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Polyproteins (3):
Recent article
Polyproteins
Journal of Virology (84)13 2010
Abstract
We demonstrate that exchange of CS1 and/or CS2 with the CS3 p4-p1 amino acids in engineered MHV mutants switches specificity from PLP1 to PLP2 at CS2, but not at CS1, and results in altered protein processing and virus replication. Thus, the p4-p1 residues are necessary for PLP2 processing but requi...
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PMID: 20427532
PDF is available here.
PDF is available here.
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Biophysical Journal (98)8 2010
Abstract
We stretched VWF multimers and a poly-protein (A1A2A3)3 that contains three repeats of the triplet A1A2A3 domains at constant speeds of 2000 nm/s and 400 nm/s, respectively, which yielded corresponding average force loading rates of 90 and 22 pN/s. We found that VWF multimers became stiffer when the...
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PMID: 20409490
PDF is available here.
PDF is available here.
Abstract
We observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data as a form of 'slipstream' translocation: downstream proteins, without signals, were translocated through a translocon pore already formed by the signal...
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PMID: 19946875
PDF is available here.
PDF is available here.
Abstract
We observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data as a form of 'slipstream' translocation: downstream proteins, without signals, were translocated through a translocon pore already formed by the signal...
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PMID: 19946875
PDF is available here.
PDF is available here.
Journal of Virology (84)1 2010
Abstract
We analyzed, by means of an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible virus, the role of polyprotein pp62 in virus assembly. Polyprotein pp62 is indispensable for viral replication. The repression of polyprotein pp62 expression does not alter late gene expression or the proteolytic pro...
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PMID: 19846532
PDF is available here.
PDF is available here.
Journal of General Virology (90)Pt 4 2009
Abstract
We present genomic analyses of a fifth LV strain (64-7855) isolated from a southern red-backed vole (Myodes gapperi) trapped during arbovirus studies in New York state in the north-eastern USA in the 1960s. Sequence analysis of the 64-7855 genome showed an LV-like genome organization and sequence si...
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PMID: 19264646
PDF is available here.
PDF is available here.
Archives of Virology (154)4 2009
Abstract
The full-length genome of a potyvirus, previously known as sunflower chlorotic spot virus isolate SF-1 (SCSV-SF-1) which causes novel symptoms on sunflowers (Helianthus annuus), was sequenced and analyzed. The genome of SCSV-SF-1 is 9,741 nucleotides long, encoding a polyprotein of 3,071 amino acids...
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PMID: 19308314
PDF is available here.
PDF is available here.
Journal of Virology (83)1 2009
Antiviral activity of the zinc ionophores pyrithione and hinokitiol against picornavirus infections.
Abstract
We have discovered two metal ion binding compounds, pyrithione (PT) and hinokitiol (HK), that efficiently inhibit human rhinovirus, coxsackievirus, and mengovirus multiplication. Early stages of virus infection are unaffected by these compounds. However, the cleavage of the cellular eukaryotic trans...
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PMID: 18922875
PDF is available here.
PDF is available here.
Virus Research (137)2 2008
Abstract
We expressed full-length sapovirus genome constructs in insect cells and analyzed their products. The capsid protein was cleaved from the ORF1 polyprotein from a native-like genome construct and two full-length genome constructs with mutations in an active polymerase motif, whereas the capsid protei...
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PMID: 18674574
PDF is available here.
PDF is available here.
Virus Research (136)1-2 2008
Abstract
The complete nucleotide sequence of polyprotein gene 1 and the assembled full-length genome sequence are presented for turkey coronavirus (TCoV) isolates 540 and ATCC. The TCoV polyprotein gene encoded two open reading frames (ORFs), which are translated into two products, pp1a and pp1ab, the latter...
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PMID: 18514348
PDF is available here.
PDF is available here.
Virus Research (135)2 2008
Abstract
The genus Ipomovirus is one of six currently recognized genera in the family Potyviridae. The complete nucleotide sequence of Squash vein yellowing virus (SqVYV), a putative ipomovirus recently described in Florida, has been determined. The 9836 nt SqVYV genomic RNA [excluding the poly(A) tail] has...
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PMID: 18455828
PDF is available here.
PDF is available here.
Journal of Virology (82)15 2008
Abstract
These results implicate nsP2 in regulation of minus-strand synthesis and suggest that different regions of the nsP2 MTase-like domain differentially modulate host defense mechanisms, independent of its role as the viral protease....
