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Blood Cells (4):
Recent article
Blood Cells
Xenobiotica (41)5 2011
Abstract
Protein binding of [14C]glucosamine (400, 1000 and 4000 ng/ml) was evaluated in human and mouse plasma and in human synovial fluid. Blood cell/plasma partitioning in human and mouse was also determined. There was no measurable protein binding of [14C]glucosamine. Its association with human and mouse...
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PMID: 20408501
PDF is available here.
PDF is available here.
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Abstract
We determined the inflammatory cell concentrations in blood and sputum, nitric oxide in exhaled air (FeNO), C-reactive protein (CRP) in plasma, cytokines (IL-6, 8, 1β, 10, 12, TNF-α) and SLPI (leukocyte protease inhibitor) and total antioxidant status (TAS) in blood and sputum, the activity of nuc...
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PMID: 21454005
PDF is available here.
PDF is available here.
Abstract
We show the derivation of iPSCs from human nonmobilized peripheral blood (PB) and bone marrow (BM) mononuclear cells (MNCs) by retroviral transduction of OCT3/4, SOX2, KLF4, and c-MYC. The PB- and BM-derived iPSCs were quite similar to human embryonic stem cells with regard to morphology, expression...
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PMID: 20497033
PDF is available here.
PDF is available here.
Abstract
We examined CCL23 production and release by human eosinophils in vitro.
Using Ficoll and antibody-coated immunomagnetic beads, eosinophils and other blood cells were purified from peripheral blood samples obtained from normal subjects and mildly allergic patients. Eosinophils were cu...
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PMID: 21646793
PDF is available here.
PDF is available here.
Abstract
Systemic available circulating cells play a role in cardiac maintenance and ameliorate cardiac recovery and repair after myocardial infarction. However, only a small number of cells will be incorporated during cardiac damage. Cell mobilization, homing to the ischemic myocardium and e...
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PMID: 20528755
PDF is available here.
PDF is available here.
Abstract
We are convinced that it will facilitate communication among experts and in the wider scientific community....
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PMID: 20628149
PDF is available here.
PDF is available here.
Clinical Immunology (137)1 2010
Abstract
We employed in vitro and in vivo models of sepsis to investigate the functional relationship between overtly produced C5a and IL-8. Our data revealed that C5a could strongly amplify IL-8 expression from human whole blood cells induced by LPS and other types of TLR agonists. ERK1/2 and p38, but not J...
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PMID: 20591742
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)10 2010
Abstract
to observe the effect of ICA on the immunosuppressive and bone-marrow-suppressive mice after chemotherapy, and explore the machanism of hematopoietic and immunologic function in mice accentuated by ICA.
mice were injected Cy intraperitoneally except control group , then randomly divided into mode gr...
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PMID: 20937233
PDF is available here.
PDF is available here.
Abstract
Increasing cholinergic activity has been the primary mechanism for treating dementia due to Alzheimer's disease. However, the effectiveness of cholinesterase inhibitors (ChEIs) is still widely debated. The identification of specific biomarkers capable of identifying patients more lik...
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PMID: 20185375
PDF is available here.
PDF is available here.
Journal of Biophotonics (3)8-9 2010
Abstract
We present the Raman spectroscopic characterization of different cells that can be found in peripheral blood such as leukocytes, leukemic cells and solid tumor cells. Leukocytes were isolated from the peripheral blood from healthy donors. Breast carcinoma derived tumor cells (MCF-7, BT-20) and myelo...
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PMID: 20449831
PDF is available here.
PDF is available here.
Molecular Membrane Biology (27)4-6 2010
Abstract
Vascular-targeted drug delivery systems could provide more efficient and effective pharmaceutical interventions for treating a variety of diseases including cardiovascular, pulmonary, inflammatory, and malignant disorders. However, several factors must be taken into account when designing these syst...
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PMID: 20615080
PDF is available here.
PDF is available here.
Abstract
Ultrastructural and morphological analyses of a novel hemostatic agent, Ankaferd Blood Stopper (ABS), in comparison to its in vitro and in vivo hemostatic effects were investigated. High-resolution scanning electron microscopy (SEM) images accompanied with morphological analysis after topical applic...
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PMID: 19833624
PDF is available here.
PDF is available here.
Abstract
X chromosome aneuploidy <10% in female patients is a routinely used reporting limit in diagnostic cytogenetics. X aneuploidy (<10%) is commonly detected in women investigated for infertility or recurrent miscarriages. It is unclear if this aneuploidy is causally relevant or related to the culture pr...
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PMID: 20716267
PDF is available here.
PDF is available here.
