Abstract
Macrolide antibiotics have been used to treat acute and chronic inflammation in the respiratory tract based on their antibacterial activity. Now it is well known that macrolides down-regulate damaging prolonged inflammation as well as increase mucous clearance and decrease bacterial...
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PMID: 21991841
PDF is available here.
Abstract
Silver nanoparticle studded scaffolds were prepared by exploiting the Ag(+) ion reducing activity of sophorolipids--a class of 'glycolipids' that cap the ensuing nanoparticles as well. To achieve this, the porous polyethylene scaffolds are subjected to N(2) + H(2) plasma treatment, i...
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PMID: 21643585
PDF is available here.
Abstract
Our results confirm the importance of pH and related geochemical factors in fine-tuning acidophilic microbial community structure and function at the species and strain level, and demonstrate the broad utility of proteomics in laboratory community studies....
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PMID: 21228889
PDF is available here.
Abstract
Indoor air quality has become a critical issue because people spend most of their time in the indoor environment. The factors that influence indoor air quality are very important to environmental sanitation and air quality improvement. This study focuses on monitoring air quality, co...
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PMID: 21850827
PDF is available here.
Abstract
We do not have evidence of SIBO being absent before IBS symptoms, and present after IBS emerges. (3) There is not a dose-response relationship between small intestinal microbiota and IBS symptoms. (4) The relationship between SIBO and IBS is highly inconsistent among studies. (5) Many effective IBS...
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PMID: 21397724
PDF is available here.
Abstract
To examine the yield and resistance profile of pathogens in chronic rhinosinusitis (CRS) patients receiving culture-directed management and to pay particular attention to the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in this population.
Retrospe...
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PMID: 21518646
PDF is available here.
Abstract
We describe the sediment metagenome from the oligotrophic Brazos-Trinity Basin (Site 1320) and show similarities and differences with the dataset from the Pacific Peru Margin (Site 1229) and other pyrosequenced datasets. The microbial community found at Integrated Ocean Drilling Program Site 1320 li...
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PMID: 21209666
PDF is available here.
Abstract
Cocoa, especially the water-insoluble cocoa fraction (WICF), is a rich source of polyphenols. In this study, sequential in vitro digestion of the WICF with gastrointestinal enzymes as well as its bacterial fermentation in a human colonic model system were carried out to investigate b...
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PMID: 21294250
PDF is available here.
Abstract
We applied ecological statistical tools to such culture-independent data, in a novel manner, to partition taxa within the metacommunity into core and satellite species. Sputa and clinical data were obtained from 14 clinically stable adult CF patients. Fourteen rRNA gene libraries were constructed wi...
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PMID: 21151003
PDF is available here.
Abstract
A bio-nanofilm consisting of a tetrad nanomaterial (nanotubes, nanoparticles, DNA, polymer) was fabricated utilizing in situ reduction and noncovalent interactions and it displayed effective antibacterial activity and biocompatibility. This bio-nanofilm was composed of homogenous sil...
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PMID: 21336791
PDF is available here.
Abstract
We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replicatio...
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PMID: 21327279
PDF is available here.
Abstract
The purpose of this study was to prepare a temperature-sensitive gel containing silver nanoparticles and to investigate its anti-bacterial properties in vitro. The aqueous gel was prepared using Pluronic F127 (18-22%) and Pluronic F68 (3-9%) in a cold method to obtain a proper gelati...
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PMID: 21612154
PDF is available here.
Abstract
The effect of calcium concentration on the biofilm structure, microbiology, and treatment performance was evaluated in a moving-bed biofilm reactor. Three experiments were conducted in replicate laboratory-scale reactors to determine if wastewater calcium is an important variable for the design and o...
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PMID: 21466070
PDF is available here.
Abstract
Caspase 1 activation by inflammasome complexes in response to pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) induces the maturation and secretion of the pro-inflammatory cytokines interleukin-1β (IL-1β) and IL-18. Recent reports have...
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PMID: 21350580
PDF is available here.