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PMID: 18495773
PDF is available here.
PDF is available here.
Abstract
We demonstrate for the first time that ABA triblock protein copolymers when redesigned with substantially larger endblock segments can withstand significantly greater loads. Furthermore, the presence of three distinct phases of deformation behavior was revealed upon subjecting physically cross-linke...
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PMID: 18558738
PDF is available here.
PDF is available here.
Abstract
We have confirmed proteolytic cleavage at the NTB-VPg and VPg-Pro sites. In addition, we have identified two cleavage sites in the N-terminal region of P1. Site-directed mutagenesis and immunoprecipitation experiments using inserted peptide tags confirmed that the position of these cleavage sites co...
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PMID: 18308360
PDF is available here.
PDF is available here.
Virus Research (133)2 2008
Abstract
We constructed a range of gag-derived genes encoding in-frame C-terminal fusions of myristoylation-competent native Pr55Gag and p6-truncated Gag (Pr50Gag) to test the effects of polypeptide length and sequence on VLP formation and morphology, in an insect cell expression system. Fused sequences incl...
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PMID: 18329748
PDF is available here.
PDF is available here.
Abstract
We report the discovery of a short ORF embedded within the P3 cistron of the polyprotein but translated in the +2 reading-frame. The ORF, termed pipo, is conserved and has a strong bioinformatic coding signature throughout the large and diverse Potyviridae family. Mutations that knock out expression...
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PMID: 18408156
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 4 2008
Abstract
Our analyses showed that these sequences fall into two categories. The majority mediated very high (complete) cleavage to separate proteins and a few sequences mediated cleavage with lower efficiency, generating appreciable levels of the uncleaved form. Phylogenetic analyses of 2A-like sequences and...
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PMID: 18343847
PDF is available here.
PDF is available here.
Abstract
We demonstrate a combination of single molecule force spectroscopy and solvent substitution that captures the presence of solvent molecules in the transition state structure. We measure the effect of solvent substitution on the rate of unfolding of the I27 titin module, placed under a constant stret...
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PMID: 18305176
PDF is available here.
PDF is available here.
Abstract
We report the cloning and expression of a 74kDa recombinant polyprotein (Map74F) and its protective efficacy against MAP infection in mice. Map74F was generated by the sequential linkage of the ORFs of the approximately 17.6-kDa C-terminal fragment of Map3527 to the full-length ORF of Map1519, follo...
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PMID: 18243427
PDF is available here.
PDF is available here.
Abstract
We characterized tellina virus 1 (TV-1), a birnavirus isolated from the marine bivalve mollusk Tellina tenuis. Genome sequence analysis established that TV-1 is representative of a viral cluster distant from other birnaviruses. The maturation process of the polyprotein encoded by the genomic segment...
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PMID: 17976679
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 2 2008
Abstract
I (RIG-I) pathway by proteolytically cleaving mitochondria-associated CARD-containing adaptor protein Cardif, and this leads to reduced production of beta interferon (IFN-beta). This study examined the expression of CCL5 (regulated upon activation, normal T-cell expressed and secreted, or RANTES), C...
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PMID: 18198374
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 2 2008
Abstract
The two N-terminal cleavage products, nsp1alpha and nsp1beta, of the replicase polyproteins of porcine reproductive and respiratory syndrome virus (PRRSV) each contain a papain-like autoproteinase domain, which have been named PCPalpha and PCPbeta, respectively. To assess their role in the PRRSV lif...
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PMID: 18198380
PDF is available here.
PDF is available here.
Abstract
We describe the production of transgenic tomato plants that express the structural polyprotein, P1-2A, and protease, 3C, from foot-and-mouth disease (FMDV). P1-2A3C was inserted into the plant binary vector, pBin438, and transformed into tomato plants using Agrobacterium tumefaciens strain, GV3101....
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PMID: 18006078
PDF is available here.
PDF is available here.
Abstract
Abstract
We have used GBV-B infection of tamarins, which develop acute hepatitis following experimental infection, as a surrogate model to study protective immunity. As challenge virus, we first produced a GBV-B pool from an infected tamarin, which was not infected with the related GBV-A viruses. Its infecti...
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PMID: 18041000
PDF is available here.
PDF is available here.
