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Fish Proteins (1):
Recent article
Fish Proteins
Proteomics (11)16 2011
Abstract
We have used 2-DE coupled with MS to identify proteins differentially expressed in the livers of juvenile gilthead sea bream (Sparus aurata) grown under fish-farm conditions and fed with a 100 mg/kg MA-enriched diet (MA(100)). After the comparison of the protein profiles from MA(100) fed fish and...
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PMID: 21751345
PDF is available here.
PDF is available here.
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Abstract
E3 ubiquitin ligases are central for the selection of proteins targeted for degradation by the ubiquitin proteasome pathway. In this study atrogin-1 (Fbox-32), a major E3 ligase in muscle, has been characterized in Atlantic salmon (Salmo salar). The protein sequence is highly conserved between teleo...
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PMID: 20728561
PDF is available here.
PDF is available here.
Abstract
We examined the RAS and NP system in the euryhaline Atlantic stingray, Dasyatis sabina (Lesueur). To determine the relative sensitivity of target organs to these hormonal systems, we isolated cDNA sequences encoding the D. sabina angiotensin receptor (AT) and natriuretic peptide type-B receptor (NPR...
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PMID: 20869458
PDF is available here.
PDF is available here.
Abstract
We found LAO activity in the serum of rockfish Sebastes schlegeli. The LAO was isolated from the serum by sequential column chromatography of Con-A lectin affinity chromatography, anion exchange HPLC, hydroxyapatite HPLC and gel filtration HPLC, and characterized. The LAO (a molecular mass of 160 kD...
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PMID: 20728563
PDF is available here.
PDF is available here.
Abstract
We have identified one sox11 homologue, sox11b, from the ovary of the orange-spotted grouper. In the present study, another sox11 homologue, sox11a, was cloned from the brain. The orange-spotted grouper Sox11a contained the signature features of mammalian SOX11 homologues except the Pro-Glu rich reg...
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PMID: 20851206
PDF is available here.
PDF is available here.
Abstract
In the present study, a full-length cDNA encoding the insulin-like growth factor binding protein-2 (IGFBP-2) was cloned from the liver of orange-spotted grouper (Epinephelus coioides) by rapid amplification of cDNA ends technique. The IGFBP-2 cDNA sequence was 1413 bp long and had an open reading fr...
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PMID: 20708707
PDF is available here.
PDF is available here.
Abstract
Our results suggest that NPY mRNA levels in Brazilian flounder are affected by temperature....
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PMID: 20826950
PDF is available here.
PDF is available here.
Abstract
Biotechnology Letters (32)8 2010
Abstract
Expression of four reference genes of grass carp, including beta-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S rRNA (18S) and elongation factor-1 alpha (EF1alpha), was studied in tissues of normal individuals and bacteria-infected individuals. EF1alpha had the most stable expre...
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PMID: 20361234
PDF is available here.
PDF is available here.
Abstract
Our present results, including seasonality, slightly differ from former observations (Solé et al., 2009a) and reveal a more significant role of the ecological variables in controlling biomarkers expression in fish from the shelf....
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PMID: 20494437
PDF is available here.
PDF is available here.
Abstract
To investigate the influence of N- or C-terminal regions of pleurocidin (Ple) peptide on the antifungal activity, four analogues partially truncated in the N- or C-terminal regions were designed and synthesized. Circular dichroism (CD) spectroscopy demonstrated that all the analogues maintained an a...
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PMID: 20798581
PDF is available here.
PDF is available here.
Journal of Comparative Neurology (518)14 2010
Abstract
We show that the electric fish Apteronotus leptorhynchus can learn to recognize individual conspecifics based on a simple cue, the beat frequency of their summed sinusoidal electric organ discharges (EOD). Male fish produce transient communication signals (chirps) in response to mimic EODs. The chir...
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PMID: 20506470
PDF is available here.
PDF is available here.
Abstract
We show that two zebrafish proteins, which we name actinodin 1 and 2 (And1 and And2), are essential structural components of elastoidin. The presence of actinodin sequences in several teleost fishes and in the elephant shark (Callorhinchus milii, which occupies a basal phylogenetic position), but no...
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PMID: 20574421
PDF is available here.
