Abstract
Cilium and flagellum beating are important in reproduction and defects in their motion are associated with ectopic pregnancy and infertility. Adrenomedullin (ADM) is a polypeptide present in the reproductive system. This report demonstrates a novel action of ADM in enhancing the flagellar/ciliary be...
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PMID: 20444935
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Abstract
Rhabdias casiraghii n. sp. and R. kibiraensis n. sp. are described from Trioceros ellioti from Burundi and co-infection was observed in one of the host specimens. Distinctive characters between these species are, among others, the mouth and buccal capsule in front view, both of which are round in th...
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PMID: 20597435
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Abstract
This study assessed the effect of dissolved organic matter on the copper (Cu) bioavailability to mammalian cells, porcine oviductal epithelial cells (POEC), in order to imply its effect onto humans. Cu toxicity was investigated in the presence of with and without fulvic acid (FA). Dissociation and e...
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PMID: 19727569
PDF is available here.
Abstract
Modern society continuously exposes the population to electromagnetic radiation, the effects of which on human health, in particular reproduction, are still unknown. The aim of this research was to assess the effect of acute (1h) exposure of boar spermatozoa to a 50 Hz extremely low frequency electr...
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PMID: 20176397
PDF is available here.
Abstract
We hypothesized that during host invasion, the replicating IBV population further changes as it confronts the distinct environments of different tissues, leading to selection of the most fit population. We inoculated 15-day-old chickens with 10(4) 50% embryo infective doses of an Ark-type IBV commer...
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PMID: 20608523
PDF is available here.
Abstract
These results suggest that the status of the reproductive tract at the time of infection may impact recovery of culture-positive tissue and contribute to variance. It is suggested that Salmonella Enteritidis cultures that vary in subpopulation composition have subtle differences in colonization of r...
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PMID: 20608530
PDF is available here.
Abstract
The oviductal gland is an exclusive structure of cartilaginous fishes that produces the egg jelly, forms the tertiary egg envelopes and stores sperm. The biological importance of this structure is related to the special features of the reproductive strategy of the group and to its phylogeny, conside...
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PMID: 20552146
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Abstract
The presence of the mu-opioid receptor (MOR) was investigated in the mare oviduct during oestrus and anoestrus, by means of immunoblotting and immunohistochemistry. Immunoblotting analysis showed that the MOR protein is expressed as 65, 50 and 30 kDa forms in the infundibulum and ampulla both in oes...
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PMID: 20036785
PDF is available here.
Abstract
The objective of this study was to estimate the sensitivity of three different sampling/testing methodologies for the detection of Salmonella Enteritidis in commercial egg-laying flocks relative to the within-flock prevalence. The following methods were compared on 21 farms: (1) The European Union (...
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PMID: 19698210
PDF is available here.
Abstract
Mycoplasma genitalium is an emerging sexually transmitted infection and in women is associated with notable reproductive tract syndromes such as cervicitis, pelvic inflammatory disease, and infertility. Investigations into the causal relationships of M. genitalium infections and clinical disease hav...
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PMID: 19995897
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Abstract
We also observed substantial staining for pSMADs in intact fully-grown oocytes from antral follicles. In granulosa cells, immunostaining for both pSMAD2/3 and pSMAD1/5/9 was apparent in preantral granulosa cells from primordial to secondary follicles. In antral follicles, immunostaining for pSMADs b...
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PMID: 20176141
PDF is available here.
Abstract
This study reports that the cytopathic effect of Trichomonas vaginalis, an important human parasite of the urogenital tract, occurs due to mechanical stress and subsequent phagocytosis of the necrotic cells. The investigation was done using a primary culture of bovine oviduct epithelial cells (BOECs...
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PMID: 19723359
PDF is available here.
Abstract
We successfully generated an alpha34 monoclonal antibody (mAb) which neutralized sperm motility-initiating activity in the egg-jelly of the newt, Cynops pyrrhogaster, in a dose-dependent manner. The alpha34 mAb recognized an unglycosylated 34 kDa protein in the outermost of the six layers that compr...
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PMID: 20209432
PDF is available here.
Abstract
In placental (eutherian) mammals, a number of important events take place within the oviduct including the pre-fertilisation maturation of gametes (including sperm storage), sperm-egg interactions, egg activation and early embryonic development. Many of these events involve interactions of glycoconj...
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PMID: 19924648
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Abstract
We determined if addition of caffeine and CaCl(2) to semen extender improves the fertility of frozen-thawed boar semen. In experiment 1, gilts were cervically inseminated twice with frozen-thawed boar spermatozoa (25 x 10(8) cells per dose) suspended in Modena solution (n=7) or modified Beltsville T...
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PMID: 19734696
PDF is available here.