Nature Protocols (5)7 2010
Abstract
Umbilical cord blood is a key source of stem cells for transplantation and regenerative medicine. To maximize each cord blood sample, it is important to analyze its cellular populations. Many cord blood banks focus on counts of total nucleated cells and/or cells that carry the CD34 a...
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PMID: 20595961
PDF is available here.
PDF is available here.
Abstract
We applied SCNP to a panel of compounds with reported inhibitory effects on Jak/Stat signaling using a novel system where modulation of multiple signaling cascades are simultaneously measured in discrete cell subsets in whole (ie, unfractionated) blood. Jak2 vs. Jak3 selectivity as well as "off-targ...
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PMID: 20158439
PDF is available here.
PDF is available here.
Biomaterials (31)18 2010
Abstract
We describe the development of a functional polypeptide and a simple available technology for surface modification of nanoparticles. A NS polypeptide, which is 100 amino acids composed of repeated units of four asparagine and one serine residue (NS), as a molecule for nanoparticle surface modificati...
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PMID: 20363023
PDF is available here.
PDF is available here.
Abstract
This protocol describes a rapid procedure for isolating DNA from rat tail or ear punches. The simplest version of the protocol can be scaled for use in 96-well (deep-well) plates. The quality of the DNA is sufficient for any polymerase chain reaction (PCR)-based genotyping approach.
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PMID: 20439421
PDF is available here.
PDF is available here.
Abstract
Forty Holstein calves were used for the experiment and randomly assigned to four different treatments. Treatments consisted of control (injections of 5 ml normal saline), test 1 (500 mg Fe injected to each calf at 24-48 h of age), test 2 (200,000 IU vitamin A was injected to each calf at 24-48 h of...
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PMID: 20188781
PDF is available here.
PDF is available here.
Abstract
We show that the leukemia-associated Ets transcription factor, Tel1/ETV6, specifies the first hematopoietic stem cells (HSCs) in the dorsal aorta (DA). In contrast, Tel1/ETV6 has little effect on embryonic blood formation, further distinguishing the programming of the long- and short-term blood popu...
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PMID: 20412772
PDF is available here.
PDF is available here.
Abstract
We report that menstrual blood may be an important source of autologous stem cells. Immunocytochemical assays of cultured menstrual blood reveal that they express embryonic-like stem cell phenotypic markers (Oct4, SSEA, Nanog), and when grown in appropriate conditioned media, express neuronal phenot...
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PMID: 19860544
PDF is available here.
PDF is available here.
Abstract
BMC of donor irradiated by LDR in vitro may facilitate the hematopoietic reconstitution of the recipient mice....
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PMID: 20353342
PDF is available here.
PDF is available here.
Genome Research (20)4 2010
Abstract
We report the first genome-scale study of epigenomic dynamics during normal human aging. We identify aging-associated differentially methylated regions (aDMRs) in whole blood in a discovery cohort, and then replicate these aDMRs in sorted CD4(+) T-cells and CD14(+) monocytes in an independent cohort...
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PMID: 20219945
PDF is available here.
PDF is available here.
Abstract
Mathematical Biosciences (224)2 2010
Abstract
We propose a mathematical model of ordinary differential equations describing the dynamics of the HBV/HCV and its interaction with both liver and blood cells. A single model is used to describe infection of either virus since the dynamics in-host (infected of the liver) are similar. Analyzing the mo...
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PMID: 20085774
PDF is available here.
PDF is available here.
Abstract
The control group was characterized by the absence of blood elements on the surface. The best result was observed in the citric acid group, which had a dense fibrin network with blood elements adhered. The EDTA group showed a moderate fibrin network formation. In contrast, a scarce fibrin network an...
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PMID: 20305869
PDF is available here.
PDF is available here.
Applied Spectroscopy (64)4 2010
Abstract
Our results indicate that the erythrocyte PpIX profile could be used as a biological monitor for diabetes....
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PMID: 20412623
PDF is available here.
PDF is available here.
Abstract
We enriched tumor DNA by macrodissection and laser capture microdissection. LINE-1 methylation was calculated as an average of 100 * C/(C + T) at 4 CpG sites after bisulfite-PCR-pyrosequencing. The LINE-1 methylation value in colon cancers varied, ranging approximately from 30 to 80. To measure assa...
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PMID: 20093385
PDF is available here.
PDF is available here.
Georgian medical news ()180 2010
Abstract
Influence of various doses of xenobiotics on several biological and morphological indices of rat blood has been studied. It was established that intraperitoneal injections of the investigated phenols resulted in effect on biochemical indices of rat blood, specific to the particular phenolic compound...
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PMID: 20413822
PDF is available here.
PDF is available here.
