Abstract
We evaluated several open-source analysis tools in terms of performance on two reference data sets, explicitly generated for this purpose. In this paper we present an implementation, T3PQ (Top 3 Protein Quantification), of the method suggested by Silva and colleagues for LC-MS(E) applications and we...
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PMID: 20576481
PDF is available here.
Abstract
These results suggest that enzymatic deproteinization of the shrimp shell wastes, using B. cereus SV1 protease, could be applicable to the chitin production process....
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PMID: 19960271
PDF is available here.
Abstract
The effects of whole milk powder, 2 skim milk powders, caseinate, and 2 modified whey proteins (2% protein level in the final product) were evaluated in lean chicken meat batters and compared with controls with and without added lactose. All dairy proteins significantly (P<0.05) reduced cook losses...
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PMID: 20460680
PDF is available here.
Abstract
Recent studies showed that a combination of carbohydrate and protein was more effective than carbohydrate alone for replenishing muscle glycogen after exercise. However, it remains to be unclear whether the source or degree of hydrolysis of dietary protein influences post-exercise glycogen accumulat...
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PMID: 19593593
PDF is available here.
Abstract
Our results suggested that the commercial microbial proteases such as B. subtilis and B. licheniformis could be used to produce effective antioxidant hydrolysates from food proteins.
Copyright 2009 Elsevier Ltd. All rights reserved....
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PMID: 20015640
PDF is available here.
Abstract
We conducted a randomized, placebo-controlled, double blind, crossover study including 70 Caucasian subjects with prehypertension or stage 1 hypertension. Study treatments consisted of daily consumption of two capsules MPH1 (each containing 7.5 mg Isoleucine-Proline-Proline; IPP), MPH2 (each contain...
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PMID: 21059213
PDF is available here.
Abstract
Degree of hydrolysis (DH) is defined as the proportion of cleaved peptide bonds in a protein hydrolysate. Several methods exist for determining DH; the most commonly used of these include the pH-stat, trinitrobenzenesulfonic acid (TNBS), o-phthaldialdehyde (OPA), trichloroacetic acid soluble nitroge...
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PMID: 21140664
PDF is available here.
Abstract
Effects of chicken collagen hydrolysate (CCH) on atherogenesis in apolipoprotein E-deficient C57BL/6.KOR-ApoE(shl) mice were investigated in this paper. The mice were fed on either a normal CE-2 diet (control group) or a diet containing 10% CCH (CCH group) for 12 wk. Compared with that of the contro...
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PMID: 20651463
PDF is available here.
Abstract
The total effective rate of 91.4% in the acupoint injection group was superior to that of 73.7% in the medication group (P<0.05), the acupoint injection group could significantly improve the systolic peak velocity (Vs), end diastolic velocity (Ved) and mean velocity (Vm) of middle cerebral artery (M...
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PMID: 19873914
PDF is available here.
Abstract
In this study, the repeated-batch fermentation of liquefied cassava medium using the flocculent hybrid Saccharomyces cerevisiae CHFY0321 was investigated for semicontinuous, high-throughput production of bioethanol. Cassava medium was selected due to the industrial requirement for a cheap starchy su...
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PMID: 19319524
PDF is available here.
Abstract
We show that rats fed a diet containing saithe fish protein hydrolysate (saithe FPH), rich in taurine and glycine, for 26 days had markedly elevated fasting plasma BA levels relative to rats fed soy protein or casein. Concomitantly, the saithe FPH fed rats had reduced liver lipids and fasting plasma...
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PMID: 19416649
PDF is available here.
Abstract
Chicken collagen hydrolysate was given to 15 mildly hypertensive subjects for 4 weeks. Blood pressure was significantly decreased by 11.8 mmHg (P<0.01). A reduction in plasma renin activity was observed in blood test after intake. A colony assay of endothelial progenitor cells in blood samples from...
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PMID: 19202283
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Abstract
We confirmed previous observations indicating that McPhail-type traps are quite inefficient. Considering the total 24-h fly tenure in the cage, and independent of bait treatment and fly type (i.e., strain, adult diet, gender and age), of a total of 2,880 A. obliqua and 2,880 A. ludens adults release...
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PMID: 19253622
PDF is available here.
Abstract
The efficiency of enzymatic hydrolysis and leaching with water using accelerated solvent extraction (ASE) or boiling was investigated for quantitative Se speciation in selenized potatoes using reversed phase HPLC coupled to ICP-MS. Preliminary identification of selenomethionine (SeMet), Se-methylsel...
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PMID: 19093878
PDF is available here.
