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Insect Proteins (3):
Recent article
Insect Proteins
Biomacromolecules (12)6 2011
Abstract
We have designed and produced a modular, recombinant resilin-like polypeptide bearing both mechanically active and biologically active domains to create novel biomaterial microenvironments for engineering mechanically active tissues such as blood vessels, cardiovascular tissues, and vocal folds. Pre...
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PMID: 21553895
PDF is available here.
PDF is available here.
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Abstract
We previously examined the functional roles of adipokinetic hormone in Manduca sexta L. (Lepidoptera: Sphingidae). Here we report the cloning of the full-length cDNA encoding the putative adipokinetic hormone receptor from the fat body of M. sexta. The sequence analysis shows that the deduced amino...
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PMID: 21529255
PDF is available here.
PDF is available here.
Abstract
These results suggest that Cu/ZnSOD may play a larger role than MnSOD as a superoxide anion scavenger against oxidative stress in H. cunea.
Copyright © 2010 Elsevier Inc. All rights reserved....
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PMID: 20728562
PDF is available here.
PDF is available here.
Microbial Pathogenesis (49)6 2010
Abstract
These results demonstrate that rational design virulence factor is a potential strategy for strain improvement by genetic engineering.
Copyright © 2010. Published by Elsevier India Pvt Ltd....
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PMID: 20674735
PDF is available here.
PDF is available here.
Development (137)22 2010
Abstract
We report that although Nv nanos (Nv-nos) has a conserved function in embryonic patterning through translational repression of hb, the timing and mechanisms of this repression are significantly delayed in the wasp compared with the fly. This delay in Nv-nos function appears to be related to the dyna...
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PMID: 20929949
PDF is available here.
PDF is available here.
Abstract
This article describes the dissection of early Drosophila embryos to generate flat or fillet preparations. For the procedure, a modified chamber or well is designed using glass slides and sealant. Embryos are dissected by removing the chorionic (outer) membrane (performed outside the chamber) and th...
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PMID: 20889694
PDF is available here.
PDF is available here.
Abstract
We screened the recently sequenced genome of the parasitic wasp, Nasonia vitripennis, for the presence of these signaling molecules and annotated 30 precursor genes encoding 51 different mature neuropeptides or protein hormones. Twenty-four of the predicted mature Nasonia neuropeptides could be expe...
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PMID: 20695486
PDF is available here.
PDF is available here.
Abstract
We evidenced that pheromone biosynthesis towards the immediate dienoic gland precursor, the (E,Z)-6,11-hexadecadienoic acid, involves desaturation steps with Δ(6) and Δ(11) regioselectivity. cDNA cloning of pheromone gland desaturases and heterologous expression in yeast demonstrated that the 6,11...
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PMID: 20691782
PDF is available here.
PDF is available here.
Abstract
We have studied the hyperactive insect AFP from Tenebrio molitor (TmAFP) with the (17)O magnetic relaxation dispersion (MRD) method, which selectively monitors the rotational motion and exchange kinetics of water molecules on picosecond-microsecond time scales. The global hydration behavior of TmAFP...
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PMID: 20668761
PDF is available here.
PDF is available here.
Abstract
We identified host proteins that changed in response to host cell susceptibility to baculovirus infection. We used three baculovirus-host cell systems utilizing two cell lines derived from pupal ovaries, Hz-AM1 (from Helicoverpa zea) and Hv-AM1 (from Heliothis virescens). Hv-AM1 cells are permissive...
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PMID: 20362582
PDF is available here.
PDF is available here.
Abstract
We for the first time report the role of ecdysteroids in the regulation of hexamerin synthesis in a lepidopteran insect Corcyra cephalonica. The hormonal studies were carried out using the normal and the thorax-ligated insects with both 20E and its non-steroidal agonist RH-5992. The in vitro as well...
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PMID: 20361975
PDF is available here.
PDF is available here.
Abstract
We used stable isotopes to quantify the allocation of capital and income resources to reproduction in the flesh fly, Sarcopha crassipalpis and assessed how allocation patterns change over multiple bouts of reproduction. Sarcophaga crassipalpis shifts from a slight investment of capital in the first...
