Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Protein Denaturation (0):
Recent article
Protein Denaturation
Abstract
Our results indicate that the stability of the complexes between W and proteins is not too high enough to remain unaltered during protein separation by SDS-PAGE in denaturing and reducing conditions. However, the procedures for in-solution tryptic digestion and for ESI-MS analysis in MeOH/H(2)O/with...
|
PMID: 21530773
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Biochimica et Biophysica Acta (1814)9 2011
Abstract
We studied the effects of a chemically modified β-CD (BCD07056), on the aggregating and refolding properties of BlaPChBD, a hybrid protein obtained by inserting the chitin binding domain of the human macrophage chitotriosidase into the class A β-lactamase BlaP from Bacillus licheniformis 749/I dur...
|
PMID: 21621654
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We developed a two-step strategy for the enrichment of low-abundant soluble chloroplast proteins from Pisum sativum and their subsequent identification by MS. First, chloroplast protein extracts were depleted from the most abundant protein ribulose-1,5-bisphosphate carboxylase/oxygenase by SEC or he...
|
PMID: 21365755
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)9 2011
Abstract
We used the infrared amide I' band to study the protein conformation, and the position of the antisymmetric stretching band of the methylene groups in the lipid hydrocarbon chains to study the lipid order. Lipid-protein complexes were studied in media of low and high ionic strengths. In solution, β...
|
PMID: 21600190
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)9 2011
Abstract
We study the folding pathway for purified LamB protein in detergent and observe extreme hysteresis in unfolding and refolding, as indicated by the shift in intrinsic fluorescence. The strong hysteresis is not seen in unfolding and refolding a mutant LamB protein lacking the disulfide bond, as it unf...
|
PMID: 21640073
PDF is available here.
PDF is available here.
Abstract
We have examined systematically the effects of protein size and sequence on the value of ΔV(u) using as a model system a series of deletion variants of the ankyrin repeat domain of the Notch receptor. The results provide strong evidence in support of the notion that the major contributing factor to...
|
PMID: 21446709
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)7 2011
Abstract
We have used solid and solution-phase assays to analyze the in vitro binding of calreticulin, purified from human placenta, to human IgG and chicken IgY in order to compare the interactions. In addition, peptides from the respective immunoglobulins were included to further probe the binding specific...
|
PMID: 21447409
PDF is available here.
PDF is available here.
Abstract
Several neurodegenerative diseases share a common neuropathology, primarily featuring the presence of abnormal protein inclusions in the brain containing specific misfolded proteins. Strategies to decrease the load of protein aggregates and oligomers are considered relevant targets f...
|
PMID: 21252621
PDF is available here.
PDF is available here.
Abstract
The increasing appreciation of the importance of autophagy as consequence of cancer therapy or underlying disease biology is illustrated by the large number of papers that are evaluating autophagy as a cancer target. While autophagy is often linked to the generation of metabolic prec...
|
PMID: 21242725
PDF is available here.
PDF is available here.
Abstract
Abstract
To search for agents affecting thermal stability of proteins, a test based on the registration of protein aggregation in the regime of heating with a constant rate was used. The initial parts of the dependences of the light scattering intensity (I) on temperature (T) were analyzed us...
|
PMID: 21760963
PDF is available here.
PDF is available here.
Abstract
The surface-enhanced laser desorption ionization (SELDI) technology is a promising approach not only for the research of biomarkers in the blood of patients in clinical applications but also in preclinical studies to assess the drug-induced toxicities. The optimization of the SELDI platform is a cru...
|
PMID: 20972765
PDF is available here.
PDF is available here.
Abstract
We describe efficient procedures for the isolation of highly purified His-tagged target proteins from an Escherichia coli host using IMAC....
|
PMID: 20978973
PDF is available here.
PDF is available here.
