Abstract
These results highlight the potential risk posed by these environmental elements in the spread of infection and the need for implementation of adequate management practices in order to minimize this risk....
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PMID: 20653991
PDF is available here.
Abstract
These results suggested that B. suis bv. 2 may use strategies similar to other smooth brucellae to facilitate intracellular multiplication and the development of chronic infections. To our knowledge, this is the first report of the analysis of gene expression profile in hosts infected with B. suis b...
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PMID: 21115167
PDF is available here.
Abstract
We found a higher number of H. parasuis strains (16 strains) within a given farm than previously thought. Overall, more H. parasuis diversity was found in piglets from non-vaccinated sows than in those from vaccinated sows. These results indicate that vaccination of sows in a farm delays the coloniz...
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PMID: 20447775
PDF is available here.
Chairul A Nidom,
Ryo Takano,
Shinya Yamada,
Yuko Sakai-Tagawa,
Syafril Daulay,
Didi Aswadi,
Takashi Suzuki,
Yasuo Suzuki,
Kyoko Shinya,
Kiyoko Iwatsuki-Horimoto,
Yukiko Muramoto and
Yoshihiro Kawaoka
Abstract
We conducted surveillance during 2005-2009. We found that 52 pigs in 4 provinces were infected during 2005-2007 but not 2008-2009. Phylogenetic analysis showed that the viruses had been introduced into the pig population in Indonesia on at least 3 occasions. One isolate had acquired the ability to r...
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PMID: 20875275
PDF is available here.
Abstract
We investigated the expression of immunoglobulin A (IgA) in porcine salivary gland and its relationship with restraint stress in pigs. IgA was expressed in plasma cells in pig salivary gland, as confirmed by immunohistochemical staining. IgA was also detected in pig saliva itself by ELISA, and saliv...
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PMID: 20467204
PDF is available here.
Abstract
Liver lesion prevalence in slaughtered finishing pigs in the Netherlands remained relatively high from the mid-1990s until 2004, although sufficient measures existed to control the main cause, an infection with the roundworm Ascaris suum. In July 2004 a new incentive mechanism was installed to induc...
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PMID: 19819576
PDF is available here.
Abstract
A loop-mediated isothermal amplification (LAMP) assay was developed specifically for detection and differentiation of pseudorabies virus (PRV). One group of primers was designed to detect wild-type strains (i.e., strains with the gE gene) and the other group of primers was designed to detect both PR...
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PMID: 20691214
PDF is available here.
Abstract
We performed genetic analyses of the HA gene by comparing the 2009 H1N1 virus with seasonal human and swine viruses. We analyzed sequences of 116 2009 H1N1 viruses, and obtained 1457 seasonal H1N1, 365 swine H1, and 1332 2009 H1N1 viruses from the database. Selection pressure for the 2009 H1N1 virus...
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PMID: 20598336
PDF is available here.
Abstract
Serum samples from 2970 (1400 sows, 1570 fattening) pigs, from 100 farms in the 10 main swine production regions in Spain were tested for antibodies against T. gondii by the modified agglutination test (MAT). Antibodies to T. gondii (MAT 1:25 or higher) were detected in 492 pigs (16.6%, 9.7% in fatt...
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PMID: 20601110
PDF is available here.
Abstract
These results were confirmed by restriction enzyme analysis. With regard to feces and saliva samples, 15/25 (60%) and 16/25 (64%) were positive for Helicobacter spp. PCR, respectively but all were negative in H. suis and H. pylori specific PCR....
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PMID: 20706029
PDF is available here.
Marek J MJ Slomka,
Anstice L E AL Densham,
Vivien J VJ Coward,
Steve S Essen,
Sharon M SM Brookes,
Richard M RM Irvine,
Erica E Spackman,
Jonathan J Ridgeon,
Rebecca R Gardner,
Amanda A Hanna,
David L DL Suarez and
Ian H IH Brown
Abstract
There is a requirement to detect and differentiate pandemic (H1N1) 2009 (H1N1v) and established swine influenza A viruses (SIVs) by real time reverse transcription (RRT) PCR methods.
First, modify an existing matrix (M) gene RRT PCR for sensitive generic detection of H1N1v and other European SIVs. S...
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PMID: 20716157
PDF is available here.
Abstract
The aim of this study was to develop in situ hybridization for detection of Mycoplasma hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from pigs with polyserositis. M. hyorhinis was isolated from the spleen (2 pigs) and pericardium (1 pig). M. hyorhinis DNA was detected 16 out of 20 pigs...
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PMID: 20424392
PDF is available here.