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PMID: 20417214
PDF is available here.
PDF is available here.
Abstract
Comparative molecular analysis reveals a wide variation of clock mechanisms among insects. In this study, the clock gene homologues of Clock (Td'Clk) and cycle (Td'cyc) were cloned from an apterygote insect, Thermobia domestica. Structural analysis showed that Td'CLK includes bHLH, PAS-A, PAS-B doma...
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PMID: 20416313
PDF is available here.
PDF is available here.
Protein degradation in silkworm peritracheal athrocytes and its physiological role in metamorphosis.
Abstract
We detected the uptake and degradation of foreign proteins in peritracheal athrocytes by immunohistochemical, Western blot, and ex vivo analyses. IgG-FITC was detected in the athrocytes of silkworm larvae following injection, and LysoTracker analysis showed endosomal and lysosomal colocalizations. A...
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PMID: 20230827
PDF is available here.
PDF is available here.
Abstract
We also analyzed other hexamerin sequestering tissues like salivary gland, male accessory reproductive gland and ovary for the presence of hexamerin receptor. We found that the receptor in these tissues was similar in size and mode of activation to that of fat body hexamerin receptor, thus cementing...
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PMID: 20230828
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)9 2010
Abstract
Different genome regions differ in replication timing during the S phase. Late-replicating sequences are often underreplicated in the Drosophila salivary-gland polytene chromosomes. The SuUR gene, whose mutation changes the replication time of late-replicating regions in salivary-gland cells, has be...
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PMID: 21061633
PDF is available here.
PDF is available here.
Abstract
We detected several long chain aliphatic alcohols ranging from C18-C32 in honey bees and the level of these alcohols varied in each body segment. C18:0Alc and C20:0Alc are more pronounced in the head, whereas C22:0Alc to C32Alc are abundant in the abdomen. One of the cDNAs coding for a fatty acyl-Co...
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PMID: 20542116
PDF is available here.
PDF is available here.
Abstract
Levels of juvenile hormone III (JH), FGLamide allatostatin peptides (ASTs), ASTs precursor (preproAST) mRNA and methyl farnesoate epoxidase (CYP15A1) mRNA were measured in embryos of the cockroach Blattella germanica. JH starts to rise just after dorsal closure, reaches maximal levels between 60% an...
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PMID: 20542115
PDF is available here.
PDF is available here.
Abstract
Our data reveal that the mechanism by which a multiligand receptor recognizes microorganisms contributes substantially to their phagocytosis by hemocytes. A better understanding of an opsonin with the required repertoire for detecting diverse invaders might provide us with critical insights into the...
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PMID: 20519517
PDF is available here.
PDF is available here.
Abstract
Although many antimicrobial components (i.e. antimicrobial peptides) have been found in many social Hymenoptera venoms, no antimicrobial compound is purified and characterized from parasitic Hymenoptera. From the venoms of the ectoparasitic wasp, Nasonia vitripennis, a defensin-like antimicrobial pe...
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PMID: 20362606
PDF is available here.
PDF is available here.
Abstract
To obtain the cDNA sequence encoding fibrinolytic enzyme from Eupolyphaga sinensis and express it in prokaryotic and eukaryotic expression system.
The primers were designed according to the cDNA of other animals'fibrinolytic enzyme. The cDNA sequence was cloned by RT-PCR and 3 RACE.
Sequence analysi...
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PMID: 20931838
PDF is available here.
PDF is available here.
Abstract
We compared the luminescence and catalytic properties of a recently cloned luciferase-like enzyme from Zophobas mealworm, an AMP/CoA-ligase displaying weak luminescence activity, with those of cloned luciferases from the three main families of luminescent beetles: Phrixthrix hirtus railroad worm; Py...
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PMID: 20526507
PDF is available here.
PDF is available here.
Journal of Biochemistry (148)2 2010
Abstract
We cloned and characterized four CSP genes from Plutella xylostella. The deduced amino acid sequences of PxylCSP1, PxylCSP2, PxylCSP3 and PxylCSP4 revealed open reading frames of 152, 128, 126 and 126 amino acids, respectively, with four conserved cysteine residues. The expression patterns of the fo...