Abstract
Expression of recombinant proteins in the baculovirus/insect cell expression system is employed because it enables post-translational protein modification and high yields of recombinant protein. The system is capable of facilitating the functional expression of many proteins - either secreted or int...
|
PMID: 20978972
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)4 2011
Abstract
We have recently shown [Vecino et al., Biochim. Biophys. Acta 1798(11), 2160-2169 (2010)] that TrwB can be reconstituted into liposomes, and that bilayer incorporation increases its affinity for nucleotides and its specificity for ATP. In the present contribution we examine the structural effects of...
|
PMID: 21211515
PDF is available here.
PDF is available here.
Abstract
We examined the conformational transitions in a multimeric and high molecular weight class II α-mannosidase from Canavalia ensiformis (Jack Bean) (Jbα-man) utilizing intrinsic fluorescence, solute quenching, hydrophobic dye binding, size exclusion chromatography and circular dichroism (CD) spectro...
|
PMID: 21087596
PDF is available here.
PDF is available here.
Abstract
Abstract
We here studied the globular domain of this mutant protein by molecular dynamics simulations. Compared to the wide-type protein, the mutant has similar dynamics and stability profiles in our simulation. Conformational rearrangements are concentrated around the mutation site, due to the introduction...
|
PMID: 20919751
PDF is available here.
PDF is available here.
Abstract
We used scaled particle theory (SPT) to explain these experimental observations. The effective length, called Tolman's length that describes the curvature correlations to a surface area or surface tension and in turn contributes to the change in free energy, is discussed. Tolman's length is higher f...
|
PMID: 20919749
PDF is available here.
PDF is available here.
EMBO Journal (29)23 2010
Abstract
We found that, in the rate-limiting intermediate of a chaperonin reaction, the unfolded polypeptide in the cage partly protrudes through a narrow space near the GroEL/GroES interface. Then, the entire polypeptide is released either into the cage or to the outside medium. The former adopts a native s...
|
PMID: 20959808
PDF is available here.
PDF is available here.
Abstract
A polypeptide chain can adopt very different conformations, a fundamental distinguishing feature of which is the water accessible surface area, WASA, that is a measure of the layer around the polypeptide chain where the center of water molecules cannot physically enter, generating a solvent-excluded...
|
PMID: 20882232
PDF is available here.
PDF is available here.
British Journal of Nutrition (104)10 2010
Abstract
I to amide II spectral intensity and their ratio) and protein rumen degradation kinetics (rate and extent), estimated protein intestinal digestibility and total truly absorbed protein in small intestine (metabolisable protein) in different types of dried distillers grains with solubles (DDGS), such...
|
PMID: 20594396
PDF is available here.
PDF is available here.
Abstract
We extend such work to flexible, multidomain proteins and one of the most widespread membrane transporter families, the major facilitator superfamily, thus showing that more complex membrane proteins can be successfully refolded to recover native substrate binding. We determine the unfolding free en...
|
PMID: 20937906
PDF is available here.
PDF is available here.
Biophysical Chemistry (151)3 2010
Abstract
Dielectric relaxation (DR) study was performed to reveal the hydration change of Pseudomonas aeruginosa ferric cytochrome c551 (PA c551) in dilute aqueous solutions upon the acid unfolding which undergoes a two-state transition. The DR spectrum of a small spherical region containing a PA c551 molecu...
|
PMID: 20630646
PDF is available here.
PDF is available here.
Biophysical Chemistry (151)3 2010
Abstract
The "hydrophobic hydration processes" can be satisfactorily interpreted on the basis of a common molecular model for water, consisting of two types of clusters, namely W(I) and W(II) accompanied by free molecules W(III). The principle of thermal equivalent dilution (TED) is the potent tool (Ergodic...
|
PMID: 20656401
PDF is available here.
PDF is available here.
Abstract
To investigate the sequence dependence in the molecular mechanism of urea induced denaturation, molecular dynamics denaturing simulations of two beta-hairpin peptides, a fast folding peptide 1 (SESYINPDGTWTVTE) and a slow folding TRPZIP4 (GEWTWDDATKTWTWTE), were performed in urea aqueous solutions....
|
PMID: 20571679
PDF is available here.
