Abstract
The conjunctiva's bacterial profile at birth and 1 week after Credé's gonoccocal ophthalmic prophylaxis was evaluated over a 3-month period using culture and direct immunofluorescence. Thirty-four neonates received 10% silver vitellinate and 42 sterile saline. Chlamydia trachomatis was recovered...
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PMID: 18318948
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Abstract
The tintinnid ciliate Stenosemella pacifica Kofoid and Campbell, 1929 was occasionally recorded from the pelagial of temperate, subtropical, and tropical neritic waters. Since its cytological features were unknown, the species is redescribed from material collected in the pelagial of the Irish Sea,...
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PMID: 18318859
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Abstract
The oxytrichid ciliate Architricha indica nov. gen., nov. sp., isolated from the river Yamuna, Delhi, shows a new combination of characters. It possesses a flexible body, 18 frontal-ventral-transverse (FVT) cirri, 3 right and 2 left marginal cirral rows, 6 dorsal bristle rows and 3 caudal cirri (CC)...
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PMID: 17070749
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Abstract
The morphology and infraciliature of the type species of the ciliate genus Trachelostyla, T. pediculiformis (Cohn, 1866) Borror, 1972, collected from the Bohai Sea near Tianjin, north China, were investigated using live observation and protargol impregnation. The Chinese population corresponds well...
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PMID: 17070752
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Abstract
Two epibiotic peritrichs infested the blue crab, Callinectes sapidus, from the Gulf of Mexico, Mississippi, USA. Epistylis callinectes n. sp. was isolated from the epipods of maxillipeds, bases of gill-cleaning setae, and gills, and Epistylis clampi n. sp. was isolated from the exterior surfaces of...
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PMID: 16579810
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Abstract
To determine the feasibility of using x-ray fluorescence (XRF) to detect the presence of silver in the mammary gland of dairy cows injected with mild silver protein suspension. The XRF spectroscopy was conducted on cadaver udders with and without mild silver protein injected. Spectral analysis was p...
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PMID: 15971676
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Abstract
These results demonstrate that medullary bone matrix accumulates keratan sulphate in the globular structures, which are the foci for calcification, and eventually in the calcified areas. This suggests that keratan sulphate containing sulphur is maintained in the calcified matrix. These results indic...
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PMID: 15695482
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Abstract
The infraciliature and myoneme system of Campanella umbellaria were revealed using the protargol impregnation technique. The main characteristics of the infraciliature are the peristomial ciliary rows (haplokinety and polykineties), which make four and a half turns around the peristomial disc before...
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PMID: 15164366
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Abstract
Two euplanktonic ciliates, Urotricha psenneri n. sp. (Prostomatida) and Amphileptus piger (Vuxanovici, 1962) n. comb. (Pleurostomatida), were discovered in the surface plankton of the oligotrophic Lake Traunsee in Austria. Their morphology and infraciliature were studied in live cells as well as in...
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PMID: 15666725
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Abstract
A new pleurostomatid genus Apoamphileptus is described, which is diagnosed as: Belonging to the Amphileptidae with spica on right side; on each side of the cell, a single perioral kinety, which encircles the cytostome and does not extend to the posterior end of the cell; somatic kineties of both sid...
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PMID: 15666718
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Abstract
The authors review current views on the treatment of acute rhinitis (AR) in infants and present their experience in AR treatment of 30 infants aged from 3 days to 1 year. Combined treatment with Physiomer baby quickly eradicated inflammation. The drug eliminates crusts and nasal discharge allowing a...
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PMID: 15699987
PDF is available here.
Abstract
It has been suggested that species loss from microbial groups low in diversity that occupy trophic positions close to the base of the detrital food web could be critical for terrestrial ecosystem functioning. Among the protozoans within the soil microbial loop, ciliates are presumably the least abun...
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PMID: 12689714
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Abstract
Seven species of marine fishes from coastal regions of the Yellow Sea were examined for ectoparasitic trichodinids (Protozoa, Ciliophora). A total of seven species belonging to the genus Trichodina Ehrenberg, 1830, including two new species, occurred as gill parasites. These were T. rectispina n. sp...
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PMID: 11912338
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Abstract
A marine planktonic ciliate, belonging to the genus Askenasia Blochmann, 1895, is described with notes on its distribution and ecology. Specimens of this new species were collected from four coastal sites across the northern hemisphere. Samples were collected between March and December, from depths...
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PMID: 12425531
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Abstract
These results strongly suggest that medullary bone calcification progresses by the coalescence of calcified nodules and that sulfated glycosaminoglycans play an important role for the regulation of globular calcification.
Copyright 2001 Wiley-Liss, Inc....
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PMID: 11505368
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Abstract
This study examined the occurrence and morphological features of serous-type cells in human sublingual gland, using immunocytochemistry for lysozyme. Lysozyme-positive cells usually formed demilunes and occasionally their own acini. They were also found among cells of an intercalated duct and in its...
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PMID: 11575426
PDF is available here.