Abstract
We address the influence of biomechanical factors on the arterial circulation. The first part presents our current understanding of the impact of blood flow on the arterial wall at the cellular level and the relationship between flow-induced stresses and the etiology of atherosclerosis. The second p...
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PMID: 20336826
PDF is available here.
PDF is available here.
Abstract
We show that age-associated changes in stem cell supportive niche cells deregulate normal haematopoiesis by causing haematopoietic stem cell dysfunction. Furthermore, we find that age-dependent defects in niche cells are systemically regulated and can be reversed by exposure to a young circulation o...
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PMID: 20110993
PDF is available here.
PDF is available here.
Journal of Asthma (47)1 2010
Abstract
We evaluated whether oxidative stress in the form of plasma reactive oxidants differs between asthmatic and non-asthmatic children and elucidate relationships between plasma reactive oxidants and other asthma-related immunological markers. Plasma reactive oxidants, white blood cell counts, total ser...
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PMID: 20100030
PDF is available here.
PDF is available here.
Abstract
We might expect differential miRNA regulation. Herein, we determined miRNA expression profiles of umbilical cord blood (UCB) cell lineages and compared them to those of bone marrow (BM) and peripheral blood (PB) cell counterparts. Further, we determined mRNA expression profiles using whole-genome mi...
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PMID: 19435428
PDF is available here.
PDF is available here.
Abstract
We used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms unde...
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PMID: 20010832
PDF is available here.
PDF is available here.
Abstract
We suggest that targeted gold nanopartices by tail vein injection may be suitable for enhancement of radiotherapy, photothermal therapy, and related medical diagnostic procedures....
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PMID: 21042423
PDF is available here.
PDF is available here.
Abstract
PGRMC1 levels did not vary significantly throughout the menstrual cycle. We observed a significant down-regulation of PGRMC1 in postmenopausal women and in patients with premature ovarian failure (POF) and polycystic ovary syndrome (PCOS) when compared to early follicular phase of healthy women. CON...
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PMID: 20537145
PDF is available here.
PDF is available here.
Abstract
Vorinostat (SAHA, Zolinza), a histone deacetylase inhibitor, is assessed in nonclinical studies to support its approval for cutaneous T-cell lymphoma. Vorinostat is weakly mutagenic in the Ames assay; is clastogenic in rodent (ie, CHO) cells but not in normal human lymphocytes; and is weakly positiv...
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PMID: 19903873
PDF is available here.
PDF is available here.
Abstract
We introduce an application of flow cytometry for the concurrent detection of phagocytotic cells and surface molecules involved in the phagocytic process. E. coli expressing green fluorescent protein (GFP) were applied as the phagocytosable particles. Blood samples were incubated with E. coli expres...
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PMID: 21038787
PDF is available here.
PDF is available here.
Abstract
The mean purity levels were at least 94% following isolation with Polymorphprep, the mean viability of neutrophils was 99%. CONCLUSION: The use of Polymorphprep technique provides a practical and effective method for the isolation of neutrophils from peripheral blood which can be used for investigat...
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PMID: 20499533
PDF is available here.
PDF is available here.
Abstract
Nasal mucosal and peripheral blood cells from beryllium production workers underwent cytochemical tests for catecholamines and phospholipids and the activity of esterase and lysosomal cationic proteins. The established peripheral blood metabolic changes reflect reduced cell resistance in response to...
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PMID: 20373717
PDF is available here.
PDF is available here.
Journal of Virology (84)1 2010
Abstract
We characterized PD-1 expression in cerebrospinal fluid (CSF) and blood of individuals with chronic human immunodeficiency virus type 1 (HIV-1) infection. PD-1 expression was higher on HIV-specific CD8(+) T cells than on total CD8(+) T cells in both CSF and blood. PD-1 expression on CSF T cells corr...
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PMID: 19828602
PDF is available here.
PDF is available here.
Tsitologiia (52)4 2010
Abstract
We have shown that the proper Feulgen fluorescence does not affect our measurements. Densitometric DNA content measurements of blood cells of four vertebrate species (Gallus domesticus, Danio rerio, Homo sapiens and Rana arvalis) showed good consistence to the literature data (http://www.genomesize....
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PMID: 20540347
PDF is available here.
PDF is available here.
Abstract
We describe a novel technology based on nanoengineered multifunctional acellular biologic scaffolds combined with wound dressings and films of the same kind. This method allows selective delivery and release of shielded biomaterials and bioactive substances to a desired wound or damaged tissue while...
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PMID: 20172347
PDF is available here.
PDF is available here.
Abstract
We also recognized swollen or crumpled red cells that looked like potato crisps. Aggregation of thickened blood platelets and white cells was observed frequently. Our study confirmed the importance of the smoothness of the inner surface of the catheter. Smoothness can prevent the formation of cell a...
