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Protein Isoforms (1):
Recent article
Protein Isoforms
Development (138)21 2011
Abstract
We demonstrate that zebrafish Neuregulin 1 (Nrg1) type III, which signals through ErbB receptors, controls Schwann cell migration in addition to its previously known roles in proliferation and myelination. Chimera analyses indicate that ErbB receptors are required in all migrating Schwann cells, and...
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PMID: 21965611
PDF is available here.
PDF is available here.
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Abstract
We show that MyBP-C slow comprises a family of phosphoproteins. Ser-59 and Ser-62 are substrates for PKA, while Ser-83 and Thr-84 are substrates for PKC. Moreover, Ser-204 is substrate for both PKA and PKC. Importantly, the levels of phosphorylated skeletal MyBP-C proteins (i.e. slow and fast) are n...
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PMID: 21888435
PDF is available here.
PDF is available here.
FEBS Journal (278)19 2011
Abstract
We investigated, for the first time, the alterations of the bovine milk N-glycome during early lactation (1 day, 1, 2, 3 and 4 weeks postpartum), characterizing more than 80 N-glycans. The glycomic profile of colostrum on day 1 after calving differed substantially from that in other periods during e...
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PMID: 21834831
PDF is available here.
PDF is available here.
Molecular Cell (43)6 2011
Abstract
We describe 31 dominant-negative, secreted variant isoforms of receptor tyrosine kinases (RTKs) that are produced by activation of intronic poly(A) sites. We show that blocking U1-snRNP can activate IPA, indicating a larger role for U1-snRNP in RNA surveillance. Moreover, we report the development o...
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PMID: 21925381
PDF is available here.
PDF is available here.
FEBS Journal (278)18 2011
Abstract
We performed MS to identify excretory-secretory proteins from Strongyloides ratti. In the excretory-secretory proteins of the parasitic female stage, we detected, in addition to other peptides, peptides homologous with the Caenorhabditis elegans heat shock protein (HSP)-17, named Sra-HSP-17.1 (∼ 1...
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PMID: 21762402
PDF is available here.
PDF is available here.
Abstract
We also observed that, similarly to SRIF, the specific knockdown of CaMKIIβ induces a decrease of both GH content/secretion. These results raise the hypothesis that CaMKIIβ may mediate, at least in part, the SRIF-induced control of [Ca(2+)](i). In addition, CaMKIIβ seems to play a positive role i...
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PMID: 21952434
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
We explore the stromal proteome of spinach and Arabidopsis chloroplast and show that clustering of mass spectra is a useful tool for investigating such variants and detecting modified peptides with amino acid substitutions or post-translational modifications. This study employs data mining by hierar...
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PMID: 21539947
PDF is available here.
PDF is available here.
Journal of Cell Science (124)Pt 16 2011
Abstract
We provide novel insights into the roles of the CB2 splice variants, CB2(SH3+) and CB2(SH3-), and their substrate, Cdc42, in regulating gephyrin clustering at GABAergic synapses. Transfection of Myc-tagged CB2(SH3+) and CB2(SH3-) into cultured neurons revealed strong, but distinct, effects promoting...
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PMID: 21807943
PDF is available here.
PDF is available here.
Abstract
We have shown that snakes co-opt a non-heat-sensitive channel, vertebrate TRPA1 (transient receptor potential cation channel A1), to produce an infrared detector. Here we show that vampire bats tune a channel that is already heat-sensitive, TRPV1, by lowering its thermal activation threshold to abou...
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PMID: 21814281
PDF is available here.
PDF is available here.
Abstract
We conclude the following: i) overexpression of ΔNp63 contributes to salivary tumorigenesis, ii) ΔNp63 plays a dominant negative effect on the TA isoform in the modulation of cell migration and invasion, and iii) the ΔN isoform plays an oncogenic role and may represent an attractive target for th...
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PMID: 21703418
PDF is available here.
PDF is available here.
Abstract
We have developed tools for IHC of RREB1 protein isoform-specific amplification of RREB1 mRNA and selective knockdown of RREB1 isoforms and use these to provide new information by characterizing RREB1 expression in bladder and prostate cancer cell lines and human tissue samples. Previously described...
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PMID: 21703425
PDF is available here.
PDF is available here.
