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Aptamers, Peptide (0):
Recent article
Aptamers, Peptide
Analyst (Cambridge UK) (135)11 2010
Abstract
We herein describe a novel magnetic beads-based aptameric real-time PCR assay for the accurate quantification of rHuEPO-α, which combined the specific recognition with amplification capability of aptamers. Two detection strategies, termed 'recognition-after-hybridization' and 'recognition-before-hy...
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PMID: 20877820
PDF is available here.
PDF is available here.
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British Journal of Cancer (103)8 2010
Abstract
Inhibitors of DNA-binding proteins (Id1-4), lacking the basic DNA-binding domain, function as dominant inhibitors of cell-cycle regulators. Overexpression of Id proteins promotes cancer cell proliferation and resistance against apoptosis. Level of Id protein expression, especially of Id1, correlates...
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PMID: 20842131
PDF is available here.
PDF is available here.
Abstract
We want curative or near-curative therapy that will return patients to a normal state of health at a feasible cost, much better diagnostic and therapeutic technologies need to be developed. This review addresses the vexing group of heart failure etiologies that include cardiomyopathies and other ven...
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PMID: 20869655
PDF is available here.
PDF is available here.
Hereditas (Beijing) (32)6 2010
Abstract
We briefly review the major progress of peptide aptamer in validating gene function, and emphasize its promising application in both plant functional genomics and peptide-mediated broad-spectrum plant resistance to diverse phytoviruses. The strategies to overcome the potential risks are covered as w...
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PMID: 20566457
PDF is available here.
PDF is available here.
Abstract
Targeted delivery of therapeutics is an area of vigorous research, and peptide- and aptamer-functionalized nanovectors are a promising class of targeted delivery vehicles. Both peptide- and aptamer-targeting ligands can be readily designed to bind a target selectively with high affinity, and more im...
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PMID: 19655996
PDF is available here.
PDF is available here.
Abstract
Here, a fluorescent switch is constructed combining hemin, hemin aptamer, and a newly synthesized anionic conjugated polymer (ACP), poly(9,9-bis(6'-phosphatehexyl) fluorenealt-1,4-phenylene) sodium salt (PFHPNa/PFP). In the "off-state", the fluorescence of PFP is sensitively quenched by hemin, with...
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PMID: 19344125
PDF is available here.
PDF is available here.
Abstract
We describe, specifically, the optimization of an orientated peptide aptamer surface and its utilization in establishing a highly specific, low-nanomolar sensitive, detection protocol for the active form of CDK2. In comparing target binding affinity of two different aptamers (pep6 and pep9), both co...
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PMID: 19320493
PDF is available here.
PDF is available here.
Abstract
Peptide aptamers are specific binders that are artificially created using in vitro evolution systems. The peptide aptamers that can detect malignant cells in vivo, or can bind surfaces of various materials will enable us to develop novel types of cancer diagnosis and cancer therapy agents by combini...
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PMID: 19295259
PDF is available here.
PDF is available here.
Abstract
The set-up presented in this article is intended for the selection of peptides which serve as specific binders to suitable materials. Additionally, the interaction of such binders with material surfaces can be characterized. Using this approach, a subset of peptides which adhere to the mineral TiO(2...
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PMID: 18977188
PDF is available here.
PDF is available here.
Abstract
We present a high-throughput screening assay that identifies small molecules that displace interactions between proteins and their cognate peptide aptamers. AptaScreen is a duplex yeast two-hybrid assay featuring two luciferase reporter genes. It can be performed in 96- or 384-well plates and can be...
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PMID: 19377984
PDF is available here.
PDF is available here.
Abstract
We describe high-throughput protocols that facilitate the selection and characterization of peptide aptamers from yeast dihybrid libraries. These protocols include the preparation and evaluation of the bait fusion and the peptide aptamer screen. They also include confirmation of interaction specific...
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PMID: 19377987
PDF is available here.
PDF is available here.
Abstract
Cell physiology depends on a fine-tuned network of protein-protein interactions, and misguided interactions are often associated with various diseases. Consequently, peptides, which are able to specifically interfere with such adventitious interactions, are of high interest for analytical as well as...
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PMID: 19377990
PDF is available here.
PDF is available here.
Abstract
We have developed a peptide aptamer scaffold protein that can impart small-molecule control over the aptamer-target interaction. This ligand-regulated peptide (LiRP) scaffold, consisting of the protein domains FKBP12, FRB, and GST, binds to the cell-permeable small-molecule rapamycin and the binding...
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PMID: 19377988
PDF is available here.
PDF is available here.
Abstract
Abstract
A novel electrochemical method for the detection of bioaffinity interactions based on a gold-nanoparticles sensing platform and on the usage of stripping voltammetry technique was developed. The oxidation of gold surface (resulted in gold oxide formation) upon polarization served as a basis for anal...
