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Drosophila melanogaster (0):
Recent article
Drosophila melanogaster
Abstract
We isolated a novel Drosophila male sterile mutant, garmcho (garm). Positional cloning indicated that garm encodes a subunit of the Drosophila THO complex, THOC5. Flies lacking THOC5 showed a meiotic arrest phenotype with severe nucleolar disruption in primary spermatocytes. A functional GFP-tagged...
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PMID: 21828100
PDF is available here.
PDF is available here.
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Abstract
We use Drosophila to explore the as yet unknown mechanisms regulating this privileged tissue growth. As in mammals, we observe in Drosophila that the CNS is more highly spared than other tissues during nutrient restriction (NR). We demonstrate that anaplastic lymphoma kinase (Alk) efficiently protec...
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PMID: 21816278
PDF is available here.
PDF is available here.
Abstract
We identified a volatile, low molecular weight molecule, 2-amino acetophenone (2-AA), produced by the opportunistic human pathogen Pseudomonas aeruginosa that reduces bacterial virulence in vivo in flies and in an acute mouse infection model. 2-AA modulates the activity of the virulence regulator Mv...
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PMID: 21829370
PDF is available here.
PDF is available here.
Genetika (Moskva) (47)8 2011
Abstract
We studied the Grp gene (CG4680, Gag related protein) expression at the transcriptional level. It was found that at the embryonic and larval stages of D. melanogaster development the Grp expression proceeds at a low level, but it significantly increases at the adult stage. Adult individuals display...
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PMID: 21954611
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We present a basic method for the purification of the endogenous Par-6/aPKC protein complex, which plays a central role in orchestrating asymmetric cell divisions in the developing nervous system of Drosophila. The procedure can be subdivided into the following steps: acquisition of sufficient start...
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PMID: 18641959
PDF is available here.
PDF is available here.
Development (138)8 2011
Abstract
We examine the possibility that a recently derived testis-expressed WISPY paralog, GLD2, plays a similar role in the Drosophila male germline. We show that knockdown of Gld2 transcripts causes male sterility, as GLD2-deficient males do not produce mature sperm. Spermatogenesis up to and including me...
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PMID: 21427144
PDF is available here.
PDF is available here.
Abstract
Abstract
We show that Zuc localizes to mitochondria and has MitoPLD-like activity. Conversely, MitoPLD(-/-) mice exhibit the meiotic arrest, DNA damage, and male sterility characteristic of mice lacking piRNAs. The primary function of MitoPLD seems to be the generation of mitochondrial-surface PA. This PA in...
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PMID: 21397848
PDF is available here.
PDF is available here.
Abstract
We quantified Dpp concentration and signaling levels during wing disc growth. Both Dpp concentration and signaling gradients scale with tissue size during development. On average, cells divide when Dpp signaling levels have increased by 50%. Our observations are consistent with a growth control mech...
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PMID: 21385708
PDF is available here.
PDF is available here.
Abstract
We describe a system capable of tracking the three-dimensional position and body orientation of animals such as flies and birds. The system operates with less than 40 ms latency and can track multiple animals simultaneously. To achieve these results, a multi-target tracking algorithm was developed b...
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PMID: 20630879
PDF is available here.
PDF is available here.
Abstract
We report mutants of a novel Drosophila gene twenty-four (tyf) that show weak behavioural rhythms. Weak rhythms are accompanied by marked reductions in the levels of the clock protein Period (PER) as well as more modest effects on Timeless (TIM). Nonetheless, PER induction in pacemaker neurons can r...
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PMID: 21331043
PDF is available here.
PDF is available here.
Abstract
We show that Notch is activated in select olfactory receptor neurons (ORNs) in an odorant-specific fashion. This response requires olfactory receptor activity and the Notch ligand Delta. We present evidence that Notch activation depends on synaptic transmission by the ORNs in which the receptors are...
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PMID: 21315258
PDF is available here.
PDF is available here.
Abstract
We determined the cellular substrate for male courtship behavior by quasinatural and artificial stimulation of brain neurons. Activation of fruitless (fru)-expressing neurons via stimulation of thermosensitive dTrpA1 channels induced an entire series of courtship acts in male Drosophila placed alone...
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PMID: 21315260
PDF is available here.
PDF is available here.
Abstract
We have established a tethering system, in which the Gal4 DNA binding domain (DBD) of the Flag:Gal4 DBD:Ote▵LEM fusion protein physically interacts with the Gal4 binding sites upstream of bamP-gfp to artificially recruit the reporter gene gfp to the nuclear membrane. Our data demonstrated that the...
