Abstract
I discuss eukaryotic deep phylogeny and reclassify the basal eukaryotic kingdom Protozoa and derived kingdom Chromista in the light of multigene trees. I transfer the formerly protozoan Heliozoa and infrakingdoms Alveolata and Rhizaria into Chromista, which is sister to kingdom Plantae and arguably...
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PMID: 20031978
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Abstract
We describe the complete plastid genome sequences and plastid-associated data from two independent photosynthetic lineages represented by Chromera velia and an undescribed alga CCMP3155 that we show are closely related to apicomplexans. These plastids contain a suite of features retained in either a...
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PMID: 20534454
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Abstract
We previously devised a novel synchronous culture system with controlled photosynthesis, which is triggered by 6 h-light/18 h-dark cycles combined with different levels of CO(2). The cells do not enter S-phase and consequently do not divide after the minimum light period without CO(2) supplementatio...
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PMID: 20223803
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Abstract
Hybrid iota-/nu-carrageenan was water-extracted from Eucheuma denticulatum and incubated with Alteromonas fortis iota-carrageenase. The degradation products were then separated by anion-exchange chromatography. The three most abundant fractions of hybrid iota-/nu-carrageenan oligosaccharides were pu...
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PMID: 20227066
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Abstract
A gene (agrP) encoding a beta-agarase from Pseudoalteromonas sp. AG4 was cloned and expressed in Escherichia coli. The agrP primary structure consists of an 870-bp open reading frame (ORF) encoding 290 amino acids (aa). The predicted molecular mass and isoelectric point were determined at 33 kDa and...
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PMID: 20213114
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Abstract
We hypothesized that the CMG021C gene product functions as an NiR in C. merolae. To test this hypothesis, we developed a method for targeted gene disruption in C. merolae. In support of our hypothesis, we found that a CMG021G null mutant in comparison with the parental strain showed decreased cell g...
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PMID: 20375110
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Chika Hirabaru,
Asako Izumo,
Shoko Fujiwara,
Yukie Tadokoro,
Takahiro Shimonaga,
Mai Konishi,
Mayumi Yoshida,
Naoko Fujita,
Yasunori Nakamura,
Masaki Yoshida,
Tsuneyoshi Kuroiwa and
Mikio Tsuzuki
Abstract
Our results support an evolutionary scenario defined by the loss of starch and reversion to glycogen synthesis during the evolution of cyanidian algae, and suggest the possibility that a C. merolae-like primitive rhodophyte might have evolved into the Florideophycidae....
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PMID: 20385610
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Abstract
We must understand not only mitotic division but also organelle division cycles. Plant and animal cells contain many organelles which divide randomly; therefore, it has been difficult to elucidate these organelle division cycles. We used the primitive red alga Cyanidioschyzon merolae, as it contains...
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PMID: 20148273
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Abstract
Cyanidium caldarium (Tilden) Geitler SAG 16.91 has been encapsulated within a porous silica host structure to target novel photosynthetic hybrid materials suitable for use in solar cells or CO(2) fixation. C. caldarium cells are both thermophilic and acidophilic; on account of these tolerances the h...
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PMID: 20138290
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Abstract
Shallow-water hydrothermal activity is widely present at Azores archipelago. Organisms in such environments present great potential as sentinels of the effects derived from chronically exposure to increased temperature, metal concentrations and reduced pH. This study aimed to evaluate metal concentr...
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PMID: 20042204
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Abstract
We show that C. merolae contains about four vacuoles that are distributed equally between the daughter cells by binding to dividing mitochondria. Binding is mediated by VIG1, a 30-kD coiled-coil protein identified by microarray analyses and immunological assays. VIG1 appears on the surface of free v...
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PMID: 20348431
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Abstract
Copper complexation in marine systems is mainly controlled by organic matter, partially produced by micro- and macroalgae that release exudates with the capacity to bind metals. This feature is important as it influences bioavailability, bioaccumulation, toxicity, and transport of copper through bio...
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PMID: 19962173
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Abstract
We produced a recombinant form of CmSiRB and characterized its enzymatic properties. The enzyme was found to have a significant nitrite-reducing activity, whereas its sulfite-reducing activity was extremely low. As the affinity of CmSiRB for sulfite was higher by 25-fold than that for nitrite, nitri...