PDF is available here.
Abstract
We previously isolated from the plasma of Fugu pardalis (Yotsu-Yamashita et al., 2001). For the study on functions of PSTBP, here we examined distribution of homologous proteins to PSTBP in the plasma of seven species of puffer fish, and among the tissues of F. pardalis by Western blot analysis prob...
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PMID: 20043937
PDF is available here.
PDF is available here.
Abstract
I vitellogenin. In addition to the liver of the female fish, wcmmVtg was also shown to be expressed in the ovary. During ovarian development, the mRNA expression of wcmmVtg in both the liver and ovary was continuously increased from the previtellogenic to late vitellogenic stages, but then decreased...
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PMID: 20467857
PDF is available here.
PDF is available here.
Isolation of Tanichthys albonubes beta actin gene and production of transgenic Tanichthys albonubes.
Abstract
A beta actin cDNA of Tanichthys albonubes was isolated through the RT-PCR and RACE approach. The cDNA was 1,787-bp in length, including a 1,128-bp CDS, a 95-bp 5'UTR and a 564-bp 3'UTR. Genomic DNA containing the transcription region and 5'-flanking region was cloned based on the beta actin cDNA by...
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PMID: 20467859
PDF is available here.
PDF is available here.
Abstract
We have found that grouper expression of sex-determining region Y-related high-mobility group-box gene 3 (SOX3) is a significant time point for enterable gametogenesis of primordial germ cells, because SOX3 is obviously expressed and localized in primordial germ cells. As SOX3 continues to express,...
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PMID: 20467860
PDF is available here.
PDF is available here.
Abstract
These results indicated KC70 plays important and multiple roles in turbot immune response....
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PMID: 20467856
PDF is available here.
PDF is available here.
Abstract
We used reverse transcription and rapid amplification of cDNA ends (RACE) to obtain the three full-length cDNA sequences encoding GH and two forms of IGF-I from the giant grouper (Epinephelus lanceolatus), a coral fish of high commercial value cultured in Southeast Asia. GH precursor cDNA consists o...
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PMID: 20467861
PDF is available here.
PDF is available here.
Abstract
In this study the effects of growth hormone (GH) on silver sea bream branchial heat-shock protein 70 (HSP70) expression was investigated using in-vivo and in-vitro experiments. For in-vivo experiments, sea bream were administered recombinant bream GH or the GH secretagogue hexarelin. Pituitary level...
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PMID: 20467854
PDF is available here.
PDF is available here.
Abstract
We investigate comparative brain development in ecologically distinct cichlid fishes from Lake Malawi and demonstrate that brains vary among recently evolved lineages because of early patterning. Divergence among rock-dwellers and sand-dwellers in the relative size of the telencephalon versus the th...
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PMID: 20439726
PDF is available here.
PDF is available here.
Abstract
The responses of Mozambique and Nile tilapia acclimated to fresh water (FW) and brackish water (BW; 17 per thousand) were compared following acute salinity challenges. In both species, plasma osmolality increased to above 450 mOsm by 2h after transfer from FW to seawater (SW); these increases in osm...
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PMID: 20138183
PDF is available here.
PDF is available here.
Abstract
Proteases in fish muscle often cause undesired softening of intact meat pieces during refrigerated storage or slow cooking. Several food-grade proteinaceous inhibitors can overcome this softening if properly delivered to the intracellular sites where proteases are located. Fluorescence recovery afte...
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PMID: 20380449
PDF is available here.
PDF is available here.
Molecular Immunology (47)9 2010
Abstract
We have studied the IFN response in rainbow trout (Oncorhynchus mykiss) ovary using two viruses with different replicative capacity in this organ, namely viral haemorrhagic septicaemia virus (VHSV) and infectious pancreatic necrosis virus (IPNV). Both VHSV and IPNV are shed from the ovary, but while...
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PMID: 20356627
PDF is available here.
PDF is available here.
Journal of Experimental Biology (213)Pt 7 2010
Abstract
We analyzed the transport properties of trout Rh proteins (Rhag, Rhbg1, Rhbg2, Rhcg1, Rhcg2, Rh30-like) expressed in Xenopus oocytes, using the radiolabeled ammonia analogue [(14)C]methylamine, and the scanning ion electrode technique (SIET). All of the trout Rh proteins, except Rh30-like, facilitat...
