Abstract
We describe the osmotic response process of Dunaliella under salinity stress, including the changes of cell volume, intracellular ions concentration, intracellular glycerol concentration, and the expression of some salt-induced genes. Some specific proteins and enzymes can be induced by different sa...
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PMID: 19202552
PDF is available here.
Abstract
We constructed inducible and constitutive heterotrophic expression vectors of Dunaliella salina (D. salina) and identified heterotrophic transformants. A gene encoding a glucose transporter (Glut1) was cloned from human placenta tissues by RT-PCR and sequenced. Inducible heterotrophic expression vec...
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PMID: 19621580
PDF is available here.
Abstract
We report the presence of group I introns at these positions within the chloroplast-encoded large subunit ribosomal RNA gene of several lichen-forming green algae belonging to the Trebouxia genus. In contrast to the introns inserted at position 2449, all introns inserted at positions 1917, 1931, and...
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PMID: 19440984
PDF is available here.
Abstract
Most flagellate green algae exhibiting phototaxis posses a singular specialized light sensitive organelle, the eyespot apparatus (EA). Its design principles are similar in all green algae and produce, in conjunction with the movement pattern of the cell, a highly directional optical device. It enabl...
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PMID: 19107486
PDF is available here.
Abstract
Biofuels demand is unquestionable in order to reduce gaseous emissions (fossil CO(2), nitrogen and sulfur oxides) and their purported greenhouse, climatic changes and global warming effects, to face the frequent oil supply crises, as a way to help non-fossil fuel producer countries to reduce energy...
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PMID: 18982369
PDF is available here.
Abstract
This study investigated the growth inhibition effects of three phenicol antibiotics on microalgae used in aquaculture. Different dose levels of chloramphenicol (CAP), florfenicol (FF), and thiamphenicol (TAP) were added to cultures of one freshwater green alga, Chlorella pyrenoidosa, and two marine...
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PMID: 18439675
PDF is available here.
Abstract
These results show the advantages of having a tiered approach within a test battery. The presented results indicate that sertraline hydrochloride adversely affects aquatic organisms at levels several orders of magnitude higher than that reported in municipal effluent concentrations, however adverse...
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PMID: 18571724
PDF is available here.
Abstract
The objective of this study was to investigate the effect of dissolved organic ligands, such as EDTA, humic acids, hydrophobic and hydrophilic fractions of dissolved organic matter (DOM), on the growth of Pseudokirchneriella subcapitata in cultured media and natural lake waters. The growth of algae...
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PMID: 18313752
PDF is available here.
Abstract
We present first evidence for putative regulatory nucleotide sequences which will be useful in future studies on nickel uptake and incorporation....
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PMID: 19253325
PDF is available here.
Abstract
We investigated the effect of NO on development in the unicellular green alga, Micrasterias denticulata, using two different NO donors, S-nitroso-N-acetyl-dl-penicillamine (SNAP) and sodium nitroprusside (SNP). Investigations at the light microsopic level revealed that both NO donors suppressed cell...
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PMID: 18455833
PDF is available here.
Abstract
Current anthropogenic activities have been causing a significant increase in the atmospheric concentration of CO2 over the past 60 years. To mitigate the consequent global warming problem, efficient technological solutions, based on economical and technical grounds, are required. In this work, micro...
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PMID: 18767189
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Abstract
The feasibility of a one-step method for the continuous production of astaxanthin by the microalga Haematococcus pluvialis has been verified outdoors. To this end, influence of dilution rate, nitrate concentration in the feed medium, and irradiance on the performance of continuous cultures of H. plu...
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PMID: 18767188
PDF is available here.
Abstract
Multidirectional effects of symbionts in biocenosis were revealed. Involvement of functional systems lysozyme-antilysozyme and hydrogen peroxide-catalase in regulation of associative interactions in algae-bacterial biocenosis was established. In conditions of antibacterial action of algae's hydrogen...
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PMID: 19715206
PDF is available here.
Abstract
We demonstrate the galvanotactic controls of the cellular migration of P bursaria in capillary tubes (diameter, 1-2 mm; length, 30-240 mm). Since the Paramecium cells take up particles of various sizes, we attempted to use the electrically stimulated cells of P bursaria as the vehicle for transporta...
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PMID: 19678550
PDF is available here.
Abstract
The green algaVolvoxrepresents the simplest multicellular organism:Volvoxis composed of only two cell types, somatic and reproductive.Volvox, therefore, is an attractive model system for studying various aspects of multicellularity. With the biolistic nuclear transformation ofVolvox carteri, the powe...
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PMID: 10069071
PDF is available here.
Abstract
Programmed cell death (PCD) is a key element in normal plant growth and development which may also be induced by various abiotic and biotic stress factors including salt stress. In the present study, morphological, biochemical, and physiological responses of the theoretically immortal unicellular fr...
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PMID: 19213813
PDF is available here.
Abstract
Within the Chlorophyta, EF-1alpha is shown to be present in three ulvophycean orders (i.e., Dasycladales, Bryopsidales, Siphonocladales) and the genus Ignatius. Models describing gene gain-loss dynamics revealed that the presence of EF-1alpha, EFL or both genes along the backbone of the green plant...
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PMID: 19216746
PDF is available here.
Abstract
We studied the early steps in the isoprenoid pathway for the biosynthesis of carotenoids and beta-carotene and the effects of metabolic inhibitors. When D. salina was grown under carotenogenic and non-carotenogenic conditions, mevinolin did not inhibit growth or the accumulation of carotenoids, beta...
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PMID: 19521917
PDF is available here.
Abstract
Stable nuclear transformation of G. pectorale was achieved using a heterologous dominant antibiotic resistance gene, the aminoglycoside 3'-phosphotransferase VIII gene (aphVIII) of Streptomyces rimosus, as a selectable marker. Heterologous 3'- and 5'-untranslated flanking sequences, including promot...
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PMID: 19591675
PDF is available here.
Abstract
A single-copy gene is found in the six chlorophyte green algae, which is most closely related to the CslA and CslC families that are present in the seven land plants investigated in our analyses. Six proteins from poplar, grape and sorghum form a distinct family (CslJ), providing further support for...
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PMID: 19646250
PDF is available here.
Abstract
The adverse effects of rainwater and artificial urban runoff (an imitation of urban runoff during rainfall event) were investigated using a battery of bioassays employing three test species. Urban dusts were collected at three representative sites; commercial, industrial and highway areas. The water...
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PMID: 19494463
PDF is available here.
Abstract
Fourteen blue-green and green algae survived for widely different time periods ranging between 22-102 d in control culture medium. Irrespective of their long or short survival period in control cultures, their pro- or eukaryotic nature, their different morphological types or natural habitats, they a...
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PMID: 19330547
PDF is available here.
Abstract
Addition of anionic polyacrylamide (PAM) to agricultural irrigation water can dramatically reduce erosion of soils. However, the toxicity of PAM to aquatic life, while often claimed to be low, has not been thoroughly evaluated. Five PAM formulations, including two oil-based products, one water-based...
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PMID: 19141814
PDF is available here.
Abstract
A homogeneous sulfated heterorhamnan was obtained by aqueous extraction, then by ultrafiltration from the green seaweed Gayralia oxysperma. Besides alpha-L-rhamnose it contains glucuronic and galacturonic acids, xylose and glucose. The structure was established by methylation analyses of the carboxy...
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PMID: 18845298
PDF is available here.