Abstract
As expected, the baculovirus carrying the FK5.1 replicon induced the production of IFN-beta as judged by the use of an IFN-beta promoter luciferase reporter construct, whereas the GND baculovirus (a control polymerase knock-out replicon) and the full-length 3'UTR deletant failed to induce luciferase...
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PMID: 18392092
PDF is available here.
PDF is available here.
Gastroenterology (134)1 2008
Abstract
HCV-induced reactive oxygen species may down-regulate hepcidin transcription through inhibition of C/EBPalpha DNA binding activity by C/EBP homology protein, which in turn leads to increased duodenal iron transport and macrophage iron release, causing hepatic iron accumulation....
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PMID: 18166355
PDF is available here.
PDF is available here.
Archives of Virology (153)10 2008
Abstract
We expressed part of the PSIV polyprotein in Escherichia coli and detected autoprocessed viral proteins. N-terminal sequence analysis of the autoprocessed proteins showed that Q/GWLSW and Q/NGVFD in the PSIV sequence correspond to 2C/3A and 3C/3D cleavage sites in picornaviruses. Alignments of deduc...
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PMID: 18810573
PDF is available here.
PDF is available here.
Archives of Virology (153)7 2008
Abstract
An infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus (PnMV) was generated. It consisted of 6,098 nucleotides and encoded a polyprotein of 219.5 kDa. Sequence comparisons indicated that this isolate shared 98.6% (nucleotide) and 97.1% (amino acid) identity with the previously se...
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PMID: 18504521
PDF is available here.
PDF is available here.
Archives of Virology (153)7 2008
Abstract
We reported experimental verification for a new short overlapping coding sequence (CDS) in the Potyviridae-a family in which overlapping genes were previously unknown. Using the same bioinformatics software (MLOGD), we have identified an approximately 89-codon conserved open reading frame (ORF) with...
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PMID: 18535758
PDF is available here.
PDF is available here.
Abstract
We established conditional expression of the full-length open reading frame (ORF) of an infectious cDNA clone of HCV (genotype 1a, H77 strain) in the nontransformed human hepatocyte line cell HH4 using the ecdysone receptor regulatory system. Treatment with the ecdysone analog ponasterone-A induced...
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PMID: 17991716
PDF is available here.
PDF is available here.
Virus Genes (35)3 2007
Abstract
The complete genomic sequences of Beet mosaic virus Xinjiang (BtMV-XJ) and Inner Mongolia (BtMV-IM) isolates from China were determined and compared with US and German isolates, reported previously. Results showed that viral genome of the two isolates both comprise 9,591 nucleotides, and contain the...
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PMID: 17671838
PDF is available here.
PDF is available here.
Virus Genes (35)3 2007
Abstract
The complete genomic sequence of an isolate of Tobacco vein banding mosaic virus (TVBMV-YND) from Yunnan, China was determined by sequencing overlapping cDNA fragments obtained by RT-PCR with degenerate and/or specific primers. The genome is composed of 9,570 nucleotides (nt) excluding the 3'-termin...
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PMID: 17668310
PDF is available here.
PDF is available here.
Virus Research (130)1-2 2007
Abstract
The N-terminal (NT) regions of particular protein-coding sequences are generally used for in-frame insertion of heterologous open reading frames (ORFs) in potyviral vectors for protein expression in plants. An infectious cDNA clone of Turnip mosaic virus (TuMV) isolate YC5 was engineered at the gene...
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PMID: 17689817
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)47 2007
Molecular determinants of binding between Gly-Leu-Phe-Gly nucleoporins and the nuclear pore complex.
Abstract
We previously proposed that Nup145N, and possibly the other orthologs Nup116 and Nup100, might bind to Nup145C as demonstrated for Nup98 and Nup96. Here we have further investigated the interaction of both yeast and vertebrate Gly-Leu-Phe-Gly nucleoporins with the nuclear pore. We find that dynamic...
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PMID: 17897945
PDF is available here.
PDF is available here.
Abstract
We have previously shown that the putative nucleotide triphosphate-binding protein (NTB) of ToRSV is an ER-targeted protein and that an intermediate polyprotein containing the domains for NTB and for the genome-linked viral protein (VPg) is associated with the replication complex. We now report the...
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PMID: 17658576
PDF is available here.
PDF is available here.
Journal of Virology (81)19 2007
Abstract
We determined the requirement for the nsp7 to nsp10 proteins and their processing during murine hepatitis virus (MHV) replication. Through use of an MHV reverse genetics system, in-frame deletions of the coding sequences for nsp7 to nsp10, or ablation of their flanking Mpro cleavage sites, were made...