Abstract
SummaryBufo arenarum oocytes are oviposited surrounded by jelly coats, one component of the extracellular matrix required for fertilization. The secretion, released to the oviductal lumen, was analysed by SDS-PAGE. The coomassie blue staining evidenced an electrophoretic pattern with molecules rangi...
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PMID: 19500442
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Abstract
We investigated the effects of genistein supplementation on the development of fibroid tumors in the oviduct, serum oxidative stress markers [malondialdehyde (MDA), 8-isoprostane, 4-hydroxyalkenal (HAE), 8-hydroxy-2' -deoxyguanosine (8-OHdG) levels], soy isoflavone levels, and tissue biomarkers [Con...
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PMID: 20155619
PDF is available here.
Abstract
These results provide enhanced insights into the complex interplay between local host innate immune system and bacterial virulence factors....
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PMID: 19848079
PDF is available here.
Abstract
With an aim to isolate, culture and characterize goat embryonic stem cell-like cells derived from in vitro fertilized goat blastocysts, slaughterhouse derived goat oocytes were in vitro matured in maturation medium in 5% CO2 air at 38.5 degrees C. Matured oocytes were fertilized in vitro with fresh...
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PMID: 19775069
PDF is available here.
Abstract
I and wheatgerm agglutinin); however, Bandeiraea simplicifolia agglutinin I reacted with both cell types. These staining patterns were different in the progesterone-treated animals. Differences in glycan distribution in the oviductal secretions were revealed by lectin blotting. Compared with the sea...
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PMID: 19340461
PDF is available here.
Abstract
We can not confirm a direct association between high P4 concentrations and embryo survival during early stages, although P4 may influence early embryo development through different mechanisms mediated by the type of cells present....
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PMID: 18374525
PDF is available here.
Abstract
To clarify breeding failure in avian species caused by the estrogenicity of chemicals, alterations in the reproductive systems of Japanese quail exposed in ovo to a xenoestrogen were investigated. An injection of diethylstilbestrol (DES) into the yolk before incubation decreased, after sexual matura...
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PMID: 18597071
PDF is available here.
Abstract
Based on the expression levels in uninfected COEC, AvBDs can be loosely grouped into three categories with AvBD4-5 and AvBD9-12 being constitutively expressed at high levels; AvBD1, AvBD3, and AvBD13-14 at moderate levels; and AvBD2 and AvBD6-8 at minimal levels. Infection with the wild type SE stra...
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PMID: 19642979
PDF is available here.
Abstract
Sperm hyperactivation is characterised by high-amplitude, asymmetrical flagellar bending and is required to penetrate the oocyte zona pellucida. It was proposed that hyperactivation also enables spermatozoa to reach the oocyte by assisting escape from the oviductal sperm reservoir. To test this hypo...
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PMID: 19210926
PDF is available here.
Abstract
A different MSSCP pattern was shown which indicates that mutation is located in this region. The samples of different conformers were sequenced and the A --> G transitions was identified in two positions -617 and -608. Restriction analysis of the DNA was performed, but unfortunately none of the know...
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PMID: 20010486
PDF is available here.
Abstract
We noted different kids of ovarian cysts and structural changes in ovarian cortex like decreasing numbers of ovarian follicules of all generations, increasing of follicular atresia. We compared the state of oviduct and uterus mucosa in case of polycystic ovaries with the histological changes in thes...
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PMID: 19419946
PDF is available here.
Abstract
A unique property of the avian oviduct is to store sperm for a prolonged period. The sperm storage tubules (SST) are located in the utero-vaginal junction of the oviduct, where sperm can be stored and survived for a few weeks after insemination or natural mating. The immune system in the oviduct is...
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PMID: 19032608
PDF is available here.
Abstract
We conclude that antibodies to OPN, L-PGDS and BSA react with oocytes incubated with ODF and inhibit sperm binding, fertilization and embryonic development in vitro, suggesting a potential role of these proteins in these events....
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PMID: 18484958
PDF is available here.
Abstract
These results suggest that a fine-tuned PGE(2) signal transduction pathway may support fertilization, early embryonic development and gamete transport in the bovine oviduct....
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PMID: 19261969
PDF is available here.
Abstract
The ribonuclease III endonuclease, Dicer1 (also known as Dicer), is essential for the synthesis of the 19-25 nucleotide noncoding RNAs known as micro-RNAs (miRNAs). These miRNAs associate with the RNA-induced silencing complex to regulate gene expression posttranscriptionally by base pairing with 3'...
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PMID: 18703631
PDF is available here.
Abstract
We previously showed that post-hatch oral estrogen and xenoestrogen exposure disrupts reproduction by increasing eggshell breakage in females and decreasing fertility in males. Here we show that post-hatch exposure to estradiol benzoate (by oral gavage on days 5 to 11) at a 100 nmol EB per g body ma...
