Abstract
Extremophiles can be defined as organisms that can survive in extreme environments that cannot support mammalian life. They include microorganisms that can tolerate temperature extremes, extremes of pH, salinity, hydrostatic pressure and ionizing radiation, as well as low oxygen tension, desiccation...
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PMID: 20662377
PDF is available here.
Abstract
We focus on three different mechanisms that are employed by a few model organisms, viz. Caldicellulosiruptor saccharolyticus and Thermoanaerobacter tengcongensis, Thermotoga maritima, and Pyrococcus furiosus, to efficiently produce hydrogen. In addition, recent developments to improve hydrogen produ...
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PMID: 20662387
PDF is available here.
Abstract
Domestic ethanol production in the USA relies on starch feedstocks using a first generation bioprocess. Enzymes that contribute to this industry remain of critical value in new and established markets as commodity additives and for in planta production. A transition to non-food feedstocks is both de...
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PMID: 20662388
PDF is available here.
Abstract
Molecular characterization of subsurface microbial communities in the former Homestake gold mine, South Dakota, was carried out by 16S rDNA sequence analysis using a water sample and a weathered soil-like sample. Geochemical analyses indicated that both samples were high in sulphur, rich in nitrogen...
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PMID: 20662386
PDF is available here.
Henry Chan,
Vartan Babayan,
Elya Blyumin,
Charmy Gandhi,
Kunal Hak,
Danielle Harake,
Kris Kumar,
Perry Lee,
Tze T Li,
Hao Yi Liu,
Tony Chung Tung Lo,
Cynthia J Meyer,
Steven Stanford,
Krista S Zamora and
Milton H Saier
Abstract
We analyze P-type ATPases in all major prokaryotic phyla for which complete genome sequence data are available, and we compare the results with those for eukaryotic P-type ATPases. Topological type I (heavy metal) P-type ATPases predominate in prokaryotes (approx. tenfold) while type II ATPases (spe...
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PMID: 20962537
PDF is available here.
Abstract
A key element during the flow of genetic information in living systems is fidelity. The accuracy of DNA replication influences the genome size as well as the rate of genome evolution. The large amount of energy invested in gene expression implies that fidelity plays a major role in fitness. On the o...
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PMID: 20871851
PDF is available here.
Abstract
The archaeal L7Ae and eukaryotic 15.5kD protein homologs are members of the L7Ae/15.5kD protein family that characteristically recognize K-turn motifs found in both archaeal and eukaryotic RNAs. In Archaea, the L7Ae protein uniquely binds the K-loop motif found in box C/D and H/ACA sRNAs, whereas th...
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PMID: 19926724
PDF is available here.
Abstract
We analyzed 4925 tRNA sequences from 102 bacterial and 21 archaeal species. Here, we used a classification and regression tree (CART) methodology, and we found that the degrees of degeneracy or specificity of the RNA codes in both Archaea and Bacteria differ from those of the genetic code. We found...
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PMID: 19952117
PDF is available here.
Abstract
We discuss the recent developments in our understanding of this important class of regulators....
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PMID: 21151646
PDF is available here.
Abstract
Proteins can be acetylated at the alpha-amino group of the N-terminal amino acid (methionine or the penultimate amino acid after methionine removal) or at the epsilon-amino group of internal lysines. In eukaryotes the majority of proteins are N-terminally acetylated, while this is extremely rare in...
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PMID: 20885971
PDF is available here.
Abstract
Phylogenomics of eukaryote supergroups suggest a highly complex last common ancestor of eukaryotes and a key role of mitochondrial endosymbiosis in the origin of eukaryotes.
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PMID: 20441612
PDF is available here.
Abstract
We discuss recent developments in our understanding of the archaeal protein secretion mechanisms, the assembly of macromolecular cell surface structures, and the release of S-layer-coated vesicles from the archaeal membrane....
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PMID: 20671907
PDF is available here.
Abstract
The ability of Prunier to take into account branch support in the process of reconciliation allows a gain in complexity, in comparison to EEEP, and in accuracy in comparison to RIATA-HGT. Prunier's greedy algorithm proposes a single scenario of LGT for a gene family, but its quality always compares...
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PMID: 20550700
PDF is available here.
Abstract
Here we introduce an all-against-all sequence- and three-dimensional (3D) structure-based comparison of a representative set of RNA structures, which have allowed us to quantitatively confirm that: (i) there is a measurable relationship between sequence and structure conservation that weakens for al...
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PMID: 20550657
PDF is available here.
Abstract
Five 16S rDNA sequences with the similarity between 93.2% and 99.2% to each other were obtained from Reticulitermes chinensis Snyder. Phylogenetic analysis showed that the five clones were clustered with Methanobrevibacter clones or isolates obtained from Reticulitermes speratus and Reticulitermes f...
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PMID: 20222453
PDF is available here.
Abstract
We speculate that the unique Cys biosynthetic pathway represents a strategy to increase Cys contents by preventing it from thermal degradation, which may be relevant to the thermal adaptation of methanogenic archaea ancestor....
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PMID: 19583437
PDF is available here.
Abstract
Microbial diversity in Movile Cave (Romania) was studied using bacterial and archaeal 16S rRNA gene sequence and functional gene analyses, including ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), soxB (sulfate thioesterase/thiohydrolase) and amoA (ammonia monooxygenase). Sulfur oxidizers...
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PMID: 19474813
PDF is available here.
Abstract
The prokaryotic diversity associated with organic household waste (OHW), leachate (start-up inoculum), and mesophilic anaerobic digestion processes in the degradation of OHW for 44 and 90 days was investigated using a culture-independent approach. Bacterial and archaeal 16S rRNA and mcrA gene clone...
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PMID: 19551378
PDF is available here.
Abstract
Isolation and screening from sea water and sediments, and the optimization of electron donor and inorganic carbon source structure were performed for obtaining microbial flora with high efficient inorganic carbon fixation without the light and hydrogen. In addition, the structure of the microbial fl...
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PMID: 19799314
PDF is available here.
Abstract
Phylogenetic analysis revealed archaea in the soil of Mila Mountain including the Crenarchaeota (71.7%) and unclassified-Archaea (28.3%) phyla. All the Crenarchaeota belong to the Thermoprotei. Phylogenetic analysis revealed AOA in the alp prairie soil of Mila Mountain belonged to the kingdom Crenar...
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PMID: 19835159
PDF is available here.
Abstract
Voltage-dependent K(+) (Kv) channels form the basis of the excitability of nerves and muscles. KvAP is a well-characterized archeal Kv channel that has been widely used to investigate many aspects of Kv channel biochemistry, biophysics, and structure. In this study, a minimal kinetic gating model fo...
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PMID: 19481093
PDF is available here.
Abstract
The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and hi...
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PMID: 19590870
PDF is available here.
Abstract
MP and CP-DNA were always present at lower levels in CC than in IF (p < 0.001) and were correlated with each other only in CC. Electron microscopy revealed Mycoplasma, Chlamydia and two types of archaeal-like bodies. One had electron dense lipid content (EDL) and was mainly present in IF. The other...
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PMID: 19753475
PDF is available here.