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PMID: 17634238
PDF is available here.
PDF is available here.
Infection and Immunity (75)9 2007
Abstract
We demonstrate that Leish-111f is also a vaccine antigen candidate against visceral leishmaniasis (VL) caused by Leishmania infantum. We evaluated the immune response and protection induced by Leish-111f formulated with monophosphoryl lipid A in a stable emulsion (Leish-111f+MPL-SE) and demonstrated...
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PMID: 17606603
PDF is available here.
PDF is available here.
Liver International (27)6 2007
Abstract
Transgenic mice expressing the HCV polyprotein are abnormally vulnerable to oxidative stress. SNMC protects hepatocytes against CCl4-induced oxidative stress and mitochondrial injury in the presence of HCV proteins by restoring depleted cellular GSH....
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PMID: 17617128
PDF is available here.
PDF is available here.
Journal of Virology (81)15 2007
Abstract
We demonstrated that internal cleavages occurred on the NS3 protein of genotype 1b in the presence of NS4A, both in culture cells and with a mouse model system. No internal cleavage products were detected with the NS3 and NS4A proteins of genotype 2a. Three potential cleavage sites were detected in...
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PMID: 17522200
PDF is available here.
PDF is available here.
Journal of Virology (81)13 2007
Abstract
We introduced a single-amino-acid change (nsp10:Q65E) associated with the TS-LA6 phenotype into nsp10 of the infectious clone of MHV. Growth kinetic studies demonstrated that this mutation was sufficient to generate the TS phenotype at permissive and nonpermissive temperatures. Our results demonstra...
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PMID: 17428870
PDF is available here.
PDF is available here.
Abstract
We set out to create an infectious cDNA of the Brazilian prototype strain of DV serotype 1 (DV1-BR/90). PCR-amplified fragments of DV1-BR/90 cDNA were readily assembled into a subgenomic cDNA that could be used to produce replicating RNAs (replicons), lacking the structural protein-encoding regions...
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PMID: 17289102
PDF is available here.
PDF is available here.
Journal of Virology (81)12 2007
Abstract
These results demonstrate that nsp10 is a critical regulator of coronavirus RNA synthesis and may play an important role in polyprotein processing....
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PMID: 17392363
PDF is available here.
PDF is available here.
Journal of Virology (81)11 2007
Abstract
We observed complete autoprocessing at the R and M sites of Marek's disease virus (MDV) protease following production of the polyprotein in Escherichia coli. Site-directed mutagenesis confirmed the predicted sequence of the R and M sites, with the M site sequence being nonconsensual: M(P3)-N(P2)-A(P...
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PMID: 17376905
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)17 2007
Abstract
Flaviviruses are serious human pathogens for which treatments are generally lacking. The proteolytic maturation of the 375-kDa viral polyprotein is one target for antiviral development. The flavivirus serine protease consists of the N-terminal domain of the multifunctional nonstructural protein 3 (N...
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PMID: 17337448
PDF is available here.
PDF is available here.
Journal of Virology (81)8 2007
Abstract
We characterized the proteolytic processing of the human coronavirus 229E (HCoV-229E) amino-proximal pp1a/pp1ab region by two paralogous PLpro activities. Reverse-genetics data revealed that replacement of the PL2pro active-site cysteine was lethal. By contrast, the PL1pro activity proved to be disp...
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PMID: 17251282
PDF is available here.
PDF is available here.
Journal of General Virology (88)Pt 4 2007
Abstract
Previously, it was shown that pyrrolidine dithiocarbamate (PDTC) inhibits proteolytic polyprotein processing and replication of human rhinovirus by transporting metal ions into cells. Here, it is shown that PDTC also inhibits replication of two other picornaviruses: coxsackievirus B3 (CVB3), a close...
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PMID: 17374764
PDF is available here.
PDF is available here.
Biophysical Journal (92)4 2007
Abstract
We use the GCN4 oligomerization domain to engineer a covalently linked parallel polyprotein dimer based on the well-studied I27 domain of titin. We use single molecule atomic force microscopy techniques to stretch single polyprotein fibers and verify their mechanical properties. We find that the eng...
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PMID: 17158577
PDF is available here.
PDF is available here.