Abstract
Traditionally, the routine artificial digestion test is applied to assess the presence of Trichinella larvae in pigs. However, this diagnostic method has a low sensitivity compared to serological tests. The results from artificial digestion tests in Switzerland were evaluated over a time period of 1...
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PMID: 20067658
PDF is available here.
Abstract
We analyse which local landscape characteristics are associated with the presence of toxoplasmosis in wild boar, Sus scrofa, on the island of Corsica, France. Meat juice samples from 1399 wild boars collected during two hunting seasons were tested for T. gondii antibodies using the modified agglutin...
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PMID: 20096148
PDF is available here.
Abstract
We describe for the first time the sex and origin related differences in the skin-test response to mycobacterial antigens (bPPD and aPPD) and to a non-mycobacterial antigen (PHA, a plant derived mitogen) in wild and farmed wild boar, and used a small sample of known M. bovis infected wild boar to es...
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PMID: 20633938
PDF is available here.
Abstract
Taenia solium is the causative agent of neurocysticercosis, a disease responsible for substantial human morbidity and mortality. It is a zoonotic parasite, involving pigs as intermediate hosts. The parasite's full life cycle is restricted to poor people in developing countries. Attempts to date to c...
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PMID: 20470777
PDF is available here.
Abstract
Escherichia coli enterotoxigenic strains produce one or more toxins which action result in production of diarrhea in animals including Man. One of these toxins, STb, has been mainly associated with colibacillosis in swine. Although highly prevalent in pigs with diarrhea, a relation between STb and d...
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PMID: 20367550
PDF is available here.
Abstract
Compared with values in unaffected pigs, serum concentrations of C-propeptide of type II collagen (CPII) and cartilage oligomeric matrix protein were significantly increased and concentrations of carboxy-terminal telopeptide of type II collagen 3/4-length fragment (C2C) and pyridinoline cross-links...
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PMID: 20673095
PDF is available here.
Abstract
OBJECTIVE: To evaluate changes in antimicrobial consumption and productivity by Danish swine farms during 1992 to 2008. SAMPLE POPULATION: All Danish swine farms for antimicrobial consumption data and a representative sample of Danish swine herds for productivity data. PROCEDURES: Antimicrobial cons...
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PMID: 20594073
PDF is available here.
Abstract
Salmonella enterica subsp. enterica serovar 4,[5],12:i:- is a monophasic variant of S. enterica serovar Typhimurium (antigenic formula 4,[5],12:i:1,2). Worldwide, especially in several European countries and the United States, it has been reported among the 10 most frequently isolated serovars in pi...
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PMID: 20472721
PDF is available here.
Abstract
This study evaluated the occurrence of granulomatous lymphadenitis and its association with Actinobacillus spp. in 151 653 slaughtered pigs. Markedly enlarged pulmonary hilar, mediastinal, mandibular or hepatic lymph nodes were detected in 6 castrated males. The cut surfaces showed multifocal yellow...
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PMID: 20885825
PDF is available here.
Linlin Bao,
Lili Xu,
Lingjun Zhan,
Wei Deng,
Hua Zhu,
Hong Gao,
Huihui Sun,
Chunmei Ma,
Qi Lv,
Fengdi Li,
Honglin Chen,
Lianfeng Zhang and
Chuan Qin
Abstract
Our results indicated that pigs, BALB/c mice, and Brandt's voles, but not chickens, ducks, cats, dogs, and rats, could be infected by the novel influenza A (H1N1) virus. Genome sequence alignment results showed that there was one genetic variation (G408T) in the HA gene of Brandt's vole derived viru...
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PMID: 20381552
PDF is available here.
Abstract
Two outbreaks of Streptococcus suis ST7 occurred in humans in 1998 and 2005 in China. PFGE of chromosome restriction fragments found all ST7 isolates to be indistinguishable. Due to the genetic homogeneity of ST7 isolates, development of a rapid sub-typing method with high discriminatory power for S...
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PMID: 20618684
PDF is available here.
Abstract
Many studies have shown transmission of foot-and-mouth disease virus (FMDV) within groups of pigs, even when vaccinated, but only limited information is available on transmission between pens. Three new experiments were carried out in two replicates, which consisted of infectious pigs housed in a ce...
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PMID: 20416264
PDF is available here.
Abstract
We looked at lymphocyte proliferation, reactive oxygen species (ROS), production by neutrophils and monocytes and cytokine production. The soluble beta-glucans Laminarin and Sleroglucan did not activate ROS-production of monocytes and neutrophils while the particulate beta-glucans (beta-glucan from...