Abstract
The body weight of initially HA-fed children was 7870G (SD 949) significantly higher as the one of breastfed children (7508 G, SD 912, p=0.0571), in addition the weight increase was also significantly higher in HA-fed infants at the age of 6 months (p=0.0042). The frequency of AD as well as SCORAD s...
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PMID: 18270883
PDF is available here.
Abstract
The aim of this study was to use the hydrolysate of pig spinal cord proteins to induce oral tolerance in the animal model of sclerosis multiplex - experimental allergic encephalomyelitis. The female Lewis rats were fed with hydrolysate of pig spinal cord proteins in two doses for one week before imm...
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PMID: 19325643
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Abstract
In the review a number of fermental preparations and the food substrata used for reception hydrolyzates of proteins included in them, and also some questions of embedding of transitive metals in protein and peptide matrixes with formation of organic forms essential trace elements is characterized. C...
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PMID: 20387693
PDF is available here.
Abstract
Rice bran, an economical, underutilized coproduct of rough rice milling, was used to produce peptide hydrolysates, which were investigated for anticancer activity. Protein hydrolysates prepared by Alcalase hydrolysis under optimized conditions were treated further to obtain gastrointestinal (GI)-res...
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PMID: 19090710
PDF is available here.
Abstract
Proteases from pyloric caeca extract of three fish species including brownstripe red snapper (Lutjanus vitta), bigeye snapper (Priacanthus tayenus) and threadfin bream (Nemipterus marginatus) were comparatively studied. The extracts from bigeye snapper and threadfin bream exhibited the highest hydro...
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PMID: 18793744
PDF is available here.
Abstract
We further investigated its antioxidant activities on cellular systems, and the results showed that APTDM significantly scavenged cellular radicals and enhanced the viability of tert-butyl hydroperoxide-induced cytotoxicity. These results indicate that APTDM or a peptide fraction containing APTDM wo...
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PMID: 19053853
PDF is available here.
Abstract
We have investigated alkali-solubilized tilapia ( Oreochromis niloticus) protein hydrolysates for their ability to scavenge reactive oxygen species (ROS) and for their reducing power. Tilapia protein isolate was prepared by an alkaline solubilization technique and used as a substrate for enzyme hydr...
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PMID: 18828605
PDF is available here.
Abstract
Studies on enzymatic hydrolysis of cell proteins in green microalgae Chlorella vulgaris 87/1 are described. Different proteases can be used for production of hydrolysates from ethanol extracted algae. The influence of reaction parameters on hydrolysis of extracted biomass with pancreatin was conside...
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PMID: 18359627
PDF is available here.
Abstract
Identification of labeled peptides has shown that the probe (binding as a substrate to the enzyme) was attached to fragments: 30-48 (the most likely positions of the label are Leu44, Gln45 and Asp47), 127-140 (with Arg133 labeled, as indicated by mass spectrometry), 359-373 and 434-443 (the exact po...
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PMID: 18987581
PDF is available here.
Abstract
These results suggest that the commercial collagen hydrolysates of bovine and porcine origin may be a potential source of bioactive peptides....
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PMID: 18800907
PDF is available here.
Abstract
Microfluidic devices were used to study the influences of hydrodynamics of local microenvironments on Staphylococcus epidermidis (S. epidermidis) biofilm formation and the effects of a poly(beta-1,6-N-acetyl glucosamine)-hydrolyzing enzyme (dispersin B) and/or an antibiotic (rifampicin) on the detac...
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PMID: 18204904
PDF is available here.
Abstract
We concluded that fraction 3 had the most potential to be used as a bioactive ingredient because of its strong ACE-inhibitory and free-radical scavenging properties....
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PMID: 18636366
PDF is available here.
Abstract
We have examined whether peptides derived from food proteins might influence bile acid synthesis. A reporter gene cell line that carries a cholesterol 7alpha-hydroxylase promoter fragment fused to firefly luciferase ( cyp7a-luc) was used to screen for nutritive peptides affecting cyp7a expression, t...
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PMID: 18543926
PDF is available here.
Abstract
Protein hydrolysate was prepared from visceral waste proteins of an Indian freshwater major carp, Catla catla. Hydrolysis conditions (viz., time, temperature and enzyme to substrate level) for preparing protein hydrolysates from the fish visceral waste proteins using in situ pH of the visceral mass...
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PMID: 17933524
PDF is available here.
Abstract
We present a new pI calculation algorithm that takes into account the effect of adjacent amino acids on the pI value. The algorithm accounts for the effect of adjacent amino acids+/-3 residues away from a charged aspartic or glutamic acid, as well as effects on the free C terminus, and applies a cor...