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PMID: 18671991
PDF is available here.
Abstract
We hypothesize that zona pellucida (ZP) changes during transit of the oocyte along the oviductal ampulla modulate the interaction with spermatozoa, contributing to the regulation of polyspermy. We report here that periovulatory oviductal fluid (OF) from sows and heifers increases (both, con- and het...
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PMID: 18838686
PDF is available here.
Abstract
We conditionally inactivated Dicer1 in the mesenchyme of the developing Müllerian ducts and postnatally in ovarian granulosa cells and mesenchyme-derived cells of the oviducts and uterus. Deletion of Dicer in these cell types results in female sterility and multiple reproductive defects including d...
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PMID: 18687735
PDF is available here.
Abstract
We inferred that the expression of Cu,ZnSOD was influenced by the hormonal milieu. Furthermore, variations among oviduct sections in protein expression profile of SODs suggested they have an important role in preserving and capacitating sperm....
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PMID: 18572235
PDF is available here.
Abstract
We used microarray, proteomic, and comparative analyses to characterize gene expression in oviducts of mated and unmated Drosophila females. The transition from non-egg laying to egg laying elicits a distinct molecular profile in the oviduct. Immune-related transcripts and proteins involved in muscl...
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PMID: 18725632
PDF is available here.
Abstract
The morphology and histology of male and female reproductive systems were examined in Scoloplax distolothrix. Internal insemination was documented in this species by the presence of sperm within the ovaries. Mature males and females have elongated genital papillae, exhibiting a tubular shape in male...
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PMID: 18553423
PDF is available here.
Abstract
This article reports 5 cases of oviduct adenocarcinoma in adult captive snakes from Smithsonian's National Zoological Park. This neoplasm was found in 1 of each of the following species: emerald tree boa (Corallus caninus), Amazonian tree boa (Corallus enydris enydris), Burmese rock python (Python m...
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PMID: 18725476
PDF is available here.
Abstract
We identified for the first time the precursor of chicken matrix metalloprotease 2 (pro-MMP-2) as a complex with TIMP-2 (tissue inhibitor of metalloproteinases) in egg white and yolk. Real-time polymerase chain reaction confirmed that MMP-2 and its inhibitors TIMP-2 and TIMP-3 were expressed all alo...
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PMID: 18620399
PDF is available here.
Wai K Leung,
Kai-chun Wu,
Christine Y P Wong,
Alfred S L Cheng,
Arthur K K Ching,
Anthony W H Chan,
Wilson W S Chong,
Minnie Y Y Go,
Jun Yu,
Ka-Fai To,
Xin Wang,
Y L Chui,
D M Fan and
Joseph J Y Sung
Abstract
We determined the effects of high salt on gastric chemical carcinogenesis in COX-2 transgenic (TG) mice. COX-2 TG mice were developed in C57/BL6 strain using the full-length human cox-2 complementary DNA construct. Six-week-old COX-2 TG and wild-type (WT) littermates were randomly allocated to recei...
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PMID: 18611916
PDF is available here.
Abstract
The annual oviductal cycle of the Cottonmouth, Agkistrodon piscivorus, is described using electron microscopy. This is only the second such study on a snake and the first on a viperid species. Specimens were collected in reproductive and nonreproductive condition throughout the year and five ultrast...
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PMID: 18488993
PDF is available here.
Abstract
Salmonella enterica serotype Enteritidis is the predominant serotype associated with egg-borne salmonellosis in humans. Apparently this serotype possesses particular characteristics that increase its chance to contaminate eggs. To identify these characteristics, two Salmonella serotype Enteritidis s...
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PMID: 18622856
PDF is available here.
Abstract
We have examined the expression and distribution of ASIC2 in rat ciliated cells (trachea and oviduct) and stereociliated cells (epididymis, Corti organ, and ampullary crest) by immunohistochemistry and transmission electron microscopy (TEM). Immunohistochemistry revealed that ASIC2 was expressed in...
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PMID: 18560896
PDF is available here.
Abstract
We validated methods of enhancing the transcriptional activity of weak promoters using a Cre-loxP system in vitro and in vivo. We constructed a tester vector, pCTL, which carries a strong systemic cytomegalovirus enhancer/chicken beta-actin promoter (CAG), loxP-flanked CAT, and firefly luciferase (l...
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PMID: 18163444
PDF is available here.
Abstract
Primordial, pre-vitellogenic and vitellogenic follicles were present in the ovary of the immature ostrich. The oocytes of these follicles were composed of a nucleus surrounded by ooplasm. Central, intermediate and cortical regions formed the ooplasm. The organelles present in these ooplasmic regions...