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PMID: 20034677
PDF is available here.
Abstract
We used porcine monocytic-derived DC (MoDC) as a model system. The results in this study demonstrate that FcgammaRI, II and III mRNA is expressed by porcine MoDC. Furthermore, we show that FcgammaRII and III are expressed on the cell surface and that F4-IC are internalized by MoDC via FcgammaR. This...
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PMID: 20022123
PDF is available here.
Raimon Duran-Struuck,
Patricia S Cho,
Alexander G S Teague,
Brian Fishman,
Aaron S Fishman,
John S Hanekamp,
Shannon G Moran,
Krzysztof J Wikiel,
Kelly K Ferguson,
Diana P Lo,
Michael Duggan,
J Scott Arn,
Bob Billiter,
Ben Horner,
Stuart Houser,
Beow Yong Yeap,
Susan V Westmoreland,
Thomas R Spitzer,
Isabel M McMorrow,
David H Sachs,
Roderick T Bronson and
Christene A Huang
Abstract
This manuscript reports on five cases of spontaneous myelogenous leukemia, similar to human disease, occurring within highly inbred, histocompatible sublines of Massachusetts General Hospital (MGH) MHC-defined miniature swine. In cases where a neoplasm was suspected based on clinical observations, s...
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PMID: 20079939
PDF is available here.
Abstract
In the United States of America, Aujeszky's disease (pseudorabies) has been eradicated from all domestic swine. Some re-emergence of infection occurred as vaccine use diminished. Sporadic outbreaks have also occurred because of the reservoir of infection in feral swine that have spread across the so...
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PMID: 20444559
PDF is available here.
Abstract
We determined that after 2005 the prevalence of Trichinella spp. in wild boars has increased in this region of Germany. We discuss the potential role of the raccoon dog in the increase in Trichinella spp. prevalence in the sylvatic cycle in this region. We believe that this increase could pose a thr...
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PMID: 20507743
PDF is available here.
Abstract
The seroprevalence of toxoplasmosis in pigs was examined in China's southern Guangdong Province from March 2008 through May 2009. A total of 1,022 serum samples was collected from 12 administrative cities and assayed for Toxoplasma gondii antibodies by enzyme-linked immunosorbent assay test. The ser...
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PMID: 20557218
PDF is available here.
Abstract
In this study, four strains of Japanese Encephalitis Virus (JEV) were isolated from the cerebrospinal fluid of aborted fetuses or stillborn piglets collected randomly from a number of piggeries in central China. The E genes were cloned by RT-PCR and sequenced. Phylogenetic analysis was performed wit...
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PMID: 20213486
PDF is available here.
Abstract
To obtain information about the porcine circovirus type 2 (PCV2) infection status of pigs in Cuba and the probable association of PCV2 with other porcine viruses, tissue samples collected from ill pigs were evaluated using polymerase chain reaction (PCR). The PCR analysis showed that 67.7% of the sa...
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PMID: 20031180
PDF is available here.
Abstract
We present a method for quantification of between holding contact probabilities based on different production types. The data contained seven production types: Sow pool center, Sow pool satellite, Farrow-to-finish, Nucleus herd, Piglet producer, Multiplying herd and Fattening herd. The method also e...
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PMID: 20356640
PDF is available here.
Abstract
Modern commercial swine farrowing crates are typically equipped with slatted iron floor to improve management efficiency (e.g., ease of manure handling, cleanliness of the farrowing crates and hence improved animal hygiene). However, the bare and hard floor surface can impair the welfare of the sow-...
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PMID: 20382437
PDF is available here.
Abstract
We previously reported the detection and partial characterization of a wide range of variable and novel SaV strains of uncertain taxonomic status in Canadian swine. We now report on further genomic characterization of two novel strains to clarify their taxonomic relationship to other swine and human...
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PMID: 20352267
PDF is available here.
Abstract
In Argentina, Trichinella infection has been documented in humans and animals of several provinces since 1930. This zoonotic parasite infection has been recently detected in humans and pigs of a region historically considered as Trichinella-free, suggesting the spread of these pathogens. The aim of...
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PMID: 20153119
PDF is available here.
Abstract
Pseudorabies virus (PRV) is a neurotropic alphaherpesvirus that produces fatal encephalitis in newborn pigs, respiratory disorders in fattening pigs, and reproductive failure in sows. Following primary infection of the respiratory tract, PRV can develop into a systemic infection with dispersion of t...
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PMID: 20219878
PDF is available here.
