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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Protoplasts (0):
Recent article
Protoplasts
Plant Physiology (154)2 2010
Abstract
We describe here an in vivo method to rapidly determine gene function using transient expression of artificial microRNAs (amiRNAs) in Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts. We use a luciferase-based reporter of circadian clock activity to optimize and validate this system. Protopl...
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PMID: 20709829
PDF is available here.
PDF is available here.
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Abstract
Two different endophytic strains, ESRAA1997 and ALAA2000, were isolated from the Egyptian herbal plant Anastatica hierochuntica. The 2 strains produced alkaline serine protease and were identified based on their phenotypic and chemotypic characteristics as different strains of Micromonospora spp. Bo...
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PMID: 20921985
PDF is available here.
PDF is available here.
Marine Biotechnology (12)4 2010
Abstract
We found that the mechanisms of the inhibition may be that the beta-1,3-glucanase competed for binding to beta-1,3-glucan on the sensitive yeast cell wall with the WC91-2 toxin, causing decrease in the amount of the WC91-2 toxin bound to beta-1,3-glucan on the sensitive yeast cell wall and the activ...
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PMID: 19941023
PDF is available here.
PDF is available here.
Abstract
The wide application of rare earth elements (REEs) has led to the accumulation of REEs in soil and plant. Thereby, the effect of Tb(3+) on the contents of cytosolic nutrient elements in horseradish was investigated with the synchronous detection technique of scanning electron microscope and energy d...
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PMID: 20417560
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal catalytic domain for plasma membrane localization...
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PMID: 20427464
PDF is available here.
PDF is available here.
Abstract
Our data suggest that Ccrboh might be functionally important during the acclimation of plants to stress and also in plant development. It holds great promise for improving drought tolerance of other cucurbit species....
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PMID: 20181664
PDF is available here.
PDF is available here.
Carbohydrate Research (345)7 2010
Abstract
A water-soluble immunoenhancing polysaccharide was isolated from the aqueous extract of fruit bodies of somatic hybrid (Pflo Vv5 FB), obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains. On the basis of acid hydrolysis, the polysaccharide was found to contai...
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PMID: 20347070
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (26)3 2010
Abstract
Four dsRNA bands were extracted from Pleurotus ostreatus TD300 by the dsRNA isolation technique with sizes of 8.2 kb, 2.5 kb, 2.1 kb, and 1.1 kb, respectively. Four virus-eliminated methods, i. e. hyphal tips cut (HTC), protoplast regeneration (PR), single spore hybridization (SSH), and frozen and l...
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PMID: 20572349
PDF is available here.
PDF is available here.
Abstract
We identified transcription factors from a tomato cDNA microarray. An ABA-responsive element binding protein (AREB) was identified and named SlAREB. In tomato protoplasts, SlAREB transiently transactivated luciferase reporter gene expression driven by AtRD29A (responsive to dehydration) and SlLAP (l...
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PMID: 20358223
PDF is available here.
PDF is available here.
Abstract
The mariner-like transposon Mos1 is used for insertional mutagenesis and transgenesis in different animals (insects, nematodes), but has never been used in plants. In this paper, the transposition activity of Mos1 was tested in Nicotiana tabacum, but no transposition event was detected. In an attemp...
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PMID: 19847655
PDF is available here.
PDF is available here.
Abstract
We report the development of protoplast and transformation system for U. oxytropis. Fungal mycelia required for generating protoplasts were grown in liquid culture, then harvested and processed with various enzymes. Protoplasts were transformed with a fungal specific vector driving the expression of...
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PMID: 20211666
PDF is available here.
PDF is available here.
Journal of Cell Science (123)Pt 9 2010
Abstract
Reactive oxygen species (ROS) are central to plant stress response, signalling, development and a multitude of other processes. In this study, the plasma-membrane hydroxyl radical (HR)-activated K(+) channel responsible for K(+) efflux from root cells during stress accompanied by ROS generation is c...
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PMID: 20375061
PDF is available here.
PDF is available here.
Journal of Plant Physiology (167)6 2010
Abstract
The 2-Cys peroxiredoxin-A (2CPA) promoter is a model promoter to study redox and ABA-dependent stress signaling. Here, an Arabidopsis reporter gene line expressing luciferase under control of the 2CPA promoter was used to study the impact of ascorbate on reporter gene transcription in a series of pr...
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PMID: 20022402
PDF is available here.
PDF is available here.