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PMID: 20495011
PDF is available here.
PDF is available here.
Abstract
We determined the tissue distribution of a recently cloned adenylyl cyclase (AmAC3) in honeybee brain. The protein is present in all neuropils. Intensive immunoreactivity was found in parts of the proto- and deutocerebrum and in the suboesophageal ganglion. Biochemical and pharmacological properties...
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PMID: 20685336
PDF is available here.
PDF is available here.
Abstract
We focus on the new hemimetabolous paradigm. We highlight how hemimetabolous short-germ or intermediate-germ embryos establish the anterior/posterior (A/P) pattern and the importance of dynamic cell movement during germband formation. In hemimetabolous insects, orthodenticle, encoding a homeodomain-...
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PMID: 20462751
PDF is available here.
PDF is available here.
Abstract
We report results of similar studies testing blood uptake of horn flies feeding on a natural host, cattle. These studies confirmed the association of ts genotype with blood uptake of horn flies and showed that it was host species specific. In contrast to rabbits, blood uptake volumes of homozygous t...
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PMID: 20695276
PDF is available here.
PDF is available here.
Acta Crystallographica Section F (66)Pt 7 2010
Abstract
Cecropin B is a 37-residue cationic antimicrobial peptide derived from the haemolymph of Bombyx mori. The precise mechanism by which cecropins exert their antimicrobial and cytolytic activities is not well understood. Crystals of cecropin B were obtained by the hanging-drop vapour-di...
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PMID: 20606290
PDF is available here.
PDF is available here.
Abstract
We present for the first time a thorough structural characterization of these allergens, which have recently been tested to induce an increase of allergy specific indicators in blood of Europeans. We isolated two Per a 3 isoforms, which occur freely dissolved in the hemolymph as hexamers with molecu...
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PMID: 20100511
PDF is available here.
PDF is available here.
Abstract
We report the cloning of a cDNA from the honeybee (Am5-ht1A) sharing high similarity with members of the 5-HT(1) receptor class. Activation of Am5-HT(1A) by serotonin inhibited the production of cAMP in a dose-dependent manner (EC(50) = 16.9 nM). Am5-HT(1A) was highly expressed in brain regions know...
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PMID: 20349263
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)3 2010
Abstract
To express the genes of adenosine deaminase (ADA), C-lectin and serpin (serine protease inhibitor) in the salivary gland of Aedes albopictus.
Total RNA was extracted respectively from salivary glands of unfed (group SG) and engorged adult female Ae. albopictus mosquitoes (group BSG), female carcasse...
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PMID: 20806502
PDF is available here.
PDF is available here.
Abstract
We report the identification of the gene responsible for the Green b (Gb) locus involving the green cocoon trait. In +(Gb) animals, glucosylation at the 5-O position of dietary quercetin did not occur, and the total amount of flavonoids in tissues and cocoons was dramatically reduced. We performed p...
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PMID: 20534444
PDF is available here.
PDF is available here.
Abstract
Abstract
To search for novel transcripts encoding biologically active venom components, a subtractive cDNA library specific to the venom gland and sac (gland/sac) of a solitary hunting wasp species, Eumenes pomiformis Fabricius (1781), was constructed by suppression subtractive hybridization. A total of 541...
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PMID: 20096300
PDF is available here.
PDF is available here.
Inflammation (33)3 2010
Abstract
We used an acute inflammation model of injury, injection of carrageenan into the pleural cavity of rats. The rats were treated intraperitoneally with the aqueous extract of U. dermestoides 8 and 16 mg/kg. The exudate volume, protein concentration, polymorphonuclear leukocytes (PMNs) and total leukoc...
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PMID: 20020191
PDF is available here.
PDF is available here.
Bioscience Reports (30)3 2010
Abstract
We firstly characterized the proteome of cultured BmN cells, derived from Bombyx mori. Total 1478 proteins were identified with two or more peptides by using 1D (one-dimensional) SDS/PAGE and LTQ-Orbitrap. According to the gene ontology annotation, these proteins presented diverse pI values and mole...