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PMID: 20228341
PDF is available here.
PDF is available here.
Abstract
We exposed the dogfish to Ni (587microg/L), Cd (1124microg/L), Pb (2072microg/L), Cu (635microg/L), and Ag (1079microg/L and two additional exposures at 10microg/L and 1microg/L) for one week and measured total metal accumulation, metallothionein induction, and parameters related to osmoregulation....
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PMID: 20044020
PDF is available here.
PDF is available here.
Abstract
We detected the presence of the recently discovered piscidin 4 via bug blot, Western blot, ELISA and/or immunohistochemistry in striped bass (Morone saxatilis), white bass (M. chrysops), European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), red drum (Sciaenops ocellatus), and b...
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PMID: 19913049
PDF is available here.
PDF is available here.
Abstract
We describe a new method for sequencing-based cross-species transcriptome comparisons and define a new metric for evaluating gene expression across species using protein-coding families as units of comparison. Using this measure transcriptomes from different species were evaluated by mapping them to...
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PMID: 20347830
PDF is available here.
PDF is available here.
Abstract
We obtained a SLalpha gene from black seabream (bsb), with its 5' flanking promoter region carrying several putative transcription factors including seven binding sites for pituitary-specific transcription factor 1 (Pit-1). To study the actions of Pit-1 on this gene promoter, we cloned three variant...
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PMID: 19766121
PDF is available here.
PDF is available here.
Abstract
In the present study, tyrosine hydroxylase (TH, the rate-limiting enzyme in catecholamine synthesis) activity was demonstrated in the ovary of the catfish to elucidate the possible physiological role of catecholamines in the gonad. The ovary is innervated by seven pairs of nerves, originating from t...
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PMID: 20004203
PDF is available here.
PDF is available here.
Abstract
Our results suggest that leptin has an acute effect on the regulation of food intake, energy expenditure and lipid metabolism in grass carp, but the effect can be rapidly counteracted through mechanisms that are currently unknown.
Copyright 2009 Elsevier Inc. All rights reserved....
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PMID: 19857495
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)3 2010
Abstract
Seventeen nucleotide sequences of Co-1 gene from 13 Pleuronectiformes species and 2 Perciformes species served as the outgroup were examined. For divergence comparison, the initial stage involved calculation of pairwise p-distances for all investigated sequences. This allowed to evaluate the nucleot...
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PMID: 20391785
PDF is available here.
PDF is available here.
Abstract
We report the first three-dimensional structure of Pa4 bound to LPS micelles. The binding kinetics of Pa4 with LPS is estimated using [(15)N-Leu-19] relaxation dispersion NMR experiments. LPS/Pa4 interactions are further characterized by a number of biophysical methods, including isothermal titratio...
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PMID: 19959835
PDF is available here.
PDF is available here.
Abstract
We have performed molecular dynamics simulations of the interaction of Piscidin (Pis1) and its two analogues (Pis1-AA and Pis1-PG) with dipalmitoylphosphatidylcholine (DPPC) bilayer through 45 ns. Our results inform us of the detailed location and orientation of the peptide with respect to the bilay...
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PMID: 19916575
PDF is available here.
PDF is available here.
Zoological Research (31)1 2010
Abstract
The creatine kinase (CK) cDNA from the mandarin fish Siniperca chuatsi was cloned by RT-PCR and rapid amplification of cDNA ends (RACE) methods. The structural characteristics and phylogeny of this gene were analyzed. Sequence analysis revealed a 1586 bp cDNA sequence containing 92 bp 5'-untranslate...
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PMID: 20446457
PDF is available here.
PDF is available here.
Abstract
At selected sites around the UK, the offshore sentinel flatfish species dab Limanda limanda are found to contain elevated levels of macroscopic liver tumors. Previous proteomic and metabolomic studies have demonstrated that differences exist between tumor and non-tumor tissues; however, these differ...
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PMID: 20225674
PDF is available here.
PDF is available here.