Abstract
Enzymes acetylcholinesterase (AChE; E.C. 3.1.1.7) and butyrylcholinesterase (BChE; E.C. 3.1.1.8) have intensively been investigated in biomedicine and toxicology due to important role in organisms. Even if structurally homologous, they differ in catalytic activity, specificity, for s...
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PMID: 21705306
PDF is available here.
PDF is available here.
Abstract
We show that AFCs express pluripotency markers (Nanog, Sox2, and Oct3/4) as well as retinal transcription factor genes (Et, Lhx2, Tll1, Six6, Otx2, Pax6, and Fgf15). AFCs from amniotic fluid of Fgf15.gfp, Nrl.gfp, and Crx.gfp embryos cultured in retinal proliferation and differentiation conditions f...
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PMID: 20939691
PDF is available here.
PDF is available here.
Poultry Science (90)5 2011
Abstract
We cloned the gene of goose PRLR (gPRLR) in the Siji goose, a domestic goose with strong broodiness in China, and examined its expression level in different organs of adult geese. Our results showed that gPRLR cDNA contained 443 bp 5' untranslated region, 2,496 bp coding sequence that presumably com...
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PMID: 21489954
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)9 2011
Abstract
We examined these and other critical residues by site-directed mutagenesis substituting Asn227→Ala, Asp, Arg; Ile233→Ala; Leu243→Ala; Glu247→Asp, Gln; Glu248→Asp, Gln. Mutant NHE1 proteins were characterized in AP-1 cells, which do not express endogenous NHE1. All the TMVI critical amino a...
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PMID: 21600870
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)9 2011
Abstract
We previously reported the existence of different TMEM16A isoforms generated by alternative splicing. In the present study, we have determined the functional properties of a minimal TMEM16A protein. This isoform, called TMEM16A(0), has a significantly shortened amino-terminus and lacks three alterna...
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PMID: 21645494
PDF is available here.
PDF is available here.
Current Eye Research (36)4 2011
Abstract
We examined mouse and rat lenses to determine if alternative splicing of neuronal nPTB and Fox-1/Fox-2 isoforms also occurs in lenses.
Immunoblot, immunofluorescence, and RT-PCR were used to examine expression and alternative splicing of transcripts in lens and brain....
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PMID: 21714144
PDF is available here.
PDF is available here.
Abstract
Calcineurin is an important signal transduction mediator in T cells, neurons, the heart, and kidneys. Recent evidence points to unique actions of the two main isoforms of the catalytic subunit. Although the β isoform is required for T-cell development, α is important in the brain a...
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PMID: 21435446
PDF is available here.
PDF is available here.
Abstract
To investigate three isoforms of survivin in colorectal adenocarcinomas.
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PMID: 21472129
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1816)1 2011
Abstract
Signal transducers and activators of transcription (STATs) are, as the name indicates, both signal transducers and transcription factors. STATs are activated by cytokines and some growth factors and thus control important biological processes. These include cell growth, cell differentiation, apoptosi...
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PMID: 21447371
PDF is available here.
PDF is available here.
Abstract
We shall evaluate the roles of 10 functional isoforms of MT in breast neoplasia and discuss the utility of MT isoforms as biomarkers for prognosis. Strategies targeting MT for treatment could provide alternatives to overcome this dreaded disease which has claimed the lives of many women....
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PMID: 21449882
PDF is available here.
PDF is available here.
Abstract
We report several proteins and peptides that exhibit heightened degradation sensitivity, for instance superoxide dismutase in liver, and peptidyl-prolyl cis-trans isomerase and insulin C-peptides in pancreas. Tissue sampling based on snap freezing produces a greater amount of degradation products an...
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PMID: 20110281
PDF is available here.
PDF is available here.
Abstract
We hypothesized that ranolazine will similarly inhibit the paramyotonia congenita NaV 1.4 gain-of-function mutations, R1448C, R1448H, and R1448P that are associated with repetitive action potential firing. Whole-cell Na (+) current (INa) was recorded from HEK293 cells expressing the hNaV 1.4 WT or R...
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PMID: 21317558
PDF is available here.
PDF is available here.
Abstract
We show that a short 15-60 min predigestion with a protease such as LysC (modified on-bead digest termed protease elution) increases the elution efficiency 2- to 3-fold compared to standard acid elution protocols. While longer incubation periods-as performed in standard on-bead digestion-led to part...
