Abstract
Recurrence of peste des petits ruminants (PPR) was diagnosed in the United Arabian Emirates in several wild ruminants confirmed by morphological, immunohistochemical, serological and molecular findings. Phylogenetic analysis revealed that the virus strain belongs to lineage IV, which is different to...
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PMID: 20067659
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Abstract
We discuss available potent PPR vaccines and the future possibility of developing new-generation vaccines against PPR....
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PMID: 20624051
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Abstract
A one-step real-time Taqman RT-PCR assay (RRT-PCR) for peste des petits ruminants virus (PPRV) was developed to detect the four lineages of PPRV by targeting the nucleoprotein (N) gene of the virus. This new assay was compared to a conventional RT-PCR on reference strains and field materials. Quanti...
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PMID: 20116402
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Abstract
Retrospective quantitative analysis of epidemiological data on peste des petits ruminants (PPR) from the Department of Animal Husbandry and Veterinary Sciences in Karnataka, southern India, revealed significant information about the disease in this area. In the nine years between April 1998 and Marc...
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PMID: 20462160
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Abstract
Counter immnuo-electrophoresis (CIEP) and Competitive ELISA (C-ELISA) tests were employed for seroprevalence of Peste des Petits Ruminants (PPR) infection in Sudan. The result of both tests showed high prevalence of PPRV antibodies in sheep and goats sera collected from six different regions of Suda...
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PMID: 19798772
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Zhiliang Wang,
Jingyue Bao,
Xiaodong Wu,
Yutian Liu,
Lin Li,
Chunju Liu,
Longciren Suo,
Zhonglun Xie,
Wenji Zhao,
Wei Zhang,
Nan Yang,
Jinming Li,
Shushuang Wang and
Junwei Wang
Abstract
Serologic and molecular evidence indicates that peste des petits ruminants virus (PPRV) infection has emerged in goats and sheep in the Ngari region of southwestern Tibet, People's Republic of China. Phylogenetic analysis confirms that the PPRV strain from Tibet is classified as lineage 4 and is clo...
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PMID: 19193278
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Abstract
These results clearly indicate that PPR is prevalent throughout Pakistan....
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PMID: 19284055
PDF is available here.
Abstract
This paper presents the results of a seroepidemiological study carried out between July 2006 and March 2007 to detect the presence of antibodies to peste des petits ruminants (PPR) virus in randomly collected serum samples from sheep and goats in southern peninsular India. The authors used a competi...
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PMID: 19284053
PDF is available here.
Abstract
Peste des petits ruminants made its first clinical appearance in Pakistan during an epidemic in Punjab Province in 1991, but its level of activity in subsequent years was never systematically recorded. In 2003, 2004 and 2005 teams trained in participatory disease surveillance methods visited a large...
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PMID: 19284054
PDF is available here.
Jing-Yue Bao,
Zhi-Liang Wang,
Lin Li,
Wen-Ji Zhao,
Long-Ci-Ren Suo,
Jin-Ming Li,
Ying-Li Wang,
Xiao-Dong Wu,
Chun-Ju Liu,
Yu-Tian Liu,
Xiao-Jing Yu and
Yong-Mei Yang
Abstract
The N gene and genome promoter nucleotide sequence of a Chinese Peste des petits rumiants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The length of N gene was 1689 nucleotides with a single open reading frame (ORF). The nucleotide and deduced amino acid sequence was compared with th...
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PMID: 19226956
PDF is available here.
Abstract
Monoclonal antibody-based competitive ELISA (C-ELISA) has been used for the specific measurement of antibodies to peste des petits ruminants (PPR) viruses in sheep, goats, cattle and buffalo. Serum samples from sheep (n = 232), goats (n = 428), cattle (n = 43), buffalo (n = 89) were tested. The anim...
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PMID: 18716909
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Abstract
Peste des petits ruminants (PPR) disease was confirmed in West African Dwarf goats. They were managed symptomatically with antibiotics and antidarrhoeics. Following clinical recovery, faeces were collected every week from 40 recovered goats to monitor excretion of the PPR virus haemagglutinins in th...
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PMID: 18716908
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Abstract
Peste des petits ruminants (PPR) is one of the most economically important diseases affecting sheep and goats in India. An immunofiltration-based test has been developed using either mono-specific serum/monoclonal antibodies (mAb) prepared against a recombinant truncated nucleocapsid protein of rind...