PDF is available here.
Abstract
We therefore conclude that urea interacts favorably with the whole protein surface and that all parts of the protein are important in urea-induced denaturation....
|
PMID: 20563326
PDF is available here.
PDF is available here.
Abstract
The triggering of Ca(2+) signaling pathways relies on Ca(2+)/Mg(2+) specificity of proteins mediating these pathways. Two homologous milk Ca(2+)-binding proteins, bovine alpha-lactalbumin (bLA) and equine lysozyme (EQL), were analyzed using the simplest "four-state" scheme of metal- and temperature-...
|
PMID: 20602456
PDF is available here.
PDF is available here.
Abstract
Protein structure and function can be regulated by no specific interactions, such as ionic interactions in the presence of salts. Green fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; its stability can be directly related to its fluorescence out...
|
PMID: 20865748
PDF is available here.
PDF is available here.
Abstract
We measured the effects of various mutations introduced to the L2 loop's region (residues 228-238) on the stability of HtrA molecule and its proteolytic activity. We demonstrated that most mutations affected the activity of HtrA. In the case of the following substitutions: L229N, N235I, I238N, the p...
|
PMID: 20515644
PDF is available here.
PDF is available here.
Abstract
We interrogated the folding landscape of a model beta-rich protein, cellular retinoic acid-binding protein I (CRABP I), in the presence of an inert crowding agent (Ficoll 70). Urea titrations revealed a crowding-induced change in the water-accessible polar amide surface of its denatured state, based...
|
PMID: 20662522
PDF is available here.
PDF is available here.
Abstract
To improve toxoid preparation, the effects of selective heat denaturation were assessed on Deinagkistrodon acutus venom. The venom and its fractions (peak 1 and peak 2 separated by gel filtration chromatography) were heated to various temperatures (45-70 degrees C) for 30 min, after which protein co...
|
PMID: 20331994
PDF is available here.
PDF is available here.
Abstract
Catalysis by rabbit muscle pyruvate kinase involves domain movements and conformational changes induced by activating cations and its substrates. Fluorescence acrylamide quenching analyses reveal that interactions with Mg(2+) and K(+) lead to a more exposed active site of PK while interactions with...
|
PMID: 20435056
PDF is available here.
PDF is available here.
Abstract
A novel keratinase from Chryseobacterium sp. strain kr6 was purified to homogeneity by (NH(4))(2)SO(4) precipitation, gel permeation on Sephadex G-100, and Q-Sepharose Fast Flow anion-exchange chromatography. The molecular weight of the purified enzyme was around 20 kDa. Kinetic and thermodynamic pa...
|
PMID: 19936635
PDF is available here.
PDF is available here.
Abstract
We investigated the effect of different polyol compounds, e.g. glycerol, mannitol and PEG-6000 on the in vitro stability of purified hPAH produced in a heterologous prokaryotic expression system. The recombinant human enzyme was stored in the presence of the studied stabilizing agents at different t...
|
PMID: 19937396
PDF is available here.
PDF is available here.
Journal of Molecular Biology (401)5 2010
Abstract
We note that many molecular chaperones possess a substantial negative charge that may allow them to impart solubility on aggregation-prone proteins. Hsp90 is one such negatively charged molecular chaperone. The charge on Hsp90 is largely concentrated in two highly acidic regions. To investigate the...
|
PMID: 20615417
PDF is available here.
PDF is available here.
Abstract
Journal of Fluorescence (20)4 2010
Abstract
The conformational transitions in an oligomeric and high molecular weight class II alpha-mannosidase from Aspergillus fischeri were examined using fluorescence and CD spectroscopy under chemical, thermal and acid denaturing conditions. The enzyme lost the activity first and then the overall folded c...
|
PMID: 20204683
PDF is available here.