Abstract
We focus on 'crosstalk manipulation' - the microbial strategies that instigate, subvert or disrupt the molecular signalling crosstalk between receptors of the innate immune system. This proactive interference undermines host defences and contributes to microbial adaptive fitness and persistent infec...
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PMID: 21350579
PDF is available here.
Abstract
To avoid the inhibition of methane production by ammonia that occurs during the degradation of garbage, anaerobic digestion with prior ammonia production and subsequent stripping was investigated. In the ammonia production phase, the maximum ammonia concentration was approximately 28...
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PMID: 21094085
PDF is available here.
Abstract
We compared children living on farms with those in a reference group with respect to the prevalence of asthma and atopy and to the diversity of microbial exposure. In one study--PARSIFAL (Prevention of Allergy-Risk Factors for Sensitization in Children Related to Farming and Anthroposophic Lifestyle...
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PMID: 21345099
PDF is available here.
Alan W Walker,
Jennifer Ince,
Sylvia H Duncan,
Lucy M Webster,
Grietje Holtrop,
Xiaolei Ze,
David Brown,
Mark D Stares,
Paul Scott,
Aurore Bergerat,
Petra Louis,
Freda McIntosh,
Alexandra M Johnstone,
Gerald E Lobley,
Julian Parkhill and
Harry J Flint
Abstract
We examined the influence of precisely controlled diets in 14 overweight men. Volunteers were provided successively with a control diet, diets high in resistant starch (RS) or non-starch polysaccharides (NSPs) and a reduced carbohydrate weight loss (WL) diet, over 10 weeks. Analysis of 16S rRNA sequ...
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PMID: 20686513
PDF is available here.
Abstract
To review the evidence for the presence of bacterial biofilms in chronic rhinosinusitis (CRS) and mechanisms by which they may contribute to the chronic inflammation characteristic of this disease. Lastly, to provide an overview of the current and potential future treatments for bact...
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PMID: 21150431
PDF is available here.
Abstract
We produced metagenomic data by direct 454 pyrosequencing from bathypelagic plankton (1000 m depth) and bottom sediment of the Sea of Marmara, the gateway between the Eastern Mediterranean and the Black Seas. Data from small subunit ribosomal RNA (SSU rRNA) gene libraries and direct pyrosequencin...
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PMID: 20668488
PDF is available here.
Abstract
We describe here the role of muramidases present in clones of metagenomic DNA that result in cell aggregation and biofilm formation by Escherichia coli. The metagenomic clones were obtained from uncultured Lachnospiraceae-affiliated bacteria resident in the foregut microbiome of the Tammar wallaby....
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PMID: 20668486
PDF is available here.
Abstract
Many recent studies have revealed exquisite subcellular localization of proteins, DNA, and other molecules within bacterial cells, giving credence to the concept of prokaryotic anatomy. Common sites for localized components are the poles of rod-shaped cells, which are dynamically mod...
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PMID: 21095111
PDF is available here.
Abstract
Signal transduction systems and ABC transporters often contribute jointly to adaptive bacterial responses to environmental changes. In Bacillus subtilis, three such pairs are involved in responses to antibiotics: BceRSAB, YvcPQRS and YxdJKLM. They are characterized by a histidine kinase belonging to...
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PMID: 21283517
PDF is available here.
Abstract
We aimed to study the effects of gliadin and various intestinal bacterial strains on mucosal barrier integrity, gliadin translocation, and cytokine production.
Changes in gut mucosa were assessed in the intestinal loops of inbred Wistar-AVN rats that were reared under germ-free condi...
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PMID: 21249146
PDF is available here.
Sandrine P Claus,
Sandrine L Ellero,
Bernard Berger,
Lutz Krause,
Anne Bruttin,
Jérôme Molina,
Alain Paris,
Elizabeth J Want,
Isabelle de Waziers,
Olivier Cloarec,
Selena E Richards,
Yulan Wang,
Marc-Emmanuel Dumas,
Alastair Ross,
Serge Rezzi,
Sunil Kochhar,
Peter Van Bladeren,
John C Lindon,
Elaine Holmes and
Jeremy K Nicholson
Abstract
We have probed the systemic metabolic adaptation to gut colonization for 20 days following exposure of axenic mice (n = 35) to a typical environmental microbial background using high-resolution (1)H nuclear magnetic resonance (NMR) spectroscopy to analyze urine, plasma, liver, kidney, and colon (5 t...