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PMID: 21356524
PDF is available here.
PDF is available here.
Abstract
We have measured the distance d between binding points (motors). The average value of d was determined as a function of motor surface density σ. The measurements agree well with the scaling model of Duke, Holy, and Liebler, which predicts that 〈d〉~σ^{-2/5} if 0.05⩽σ⩽20 μm^{-2} [Phys. R...
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PMID: 21405724
PDF is available here.
PDF is available here.
Abstract
Abstract
Skeletal muscle development, growth and regeneration depend on the ability of progenitor myoblasts to fuse to one another in a series of ordered steps. Whereas the cellular steps leading to the formation of a multinucleated myofiber are conserved in several model organisms, the molecular regulatory f...
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PMID: 21432017
PDF is available here.
PDF is available here.
Science Signaling (4)177 2011
Abstract
The ability to coordinate membrane trafficking events in space and time is of fundamental importance in biology. Polarized trafficking is essential for processes as diverse as cytokinesis, regulated endocytosis, cell motility, and morphogenesis, yet the interface between cellular sig...
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PMID: 21673314
PDF is available here.
PDF is available here.
Abstract
We studied the patterns of small RNAs targeting both strands of suffix in Drosophila ovaries using an RNase protection assay and the analysis of the corresponding RNA sequences from the libraries of total small RNAs. Our results indicate that suffix sense and antisense transcripts are targeted mainl...
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PMID: 21779345
PDF is available here.
PDF is available here.
Abstract
We manipulated levels of Ena, the Drosophila homologue of Mena, in migrating embryonic macrophages (haemocytes). Consistent with data from fibroblasts in vitro, Ena localises to regions of actin dynamics within migrating haemocytes, stimulates lamellipodial dynamics and positively regulates the numb...
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PMID: 21045209
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)52 2010
Abstract
We have determined the human male specific lethal 3 (hMSL3) chromo-barrel domain structure by x-ray crystallography to a resolution of 2.5 Å (r = 0.226, R(free) = 0.270). hMSL3 contains a canonical methyllysine binding pocket made up of residues Tyr-31, Phe-56, Trp-59, and Trp-63. A six-residue in...
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PMID: 20943666
PDF is available here.
PDF is available here.
Abstract
We investigate how cell deformations emerge from the interplay between force generation and cortical force transmission during this remodelling in Drosophila melanogaster. The shrinkage of dorsal-ventral-oriented ('vertical') junctions during this process is known to require planar polarized junctio...
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PMID: 21068726
PDF is available here.
PDF is available here.
Abstract
We report the crystal structures of the precursor p62-E1 heterodimer and of the mature E3-E2-E1 glycoprotein complexes. The resulting atomic models allow the synthesis of a wealth of genetic, biochemical, immunological and electron microscopy data accumulated over the years on alphaviruses in genera...
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PMID: 21124458
PDF is available here.
PDF is available here.
Abstract
We identify a neural circuit responsive to cVA, a pheromone that elicits sexually dimorphic behaviours. We have combined neural tracing using an improved photoactivatable green fluorescent protein (PA-GFP) with electrophysiology, optical imaging and laser-mediated microlesioning to map this circuit...
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PMID: 21124455
PDF is available here.
PDF is available here.
Abstract
We report the structure of an alphavirus spike, crystallized at low pH, representing an intermediate in the fusion process and clarifying the maturation process. The trimer of E2-E1 in the crystal structure is similar to the spikes in the neutral pH virus except that the E2 middle region is disorder...
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PMID: 21124457
PDF is available here.
PDF is available here.
Abstract
We have investigated the effect of insertion of sites of differing affinities, either upstream or within the transcribed gene, on complex binding and transcription upregulation. Insertion of four copies of the 18D11 fragment upstream or at the 3' end of a reporter gene led to strong MSL complex bind...
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PMID: 20977887
PDF is available here.
PDF is available here.
Nature Neuroscience (13)12 2010
Abstract
We found that the same vesicles participate in active and spontaneous release....
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PMID: 21102450
PDF is available here.
PDF is available here.
Abstract
We screened pools of P element-mediated mutants and tested locomotor activity of homozygous flies. Of 1014 P element-mutagenized lines, 638 were homozygous viable. These lines were tested for climbing ability and lifespan. We isolated dGIPC, a Drosophila homolog of GIPC, that produced a 50% prematur...