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PMID: 18615785
PDF is available here.
Abstract
In-channel atom transfer radical polymerization (ATRP) was used to graft a PEG layer on the surface of microchannels formed in poly(glycidyl methacrylate)-co-(methyl methacrylate) (PGMAMMA) microfluidic devices. The patterned and cover plates were first anchored with ATRP initiator and then thermall...
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PMID: 18615784
PDF is available here.
Abstract
We compared the tolerance and growth of infants with CMPA who were fed a new extensively hydrolyzed formula containing lactose (eHF) with those who were fed an amino acid formula (AAF). This was a prospective, multi-center, randomized, reference-controlled study. Seventy-seven infants <12 months old...
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PMID: 18167160
PDF is available here.
Andrea von Berg,
Birgit Filipiak-Pittroff,
Ursula Krämer,
Elke Link,
Christina Bollrath,
Inken Brockow,
Sibylle Koletzko,
Armin Grübl,
Joachim Heinrich,
H-Erich Wichmann,
Carl-P Bauer,
Dietrich Reinhardt,
Dietrich Berdel and
GINIplus study group
Abstract
In the intent-to-treat analysis the relative risk of a physician's diagnosis of allergic manifestation (AM) compared with CMF was 0.82 (95% CI, 0.70-0.96) for partially hydrolyzed whey formula, 0.90 (95% CI, 0.78-1.04) for extensively hydrolyzed whey formula, and 0.80 (95% CI, 0.69-0.93) for extensi...
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PMID: 18539195
PDF is available here.
Abstract
The cumulative percentage of tolerance to CM at 6 and 12 months was similar in both groups: 56 (77%) in the probiotics group versus 54 (81%) in the placebo group. Infants in the placebo group had higher percentages of CD3(+) and CD3(+)CD4(+) lymphocytes compared with those seen in probiotic-treated...
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PMID: 18436293
PDF is available here.
Abstract
We investigated the effects of soy protein and soy protein hydrolysate on blood pressure control, angiotensin-converting enzyme (ACE) activity, and renal function in a rat chronic renal failure model. Rats that had undergone a 5/6 nephrectomy were separated into three groups and fed different experi...
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PMID: 18712051
PDF is available here.
Abstract
When data from all subjects (n = 97) were evaluated, six parameters showed statistically significant changes with the dietary supplement collagen hydrolysate (CH) compared with placebo: joint pain at rest, assessed by the physician (CH vs. placebo (-1.37 +/- 1.78 vs. -0.90 +/- 1.74 (p = 0.025)) and...
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PMID: 18416885
PDF is available here.
Abstract
In the present study, the changeover from the Pico.Tag HPLC method to the AccQ.Tag(ultra) UPLC method for the analysis of amino acids in casein and bovine serum albumine hydrolysates is described. The total chromatographic run time of the AccQ.Tag(ultra) UPLC method was only 40% of the time required...
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PMID: 18070624
PDF is available here.
Abstract
The objective of the study was to assess the antioxidant potential of alcalase-treated zein hydrolysate (ZH) during a two-stage (1 h of pepsin --> 0.5-2 h of pancreatin, 37 degrees C) in vitro digestion. Sephadex gel filtration and high-performance size exclusion chromatography were used to separate...
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PMID: 18376842
PDF is available here.
Abstract
I band underwent a shift to lower wave numbers. A wide, intense Ca-O absorption band also appeared between 400 and 100 cm(-1) in the far-infrared spectrum. The width and intensity of this band increased after treatment of samples with glutaminase. The amount of bound calcium was related to both the...
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PMID: 18387086
PDF is available here.
Abstract
Canola is an economically important farm-gate crop in Canada. To further explore the potential of canola protein as value-added food and nutraceutical ingredients, a better understanding of fundamental properties of 2 major canola proteins is necessary. Two major protein components, cruciferin and n...
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PMID: 18387101
PDF is available here.
Abstract
I (SH I) or soy hydrolysate II (SH II) three times weekly for 2 weeks. Krebs solution and leptin were used as negative and positive controls respectively. SH I (6.5-20 kDa with a strong band at 14 kDa) was produced by hydrolysis with alcalase, and SH II (approximately 2 kDa) was obtained by hydrolys...
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PMID: 18157697
PDF is available here.
Abstract
Some indications of lower hunger (experiment 1), desire to eat (experiment 2) and thirst (experiment 1 and 2) were shown after consumption of PPH compared to MP or WP+PPH (P<0.05). A longer intermeal interval and a higher satiety index were suggested after consumption of PPH. Both PPH and WP lead to...