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PMID: 21363910
PDF is available here.
Abstract
We intentionally contaminated grapefruit juice to determine whether pathogen bacteria could enter VNBC state. In fact, grapefruit juice contains natural antimicrobial compounds, has an average pH of about 3 and low content in carbohydrates. Such characteristics make grapefruit juice a harsh environm...
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PMID: 20932087
PDF is available here.
Abstract
We briefly review and compare the major srRNA pathways, and argue that the impact of srRNA is always at the system level. We demonstrate how a simple mathematical model can ease the discussion of governing principles. To demonstrate these points we review a few examples from bacteria and animals....
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PMID: 21266101
PDF is available here.
Abstract
We and others have shown in mouse models that colonic bacteria also influence the development of extraintestinal cancers including hepatocellular and mammary carcinomas. Microbes such as Helicobacter hepaticus invoke a proinflammatory microenvironment in the lower bowel that may extend to distant or...
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PMID: 21637019
PDF is available here.
Abstract
We generated and analyzed phenotypes associated with a complete loss-of-function allele of Toll-9. Our results suggest that Toll-9 is neither required to maintain a basal anti-microbial response nor to mount an efficient immune response to bacterial infection....
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PMID: 21386906
PDF is available here.
Abstract
The contamination, survival, and possible foodborne disease outbreaks are major issues confronting the food industry. However, from a microbial perspective, any food whether natural or processed is just another environmental niche that is available for colonization. Quorum sensing or cell-cell commu...
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PMID: 21031305
PDF is available here.
Abstract
Quorum sensing plays a central role in regulating many community-derived symbiotic and pathogenic relationships of bacteria, and as such has attracted much attention in recent years. Acyl-homoserine lactones (AHLs) are important signaling molecules in the quorum sensing gene-regulatory processes fou...
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PMID: 21031311
PDF is available here.
Abstract
We describe a set of western blot-based methodologies for the evaluation of biochemical effects specifically induced by N-(3-oxo-acyl) homoserine lactones (3-oxo-AHLs) small molecules secreted by a number of Gram-negative bacteria, including the opportunistic human pathogen Pseudomonas aeruginosa. F...
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PMID: 21031309
PDF is available here.
Abstract
The comet assay is a sensitive electrophoretic method for measuring DNA breaks at the level of single cells, used widely in genotoxicity experiments, biomonitoring, and in fundamental research. Its sensitivity and range of application are increased by the incorporation of an extra step, after lysis...
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PMID: 20972751
PDF is available here.
Abstract
The storage of the different microorganisms over long periods is necessary to ensure reproducible results and continuity in research and in biomedical processes and also for commercial purposes. Effective storage means that a microorganism is maintained in a viable state free of contamination or gen...
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PMID: 20949399
PDF is available here.
Abstract
We argue that the dynamics of within-host evolution has implications at the within-host and at the between-host levels. We first show that epidemiologists should consider within-host evolution, notably because it affects the genotype of the pathogen that is transmitted. We then present studies that...
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PMID: 21055948
PDF is available here.
Abstract
I propose classifying two phylogenetic clusters as separate species only when they have statistically significantly diverged as a result of adaptive evolution. More than a method for classification, the concept of adaptive divergence can be used in a 'reverse ecology' approach to identify lineages t...
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PMID: 21071229
PDF is available here.
Abstract
The ingestion of fatty meals is associated with a transient, low-grade systemic inflammatory response in human subjects, involving the activation of circulating monocytes and the secretion of pro-inflammatory cytokines. However, it is not yet clear how different foodstuffs may promote inflammatory s...
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PMID: 20849668
PDF is available here.
Abstract
We hypothesised that bovine colostrum and milk formulas enriched with sialic acids (SL), gangliosides (Gang) or osteopontin (OPN) would improve gastrointestinal function and NEC resistance in preterm neonates. Forty-seven caesarean-delivered preterm pigs were given total parenteral nutrition for 2 ...