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PMID: 21043528
PDF is available here.
PDF is available here.
Abstract
Abstract
We used a mouse myoblast cell line (C2C12) as an in vitro model to investigate the role of PKDs, in particular PKD2, in muscle stem cell differentiation. We show that C2C12 cells express all PKD isoforms with PKD2 being highly expressed. Furthermore, we demonstrate that PKD2 is specifically phosphor...
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PMID: 21298052
PDF is available here.
PDF is available here.
Abstract
We describe the genetic background of full-length ETV1 overexpression and the biological properties of different full-length ETV1 isoforms in prostate cancer. Break-apart FISH showed in five out of six patient samples with overexpression of full-length ETV1 a genomic rearrangement of the gene, indic...
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PMID: 21298110
PDF is available here.
PDF is available here.
Abstract
Akt/PKB is a promising anticancer therapeutic target, since abnormally elevated Akt activity is directly correlated to tumor development, progression, poor prognosis and resistance to cancer therapies. Currently, the unique role of each Akt isoform and their relevance to human breast cancer are poorl...
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PMID: 21297943
PDF is available here.
PDF is available here.
Abstract
We investigated whether USP7 itself regulates PML-NBs. Silencing of USP7 was found to increase the number of PML-NBs, to increase the levels of PML protein and to inhibit PML polyubiquitylation in nasopharyngeal carcinoma cells. This effect of USP7 was independent of p53 as PML loss was observed in...
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PMID: 21305000
PDF is available here.
PDF is available here.
Abstract
We expressed its actin-binding domain (ABD) in CA1 pyramidal neurons in hippocampal slice cultures. The ABD of βI-spectrin bundled actin in principal dendrites and was concentrated in dendritic spines, where it significantly increased the size of the spine head. These effects were not observed afte...
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PMID: 21297961
PDF is available here.
PDF is available here.
Abstract
Progesterone (P) participates in the regulation of several reproductive processes such as ovulation and sexual behavior, however, this hormone also participates in non-reproductive processes, such as neural excitability, learning and memory, and pathological processes such as cancer....
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PMID: 21463255
PDF is available here.
PDF is available here.
Clinical Science (120)2 2011
Abstract
The sRAGE [soluble RAGE (receptor for advanced glycation end-products)] lack the transmembrane and cytoplasmic domain of the full-length receptor and can function as a decoy for RAGE ligands. Recent evidence suggests that sRAGE may be a potential biomarker of RAGE-mediated pathology. The present stu...
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PMID: 20726839
PDF is available here.
PDF is available here.
Abstract
We investigated adiponectin isoforms in nonalcoholic fatty liver disease in relation to liver disease severity and insulin resistance.
Total adiponectin and isoform distribution were measured in 54 biopsy-proven, non-diabetic nonalcoholic fatty liver disease subjects, divided accordi...
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PMID: 20554486
PDF is available here.
PDF is available here.
Abstract
Progesterone (P4), which is produced by the corpus luteum (CL), creates proper conditions for the embryo implantation, its development, and ensures proper conditions for the duration of pregnancy. Besides the non-genomic activity of P4 on target cells, its main physiological effect is caused through...
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PMID: 21528728
PDF is available here.
PDF is available here.
BMC Plant Biology (11) 2011
Abstract
We have identified and annotated the SERK genes in this species. Using degenerate PCR and database mining, eight more SERK-like genes have been identified and these have been shown to be expressed. The amplification and sequencing of several different PCR products from one of these genes is consiste...
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PMID: 21385462
PDF is available here.
PDF is available here.
Abstract
We examined CCL23 production and release by human eosinophils in vitro.
Using Ficoll and antibody-coated immunomagnetic beads, eosinophils and other blood cells were purified from peripheral blood samples obtained from normal subjects and mildly allergic patients. Eosinophils were cu...
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PMID: 21646793
PDF is available here.
PDF is available here.
Abstract
In this study, phorbol-12-myristate-13-acetate (PMA) at low concentrations (<10 nM; L-PMA) induces the differentiation of CD14(+) monocytes into monocyte-derived macrophages (MDMs) while PMA at high concentrations (>100 nM; H-PMA) causes the apoptosis of these cells. The pre-treatment with Go6976 (a...