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PMID: 18755579
PDF is available here.
PDF is available here.
Abstract
We show that members of aptameric peptide library constructed within the site 6 and site 8/9 loops of GFP of the ribosome display construct are selectable upon binding to the solid phase IgE antigen. An input of 1.0 microg of the dual site aptameric GFP library exhibiting a diversity of 7.5x10(11) w...
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PMID: 18619414
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (133)9 2008
Abstract
A unique multilabeling at a single-site protocol of the Ru(bpy)(3)(2+) electrochemiluminescence (ECL) system is proposed. Nanoparticles (NPs) were used as assembly substrates to enrich ECL co-reactants of Ru(bpy)(3)(2+) to construct nanoscale-enhanced ECL labels. Two different kinds of NP substrates...
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PMID: 18709196
PDF is available here.
PDF is available here.
Abstract
We isolated PAs able to interact more efficiently with p53 conformational mutants compared with wild-type p53. The interaction between mutant p53 and PAs was further characterized using molecular modeling. Transient expression of PAs was able to reduce the transactivation activity of mutant p53 and...
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PMID: 18701478
PDF is available here.
PDF is available here.
Journal of Molecular Biology (380)5 2008
Abstract
We devised a novel intracellular targeting technique to deliver Tat-fused cargo into the nucleus using an endosome-disruptive peptide (hemagglutinin-2 subunit) and a nuclear localization signal peptide. We show for the first time that Tat-conjugated peptide aptamers can be selectively delivered to t...
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PMID: 18571668
PDF is available here.
PDF is available here.
Chemistry & Biology (15)7 2008
Abstract
We show that a molecular scaffold can be utilized to convert a receptor binding aptamer into a receptor agonist. Many receptors (including tumor necrosis receptor family members) are activated when they are multimerized on the cell surface. Molecular scaffolds have been utilized to assemble multiple...
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PMID: 18635004
PDF is available here.
PDF is available here.
Abstract
We targeted M2-PK by synthetic peptide aptamers, which specifically bind to M2-PK and shift the isoenzyme into its low affinity dimeric conformation. The aptamer-induced dimerization and inactivation of M2-PK led to a significant decrease in the PK mass-action ratio as well as ATP:ADP ratio in the t...
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PMID: 18425820
PDF is available here.
PDF is available here.
Abstract
This paper describes a colorimetric sensing approach for the determination of adenosine triphosphate (ATP) using aptamer-modified gold nanoparticles (Apt-Au NPs). In the absence of the analytes, the color of the Apt-Au NPs solution changed from wine-red to purple as a result of salt-induced aggregat...
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PMID: 18359620
PDF is available here.
PDF is available here.
Abstract
We have utilized a growing concept in systems biology that invokes linear peptide-motifs as a fundamental mechanism driving protein-protein interactions and as a key switch underlying the dynamics of a signal transduction pathway. By using peptide combinatorial libraries as an assay that reflects th...
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PMID: 18376136
PDF is available here.
PDF is available here.
Abstract
We report that a high expression of protein tyrosine kinase 7 (PTK7), a transmembrane receptor protein tyrosine kinase-like molecule, was discovered in a series of leukemia cell lines using whole cell aptamer selection. With the implementation of a two-step strategy (aptamer selection and biomarker...
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PMID: 18363322
PDF is available here.
PDF is available here.
Analytica Chimica Acta (613)2 2008
Abstract
A label-free aptasensor for platelet-derived growth factor (PDGF) protein is reported. The aptasensor uses mixed self-assembled monolayers (SAMs) composed of a thiol-modified PDGF binding aptamer and 6-mercaptohexanol (MCH) on a gold electrode. The SAMs were characterized by cyclic voltammetry (CV),...
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PMID: 18395055
PDF is available here.
PDF is available here.
Electrophoresis (29)7 2008
Abstract
The use of traditional CE to detect weak binding complexes is problematic due to the fast-off rate resulting in the dissociation of the complex during the separation process. Additionally, proteins involved in binding interactions often nonspecifically stick to the bare-silica capillary walls, which...
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PMID: 18324729
PDF is available here.
PDF is available here.
Journal of Molecular Biology (376)4 2008
Abstract
We recently described a new approach for inhibiting IN by "shiftides"--peptides that inhibit the protein by shifting its oligomerization equilibrium from the active dimer to the inactive tetramer. In this study, we used the yeast two-hybrid system with the HIV-1 IN as a bait and a combinatorial pept...
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PMID: 18201721
PDF is available here.
PDF is available here.
Antiviral Research (77)3 2008
Abstract
The peptide aptamers inhibited NS3 protease activity in vitro with an IC50 in the low micromolar range. Upon transfection, aptamers inhibited the replication of SEAP-secreting genotype 1b subgenomic RNA replicons. Aptamer-based intracellular immunization may emerge as a promising antiviral approach...