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PMID: 20723273
PDF is available here.
Author(s) unavailable
Abstract
The principle of action, advantages and different types of Petrifilm as alternative test to the classical microbiological analysis are considered. Petrifilm is up-to-date high technology test systems for the rapid quantitative microbiological control in foodstuff of sanitary-significant and pathogeni...
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PMID: 21574466
PDF is available here.
Abstract
We think that the test of Theobald is not sufficient enough to reject the alternative hypothesis of the separate origins of life, despite the Akaike information criterion (AIC) of model selection giving a clear distinction between the competing hypotheses....
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PMID: 21164432
PDF is available here.
Abstract
We have investigated the molecular basis for the action mode of xylanases 10B at high temperatures using biochemical, biophysical and crystallographic methods. The crystal structure of xylanase 10B from hyperthermophilic bacterium Thermotoga petrophila RKU-1 (TpXyl10B) has been solved in the native...
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PMID: 21070746
PDF is available here.
Abstract
Our work highlighted that treated waters displayed residual toxicity after 24h still inducing mortality depending on the organism and biocide. However Mexel(®) 432/336 is the only biocide which had no impact on oyster larvae development at effective concentration. Altogether our data showed that Me...
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PMID: 21056457
PDF is available here.
Abstract
We present a simple, novel one-pot assay for the analysis of antibiotic susceptibility of bacterial biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) clearly confirm that the co...
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PMID: 20938507
PDF is available here.
Abstract
We report a low cost, disposable polymer microfluidic sample preparation device to perform rapid concentration of bacteria from liquid samples using enhanced evaporation targeted at downstream detection using surface enhanced Raman spectroscopy (SERS). The device is composed of a poly(dimethylsiloxa...
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PMID: 20938505
PDF is available here.
Abstract
The spectroscopic behavior of lumichrome (7,8-dimethyl-alloxazine, LC) in aqueous solutions in a pH range from -1.08 to 14.6 is studied. Absorption spectra, fluorescence quantum distributions, quantum yields, and lifetimes are determined. The ionization stage of ground-state LC changes with rising pH...
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PMID: 21091486
PDF is available here.
Abstract
RNA-based pathways that regulate protein expression are much more widespread than previously thought. Regulatory RNAs, including 5' and 3' untranslated regions next to the coding sequence, cis-acting antisense RNAs and trans-acting small non-coding RNAs, are effective regulatory mole...
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PMID: 21079634
PDF is available here.
Abstract
To explore the relationship between the bacterial biofilm, allergy, inflammatory cytokines and refractory rhinosinusitis.
According to the assessment of symptom by visual analogue scale and nasal endoscopy by Lund-Kennedy system, 19 patients with persistent chronic rh...
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PMID: 21215049
PDF is available here.
Abstract
We report a metagenomic analysis of a healthy human plaque sample using a combination of second-generation sequencing platforms. A total of 860 million base pairs of non-human sequences were generated. Various analysis tools revealed the presence of 12 well-characterized phyla, members of the TM-7 a...
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PMID: 21040513
PDF is available here.
Abstract
We examined earlier data indicating its role in pathogenesis. We conclude that although T6S can, in rare instances, directly influence interactions with higher organisms, the broader physiological significance of the system is likely to provide defense against simple eukaryotic cells and other bacte...
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PMID: 20961764
PDF is available here.
S J Olsen,
S Thamthitiwat,
S Chantra,
M Chittaganpitch,
A M Fry,
J M Simmerman,
H C Baggett,
T C T Peret,
D Erdman,
R Benson,
D Talkington,
L Thacker,
M L Tondella,
J Winchell,
B Fields,
W L Nicholson,
S Maloney,
L F Peruski,
K Ungchusak,
P Sawanpanyalert and
S F Dowell
Abstract
Although pneumonia is a leading cause of death from infectious disease worldwide, comprehensive information about its causes and incidence in low- and middle-income countries is lacking. Active surveillance of hospitalized patients with pneumonia is ongoing in Thailand. Consenting patients are teste...
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PMID: 20353622
PDF is available here.