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PMID: 19496755
PDF is available here.
PDF is available here.
Abstract
We have cloned the cDNA of the first receptor known to be involved in an antidiuretic strategy in insects, a strategy that prevents diuresis. This receptor belongs to the insect CAPA receptor family known in other insects to be activated by peptides encoded within the capability gene. We characteriz...
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PMID: 20479227
PDF is available here.
PDF is available here.
Abstract
By using shotgun HPLC-ESI-MS proteomics approach, 2043 peptides were identified from the midgut of Spodoptera litura larvae at the sixth instar feeding stage, out of which 1489 (72.9%) were found to have their homologues in the public protein databases and 842 had identities of molec...
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PMID: 20345177
PDF is available here.
PDF is available here.
Abstract
We compared royal jelly (RJ) produced by Apis mellifera ligustica and Apis cerana cerana in production, protein profiles, and abundances using proteomic approaches. The RJ proteome was displayed using two-dimensional gel electrophoresis (2DGE), and proteins were identified using MALDI-TOF MS and LC-...
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PMID: 20307072
PDF is available here.
PDF is available here.
Abstract
We subjected first instar larvae, 16-h old pupae and female of Aedes aegypti (L.) (Diptera: Culicidae) to heat shock treatment for 0, 15, 30, 60, and 180 min at 23 and 42 degrees C. The heat shock protein genes AeaHsp26, AeaHsp83, and AeaHsc70 were examined by comparing relative transcript expressio...
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PMID: 20496584
PDF is available here.
PDF is available here.
Biotechnology Letters (32)5 2010
Abstract
We developed a cecropin B mutant with a green fluorescent protein fusion partner resulting in high expression of a 37 kDa fusion peptide in E. coli with a yield of 7.9 mg/l culture medium after purification on Ni-IDA resin. Guinea pigs when immunized with the fusion peptide produced a specific antis...
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PMID: 20047068
PDF is available here.
PDF is available here.
Toxicological Sciences (115)1 2010
Abstract
We used the uncorrelated shrunken centroid method to classify gene expression profiles of the springtail Folsomia candida exposed to soil spiked with six different metals (barium, cadmium, cobalt, chromium, lead, and zinc). We identified a gene set (classifier) of 188 genes that can discriminate bet...
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PMID: 20133373
PDF is available here.
PDF is available here.
Abstract
We examined the expression of dorsoventral (DV) boundary candidate genes in developing wings of several species of Lepidoptera. We found that the transcription factor Cut and mRNA for the signaling molecule wingless (wg) are strongly co-expressed in a discrete zone around the larval wing disc margin...
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PMID: 20565540
PDF is available here.
PDF is available here.
Abstract
We compared the expression pattern of eight pigment-associated genes between three Papilio species. The spatial expression pattern of melanin-related genes coincided with the species-specific cuticular markings. We newly found that the combination of bilin-binding protein and yellow-related gene (YR...
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PMID: 20565541
PDF is available here.
PDF is available here.
Abstract
These results suggest the possibility that Bla g 5 may have a conformational epitope in the C-terminal region. GST is the important target for the development of vaccines and drugs against allergic diseases because of high cross-reactivity among insect species. This study will aid recombinant allerg...
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PMID: 20044919
PDF is available here.
PDF is available here.
Abstract
We therefore decided to investigate the expression of OBPs and CSPs in the honeybee at the protein level, using a proteomic approach. Our results are in agreement with previous reports of expression at the RNA level and show that 12 of the 21 OBPs predicted in the genome of the honeybee Apis mellife...
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PMID: 20155982
PDF is available here.
PDF is available here.
Abstract
We discuss recent findings on the role of the mosquito's innate immune signaling pathways in preventing infection by the Plasmodium parasite, the identification and mechanistic description of novel anti-parasite molecules, the role that natural bacteria harbored in the mosquito midgut might play in...
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PMID: 20026176
PDF is available here.
PDF is available here.
Abstract
We sequenced the complete mitochondrial genome of Evania appendigaster. This genome is 17,817 bp long; with low A+T content, 77.8%, compared with other hymenopteran species. Four tRNA genes were rearranged, among which remote inversion is the dominant gene rearrangement event. Gene shuffling is caus...