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PMID: 18243349
PDF is available here.
PDF is available here.
Abstract
Proteomics technologies have produced an abundance of drug targets, which is creating a bottleneck in drug development process. There is an increasing need for better target validation for new drug development and proteomic technologies are contributing to it. Identifying a potential protein drug ta...
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PMID: 18336224
PDF is available here.
PDF is available here.
Analytical Biochemistry (373)2 2008
Abstract
We have developed protein biosensors by using DNA-modified GNPs. We examined how the experimental design, such as the type of interaction between DNA strands and GNPs, temperature, and microenvironment of aptamer, influences the recognition ability of the biosensor. Under our experimental conditions...
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PMID: 18054771
PDF is available here.
PDF is available here.
Abstract
We demonstrate the use of surface-immobilized, oriented peptide aptamers for the detection of specific target proteins from complex biological solutions. These peptide aptamers are target-specific peptides expressed within a protein scaffold engineered from the human protease inhibitor stefin A. The...
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PMID: 18186614
PDF is available here.
PDF is available here.
Abstract
We will give an overview of the mammalian selection systems available, which are based on the expression of peptide aptamers in retroviral or lentiviral vectors. We will show that the isolation and use of peptide aptamers as inhibitors of individual signaling components represents a new challenge fo...
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PMID: 18336207
PDF is available here.
PDF is available here.
Biological Chemistry (389)2 2008
Abstract
We report identification of three putative thiM riboswitches from different bacteria and analysis of their secondary structures. Chemical probing revealed that the riboswitches share similar secondary structures in their aptamer domains that can communicate with the highly variant expression domains...
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PMID: 18163882
PDF is available here.
PDF is available here.
Abstract
We here report on the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution with hydrogen...
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PMID: 18184015
PDF is available here.
PDF is available here.
Abstract
Proteins bear important functions for most life processes. It is estimated that the human proteome comprises more than 250,000 proteins. Over the last years, highly sophisticated and powerful instruments have been developed that allow their detection and characterization with great precision and sen...
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PMID: 17928973
PDF is available here.
PDF is available here.
Abstract
Invasion of mammalian cells by T. cruzi trypomastigotes is a multi-step and complex process involving several adhesion molecules, signaling events and proteolytic activities. From the blood to the cell target in different tissues the parasite has to interact with different cells and the extracellula...
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PMID: 18512341
PDF is available here.
PDF is available here.
Journal of Biology (7)1 2008
Abstract
Peptide aptamers are combinatorial recognition proteins that were introduced more than ten years ago. They have since found many applications in fundamental and therapeutic research, including their recent use in microarrays to detect individual proteins from complex mixtures.
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PMID: 18254928
PDF is available here.
PDF is available here.
Journal of Biology (7)1 2008
Abstract
We present a novel strategy that offers practical solutions to these challenges, featuring peptide aptamers as artificial protein detectors arrayed on gold electrodes with feature sizes one order of magnitude smaller than existing formats. We describe a method to immobilize specific peptide aptamers...
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PMID: 18237447
PDF is available here.
PDF is available here.
Abstract
SERS aptasensors for protein recognition based on Au nanoparticles labeled with aptamers and Raman reporters have been developed, which opens a new way for protein recognition of high sensitivity and selectivity.
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PMID: 18060148
PDF is available here.
PDF is available here.
Biochemistry (Washington) (46)48 2007
Abstract
Anterior gradient-2 protein was identified using proteomic technologies as a p53 inhibitor which is overexpressed in human cancers, and this protein presents a novel pro-oncogenic target with which to develop diagnostic assays for biomarker detection in clinical tissue. Combinatorial phage-peptide l...
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PMID: 17994709
PDF is available here.
PDF is available here.
Abstract
Neural prostheses often suffer from undesired chronic inflammatory tissue response. This can lead to neuronal loss and formation of glial scar tissue, which would serve as a barrier to neural signal transduction. In situ monitoring of neuro-inflammatory cytokines may improve our understanding of dev...
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PMID: 17512185
PDF is available here.
PDF is available here.
Chemical Reviews (107)9 2007
Abstract
Journal of Molecular Biology (371)2 2007
Abstract
We selected peptide aptamers binding to PrP from a combinatorial peptide library presented on the Escherichia coli thioredoxin A (trxA) protein as a scaffold. In a yeast two-hybrid screen employing full-length murine PrP (aa 23-231) as a bait we identified three peptide aptamers that reproducibly bi...
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PMID: 17574575
PDF is available here.
PDF is available here.
Abstract
Abstract
The signal transducer and activator of transcription (STAT) proteins are a family of latent cytoplasmic transcription factors, which form dimers when activated by cytokine receptors, tyrosine kinase growth factor receptors as well as non-receptor tyrosine kinases. Dimeric STATs translocate to the nu...