Abstract
In the present work are reported investigations on the formation and chemical nature of the chorion of Ascidia malaca oocytes. The ultrastructural observations have shown that both follicle cells and test cells play a key role on chorion formation. At the beginning of vitellogenesis (stage I), chori...
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PMID: 11686403
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Abstract
The morphology and infraciliature of the rare heterotrichid ciliate Copemetopus subsalsus Villeneuve-Brachon, 1940 were studied in live and protargol-impregnated specimens and also by scanning electron microscopy. It is characterized by a dumbbell-shaped macronucleus, a specific dorsal brush cilia,...
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PMID: 12095107
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Abstract
The research on ciliates, flagelates and opalinates have been widespread by the utilization of techniques employing silver impregnation (protargol), modified by several authors. However, these are time consuming and its results are variable. The present work is a variant of the technique described b...
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PMID: 11188871
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Abstract
We found that both heparan sulfates and chondroitin sulfates (A and/or C) were detected in association with the basal lamina of smooth muscle cells and the external surface of elastic lamina, and in the latter heparan sulfates were frequently recognized as a mass, whereas chondroitin sulfates (A and...
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PMID: 9853295
PDF is available here.
Abstract
We classified them into two immature types (I, II) and two mature types (I, II). Their distinctive features were the following: 1) relatively small (0.5-1 microm) secretory granules and well-developed basal rough endoplasmic reticulum for the immature types; 2) larger (1-2 microm) secretory granules...
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PMID: 9756097
PDF is available here.
Abstract
We conclude that mucous neck cells are precursor cells of chief cells. Mucous neck cells, intermediate cells, and chief cells are in the same differentiating cell lineage....
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PMID: 9634604
PDF is available here.
Abstract
To determine the state of fibroblast during the process of development of hypertrophic scar (HS), 40 specimens of HS in different periods were collected. The expressions of prolifrating cell nuclear antigen (PCNA) and Ag-protein in nucleolar organizer regions (Ag NORs) as well as the content of tota...
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PMID: 10374605
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Abstract
Intracytoplasmic lumina (ICL) in human oviduct epithelium were investigated with transmission electron microscopy. ICL were found in 43 out of 60 cases examined. They were ultrastructurally characterized by microvilli lining the lumina, periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP)...
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PMID: 9090027
PDF is available here.
Abstract
An electron microscopic analysis with specific histochemical stainings for acidic glycoconjugates was carried out to examine the endothelium lining blood vessels of the rat spleen. Histochemical staining performed was the postembedding high or low iron diaminethiocarbohydrazide-silver protein-physic...
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PMID: 8937640
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Abstract
We emphasize the lack of established effectiveness and potential toxicity of these products....
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PMID: 8632503
PDF is available here.
Abstract
We conclude that glycogen in inclusions of C. trachomatis originates in the organisms themselves....
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PMID: 7545158
PDF is available here.
Abstract
The expression of various melanogenic proteins, including tyrosinase, the tyrosinase-related proteins 1 (TRP1) and 2 (TRP2/DOPAchrome tautomerase), and the silver protein in human melanocytes was studied in six different human melanoma cell lines and compared to a mouse derived melanoma cell line. A...
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PMID: 7659683
PDF is available here.
Abstract
It has been previously demonstrated that the mixture of Cu, Au and Ag impaired significantly the clinical and biochemical disorders induced by adjuvant arthritis in the rat. The efficiency of this mixture and of each component was tested on the same animal model. Treated rats were injected daily wit...
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PMID: 7741265
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Abstract
These results indicated that, depending on the fixative used, B. hominis contained PAS- or AB-reactive carbohydrates. At the electron microscopic level, ultrathin sections of B. hominis were stained with periodic acid methenamine silver (PA-MS) or periodic acid thiocarbohydrazide-silver proteinate (...
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PMID: 7537145
PDF is available here.
Abstract
The glycogen content of several types of supporting cells in the organ of Corti of the rat was demonstrated histochemically at the ultrastructural level using the periodic acid-thiocarbohydrazide-silver proteinate method. The development of the glycogen stores was assessed by means of a semiquantita...
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PMID: 7751611
PDF is available here.
Abstract
Hen egg-white lysozyme is known to be fungicidal to blastoconidia of Candida albicans under defined in vitro conditions. This lethal action leads to changes in the layering of cell wall and to plasmolysis, caused by unremitting accumulation of wall-like material between the yeast cell wall and cytop...
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PMID: 8402534
PDF is available here.
Abstract
A dual staining method was established for the histochemical detection of neutral carbohydrates and deoxyribonucleic acid (DNA) in light microscopy. The method consisted of combined periodic acid-thiocarbohydrazide-silver protein-physical development (PA-TCH-SP-PD) and hot hydrochloric acid (H-HCl)-...
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PMID: 7682321
PDF is available here.
Abstract
We evaluated the clinical efficacy and safety of a Foley catheter coated with silver-protein (ProAg catheter) in the prevention of catheter-associated bacteriuria. ProAg catheter significantly reduced the incidence of extraluminal catheter-associated bacteriuria compared with usual latex Foley cathe...