PDF is available here.
Abstract
We show here that Saccharomyces cerevisiae Ubr1 and Ubr2 ubiquitin ligases promote degradation of unfolded or misfolded cytosolic polypeptides. Ubr1 also catalyzes ubiquitinylation of denatured but not native luciferase in a purified system. This activity is based on the direct interaction of denatu...
|
PMID: 20462952
PDF is available here.
PDF is available here.
Meat Science (85)3 2010
Abstract
The effects of freeze-thaw cycles (FT, 0, 1, 3 and 5 times) on protein functional properties of porcine longissimus muscle were investigated. FT increased gapping between muscle fibres and tore muscle fiber bundles. Myofibrillar protein (MP) isolated from FT muscle showed an increased hydrophobicity...
|
PMID: 20416818
PDF is available here.
PDF is available here.
Abstract
We have prepared a set of yeast iso-1-cytochrome c variants that contain polyalanine inserts ranging from 6 to 30 residues in length near the N terminus of the protein. We study the thermodynamics and kinetics of His-heme loop formation in the denatured state at 3 and 6 M guanidine-HCl concentration...
|
PMID: 20534569
PDF is available here.
PDF is available here.
Abstract
This study characterizes three monoclonal antibodies (mAbs) developed against the constant (C) region of the immunoglobulin light (IgL) sigma chain isotype of the channel catfish, Ictalurus punctatus. Microsphere bead assays and Western blot analyses utilizing different recombinant (r) proteins show...
|
PMID: 20149930
PDF is available here.
PDF is available here.
Abstract
A new method for identifying residue specific through space contacts as a function of protein secondary and tertiary structure in the gas phase is presented. Photodissociation of a non-native carbon-iodine bond incorporated into Tyr59 of ubiquitin yields a radical site specifically at that residue. T...
|
PMID: 20524634
PDF is available here.
PDF is available here.
Abstract
We use a single-molecule optical tweezers approach to induce the selective unfolding of particular regions of T4 lysozyme and monitor the effect on other regions not directly acted on by force. We investigate how the topological organization of a protein (the order of structural elements along the s...
|
PMID: 20495548
PDF is available here.
PDF is available here.
Journal of Molecular Biology (399)2 2010
Abstract
Conformational change and aggregation of native proteins are associated with many serious age-related and neurological diseases. gammaS-Crystallin is a highly stable, abundant structural component of vertebrate eye lens. A single F9S mutation in the N-terminal domain of mouse gammaS-crystallin cause...
|
PMID: 20382156
PDF is available here.
PDF is available here.
Laboratory Investigation (90)6 2010
Abstract
I mRNA. The aim of this study was to elucidate polyamines role in EMT in vitro. Madin-Darby canine kidney (MDCK) cells were subjected to experimental manipulation of intracellular levels of polyamines. Acquisition of mesenchymal phenotype was evaluated by means of immunofluorescence, western blots,...
|
PMID: 20212449
PDF is available here.
PDF is available here.
Abstract
We previously reported that deamidation at the critical interface sites decreased stability, while preserving the dimeric 3D structure. In the present study, dynamic light scattering, differential scanning calorimetry and small angle X-ray scattering were used to investigate the effect of deamidatio...
|
PMID: 20188088
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1803)6 2010
Abstract
Recognition and elimination of misfolded proteins are essential cellular processes. More than thirty percent of the cellular proteins are proteins of the secretory pathway. They fold in the lumen or membrane of the endoplasmic reticulum from where they are sorted to their site of action. The folding...
|
PMID: 20219571
PDF is available here.
PDF is available here.
Abstract
We engineered the K18N, L54R, D165V, A168D, L172H, and Y231N mutations into the full-length dystrophin cDNA and characterized the biochemical properties of each mutant protein. The K18N and L54R mutations are associated with the most severe diseases in humans and each caused a small but significant...
|
PMID: 20457930
PDF is available here.