Abstract
Morphologically, nine different slow-growing protoclones were screened from regenerated protoplast of heterokaryotic Agaricus bisporus. The present study is the first report of fingerprinting on differentiating homo- and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and in...
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PMID: 20467239
PDF is available here.
PDF is available here.
Plant Physiology (152)4 2010
Abstract
Manganese (Mn) is an essential element, acting as cofactor in numerous enzymes. In particular, a Mn cluster is indispensable for the function of the oxygen-evolving complex of photosystem II. Metal transporters of the Natural Resistance-Associated Macrophage Protein (NRAMP) family have the ability t...
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PMID: 20181755
PDF is available here.
PDF is available here.
Abstract
We studied the tissue-specific expression patterns of Rac/Rop genes by semi-quantitative reverse transcription-PCR (RT-PCR), and also Rac/Rop subcellular localization using green fluorescent protein (GFP) fusion proteins in transient expression systems. We also investigated the roles of these genes...
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PMID: 20203239
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)2 2010
Abstract
Protoplast preparation, regeneration and fusion represent essential tools for those poorly studied biotechnologically valuable microorganisms inapplicable with the current molecular biology protocols. The protoplast production and regeneration method developed for Planobispora rosea and using the co...
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PMID: 20057514
PDF is available here.
PDF is available here.
Abstract
High-resolution, cell type-specific analysis of gene expression greatly enhances understanding of developmental regulation and responses to environmental stimuli in any multicellular organism. In situ hybridization and reporter gene visualization can to a limited extent be used to this end but for h...
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PMID: 20168296
PDF is available here.
PDF is available here.
Abstract
Cell respiratory activity of protoplasts obtained from the wild type of Neurospora crassa and photoreceptor complex WCC--white collar 1 (wc-1) and white collar 2 (wc-2)--mutants of Neurospora crassa strains was investigated. Respiration inhibition by KCN in the presence of 25 mM succinate was simila...
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PMID: 20586289
PDF is available here.
PDF is available here.
FEBS Letters (583)22 2009
Abstract
We studied a stromal lamellae protein, Hcf106, by targeting an Hcf106-GFP fusion protein to the thylakoids and photobleaching. We find that even small regions fail to recover Hcf106-GFP fluorescence over periods of up to 3 min after photobleaching. The protein is thus either immobile within the thyl...
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PMID: 19854178
PDF is available here.
PDF is available here.
Abstract
Spinosad is a new class of insecticides produced by Saccharopolyspora spinosa. The aim of this study was to construct a starch-utilizing strain that overproduced spinosad by intergeneric fusion between S. spinosa and Streptomyces avermitilis. Protoplast fusion is an important technique for engineeri...
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PMID: 19898549
PDF is available here.
PDF is available here.
Abstract
The cyclic undecapeptide cyclosporin A (CyA), one of the most valuable immunosuppressive drugs, is produced nonribosomally by a multifunctional cyclosporin synthetase enzyme complex by the filamentous fungus Tolypocladium niveum. To increase CyA productivity by wild-type T. niveum (ATCC 34921), rand...
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PMID: 19809241
PDF is available here.
PDF is available here.
Abstract
We apply nuclear proteome analysis to the search for novel nuclear proteins that play evolutionarily conserved roles in the sugar response in plants. We initially identified 563 different proteins by nanoLC/ESI/MS/MS analysis of extracts from rice nuclei that were purified by Percoll density gradien...
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PMID: 19621931
PDF is available here.
PDF is available here.
Plant Physiology (150)4 2009
Abstract
Caspase-like proteases have been demonstrated to be involved in plant programmed cell death (PCD). Here, the time scale of caspase-3-like protease activation was investigated in single living plant cells undergoing PCD induced by ultraviolet C (UV-C) overexposure. The real-time detection of caspase-...
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PMID: 19535476
PDF is available here.
PDF is available here.
Plant Molecular Biology (70)6 2009
Abstract
We have engineered Cre-lox UPS host acceptor vectors (pCR701- 705) with N-terminal epitope tags in frame with the loxH site and downstream from the maize Ubiquitin promoter for use in transient protoplast expression assays and particle bombardment transformation of monocots. As an example of the uti...
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PMID: 19499346
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
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PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
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PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
We found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the...
|
PMID: 19446304
PDF is available here.
PDF is available here.
Abstract
The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in...
|
PMID: 19443005
PDF is available here.
PDF is available here.