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PMID: 19655273
PDF is available here.
PDF is available here.
Abstract
I from the moth Heliothis zea. By fluorescence emission measurements it was shown that both fluorescent helicokinin I analogues react sensitive on the dielectricity of their microenvironment. A helicokinin I analogue containing the fluorescent tryptophan mimic beta-[6'-(N,N-dimethyl)-amino-2'-naphth...
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PMID: 19995343
PDF is available here.
PDF is available here.
Abstract
We show that a peroxidase, secreted by the Anopheles gambiae midgut, and dual oxidase form a dityrosine network that decreases gut permeability to immune elicitors. This network protects the microbiota by preventing activation of epithelial immunity. It also provides a suitable environment for malar...
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PMID: 20223948
PDF is available here.
PDF is available here.
Abstract
Two series of new nitromethylene neonicotinoid analogues (2a-2h and 3a-3h) were designed and prepared, with the cis-configuration confirmed by X-ray diffraction. Preliminary bioassays showed that most analogues exhibited excellent insecticidal activities at 500 mg/L, and analogues with optical activ...
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PMID: 20180506
PDF is available here.
PDF is available here.
Abstract
Triatoma (T.) dimidiata is a hematophagous Hemiptera and a main vector of Chagas disease. The saliva of this and other blood-sucking insects contains potent pharmacologically active components that assist them in counteracting the host hemostatic and inflammatory systems during blood feeding. To des...
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PMID: 19900580
PDF is available here.
PDF is available here.
Abstract
The Lesser Mulberry Pyralid, Glyphodes pyloalis, is an important pest of mulberry. This pest feeds on mulberry leaves, and causes some problems for the silk industries in the north of Iran. The study of digestive enzymes is highly imperative to identify and apply new pest management technologies. Gl...
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PMID: 20338537
PDF is available here.
PDF is available here.
Abstract
We also suggest detailed studies on the morphology and function of the salivary glands in order to understand the role of these proteins in host/vector interactions....
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PMID: 20428677
PDF is available here.
PDF is available here.
Abstract
We show that wg and vg are expressed in primordia for tracheal gill and stylus. Those primordia are all located in the lateral body region marked by down-regulation of early segmental wg stripes, but differ in their dorsal-ventral position, indicating their positions drifted within the lateral body...
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PMID: 20433457
PDF is available here.
PDF is available here.
Abstract
We have newly identified three bacteria-induced genes from the silkworm Bombyx mori by quantitative reverse transcriptase-polymerase chain reaction. One of these, eukaryotic initiation factor 4E-1 (eIF4E-1), is assumed to encode an eIF4E family, which plays a role in the initiation of translation as...
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PMID: 20077574
PDF is available here.
PDF is available here.
Abstract
The availability of whole genome sequences of several arthropods has provided new insights into structural cuticular proteins (CPs), in particular the distribution of different families, the recognition that these proteins may comprise almost 2% of the protein coding genes of some species, and the i...
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PMID: 20171281
PDF is available here.
PDF is available here.
Abstract
The silkworm is a model organism for Lepidoptera. Its cuticle is composed mainly of chitin and proteins, which plays essential roles in multiple physiological functions. The binding of proteins to chitin plays an important role for cuticle formation. In this research, a chitin-binding assay followed...
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PMID: 20149871
PDF is available here.
PDF is available here.
Abstract
Querying the genome of the red flour beetle, Tribolium castaneum, with the Drosophila melanogaster Yellow-y (DmY-y) protein sequence identified 14 Yellow homologs. One of these is an ortholog of DmY-y, which is required for cuticle pigmentation (melanization), and another is an ortholog of DmY-f/f2,...
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PMID: 20149870
PDF is available here.
PDF is available here.
Abstract
The exoskeleton of insects (cuticle) is an assembly of chitin and cuticle proteins. Its physical properties are determined largely by the proteins it contains, and vary widely with developmental stages and body regions. The genes encoding cuticle proteins are therefore good models to study the molec...
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PMID: 20060042
PDF is available here.
PDF is available here.