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PMID: 18182256
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Abstract
These results provide further insights into the feasibility of applying microcarrier cell culture technology to produce PPR vaccine in Vero cells as well as in the alternative use of single-cell suspension cultures of BHK-21A, significantly simplifying the existing production process....
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PMID: 18486286
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Abstract
We investigated the seroprevalence, and risk factors, of PPR in sheep and goat flocks from five different governorates (Irbid, Jarash, Ajloun, Mafraq and Zarka) located in Northern Jordan. Serum samples from 929 and 400 sheep and goats, respectively, corresponding to 122 sheep flock and 60 goats flo...
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PMID: 18291541
PDF is available here.
Abstract
AGPT and HA tests were employed for rapid diagnosis of PPRV infection in sheep and goats in Sudan. Forty lymph nodes and spleen samples from suspected cases of PPR in both sheep and goats were examined by AGPT and HA tests for detection of PPRV antigen. Viral antigen was detected from (77.5%) of the...
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PMID: 18509945
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Abstract
Present study was designed to investigate the prevalence and mortality (%) caused by Peste des Petitis Ruminant (PPR) and its possible association with abortion in goat flocks at different areas of Pakistan. A total of 140 animals were samples in the population of 650 which was having 185 deaths (Mo...
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PMID: 18509938
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Abstract
Concurrent infection with peste des petits ruminants virus (PPRV) and pestivirus was diagnosed in stillborn twin lambs. With the flock history, the findings of epidermal syncytial cells and necrotizing bronchitis/bronchiolitis prompted testing for PPRV infection, and PPRV antigen was detected by imm...
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PMID: 18424832
PDF is available here.
Abstract
In this study, a rapid and specific TaqMan-based, one-step real-time quantitative reverse transcription PCR (qRT-PCR) has been described for the detection of peste des petits ruminants virus (PPRV). Primers and probe were designed based on the nucleocapsid protein gene sequence. The real-time qRT-PC...
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PMID: 18243345
PDF is available here.
Abstract
Peste des petits ruminants (PPR) is a highly contagious and economically important viral disease of goats and sheep. A homologous Vero cell-based attenuated PPR vaccine developed in our laboratory and used extensively throughout the country, is available for control of PPR. The presently used qualit...
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PMID: 17644410
PDF is available here.
Abstract
We report on the cloning of the Peste des Petits Ruminants virus (PPRV) N gene into the baculovirus vector and the expression of the protein in insect cells. By electron microscopy observation, we have shown that this recombinant PPRV N forms nucleocapsid-like particles in insect cells in the absenc...
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PMID: 17875339
PDF is available here.
V Michaud,
P Gil,
O Kwiatek,
S Prome,
L Dixon,
L Romero,
M-F Le Potier,
M Arias,
E Couacy-Hymann,
F Roger,
G Libeau and
E Albina
Abstract
In tropical countries the diagnosis of viral infections of humans or animals is often hampered by the lack of suitable clinical material and the necessity to maintain a cold chain for sample preservation up to the laboratory. This study describes the use of filter papers for rapid sample collection,...
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PMID: 17714797
PDF is available here.
Abstract
Peste-des-petits-ruminants virus (PPRV) and rinderpest virus (RPV) are two morbilliviruses of economic relevance in African and Asian countries. Although efficient vaccines are available for both diseases, they cannot protect the animals before 14 days post-vaccination. In emergencies, it would be d...
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PMID: 17622636
PDF is available here.
Abstract
Peste des petits ruminants (PPR) is a highly contagious animal disease caused by a virus in the genus Morbillivirus, family Paramyxoviridae. This infection is responsible for high morbidity and mortality in sheep and goats and in some small wild ruminant species. The huge number of small ruminants,...
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PMID: 17399862
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Abstract
The nucleocapsid (N) protein of all morbilliviruses has a highly conserved central region that is thought to interact with and encapsidate the viral RNA. The C-terminal third of the N protein is highly variable among morbilliviruses and is thought to be located on the outer surface and to be availab...
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PMID: 17554036
PDF is available here.
Abstract
We report and describe in detail the first peste des petits ruminants endemic in Kirikkale Province, Central Anatolia of Turkey. In conclusion, these previously unreported pathologic findings in natural peste des petits ruminants virus infection establish a basis for resemblance to other morbillivir...
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PMID: 17606509
PDF is available here.