Abstract
Data are presented about the presence of serum albumins in fishes of different classes and orders inhabiting different ecological conditions, about structure of typical albumins and albumin-like proteins, and about the degree of homology of these proteins to mammalian albumins. There is shown a wide...
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PMID: 20432704
PDF is available here.
PDF is available here.
Abstract
Apolipoproteins play important roles in lipid transport and uptake in vertebrates, and they are associated with pathogenesis of many cardiovascular diseases. However, the diverse apolipoproteins in individual fish species have not been extensively characterized. Partial cDNA sequence...
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PMID: 19800026
PDF is available here.
PDF is available here.
Folia Biologica (Krakow) (58)1-2 2010
Abstract
DNA fragments related to the cloudy catshark Scyliorhinus torazame L-gulono-gamma-lactone oxidase (GULO) cDNA were detected in a distant fish species. Although the Southern hybridization pattern was more distinct in species with active GULO, DNA fragments related to the GULO gene were also discovere...
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PMID: 20420195
PDF is available here.
PDF is available here.
Abstract
These results will greatly contribute to our understanding of the effects of fluoride exposure on the physiological and biochemical functions of Takifugu and the toxicological mechanism of fluoride causing fluorosis in both fish and human....
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PMID: 20146379
PDF is available here.
PDF is available here.
British Journal of Nutrition (102)11 2009
Abstract
The aim of the present study was to analyse the effects of partial or total replacement of fish meal (FM) and fish oil (FO) by a mixture of plant protein (PP) and a mixture of vegetable oils (VO) on the hepatic insulin-nutrient-signalling pathway and intermediary metabolism-related gene expression i...
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PMID: 19664314
PDF is available here.
PDF is available here.
Abstract
Proteome analysis was used to study the effects of probiotic bacteria treatment on the protein expression in early Atlantic cod (Gadus morhua) larvae. The main focus was on proteins connected to immune function, growth and development. The results demonstrated that none of the identi...
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PMID: 20403755
PDF is available here.
PDF is available here.
Abstract
We investigated the effect of salinity on trunk kidney proteome in ayu (Plecoglossus altivelis) using two-dimensional gel electrophoresis and mass spectrometry. The proteins involved in the process of energy metabolism, biosynthesis, DNA methylation and cell differentiation were mainly affected, and...
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PMID: 20403753
PDF is available here.
PDF is available here.
Current Eye Research (34)11 2009
Abstract
A proteomics approach has been used to identify differentially expressed proteins in form-deprived combined retinal, RPE, and choroidal tissue from myopic versus normal eyes. The identified proteins may be components of pathways involved in myopia pathogenesis....
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PMID: 19958113
PDF is available here.
PDF is available here.
Abstract
The moisture, ash, protein and fat content and amino acid, fatty acid and mineral compositions in the flesh of three commercially important fish species (Dicentrarchus labrax, Sparus aurata, Dentex dentex) from the aqua culture of Turkey were evaluated. Ranges of moisture, ash, p...
PDF is available here.
PDF is available here.
Abstract
A full length cDNA encoding the growth hormone receptor (GHR) of Chinese sturgeon was cloned in order to investigate the mechanism of growth hormone in regulating the growth of Chinese sturgeon. The open reading frame of the cloned Chinese sturgeon growth hormone receptor (csGHR) cDNA encodes a tran...
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PMID: 19911126
PDF is available here.
PDF is available here.
British Journal of Nutrition (102)6 2009
Abstract
The purpose of the present study was to investigate whether water-insoluble fish protein (IFP) from Alaska pollock (Theragra chalcogramma) prevents hypercholesterolaemia induced by ovarian hormone deficiency. Wistar female rats, aged 6 months, were subjected to sham-operation or ovariectomy, and fed...
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PMID: 19335928
PDF is available here.
PDF is available here.
Abstract
As part of a continuing search for potential anticancer drug candidates from antimicrobial peptides of marine organisms, tilapia (Oreochromis mossambicus) hepcidin TH2-3 was evaluated in several tumor cell lines. The results indicated that TH2-3, a synthetic 20-mer antimicrobial peptide, specificall...
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PMID: 19539000
PDF is available here.
PDF is available here.