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PMID: 17216202
PDF is available here.
PDF is available here.
Molecular BioSystems (3)4 2007
Abstract
Protein recognition by synthetic molecules is a challenging endeavour, since these materials must bind to a large relatively flat surface domain and recognize a unique distribution of amino acid residues of varying charge, size and shape. The most promising routes involve specific metal coordination...
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PMID: 17372652
PDF is available here.
PDF is available here.
Abstract
We took advantage of the peptide aptamer strategy to target Nr-13, a Bcl-2 antiapoptotic protein involved in neoplastic transformation by the Rous sarcoma virus. We isolated peptide aptamers that behave as Nr-13 regulators, in vitro and in mammalian cells in culture. Some of these aptamers have pote...
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PMID: 16909120
PDF is available here.
PDF is available here.
Abstract
We use this system to establish a surface plasmon resonance assay offering a limit of detection of 1 nM (150 ng mL-1) and determine the affinity constant of interaction of STM for a cognate antibody to be KD = 1.47 +/- 0.23 nM. Thus, we have established a solid foundation for the future creation of...
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PMID: 17263340
PDF is available here.
PDF is available here.
Journal of Molecular Biology (365)4 2007
Abstract
We have developed a scaffold protein that allows small molecule ligand control over the presentation of a peptide aptamer. This scaffold, a fusion of three protein domains, FKBP12, FRB, and GST, presents a peptide linker region for target protein binding only in the absence of the small molecule Rap...
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PMID: 17116308
PDF is available here.
PDF is available here.
Abstract
We established a lentiviral expression system that allowed the stable expression of the small ABAD-inhibiting peptide by fusion with cytosolic thioredoxin-1 (TRX). The fusion peptide, TA aptamer, was observed within PC12 cytoplasm and maintained both Abeta-binding ability and antioxygenic property s...
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PMID: 17917077
PDF is available here.
PDF is available here.
Abstract
A method for efficient enrichment of protease inhibitors out of a DNA library was developed by introducing SF-link technology. A two-step selection strategy was designed consisting of the initial enrichment of aptamers based on binding function while the second enrichment step was based on the inhib...
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PMID: 17173335
PDF is available here.
PDF is available here.
Abstract
We then showed that CDDP@oxSWNHs/20PEG-NHBP, in which the cancer chemotherapy drug cisplatin (CDDP) was loaded inside the oxSWNHs, was well dispersed in both the PBS and culture medium and exerted a potent cytotoxic effect against cancer cells. The good dispersion of drug-loaded carbon nanomaterials...
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PMID: 17685580
PDF is available here.
PDF is available here.
Abstract
We have initiated a program aiming to develop inhibitors targeting specifically the regulatory CK2beta subunit. Here, we use a two-hybrid approach to isolate from combinatorial libraries, peptide aptamers that specifically interact with CK2beta. One of these (P1), which has significant sequence homo...
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PMID: 16751801
PDF is available here.
PDF is available here.
Abstract
We were able to detect target DNAs by measuring the thrombin activity in a homogeneous solution without bound/free separation, and the lower detection limit was 20 nM....
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PMID: 16876760
PDF is available here.
PDF is available here.
Abstract
We report an alternative approach for more specifically disrupting Smad-dependent signaling using a peptide aptamer, Trx-SARA, which comprises a rigid scaffold, the Escherichia coli thioredoxin A protein (Trx), displaying a constrained 56-amino acid Smad-binding motif from the Smad anchor for recept...
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PMID: 16775010
PDF is available here.
PDF is available here.
Abstract
Fluorescent DNA probes for L-argininamide were developed by a combination of DNA aptamers and fluorophore-quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect L-argininamide specifically. The...
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PMID: 16757168
PDF is available here.
PDF is available here.
Abstract
We developed a method that involves screening DNA aptamers based on their inhibitory activities using an evolution-mimicking algorithm after the pre-selection by SELEX. The value of this method was demonstrated by the identification of an inhibitor of Taq DNA polymerase in a unique single-stranded D...
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PMID: 16815302
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)30 2006
Abstract
We generated PAs that specifically bound to the duck hepatitis B virus (HBV) core protein. Among them, PA34 strongly blocked duck HBV replication by inhibiting viral capsid formation. We found that PA34 led to a dramatic intracellular redistribution of its target protein into perinuclear inclusion b...
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PMID: 16717089
PDF is available here.
PDF is available here.
Journal of Virology (80)12 2006
Abstract
We used the N-terminal domain of TGMV AL1 as bait in a yeast two-hybrid screen of a random peptide aptamer library constrained in the active site of the thioredoxin A (TrxA) gene. The screen selected 88 TrxA peptides that also bind to the full-length TGMV AL1 protein. Plant expression cassettes corr...
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PMID: 16731923
PDF is available here.
PDF is available here.