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PMID: 19622064
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Abstract
Six 1-glyceryl ethers (1-6) were isolated from the red alga-sponge assemblage Ceratodictyon spongiosum/Haliclona cymaeformis. Structural assignments were conducted by interpretation of spectroscopic data and the modified Mosher's method. Four allylic alcohols were obtained as a pair of epimeric mixt...
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PMID: 19670881
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Abstract
We describe the carbohydrate-binding properties of the three isolectins (hypninA1, A2, and A3) and the amino acid sequence of hypninA3 (P85888). In frontal affinity chromatography with about 100 pyridylaminated oligosaccharides, the isolectins, which had no affinity for monosaccharides, commonly bou...
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PMID: 19352030
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Abstract
We review the recent applications of ancient HGT events in reconstructing organismal phylogeny as well as the promise and potential pitfalls of this approach....
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PMID: 19271182
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Abstract
An enzyme with sarcosine dimethylglycine methyltransferase (SDMT) activity has been identified in the thermophilic eukaryote, Galdieria sulphuraria. The crystal structure of the enzyme, solved to a resolution of 1.95 A, revealed a fold highly similar to that of mycolic acid synthases. The kcat and a...
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PMID: 18623062
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Abstract
The identification of the polysaccharides from tetrasporophytic plants of nine endemic New Zealand species belonging to the Gigartinaceae, 'Gigartina' ancistroclada, 'G.' grandifida, Gigartina dilatata, G. divaricata, G. macrocarpa, G. marginifera, G. pachymenioides, G. sp. 'Lindauer 164' and Sarcot...
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PMID: 19007924
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Abstract
We show that NDR is regulated by a tetrapyrrole signal in plant cells, which has been suggested as an organelle-to-nucleus retrograde signal. In synchronized cultures of the primitive red alga Cyanidioschyzon merolae, specific inhibition of A-type cyclin-dependent kinase (CDKA) prevented NDR but not...
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PMID: 19141634
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Abstract
Investigation of minor metabolites in the extracts of the red alga Sphaerococcus coronopifolius collected from the rocky coasts of Corfu Island in the Ionian Sea yielded two new diterpene alcohols, sphaerollanes I, and II (1, 2) featuring neodolabellane skeletons, and the new sphaeroane diterpene al...
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PMID: 19597580
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Abstract
OBJECTIVE: To study the chemical constituents of the red alga Acanthophora spicifera boergesen aiming at searching for bioactive leading compounds. METHOD: Compounds were isolated by various chromatographic techniques including column chromatography over normal phase silica gel and Sephadex LH-20 ge...
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PMID: 19382453
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Abstract
Contingency tests of specific predictions of the chromalveolate model find no evidence for an unusual red algal contribution to Phytophthora genomes, nor that putative cyanobacterial sequences that are present entered these genomes through a red algal endosymbiosis. Examination of genes unrelated to...
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PMID: 19843329
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Abstract
The lipid class and fatty acid composition of a little-known and rarely collected alga Exophyllum wentii from Bali Island, Indonesia were determined for fresh and frozen-thawed samples using thin-layer chromatography, gas-liquid chromatography, and high-performance liquid chromatography. Glycoglycer...
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PMID: 19202307
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Abstract
A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, an...
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PMID: 18978081
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Abstract
This study focused on the biosorption of total chromium onto red algae (Ceramium virgatum) biomass from aqueous solution. Experimental parameters affecting biosorption process such as pH, contact time, biomass dosage and temperature were studied. Langmuir, Freundlich and Dubinin-Radushkevich (D-R) m...
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PMID: 18406520
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Abstract
We report on the characterization of three P. yezoensis genes, a nitrate transporter (PyNRT2) and two urea transporters (PyUT1 and PyUT2), which may be involved in reversing iroochi. The predicted length of the PyNRT2 protein was 479 amino acids (AA), while PyUT1 and PyUT2 were 740 and 680 AA, respe...
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PMID: 18202829
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Abstract
Transmission electron microscopy revealed the presence of electron-dense bodies (EDB) in the cytosol of the acidophilic, thermophilic red alga Cyanidium caldarium. These bodies contain almost exclusively Fe, P, and O and can play a role in Fe storage. (31)P-nuclear magnetic resonance analysis identi...