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PMID: 18345020
PDF is available here.
Abstract
The antioxidant activities of alkali-treated tilapia protein hydrolysates were determined by their ability to inhibit the formation of lipid hydroperoxides (PV) and thiobarbituric acid reactive substances (TBARS) in a washed muscle model system and by their ability to inhibit DPPH free radicals and...
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PMID: 18247531
PDF is available here.
Abstract
We set up an in vitro assay system to investigate if different protein hydrolysates exhibit varying CCK-releasing properties. Soy, pea, potato, casein, and whey protein hydrolysates were incubated with the enteric endocrine cell line STC-1 that endogenously expresses and secretes CCK. Release of CCK...
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PMID: 18211011
PDF is available here.
Abstract
Effects of the enzymes in Actinomucor elegans extract and the enzyme Alcalase 2.4L on debittering the soybean protein hydrolysates were investigated. When the protein was treated only with the latter, a strong bitterness formed; but it decreased if the protein was treated with both the enzymes. The...
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PMID: 17943333
PDF is available here.
Abstract
Quality of proteins depends on their composition in essential amino acids and on the availability of amino acids. Great interest has been shown in the role played by hydrolysates of proteins in clinical diets for pathologies with reduced absorptive capacity and food allergies caused by intact protei...
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PMID: 18727562
PDF is available here.
Abstract
In order to get products with antioxidant activity from Arca subcrenata Lischke, the optimal hydrolase and hydrolysis conditions were investigated in the paper. Three proteases (neutrase, alcalase and papain) were applied to hydrolyze the homogenate of A. subcrenata. An orthogonal design was used to...
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PMID: 19172198
PDF is available here.
Abstract
We have found that traces of fresh substrates at microg g(-1) soil concentrations (termed 'trigger molecules') activate the biomass to expend more energy than is contained in the original 'trigger molecules'. In contrast, we suggest that the rate limiting step in soil organic matter mineralisation i...
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PMID: 18383584
PDF is available here.
Abstract
Enzymatic hydrolysates of waste collagen proteins (H), from current industrial manufacture (leather, edible meat product casings, etc.) of mean molecular mass 20-30 kDa by a reaction with dialdehyde starch (DAS), produces hydrogels applicable as biodegradable (or even edible) packaging materials for...
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PMID: 17376664
PDF is available here.
Abstract
We determined that the antimicrobial effects observed on BHI plates were due to the generation of H2O2 by xanthine oxidase, a major protein constituent of the MFGMs, as a result of purine catalysis. This substrate is present in BHI but lacking in LB medium. Evaluation of purified xanthine oxidase al...
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PMID: 18236672
PDF is available here.
Abstract
We report a case of a preterm newborn with fetal peritonitis, jejunal atresia with volvulus of post-atresic small bowel since about 30 cm from ileo-cecal valve (ICV) followed by a not-used microileus and microcolon. The surgical intervention consisted in the resection of volvulated necrotic small bo...
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PMID: 18668397
PDF is available here.
Abstract
These results suggest that TDA could be applied for the alternative of bitterness evaluation to the hedonic scale sensory evaluation....
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PMID: 18211368
PDF is available here.
Abstract
The effect of the acid and the osmotic stress on the heat resistance of Escherichia coli (EC1 and EC2) was studied at 63 degrees C in tryptic soy broth adjusted to various pHs (2.5, 4.5 and 6) and various NaCl concentrations (2, 4 and 8%). In the second study, the effect of pretreatment on thermotol...
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PMID: 18088012
PDF is available here.
Abstract
1. The effects of 2% whole milk proteins, milk protein's two major components (caseinate and whey) and some of their fractions (beta-lactoglobulin and two hydrolysed whey proteins) were evaluated in a frankfurter-type product made from mechanically deboned chicken meat. 2. Adding caseinate, regular...
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PMID: 18085447
PDF is available here.
Abstract
Twelve kinds of marine protein materials, including fish, shrimp, seashell, algae and seafood wastes were selected for the hydrolysis using four different proteases. The IC(50) values for angiotensin-converting enzyme (ACE) inhibitory activity of 48 hydrolysates were rapidly determined by capillary...
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PMID: 17317156
PDF is available here.
Abstract
Protein hydrolysate formulas for preterm infants recently have been developed. However, the indications for the use of such formulas are unclear. The aim of this article is to systematically evaluate the effect of protein hydrolysate formulas in the feeding of preterm infants through searching the M...
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PMID: 18185089
PDF is available here.