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PMID: 21029723
PDF is available here.
PDF is available here.
Abstract
We present direct biochemical evidence that Tudor-Aubergine interaction surface is composed of different Tudor domains including those that have not been previously implicated in Aubergine recognition. Furthermore, we show that specific single Tudor domains determine localization of Tudor complex to...
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PMID: 20946872
PDF is available here.
PDF is available here.
Abstract
The human brain contains more than 100 trillion (10(14)) synaptic connections, which form all of its neural circuits. Neuroscientists have long been interested in how this complex synaptic web is weaved during development and remodelled during learning and disease. Recent studies hav...
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PMID: 21068831
PDF is available here.
PDF is available here.
Abstract
We examine how these pathways contribute to visual motion detection by combining genetic block and reconstitution of neural activity in different lamina cell types with whole-cell recordings from downstream motion-sensitive neurons. We find reduced responses to moving gratings if L1 or L2 is blocked...
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PMID: 21068841
PDF is available here.
PDF is available here.
Abstract
Journal of Biological Chemistry (285)45 2010
Abstract
We demonstrate that RNAi to individual pgant genes results in specific reduction in gene expression without affecting the expression of other family members. Cells with reduced expression of individual pgant genes were then examined for changes in viability, morphology, adhesion, and secretion to as...
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PMID: 20807760
PDF is available here.
PDF is available here.
Developmental Biology (347)1 2010
Abstract
We genetically created such neuroblasts, and found that in embryos, they were initially mitotic and then gradually differentiated into neurons, frequently forming a clone of cells homogeneous in temporal identity. By contrast, larval neuroblasts with the same genotype continued to proliferate withou...
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PMID: 20599889
PDF is available here.
PDF is available here.
Anti-Cancer Drugs (21)10 2010
Abstract
We sought to investigate their impact on normal cells by using Drosophila as a model. We investigated the possible involvement of Drosophila homologue of p53 (Dmp53) and a member of the retinoblastoma binding protein 6 family, known as Snama. On account of its molecular features and experimental evi...
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PMID: 20717003
PDF is available here.
PDF is available here.
Abstract
Current Biology (20)20 2010
dDOR is an EcR coactivator that forms a feed-forward loop connecting insulin and ecdysone signaling.
Abstract
We analyze here the in vivo function of DOR by studying flies mutant for Drosophila DOR (dDOR).
We show that dDOR is a novel coactivator of ecdysone receptor (EcR) that is needed during metamorphosis. dDOR binds EcR and is required for maximal EcR transcriptional activity. In the absence of dDOR, f...
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PMID: 20888228
PDF is available here.
PDF is available here.
Abstract
We report that CG10527 is expressed widely in secondary cells in the male accessory glands, in ovarian follicle cells, and in glial cells in the nervous system. Furthermore, CG10527 is expressed abundantly in the corpora allata where JH is synthesized. To understand the physiological functions of CG...
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PMID: 20833126
PDF is available here.
PDF is available here.
Abstract
We use Drosophila melanogaster to identify the Rab11/Sec15 exocyst, which acts at the last step of endocytic recycling, as a novel target of both EF and LF. EF reduces levels of apically localized Rab11 and indirectly blocks vesicle formation by its binding partner and effector Sec15 (Sec15-GFP), wh...
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PMID: 20944747
PDF is available here.
PDF is available here.
Abstract
Abstract
The hypothesis, that structural deterioration of cytochrome c oxidase (CcO) is a causal factor in the age-related decline in mitochondrial respiratory activity and an increase in H₂O₂ generation, was tested in Drosophila melanogaster. CcO activity and the levels of seven different nuclear DNA-en...
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PMID: 20833144
PDF is available here.
PDF is available here.
Tissue & Cell (42)5 2010
Abstract
We investigated the transovarial inheritance of Wolbachia in two phylogenetically distant insects, Drosophila melanogaster and Zyginidia pullula. We detected in both systems bacteriocyte-like cells, densely packed with Wolbachia endosymbionts, at the tip of the ovarioles. Bacteriocytes are cells spe...
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PMID: 20817243
PDF is available here.
PDF is available here.
Abstract
To unveil the mechanism of sleep-improving effect of lyophilized powder of Sini powder on drosophila.
Drosophila melanogaster whose strain were wild Canton S, unmated, were collected daring 12 h after their emergencing, then they were anaesthetized by small flow of CO2 and divided into different tub...