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PMID: 18575816
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Abstract
Phytochelatin synthase (PCS) catalyzes the synthesis of phytochelatins (PCs), which play a detoxification role in higher plants. Heterologous expression of CmPCS, a product of a PCS-like gene from the genomic DNA of the red alga Cyanidioschyzon merolae, rescued Cd(2+)-sensitive yeast from Cd(2+) tox...
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PMID: 19060382
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Abstract
Lobophora variegata and Udotea conglutinata demonstrated promising anti-trichomonal potential and have been selected for further bio-guided fractionation and isolation of active anti-trichomonal compounds....
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PMID: 18725281
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Abstract
We describe the mechanics of genicula in the articulated coralline Calliarthron and demonstrate how segmentation affects bending performance and amplifies bending stresses within genicula. A numerical model successfully predicted deflections of articulated fronds by assuming genicula to be assemblag...
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PMID: 18931315
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Abstract
We explored constraints on the size of the articulated coralline alga Calliarthron, which thrives in wave-exposed intertidal habitats. Its ability to survive depends critically upon its segmented morphology (calcified segments separated by flexible joints or ;genicula'), which allows otherwise rigid...
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PMID: 18931316
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Abstract
We thus hypothesized that red algal transporters have distinct substrate specificity in comparison to their green orthologs. This hypothesis was tested by expression of the red algal genes in yeast (Saccharomyces cerevisiae) and assessment of their substrate specificities and kinetic constants. Inde...
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PMID: 18799657
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Abstract
The biosorption characteristics of uranium(VI) onto Catenella repens (a red alga), were evaluated as a function of pH, biosorbent size, time, biomass dosage, initial uranium concentration and temperature. Within the pH range studied (1.5-7.5), 4.5 was the optimum pH for the uptake of uranium(VI) by...
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PMID: 18375055
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Abstract
Based on the survey of benthic algae in the intertidal zone of Ma' an Archipelago from March to July 2007, the algal species composition, distribution, and temperature feature were studied. The dominant algal species in the study area were preliminarily analyzed by using similarity indices (S(c)) an...
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PMID: 19123370
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Abstract
OBJECTIVE: To study the protein tyrosine phosphatase-1B (PTP1B) inhibitory activity of natural products from algae aiming at searching for new way for the treatment of type 2 diabetes mellitus (T2DM) and obesity. METHOD: Bromophenols derivatives from algae were screened against the PTP1B by the colo...
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PMID: 19166016
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Abstract
Phytochemical analysis of Fijian populations of the green alga Tydemania expeditionis led to the isolation of two unsaturated fatty acids, 3(zeta)-hydroxy-octadeca-4(E),6(Z),15(Z)-trienoic acid (1) and 3(zeta)-hydroxy-hexadeca-4(E),6(Z)-dienoic acid (2), along with the known 3(zeta)-hydroxy-octadeca...
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PMID: 18757069
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Abstract
The interspecific competition between Ulva pertusa and Grateloupia filicina and it's response to the UV-B irradiation enhancement were analyzed using mono-culture and co-culture methods. The study adopted reasonable experimental design and took biomass as the main examined index. Results showed that...
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PMID: 19143368
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Abstract
The biosorption characteristics of Cd(II) ions using the red alga (Ceramium virgatum) were investigated. Experimental parameters affecting the biosorption process such as pH, contact time, biomass dosage and temperature were studied. Langmuir, Freundlich and Dubinin-Radushkevich (D-R) models were ap...
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PMID: 18280037
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Abstract
The aim was to evaluate the antioxidant defences and the occurrence of oxidative damage in Posidonia oceanica under a stress situation due to the epiphytism of the invasive macroalgae Lophocladia lallemandii. P. oceanica leaves were collected in the absence (control station) and in the presence of t...
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PMID: 18639925
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Abstract
Investigation of the secondary metabolites of the marine-derived fungus Curvularia sp. yielded four new 10-membered lactones (1-4), along with the known modiolide A (5). The structures of 1-4 were characterized on the basis of spectroscopic and MS data and resemble known 10-membered lactones, but fe...
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PMID: 18729518
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Abstract
We should not have been able to observe that the most frequent class of permuted genes is that of the anticodon loop type, for the simple reason that this class would derive by random permutation from a class of non-permuted tRNA genes, which instead is the rarest. This would not explain the high fr...
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PMID: 18513750
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Abstract
We also focus on the recently identified pathways for the synthesis of both molecules, which were elucidated by studying hyper/thermophilic MG-accumulating organisms. The blooming era of genomics has now allowed the detection of these genes in fungi and mosses, opening a research avenue that spans t...