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PMID: 19812925
PDF is available here.
PDF is available here.
Abstract
An 11 years old Yucatan Pig with a Multiple Myeloma in the spinal cord, the sternum and the pelvis is described. The major clinical sign was a paresis of the hind limb. The most significant haematological change was a lymphopenia. The X-ray examination and computed tomography showed typical osteolyt...
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PMID: 20135913
PDF is available here.
PDF is available here.
Abstract
We are presenting a series of four cases with raised MCHC, emphasizing the importance of systematic and meticulous examination of the peripheral smear to render a definitive diagnosis....
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PMID: 21045429
PDF is available here.
PDF is available here.
Parasitology (54)04 2009
Abstract
Abstract
We studied the effect of anxiolytic afobazole, exhibiting also antioxidant and antimutagenic properties, on spontaneous and ex-vivo hydrogen peroxide-induced DNA damage in blood cells from patients with systemic lupus erythematosus. Afobazole treatment (30-60 mg/day for 1 month) in addition to stand...
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PMID: 20396750
PDF is available here.
PDF is available here.
Abstract
We compared total BM-MNC numbers and its cellular subsets obtained following isolation using Ficoll-Paque and Lymphoprep - two different density gradient media used in the clinical trials. MATERIALS & METHODS: Bone marrow samples were taken from patients entered into the REGENERATE-IHD clinical tria...
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PMID: 19761394
PDF is available here.
PDF is available here.
Abstract
The authors consider recommendations on blood smear microscopic tests, possibilities of implementing these recommendations when manual microscopy and automatic microscopy complexes (AMC) are used. Whether one may switch over from the recommendations to the quality standard implemented in practice wh...
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PMID: 19882868
PDF is available here.
PDF is available here.
Journal of Asthma (46)7 2009
Abstract
We evaluated whether oxidative stress in the form of plasma reactive oxidants differs between asthmatic and non-asthmatic children and elucidate relationships between plasma reactive oxidants and other asthma-related immunological markers. Plasma reactive oxidants, white blood cell counts, total ser...
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PMID: 19728206
PDF is available here.
PDF is available here.
Xenobiotica (39)6 2009
Abstract
- Pharmacokinetics of liquiritigenin, a candidate for inflammatory liver disease, and its two glucuronide conjugates, M1 and M2, were evaluated in rats. The hepatic and gastrointestinal first-pass effects of liquiritigenin were also evaluated in rats.
- After oral administration of li...
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PMID: 19480552
PDF is available here.
Abstract
We cannot reach definite conclusions even if our data confirm the accumulation of DNA lesions in PD patients; these lesions decrease after LD intake....
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PMID: 18523852
PDF is available here.
PDF is available here.
Abstract
The treatment of certain diseases will require the systemic delivery of therapeutic genes or viruses. In most cases, intravascular injection is the best delivery method to achieve the systemic distribution of viruses and to enable these agents to reach distant therapeutic sites. However, viruses adm...
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PMID: 19649986
PDF is available here.
PDF is available here.
Pflege Zeitschrift (62)8 2009
Abstract
Pflege Zeitschrift (62)8 2009
Abstract
Abstract
We review the substantial in vitro, clinical trial and clinical practice observational evidence that non-irradiated INTERCEPT- and Mirasol-treated cellular blood components do not cause TA-GVHD. Implementation of these processes precludes the necessity for irradiating cellular blood components to pr...
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PMID: 19617907
PDF is available here.
PDF is available here.
Abstract
We have focused on a regulatory circuit playing a central role in myelopoiesis: the transcription factor PU.1, its target gene colony-stimulating-factor 1 receptor (Csf1r), and key upstream regulators such as RUNX1. We find that during ontogeny, chromatin unfolding precedes the establishment of acti...
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PMID: 19339695
PDF is available here.
PDF is available here.
Mitochondrion (9)4 2009
Abstract
Mitochondrial DNA (mtDNA) content is important for understanding many cellular processes. Several pre-analytical factors, from sample collection to DNA extraction can affect measurement of mtDNA copy number. In the present study, whole blood samples yielded a higher mtDNA copy number...
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PMID: 19272467
PDF is available here.
PDF is available here.
Mitochondrion (9)4 2009
Abstract
We report the clinical and genetic characterization of a Chinese Leber's hereditary optic neuropathy (LHON) family with complete penetrance and high percentage of recovery. Sequence analysis of the complete mitochondrial DNA revealed the presence of heteroplasmic ND6/T14484C mutation and 27 other va...
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PMID: 19394449
PDF is available here.
PDF is available here.