Stem Cells (27)9 2009
Abstract
We have identified the homolog of the mammalian Nanog gene in teleost fish and describe the endogenous expression of Ol-Nanog mRNA and protein during medaka (Oryzias latipes) embryonic development and in the adult gonads. Using medaka fish as a vertebrate model to study Nanog function, we demonstrat...
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PMID: 19544407
PDF is available here.
PDF is available here.
Abstract
Trypsin from the pyloric ceca of pectoral rattail (Coryphaenoides pectoralis) was purified and characterized. Purification was carried out by ammonium sulfate precipitation, followed by column chromatographies on Sephacryl S-200, DEAE-cellulose and Sephadex G-50. The enzyme was purified 89-fold with...
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PMID: 19585993
PDF is available here.
PDF is available here.
Abstract
The capacity of Atlantic pollock ( Pollachius pollachius ), seabass ( Dicentrarchus labrax ), and horse mackerel ( Trachurus trachurus ) hemoglobin (Hb) to promote lipid oxidation has been evaluated in liposomes and washed minced horse mackerel muscle. The pro-oxidant ability of fish Hbs was related...
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PMID: 19722582
PDF is available here.
PDF is available here.
Abstract
We previously reported a frame-shift mutation on SLa in a medaka mutant, color interfere (ci), which shows defects in body-color regulation, lipid storage, and cortisol secretion. In this study, we examined the effects of introducing a DNA fragment which constitutively expresses wild-type SLa under...
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PMID: 19393303
PDF is available here.
PDF is available here.
Abstract
The complement system is one of the first line of immune defence mechanisms as well as a modifier of acquired immunity. C3 is the central complement component primarily synthesized in liver. The local synthesis of C3 in tissues other than liver may play an important role in local inflammatory proces...
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PMID: 19775074
PDF is available here.
PDF is available here.
Abstract
We collected 12 kinds of fish cytokines and constructed their respective consensus sequences using hidden Markov models; then, the conserved domains region of each consensus sequence were further extracted by the SMART tool, and used as the query sequence for PSI-BLAST analysis in Drosophila melanog...
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PMID: 19514841
PDF is available here.
PDF is available here.
Abstract
We identified slo1 genes in teleost and nonteleost fishes using polymerase chain reaction and genetic sequence databases. In contrast to tetrapods, all teleosts examined were found to express duplicate slo1 genes in the central nervous system, whereas nonteleosts that diverged prior to the teleost w...
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PMID: 19321796
PDF is available here.
PDF is available here.
Abstract
Protein recovery for fish processing by-products utilizes extreme pH shifts for isoelectric solubilization and precipitation. The purpose of this study was to determine if Escherichia coli would survive exposure to the extreme pH shifts during the protein recovery process. Fresh rainbow trout were b...
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PMID: 19681261
PDF is available here.
PDF is available here.
Abstract
This study was conducted to investigate time-dependent changes in oxidative enzymes in liver of crucian carp after intraperitoneally injection with extracted microcystins 600 and 150 microg kg(-1) body weight. The results showed that activities of antioxidant enzymes, including superoxide dismutase,...
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PMID: 19252760
PDF is available here.
PDF is available here.
Abstract
We also compared changes in select proteins to changes in their mRNA levels and observed, in general, that proteins and mRNA changes did not correlate, suggesting complex regulation at the level of both the transcriptome and proteome. It is concluded that both transcriptomic and proteomic approaches...
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PMID: 19267455
PDF is available here.
PDF is available here.
Immunogenetics (61)4 2009
Abstract
We have examined gene expression of the trout 2C type I IFN in cultured cells following stimulation with lipopolysaccharide, phytohaemagglutinin, polyI:C or recombinant IFN, or after transfection with polyI:C. The kinetics of gene expression was also studied after viral infection. Analysis of the re...
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PMID: 19288092
PDF is available here.
PDF is available here.
Aquatic Toxicology (92)1 2009
Abstract
Tributyltin (TBT), widely used as an antifouling biocide, is the most abundant pesticide in coastal environments. Trimethyltin (TMT) is a potent neurotoxicant of a mechanism of action yet to be uncovered. The neurotoxicity of TBT and TMT on the brain of marine fish Sebastiscus marmoratus was investi...
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PMID: 19223082
PDF is available here.
PDF is available here.