Abstract
We constructed a PPRV minigenome and studied its expression in transfected cells. As for other morbilliviruses, the minimum requirements for minigenome rescue were shown to be the cis-acting elements of the genome (GP) and antigenome (AGP) promoters as well as the three trans-acting helper proteins...
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PMID: 17350130
PDF is available here.
Abstract
These results suggest that the indirect ELISA may be a good alternative tool to competitive ELISA for seroepidemiological surveys....
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PMID: 17216310
PDF is available here.
Abstract
We observed 15 goats for 9 days after subcutaneous infection with 10(3) TCID(50) with isolates of peste-des-petits ruminants virus from Africa and India and five concurrent, uninfected control goats. Typical clinical signs of the infection were present in all 15 infected goats by day 8 and in most b...
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PMID: 17064800
PDF is available here.
Abstract
Different isolates of peste-des-petits-ruminants virus (PPRV) from outbreaks in Africa and India were investigated for virulence in West African dwarf goats in the Ivory Coast. Six groups of five animals received a virulent suspension of various strains of virus at a concentration of 10(3) TCID(50)/...
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PMID: 16310383
PDF is available here.
Abstract
The occurrence of outbreaks of peste des petits ruminants (PPR) in three districts of Tajikistan is described. The causal strain (PPR Tajikistan) was characterized and the sequence of its N gene was compared with that of 43 other strains isolated since 1968 in Africa, the Middle East and Asia. The s...
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PMID: 17321539
PDF is available here.
Abstract
Peste des petits ruminants is a highly contagious viral disease of small ruminants making its diagnosis difficult from the similar symptoms of Rinderpest. Computer based prediction algorithms was applied to identify antigenic determinants on the nucleocapsid (N) protein of PPRV. Specificity and anti...
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PMID: 16962260
PDF is available here.
Abstract
The complete nucleotide sequence of the nucleocapsid (N) protein of the peste-des-petits ruminants vaccine virus (PPRV Sungri/96) belonging to the Asian lineage was determined. The gene was 1692 nucleotides in length and encoded a polypeptide of 525 amino acids. The PPRV Sungri/96 N gene has a nucle...
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PMID: 17139548
PDF is available here.
Abstract
Safety and immunogenicity of an experimental combined vaccine comprising attenuated strains of Peste des Petits ruminants virus (PPRV) and goat poxvirus (GTPV) was evaluated in goats. Goats immunized subcutaneously with 1 ml of vaccine consisting of 10(3) TCID(50) of each of PPRV and GTPV were monit...
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PMID: 16828938
PDF is available here.
Abstract
A single-tube one-step multiplex RT-PCR was standardized to amplify both 337 bp and 191 bp fragments of N and M genes of peste des petits ruminants virus (PPRV), respectively, and only a 337 bp fragment of N gene of Rinderpest virus (RPV). The RT-PCR using purified viral RNA was easily adopted for d...
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PMID: 16838207
PDF is available here.
Abstract
The ability of the attenuated vaccine 75/1 of peste des petits ruminants to interfere with rinderpest vaccination in cattle was investigated experimentally. Young cattle (93) were selected and tested as being negative for antibodies against RP or PPR viruses. These were vaccinated with the peste des...
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PMID: 16757064
PDF is available here.
Abstract
The matrix (M) protein of paramyxoviruses forms an inner coat to the viral envelope and serves as a bridge between the surface glycoproteins (F and H) and the ribonucleoprotein core. Previously, a marker vaccine (RPV-PPRFH) was produced for the control of peste des petits ruminants (PPR) disease, wh...
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PMID: 16760405
PDF is available here.
Abstract
The M gene nucleotide sequence of an Indian peste-des-petits ruminants (PPRV) vaccine virus ("PPRV Sungri/96") belonging to Asian lineage was determined. The gene is 1476 nucleotides long with a single open reading frame (ORF). The nucleotide and predicted amino acid sequence was compared with the h...
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PMID: 16297575
PDF is available here.
Abstract
The complete nucleotide sequence of the large polymerase (L) protein of the peste-des-petits ruminants (PPR) vaccine virus (PPRV Sungri/96) belonging to the Asian lineage was determined. The gene was 6643 nucleotides in length from the gene-start to the gene-end and encoded a polypeptide of 2183 ami...
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PMID: 16052284
PDF is available here.
Abstract
The authors provide a report on the surveillance of rinderpest virus (RPV) and peste des petits ruminants virus (PPRV) in the wildlife population in Côte d'Ivoire. For this purpose, 266 animals from nine different species, selected according to susceptibility and abundance, were captured and sample...