PDF is available here.
Journal of Chromatography A (1217)19 2010
Abstract
Interferon alpha-2 (IFN alpha-2) products have been widely used as antivirals for the treatment of serious diseases such as hepatitis B and C. However, reports of adverse reactions following treatment have prompted investigations into the cause of these undesirable events. In this study size-exclusi...
|
PMID: 19931086
PDF is available here.
PDF is available here.
Abstract
Diagnosis of HBoV primary infection can be strengthened by measurement of IgG avidity. HBoV secondary infections or anamnestic antibody responses occur ubiquitously in immunocompetent adults.
Copyright 2010 Elsevier B.V. All rights reserved....
|
PMID: 20227338
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (114)16 2010
Abstract
Atomistic dynamics simulations of purely hydrophobic carbon nanotubes in 8 M urea are performed to dissect the role of dispersion interactions in the denaturing power of urea. The enhanced population of urea and a paucity of water in proximity of nanotubes suggest that the stronger dispersion intera...
|
PMID: 20361766
PDF is available here.
PDF is available here.
Abstract
We have developed a rapid and accurate denaturing high pressure liquid chromatography (dHPLC) method of the promoter, 3'UTR, all exons including exon/intron boundaries as a first line screen followed by direct sequencing of the respective PCR products. To validate this method we used DNA from 23 dif...
|
PMID: 20083419
PDF is available here.
PDF is available here.
Abstract
We have used a synthetic peptide approach to investigate three stability control sites within the stability control region (residues 97-118). Two of the sites, electrostatic cluster 1 (97-104, EELDRAQE) and electrostatic cluster 2 (112-118, KLEEAEK), feature sequences with unusually high charge dens...
|
PMID: 20144718
PDF is available here.
PDF is available here.
Abstract
We identified an arylstibonic acid, NSC13778 that specifically binds to the basic region of the C/EBPalpha B-ZIP domain and disrupts DNA binding. We now examine a panel of 14 additional arylstibonic acid derivatives of NSC13778 for their ability to inhibit the DNA binding of five B-ZIP dimers (c-Fos...
|
PMID: 20176111
PDF is available here.
PDF is available here.
Abstract
We previously showed that the protein carrying the mutation responsible for late-onset cblC (MMACHC-R161Q), treatable with high dose OHCbl, is able to bind OHCbl with wild-type affinity, leaving undetermined the disease mechanism involved [Froese et al., Mechanism of responsiveness, Mol. Genet. Meta...
|
PMID: 20219402
PDF is available here.
PDF is available here.
Abstract
We demonstrated that a pair of positions in phosphoglycerate kinase that score highly by three nonparametric covariation measures are important for function even though the positions can be occupied by aliphatic, aromatic, or charged residues. Examination of these pairs suggested that the majority o...
|
PMID: 20065119
PDF is available here.
PDF is available here.
Abstract
We not only developed a few new compounds showing the AIE phenomena but also controlled the AIE through environmental stimulation and demonstrated that the new AIE molecules are suitable for pH and biomacromolecule sensing....
|
PMID: 20112939
PDF is available here.
PDF is available here.
Biochemistry (New York) (75)5 2010
Abstract
We demonstrated that tetrameric BkLAP exists as the major form in solution at protein concentration of 1.5 mg/ml at pH 8.0. The native enzyme started to unfold beyond ~1 M GdnHCl and reached an unfolded intermediate with [GdnHCl](1/2) at 1.8 M. Thermal unfolding of BkLAP was found to be highly irrev...
|
PMID: 20632945
PDF is available here.
PDF is available here.
Abstract
We study the folding-unfolding kinetics of a beta-peptide that folds into a 14-helical structure in water. We find that the T-jump induced relaxation kinetics of this peptide occur on the nanosecond time scale and are noticeably slower than those of alanine-based alpha-helical peptides, and addition...
|
PMID: 20373737
PDF is available here.
PDF is available here.