Nature Materials (6)2 2007
Abstract
We use single-molecule atomic force microscopy techniques to demonstrate that an artificial polyprotein made of tandem repeats of non-mechanical protein GB1 has mechanical properties that are comparable or superior to those of known elastomeric proteins. In addition to its mechanical stability, we s...
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PMID: 17237787
PDF is available here.
PDF is available here.
Development (134)4 2007
Abstract
We show that one Hts protein, Ovhts, is a polyprotein that is cleaved to produce two products, Ovhts-Fus and Ovhts-RC. Whereas Ovhts-Fus localizes to the fusome in mitotic cells, Ovhts-RC localizes to ring canals throughout later oogenesis. We demonstrate that an uncleavable version of Ovhts delays...
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PMID: 17215303
PDF is available here.
PDF is available here.
Abstract
There is a large demand for new bone regeneration to restore the function during bone injuries. Bone filling materials are important in bone tissue restoration. In this study, the demineralized bone matrix (DBM) was activated with the engineering human bone morphogenetic protein-2 (B...
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PMID: 17013862
PDF is available here.
PDF is available here.
Biophysical Journal (92)1 2007
Abstract
We engineer single disulfide bonds into four different locations of the human cardiac titin module (I27) to control the contour length while keeping the distance to the transition state unchanged. This enables the study of several biologically important parameters. First, we are able to precisely de...
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PMID: 17028145
PDF is available here.
PDF is available here.
Acta Virologica (51)1 2007
Abstract
Porcine teschovirus 1 (PTV-1) (Swine/CH/IMH/03) was isolated from piglets in a farm in Inner Mongolia Province, P.R. China. It was confirmed by electron microscopy, RT-PCR, and sequencing. Comparison of the sequences of the amino acid and nucleotides and phylogenetic analysis of the polyprotein show...
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PMID: 17432938
PDF is available here.
PDF is available here.
Abstract
We have re-examined the wheat germ extract experiments that led to the Trojan horse model, incorporating a variety of controls. We report that (1) translation in vitro from the polyprotein AUG of TYMV RNA is unchanged after removal of the 3' TLS but is stimulated by the presence of a 5'-cap; (2) the...
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PMID: 17095542
PDF is available here.
PDF is available here.
Archives of Virology (152)5 2007
Abstract
A new virus was isolated from tomato plants from the Murcia region in Spain which showed symptoms of 'torrado disease'; very distinct necrotic, almost burn-like symptoms on leaves of infected plants. The virus particles are isometric with a diameter of approximately 28 nm. The viral genome consists...
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PMID: 17226066
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)2 2007
Abstract
The homotrimeric fusion protein nucleocapsid (NC)-dUTPase combines domains that participate in RNA/DNA folding, reverse transcription, and DNA repair in Mason-Pfizer monkey betaretrovirus infected cells. The structural organization of the fusion protein remained obscured by the N- and C-terminal fle...
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PMID: 17169987
PDF is available here.
PDF is available here.
Journal of Virology (81)2 2007
Abstract
We describe here a mathematical model of HCV replication that assumes that the translation of the HCV polyprotein occurs in the cytoplasm, that HCV RNA synthesis occurs in vesicular-membrane structures, and that the strategy of replication involves a double-stranded RNA intermediate. Our results she...
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PMID: 17035310
PDF is available here.
PDF is available here.
Abstract
Fluorescent protein markers are widely used to report plant membrane traffic; however, effective protocols to quantify fluorescence or marker expression are lacking. Here the 20 residue self-cleaving 2A peptide from Foot and Mouth Disease Virus was used to construct polyproteins that expressed a tra...
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PMID: 17118121
PDF is available here.
PDF is available here.
Virus Research (122)1-2 2006
Abstract
We report the first complete genomic sequence of pig bovine viral diarrhea (BVD) virus, that of strain ZM-95, which is 12,220 nucleotides long and contains short 5' and 3' non-coding regions and one open reading frame encoding a large polyprotein with 28 potential N-glycosylation sites (Asn-X-Ser or...
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PMID: 16854490
PDF is available here.
PDF is available here.
Journal of Virology (80)24 2006
Abstract
We have studied the function of protein pB602L during the viral assembly process by using a recombinant ASFV, vB602Li, which inducibly expresses the gene coding for this protein. We show that protein pB602L is a late nonstructural protein, which, in contrast with protein p72, is excluded from the vi...
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PMID: 17035321
PDF is available here.
PDF is available here.