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PMID: 21246833
PDF is available here.
PDF is available here.
Abstract
We sequenced multiple alleles of the eater gene from an African and a North American population of D. melanogaster and found signatures of a partial selective sweep in North America that is localized around the second intron. This pattern is consistent with local adaptation to novel selective pressu...
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PMID: 20444883
PDF is available here.
PDF is available here.
Food and Chemical Toxicology (48)10 2010
Abstract
The study examines the potential genotoxicity of three antiepileptic drugs (phenytoin sodium, pregabalin, gabapentin) using the wing somatic mutation and recombination test (SMART) in Drosophila melanogaster. Trans-heterozygous (two genetic markers mwh and flr) third-instar larvae of D. melanogaster...
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PMID: 20600525
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
The Polycomb (PcG) and Trithorax (TrxG) group proteins are essential for development in all multicellular organisms. Mutations of the PcG and TrxG genes act as early embryonic lethals, while their overexpression correlates with malignancies. Comparative genome analysis showed that PcG and TrxG form...
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PMID: 21254568
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
The Merlin gene of Drosophila is homologous to the human Neurofibromatosis 2 (NF2) gene an important regulator of proliferation and endocytosis of cell receptors. It was earlier shown that the Thr5 residue of the Drosophila Merlin protein was homologous to Ser518 of the human protein (which was alre...
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PMID: 21254560
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
Data supporting the hypothesis about the epigenetic nature of deleterious mutations leading to the death of a part of the progeny are presented. It is assumed that during the life cycle "mutant" variants of formation of structural-functional loop domains occur in chromosomes that normally are correc...
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PMID: 21254558
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
Molecular combing (MC) yields preparations where individual DNA molecules are uniformly stretched and are parallel to each other. Fluorescence in situ hybridization on such preparations allows an exact mapping of DNA sequences, and pulsed inclusion of halogenated deoxyuridine analogs and their detec...
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PMID: 21254567
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
The functional organization of particular chromosome regions is tightly associated with their function in eukaryotic cells. Details of this association are among the most topical problems of modem genetics. The paper characterizes the results of recent research of the specifics of the genetic organi...
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PMID: 21254569
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
We demonstrate that it is transcription of the gene on the X chromosome that is important for MSL targeting, irrespective of gene origin and DNA sequence....
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PMID: 21254570
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
The modern concept of intercalary heterochromatin as polytene chromosome regions exhibiting a number of specific characteristics is formulated. DNA constituting these regions is replicated late in the S period; therefore, some strands of polytene chromosomes are underrepresented; i.e., they are unde...
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PMID: 21254566
PDF is available here.
PDF is available here.
Abstract
We describe an alternative mode of linear chromosome maintenance in which canonical telomeres are superseded by blocks of heterochromatin. We show that in the absence of telomerase, Schizosaccharomyces pombe cells can survive telomere sequence loss by continually amplifying and rearranging heterochr...
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PMID: 20829796
PDF is available here.
PDF is available here.
Neurochemical Research (35)9 2010
Abstract
We found significant diminution in the endogenous levels of oxidative markers in whole body homogenates of flies exposed to Cr (2-10 mM). Cr supplementation resulted in reduced mortality in flies exposed to rotenone (500 microM) and better performance in a negative geotaxis assay. Further Cr (10 mM)...
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PMID: 20514516
PDF is available here.
PDF is available here.
Optics Express (18)18 2010
Abstract
A 3D structured light sheet microscope using a four-faceted symmetric pyramid is presented. The sample is illuminated by the resulting four beam interference field. This approach combines advantages of standing wave and structured illumination microscopy. Examples of micrographs of fluorescently lab...
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PMID: 20940819
PDF is available here.
PDF is available here.
Molecular Cell (39)4 2010
Abstract
We identify the dSTRIPAK PP2A complex as a major regulator of Hpo signaling. dSTRIPAK depletion leads to increased Hpo activatory phosphorylation and repression of Yki target genes in vivo, suggesting this phosphatase complex prevents Hpo activation during development.
Copyright (c)...
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PMID: 20797625
PDF is available here.
PDF is available here.
PLoS Genetics (6)9 2010
Abstract
We assayed the genomic consequences of disregulating the replication licensing mechanisms by RNAi depletion of geminin. We found that not all origins of replication were sensitive to geminin depletion and that heterochromatic sequences were preferentially re-replicated in the absence of licensing me...
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PMID: 20838463
PDF is available here.
PDF is available here.