Abstract
We examined 30 fecal samples from pigs reared on 6 farms in western Japan. Ten pigs (33%) were found to be positive when assessed by polymerase chain reaction. The genotypes were varied and were found to be animal specific (EbpA, H, PigEBITS5) and zoonotic (D, EbpC) in genotype. The observation that...
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PMID: 19725762
PDF is available here.
Abstract
The following case report describes a selenium toxicosis in a pig-fattening farm of two finisher groups. The diseased animals partly showed ataxia and paresis or intense lameness in connection with band-like ablation of the epidermis at the coronary band. Some of them suffered from alopecia. Foot-an...
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PMID: 20464684
PDF is available here.
Abstract
Two genotypes of porcine circovirus (PCV) have been described, the non-pathogenic PCV1 and the pathogenic PCV2 in pigs. PCV-ORF1 encodes Rep and Rep' proteins which have identical N-terminal sequence (RepN) within each PCV strain, but RepN has only 88% similarity between PCV1 and PCV2. Purified RepN...
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PMID: 20152863
PDF is available here.
Abstract
Light Upon eXtension real-time PCR (LUX real-time PCR) assay was developed for the detection of porcine circovirus type 2 (PCV2). The primers flanking a 114 bp fragment were selected from ORF1. The optimized assay could detect 20 viral copies of pBluescript SK+ plasmid containing inserted PCV2 DNA....
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PMID: 20138916
PDF is available here.
Suzhen Yang,
Jifei Yang,
Gaiping Zhang,
Xuannian Wang,
Songlin Qiao,
Dong Zhao,
Yubao Zhi,
Xuewu Li,
Guangxu Xing,
Jun Luo,
Jianming Fan and
Dengke Bao
Abstract
An immunochromatographic strip was developed for the serological detection of type O foot-and-mouth disease (FMD) in swine. In the strip, the expressed protein of VP1, the main protective antigen of FMDV, labeled with colloidal gold was used as the detector, the staphylococcal protein A (SPA) and sw...
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PMID: 20100515
PDF is available here.
Abstract
Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine group A rotavirus (PRV-A) are major viruses causing enteric diseases of piglets. A multiplex nested reverse transcription polymerase chain reaction (multiplex nested RT-PCR) was developed for the detectio...
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PMID: 20170679
PDF is available here.
Abstract
Three 4-week-old Yorkshire-Hampshire cross piglets from a litter of 9 (7 liveborn) developed convulsions the day of weaning. They were subsequently obtunded, ataxic, and hypermetric and had intention tremors. An affected male pig was presented live for necropsy on day 5 postweaning. This animal was...
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PMID: 20351358
PDF is available here.
Abstract
We evaluated the effects of Salmonella enterica serovar Typhimurium (Typhimurium) and serovar Choleraesuis (Choleraesuis) on critical regulatory components of growth in young swine. Weaned pigs were housed 2 per pen with ad libitum access to feed and water in a 14-d experiment. Pigs were then repeat...
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PMID: 20081084
PDF is available here.
Abstract
Lawsonia intracellularis is endemic in pig herds in Australia and cost-effective strategies to reduce reliance on antibiotics, such as vaccination and/or all-in/all-out pig flow coupled with cleaning and disinfection of pens, are warranted....
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PMID: 20529028
PDF is available here.
Abstract
A short duration of farrowing is important for piglet survival as a delay can increase the number of stillborn. Many factors may affect the duration of farrowing, including breed, age of the sow, length of gestation, number of piglets born, housing (CRATE vs. PEN), body condition of the sow and stat...
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PMID: 20053511
PDF is available here.
Abstract
The alcoholic extract of Lysimachia ramosa Wall (Primulaceae) was tested in vitro against helminth parasites, Fasciolopsis buski and Ascaris suum, from porcine hosts and Raillietina echinobothrida from domestic fowl. The live adult parasites, collected from a freshly autopsied host, were exposed to...
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PMID: 20138704
PDF is available here.
Abstract
The genus pestivirus contains four recognized species: classical swine fever virus, border disease virus, bovine viral diarrhoea virus types 1 and 2. All are economically important and globally distributed but classical swine fever is the most serious, concerning losses and control measures. It affe...
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PMID: 19854591
PDF is available here.
Abstract
We tested these assays on swine and show that vaccinated animals had FMDV-specific IgM and IgG but no IgA in either serum or saliva. After the infection, both assays detected FMDV-specific IgM, IgG, and IgA in serum. Notably, serum IgA was more readily detected using the ACA-ELISA, whereas IgA was n...
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PMID: 20107003
PDF is available here.