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PMID: 18599240
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Abstract
We conclude that modern ulvophyte and early diverging prasinophyte green algae produce high abundances of C(29) relative to C(27) and C(28) sterols most consistent with the sterane profiles observed in Paleozoic rocks. Our analysis also suggests that ancestral stem groups among the Plantae, includin...
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PMID: 18624688
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Abstract
We have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 microg/ml were much lowe...
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PMID: 18572208
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Abstract
We identify highly inductive natural cues of the tropical abalone Haliotis asinina. In contrast to reports for other abalone, the greatest proportion of H. asinina larvae are induced to settle and metamorphose (92.8% to 100% metamorphosis by 48 h postinduction) by articulated corallines of the genus...
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PMID: 18723641
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Abstract
C-phycocyanin (C-PC) is a blue pigment in cyanobacteria, rhodophytes and cryptophytes with fluorescent and antioxidative properties. C-PC is presently extracted from open pond cultures of the cyanobacterium Arthrospira platensis although these cultures are not very productive and open for contaminat...
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PMID: 18563408
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Abstract
The taxonomic criterion of Antithamnion sparsum was reappraised in comparison with A. densum and A. defectum based on crossing experiments, morphological observation, chromosome study and Random Amplified Polymorphic DNA (RAPD) analysis. These species had a very similar morphology but were sexually...
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PMID: 19195395
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Abstract
These results suggested that this Chlamydomonas might be a facultative epiphyte benefiting from its hosts. Several ceramiaceaen algae were tested as host plants. Meanwhile, cell deformation or collapse of the whole thallus was caused to Aglaothamnion byssoides, and preliminary study suggested that a...
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PMID: 19195375
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Abstract
Two inhibitors of Taq DNA polymerase were isolated from the marine red alga Symphyocladia latiuscula. The inhibitors were purified by methanol extraction, molecular fractionation below 3000 MW and reverse-phase HPLC. The purified compound SL-1 containing three bromines was identified as 2,3,6-tribro...
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PMID: 19195384
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Abstract
This work reports the results of the study for cadmium binding by the dead red macroalga Mastocarpus stellatus. Kinetics sorption experiments demonstrated the high rate of metal biosorption: the system attained over 50% of the total biomass cadmium uptake within 2 min of contact and over 90% in the...
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PMID: 17928221
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Abstract
We examined the relationship between the protein content and the fermentation of nori by means of bfidobacteria. nori with a low protein content (25% on dry base) was strongly fermented by bifidobacteria, whereas nori with a high protein content (41% on dry base) was not. nori with a low protein con...
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PMID: 18603784
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Abstract
We report the molecular and biochemical characterization of algal GSTs. Phylogenetic analysis showed that most of them were distinct from previously described GST classes, but were most closely related to the Sigma class. Profiling of GST genes from the red alga Chondrus crispus and brown alga Lamin...
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PMID: 18315526
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Abstract
Biosorption of Pb(II), Cu(II), Cd(II) and Zn(II) from binary metal solutions onto the algae Gelidium sesquipedale, an algal industrial waste and a waste-based composite material was investigated at pH 5.3, in a batch system. Binary Pb(II)/Cu(II), Pb(II)/Cd(II) and Pb(II)/Zn(II) solutions have been t...
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PMID: 18055109
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Abstract
Continuous metal ions biosorption from Pb/Cu and Pb/Cd solutions onto seaweed Gelidium sesquipedale and a composite material prepared from an industrial algal waste was performed in a packed bed column. A binary Langmuir equation describes well the equilibrium data and indicates a good adsorption ca...
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PMID: 18162305
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Abstract
The type specimen of the red alga Alysium holtingii C. Agardh, described from Brazil, is located in the Lund Herbarium, and it is depicted for the first time in a publication. It is taxonomically identical to Dichotomaria obtusata (J. Ellis and Solander) Lamarck and thus can be treated as a later ta...
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PMID: 18506258
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Abstract
Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) and methoxylated polybrominated diphenyl ethers (MeO-PBDEs) are present in the ecosystem of the Baltic Sea. OH-PBDEs are known to be both natural products from marine environments and metabolites of the anthropogenic polybrominated diphenyl ethe...
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PMID: 18457860
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