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PMID: 8506804
PDF is available here.
Abstract
We investigated the possible existence of mucin in lymphatic vessels in cholesteatoma perimatrix using the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method. Histochemical staining distinguished two types of lymphatic vessels, one of which contained PA-TCH-SP reacting substance s...
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PMID: 8396397
PDF is available here.
Abstract
Comparable antimicrobial and disinfecting action of decamethoxine and silver preparations on pathogens of chronic purulent otitis media (CPOM) was studied. The clinical isolates of staphylococci proved to be most sensitive to decamethoxine whose MBcC conformed to 16.5 micrograms/ml. The antimicrobia...
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PMID: 1304133
PDF is available here.
Abstract
Lead tetraacetate-thiocarbohydrazide-silver proteinate reaction sequence for light microscopy of polysaccharides was evaluated on Carnoy's fixed rat liver sections. The results of this evaluation suggest that, on the light microscopic level, the lead tetraacetate-thiocarbohydrazide-silver proteinate...
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PMID: 1489842
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Abstract
Cryofixation is generally believed to provide optimal tissue preservation. However, certain post-embedding cytochemical reactions, such as high-iron diamine (HID) staining for sulfated glycoconjugates, are not applicable to cryofixed and freeze-substituted tissues. In the present study, the HID tech...
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PMID: 1380528
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Abstract
We used silver acetate as an ion donor. This new method allowed physical development under normal lighting conditions, and resulted in the reduction of background staining even after amplification. As the developer did not contain gum arabic, troublesome treatment was not necessary. The time require...
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PMID: 1377740
PDF is available here.
Abstract
The pathogenesis of the nodular lesion in diabetic glomerulosclerosis is described in association with fibrils. Thirteen diabetic patients with glomerular nodular lesions and 9 diabetics without the nodules were examined by electron microscopy using periodic acid-thiocarbohydrazide-silver proteinate...
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PMID: 1329309
PDF is available here.
Abstract
The authors conducted an open-label nonrandomized parallel trial to examine whether the preoperative application of povidone-iodine to the ocular surface reduces the incidence of endophthalmitis after intraocular surgery. During an 11-month period, topical 5% povidone-iodine was used to prepare the...
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PMID: 1775308
PDF is available here.
Abstract
A method has been developed for the histochemical demonstration of unsaturated lipids in light microscopy. It is a peracetic acid-thiocarbohydrazide-silver protein sequence followed by a physical development procedure. In the present study on paraffin and cryostat sections of liver, brain and ovary,...
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PMID: 1717409
PDF is available here.
Abstract
A new visualization principle for the detection of cerium phosphate reaction product of phosphatases in light microscopy is described. The new mode is based on the conversion of cerium phosphate into cerium oxalate. The latter is able to react with OsO4. In this way osmium black is formed, staining...
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PMID: 1718603
PDF is available here.
Abstract
A modification of Bodian's protargol-S technique is done on 7 microns sections of decalcified bone. Light microscopic results are greatly improved when compared to either ground bone sections or decalcified bone stained routinely with hematoxylin and eosin.
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PMID: 1717001
PDF is available here.
Abstract
We have made allows its use for both free-living cells (protozoa) and cells grown in culture (nerve cells). The significant modifications are 1) different fixation, 2) postfixation with Cajal's formol-bromide, 3) changes in the duration of the impregnation steps technique and 4) elimination of metal...
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PMID: 1716164
PDF is available here.
Abstract
We used in our studies as a model the regenerating liver. Among extranucleolar structures affinity to silver interchromatin granules and weakly perichromatin granules was demonstrated. Deposits of silver grains were always located within dense fibrillar components and fibrillar centers. Our study do...
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PMID: 1794435
PDF is available here.
Abstract
Functional differentiation of the notochord in rhesus monkey embryos at stages 11-12 (25-28 days of gestation) was analyzed by means of ultrastructural cytochemistry. The notochordal cells exhibited well developed Golgi complexes, rough endoplasmic reticulum, mitochondria, and numerous coated vesicl...
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PMID: 2285159
PDF is available here.
Abstract
Our results suggest that: 1) most dense bodies in the apical cytoplasm belong to the secretory granules discharged into the ventricular cavity, 2) dilated cisternae of the ER serve as the storage site of the substance which eventually becomes the secretory product, however, contents of the ER are no...
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PMID: 1699191
PDF is available here.
Abstract
We have established an efficient method for the histochemical demonstration of protein-bound amino groups by light microscopy, using a ninhydrin or alloxan-thiocarbohydrazide-silver proteinate (NHY or ALX-TCH-SP) sequence followed by a physical development (PD) procedure. As a result of the present...
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PMID: 2355178
PDF is available here.
Abstract
Basophilic bodies of skeletal muscles from two patients with hypothyroidism were examined by enzyme histochemistry and ultrastructural study of ultrathin sections stained with periodic-acid-thiocarbohydrazide-silver proteinate for polysaccharides. Some additional characterizations of basophilic bodi...
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PMID: 2478444
PDF is available here.