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PMID: 16642757
PDF is available here.
Abstract
A questionnaire-survey data indicated that 26% of 276 farmers reported the presence of respiratory disease in their herds in 2001. The incidence was perceived as "high" in small ruminants and camels, but as "low" in cattle. Simultaneously, 2815 serum samples from camels (n=628), cattle (n=910), goat...
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PMID: 15967242
PDF is available here.
Abstract
A survey was carried out in two goat herds during a single peste des petits ruminant (PPR) outbreak. Clinical examination showed that animals belonging to the West African Dwarf species had severe symptoms while those belonging to the West African long-legged species had mild symptoms. To confirm an...
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PMID: 16050518
PDF is available here.
Abstract
Effect of virulent and attenuated peste des petits ruminants (PPR) virus on the immune response to nonspecific antigen (ovalbumin) was investigated. Clinical and serological responses were monitored in goats administered with ovalbumin concurrently with either PPR vaccine or virulent virus. Study sh...
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PMID: 16188317
PDF is available here.
Abstract
We developed a rapid competitive enzyme-linked immunosorbent assay (rapid c-ELISA) for the diagnosis and surveillance of PPR. This assay detects PPR virus (PPRV) antibodies in serum samples by quantifying the amount of monoclonal antibody (MAb) P-3H12 after 30 min of incubation of a serum-MAb conjug...
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PMID: 15817764
PDF is available here.
Abstract
Peste des petits ruminants (PPR) is an acute febrile viral disease of goats and sheep characterised by mucopurulent nasal and ocular discharges, necrotising and erosive stomatitis, enteritis and pneumonia. The disease is endemic in India and causes large economic losses each year due to the high rat...
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PMID: 15861876
PDF is available here.
Abstract
A sandwich ELISA test using PPR specific monoclonal antibody (clone 4G6) to an epitope of nucleocapsid protein has been developed. The test uses polyclonal sera to capture the antigen from clinical samples (swabs and tissues). Captured antigens from clinical samples are detected using PPR specific m...
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PMID: 15503204
PDF is available here.
Abstract
Peste des petits ruminants (PPR) is an acute, febrile viral disease of small ruminants, caused by a virus of the genus Morbillivirus. PPR and rinderpest viruses are antigenically related and need to be differentiated serologically. In the present study, 23 mouse monoclonal antibodies were produced b...
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PMID: 15563110
PDF is available here.
Abstract
By the end of March 2002, a collection of some 200 gazelles, kept under semi range conditions in Saudi Arabia, was hit by a highly fatal peracute disease. The morbidity rate was 51% while the case mortality rate was 100%. Clinico-pathological and virological investigations were carried out. A virus...
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PMID: 15030603
PDF is available here.
Abstract
Peste des petits ruminants (PPR) is an acute febrile, viral, disease of small ruminants with great economic importance. A competitive-ELISA (c-ELISA) test was developed for detection of antibodies to PPR virus in the sera samples of goats and sheep. The test uses monoclonal antibody to a neutralizin...
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PMID: 14738776
PDF is available here.
Abstract
We produced this reagent in goats using attenuated local strain of PPRV. The quality was assured to meet the internationally required levels of potency and sterility. The titer of the product was 1024 as evaluated by virus neutralization (VN) and agar gel immunodiffusion (AGID) tests. In its final f...
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PMID: 15724381
PDF is available here.
Abstract
Peste des petits ruminants (PPR) is a widely spread viral disease with limited sporadic outbreaks particularly among small ruminants. The diagnosis depends on the detection of antibodies by commercially available expensive reagents. The aim of this work was to prepare and evaluate antigen from an at...
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PMID: 16734131
PDF is available here.
Abstract
This report describes the pathological and immunohistochemical findings in naturally infected lambs from three outbreaks of peste des petits ruminants in Mugla and Aydin provinces of the Ege district of Turkey. At necropsy, ulcerative stomatitis, catarrhal or fibrinous bronchopneumonia, and acute ca...
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PMID: 15276853
PDF is available here.
Abstract
An experimental competitive enzyme-linked immunosorbent assay (morbillivirus cELISA) using a recombinant N antigen (rRPV N) expressed in a baculovirus and a ruminant morbillivirus (RPV and PPRV)-specific monoclonal antibody (P-13A9) was developed for simultaneous detection of rinderpest virus (RPV)...
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PMID: 14516703
PDF is available here.