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PMID: 20626007
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)3 2011
Abstract
We show that H4IIE liver cells express all three types of IP₃ receptor, but only type 1 and to a lesser extent type 3, but not type 2, participate in the activation of CRAC current (I(CRAC)), while type 1 and type 2, but not type 3, participate in observed Ca²+ release in response to receptor sti...
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PMID: 21182823
PDF is available here.
PDF is available here.
Abstract
A three dimensional model was developed for Cry10Aa protein sequence of B. thuringiensis LDC-9 and B. thuringiensis israelensis that has not been solved empirically by X-ray crystallography or NMR. Homology modeling was employed for the structure prediction using Cry2Aa as template protein, a high-r...
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PMID: 20919752
PDF is available here.
PDF is available here.
Abstract
We examined the effects of hypoxia (1% O(2)) and VEGF on the expression of AnxA2 in osteoblastic MC3T3-E1 cells. Hypoxia, desferrioxamine (hypoxia mimetic), and recombinant VEGF all increased AnxA2 mRNA and protein levels in osteoblastic cells. The hypoxia-induced increase in AnxA2 was inhibited by...
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PMID: 20817051
PDF is available here.
PDF is available here.
Abstract
The unicellular green alga Chlamydomonas reinhardtii has at least six plant-type ferredoxins (FDX). Besides the long-known photosynthetic ferredoxin PetF the isoforms Fdx2-Fdx6 have been identified. The FDX genes are differentially expressed under various environmental conditions suc...
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PMID: 20696493
PDF is available here.
PDF is available here.
Molecular Cell (40)3 2010
Abstract
We have discovered an E3 ubiquitin ligase, called Psh1, which controls the cellular level of Cse4 via ubiquitylation and proteolysis. The activity of Psh1 is dependent on both its RING and zinc finger domains. We demonstrate the specificity of the ubiquitylation activity of Psh1 toward Cse4 in vitro...
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PMID: 21070970
PDF is available here.
PDF is available here.
Laboratory Investigation (90)11 2010
Abstract
We searched for the most suitable isoform of the 14-3-3 protein to test for in protein assays, and the most sensitive antibody among four antibodies with an affinity for 14-3-3. We measured the levels of all 14-3-3 isoforms in 112 patients with CJD and in 100 patients with other diseases. We compare...
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PMID: 20697380
PDF is available here.
PDF is available here.
Journal of Trauma (69)5 2010
Abstract
The aim of this study was to investigate the protein kinase C (PKC) isoforms involved in the regulation of vascular calcium sensitivity after hemorrhagic shock, the related mechanism, and the role of integrin-linked kinase (ILK).
Using superior mesenteric artery from hemorrhagic shock rats and hypox...
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PMID: 20495491
PDF is available here.
PDF is available here.
Current protocols in protein science / editorial board, John E. Coligan ... [et al.] (Chapter 14) 2010
Abstract
I (GGTase I), and Rab geranylgeranyl transferase (also termed geranylgeranyltransferase type II - GGTase II). In this unit, synthetic isoprenoids conjugated to either a fluorophore or biotin group are used to assay the activity of protein prenyltransferases in vitro or to affinity tag prenylatable p...
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PMID: 21104984
PDF is available here.
PDF is available here.
Biochemical Journal (432)1 2010
Abstract
We investigated the relationship between Fbw7 and PKC. Multiple members of the PKC superfamily interact with the substrate-binding domain of Fbw7. However, we find no evidence for Fbw7-mediated degradation of PKC. Instead, we demonstrate that Fbw7 is a novel substrate for PKC. Two residues within th...
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PMID: 20815813
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)43 2010
Abstract
We examine the effect of the β3-subunit on the gating behavior of Na(v)1.3 using whole cell patch clamp electrophysiology in HEK-293 cells. We show that β3 depolarizes the voltage sensitivity of Na(v)1.3 activation and inactivation and induces biphasic components of the inactivation curve. We dete...
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PMID: 20675377
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)43 2010
Abstract
We show a novel actin-nucleating activity in mammalian Tmods. Comparison of Tmod isoforms revealed that Tmod1-3 but not Tmod4 nucleate actin filament assembly. All Tmods bind to monomeric actin, and Tmod3 forms a 1:1 complex with actin. By truncation and mutagenesis studies, we demonstrated that the...