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PMID: 18221500
PDF is available here.
Abstract
The morphological characteristics of the oviduct of 12 sexually mature rheas (Rhea americana) were studied. Only the left oviduct is developed as a long tube with a length of 122 +/- 23.1 cm, and is subdivided into infundibulum (15.2 +/- 4.0 cm), magnum (63.3 +/- 9.4 cm), isthmus (5.6 +/- 3.1 cm), u...
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PMID: 18070241
PDF is available here.
Abstract
We had previously observed the upregulation of these cytokines expression in an earlier study (molting by feed withdrawal). However, the pattern and the level of expression were different among these two methods. These findings indicate that cytokines might be a common mediator of tissue regression...
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PMID: 18351392
PDF is available here.
Abstract
We used two independently derived mouse models of CF. Both of these models displayed decreased fertility characterized by a reduction in litter number and litter size. Our findings suggest that much of the reduced fertility in these mice originates from decreased fertilization due to inadequate sper...
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PMID: 18325992
PDF is available here.
Abstract
These results provide strong evidence that purinergic signaling constitutes a key mechanism of regulating chloride secretion and thus fluid formation in the bovine oviduct....
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PMID: 18322271
PDF is available here.
Abstract
We study the microanatomy of the oviduct and the oviducal gland of Sympterygia acuta. The results show a very folded oviduct with mucous and ciliated cells. The oviducal gland depicts the same zonation as other batoids, showing the typical four regions (club, papillary, baffle and terminal). The epi...
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PMID: 18660964
PDF is available here.
Abstract
We examined the transmission of mtDNA from NT cows to G(1) offspring. Eleven NT founder cows were produced by fusion of enucleated oocytes (Holstein/Japanese Black) with Jersey/ Holstein oviduct epithelial cells, or Holstein/Japanese Black cumulus cells. Transmission of mtDNA was analyzed by PCR med...
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PMID: 18033682
PDF is available here.
Abstract
The ultrastructural changes occurring in the fully functional oviduct of Isa Brown laying hens were studied during various stages of the laying cycle. Hens were killed at different positions of the egg in the oviduct. The oviduct was lined by ciliated and non-ciliated cells (also referred to as gran...
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PMID: 18236079
PDF is available here.
Abstract
These results suggest that all 11 types of AvbetaD are expressed in the hen oviduct and at least 5 of them in the vagina show increased expression in response to LPS....
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PMID: 18420991
PDF is available here.
Abstract
We reported the identification of the first vertebrate sperm chemoattractant, allurin, in the frog Xenopus laevis (Xl) and demonstrated that it was a member of the CRISP family of proteins. Here we report identification, purification, and characterization of Xenopus tropicalis (Xt) allurin, a homolo...
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PMID: 18342304
PDF is available here.
Abstract
We show that both high viscous loading and hypotonicity stimuli in native ciliated epithelial cells use PLA(2)-EET as the primary pathway to activate TRPV4. Under conditions of low PLA(2) activation, both also use extracellular ATP-mediated activation of phospholipase C (PLC)-inositol trisphosphate...
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PMID: 18378772
PDF is available here.
Abstract
Preimplantation development of canine embryos is not well understood. To understand the timing of preattachment embryogenesis relative to the luteinizing hormone (LH) surge, early embryonic development was examined in Labrador Retrievers after artificial insemination. The embryos migrated from the o...
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PMID: 18202549
PDF is available here.
Abstract
This study was conducted to investigate the developmental capacity of domestic cat-bovine reconstructed embryos via interspecies somatic cell nuclear transfer (iSCNT) and to observe the mitochondrial DNA (mtDNA) content of the iSCNT embryos. The iSCNT embryos were generated using mixed-breed domesti...
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PMID: 18239350
PDF is available here.
Abstract
We evaluate the effect of SBG over boar sperm. We show that the presence of SBG produces alterations of the acrosome morphology of sperm only when they are incubated in capacitating conditions. SBG binds to the periacrosomal region of sperm undergoing capacitation. Its presence induces an increase o...
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PMID: 17786920
PDF is available here.
Abstract
Previous studies from our laboratory have reported empirical associations between bovine seminal plasma protein(s) (BSP) A1/A2 and 30 kDa and osteopontin (OPN) in accessory sex gland fluid and bull fertility. These BSP and OPN are believed to bind to sperm at ejaculation and to remain bound until sp...
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PMID: 18207674
PDF is available here.
Abstract
A case of oviductal volvulus, a rare disorder with oviductal cyst as a possible aetiology, is described in an 11-month-old Nera black chicken (Gallus gallus domesticus). The condition was observed during routine postmortem examination of 550 carcasses from a flock of 16,100 birds between September 2...
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PMID: 18393090
PDF is available here.