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PMID: 20650902
PDF is available here.
PDF is available here.
Abstract
K(v)11.1 channels are involved in regulating cellular excitability in various tissues including brain, heart and smooth muscle. In these tissues, at least two isoforms, K(v)11.1a and K(v)11.1b, with different kinetics, are expressed. K(v)11.1 activators are potential therapeutic agents, but their ef...
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PMID: 20880400
PDF is available here.
PDF is available here.
Laboratory Investigation (90)10 2010
Abstract
We investigated the molecular mechanisms responsible for ErbB4-induced cell survival. In cultured mouse colon epithelial cells, ErbB4 overexpression resulted in increased levels of cyclooxygenase-2 (COX-2) mRNA and protein; in contrast, ErbB4 knockdown with siRNA blocked COX-2 accumulation in respon...
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PMID: 20585313
PDF is available here.
PDF is available here.
Abstract
We hypothesize that OPN isoforms play different roles in determining the metastatic potential of NSCLC.
We amplified mRNA for each OPN isoform in NSCLC tumors and matched normal lung. The functional impact of each isoform was evaluated by transfecting cDNA plasmids specific to each isoform into NSCL...
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PMID: 20689445
PDF is available here.
PDF is available here.
Nature Methods (7)10 2010
Abstract
We developed a method to analyze massively parallel RNA sequence data to catalog transcripts and assess differential and alternative expression of known and predicted mRNA isoforms in cells and tissues. As proof of principle, we used the approach to compare fluorouracil-resistant and -nonresistant h...
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PMID: 20835245
PDF is available here.
PDF is available here.
Abstract
We evaluated the possible routes of the β-CN (193-209) peptide transport, using selective inhibitors of the different routes for peptide transfer through the intestinal barrier. The results showed that the β-CN (193-209) peptide resisted the action of brush-border membrane peptidases, and that it...
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PMID: 20397193
PDF is available here.
PDF is available here.
Abstract
We treated animals with the microtubule depolarizing agent colchicine. Two days of 0.4 mg/kg/day intraperitoneal colchicine blocked autophagosome maturation to autolysosomes and increased LC3-II protein levels in mouse skeletal muscle by >100%. The addition of an autophagic stimulus such as dietary...
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PMID: 20657169
PDF is available here.
PDF is available here.
B M B Reports (43)10 2010
Abstract
We analyzed the effect of APP-swe in global gene expression using deep transcriptome sequencing technique. We found 283 genes were down-regulated and 348 genes were up-regulated in APP-swe expressing H4-swe cells compared to H4 wild-type cells from a total of approximately 74 million reads of 38 bas...
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PMID: 21034535
PDF is available here.
PDF is available here.
Histology and histopathology (25)10 2010
Abstract
Peroxiredoxins (Prxs) are novel group proteins with efficient antioxidant capacity, and some of them also have effects on cell proliferation, differentiation, apoptosis, and chemotherapy and radiotherapy resistance. Altogether six distinct Prxs expressions were investigated in histological samples o...
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PMID: 20712014
PDF is available here.
PDF is available here.
Abstract
We summarize recent data, reported by different research groups, suggesting that the dopamine (DA) D1 receptor forms heteromers with receptors of the same family and with structurally and functionally divergent receptors.
DA D1 and D3 receptors and glutamate NMDA receptors regulate rewarding mechani...
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PMID: 20684667
PDF is available here.
PDF is available here.
Abstract
We utilized pre- and post-intervention samples from a randomized double blinded trial with 50-57-year-old women. The intervention included the year-long use of either HRT preparation (2 mg 17β-estradiol, E₂; 1mg norethisterone acetate, NETA, n=10) or placebo (CO, n=9). Microarray technology and q...
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PMID: 20724185
PDF is available here.
PDF is available here.
Parasitology Research (107)5 2010
Abstract
Albendazole is a benzimidazole drug which can be used to treat liver fluke (Fasciola hepatica) infections. Its mode of action is believed to be the inhibition of microtubule formation through binding to β-tubulin. However, F. hepatica expresses at least six different isotypes of β-tubulin, and thi...
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PMID: 20676683
PDF is available here.
PDF is available here.
Biochemical Genetics (48)9-10 2010
Abstract