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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Protein Denaturation (0):
Recent article
Protein Denaturation
Biopolymers (96)1 2011
Abstract
We present a comprehensive study on the thermodynamic and kinetic consequences of replacing Gly residues at different sites in collagen. For this, we prepared a series of peptides that contain a single substitution of Gly with L-Ala, D-Ala, β-Ala, or sarcosine (Sar), at different positions in a hos...
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PMID: 20560144
PDF is available here.
PDF is available here.
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Abstract
Hydrophilic ionic liquid-polyvinyl chloride (PVC) hybrids were prepared by immobilizing N-methylimidazole (N-mim) to PVC chains in toluene. The NmimCl-PVC hybrids were characterized by FT-IR, (1)H NMR, surface charge analysis and elemental analysis. The immobilization ratio, i.e., the percentage of...
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PMID: 20188975
PDF is available here.
PDF is available here.
Abstract
This paper is aimed at investigating the conformational change of denatured bovine serum albumin (BSA) in combination with thermodynamic functions and their fractions, adsorption isotherms, Fourier transform infrared (FTIR) spectroscopy, and differential scanning calorimetry (DSC). Microcalorimetric...
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PMID: 20138631
PDF is available here.
PDF is available here.
Biophysical Journal (98)8 2010
Abstract
I and the folding nucleus were derived solely from kinetic data, namely, the slow-phase folding rate constants and the burst-phase amplitudes of Trp fluorescence intensity. This allowed us to pioneer the phi-analysis for apomyoglobin. As shown, these mutations drastically destabilized the native sta...
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PMID: 20409491
PDF is available here.
PDF is available here.
Analytical Biochemistry (399)2 2010
Abstract
Our data suggest that melting curves can help to distinguish properly folded proteins from denatured ones and, therefore, may be useful in selecting stocks for further study and in optimizing purification procedures for specific proteins.
Copyright 2009 Elsevier Inc. All rights reserved....
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PMID: 20018159
PDF is available here.
PDF is available here.
Biophysical Journal (98)8 2010
Abstract
We have effectively reproduced such similarities and differences in behavior as are interpreted from experiment. From the analysis of such data, we explain the experimental evidence at hand through the effect of pressure on the change of water structure, and hence the weakening of the hydrophobic ef...
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PMID: 20409483
PDF is available here.
PDF is available here.
Biophysical Journal (98)8 2010
LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment.
Abstract
We have developed a robust and accurate assay, LCP-Tm, for measuring the thermal stability of membrane proteins embedded in an LCP matrix. In its two implementations, protein denaturation is followed either by a change in the intrinsic protein fluorescence on ligand release, or by an increase in the...
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PMID: 20409473
PDF is available here.
PDF is available here.
Abstract
We address this problem by using a combination of accelerated calculations on graphics processor units and distributed computing to simulate tens of thousands of molecular dynamics trajectories each up to approximately 10 mus (for a total aggregate simulation time of 127 ms). We used this approach i...
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PMID: 20218718
PDF is available here.
PDF is available here.
Abstract
Cold denaturation is a general property of globular proteins, and the process provides insight into the origins of the cooperativity of protein folding and the nature of partially folded states. Unfortunately, studies of protein cold denaturation have been hindered by the fact that the cold denature...
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PMID: 20225821
PDF is available here.
PDF is available here.
Abstract
Protein refolding using urea gradient size exclusion chromatography (UGSEC) is a new version of conventional SEC refolding, which incorporates urea gradient into the SEC. Operating factors, mainly the urea gradient length and the final urea concentration in the gradient, are discussed in detail. Rec...
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PMID: 20210741
PDF is available here.
PDF is available here.
Abstract
We have developed a high-pH, pH-shift refolding "Ph-Fold technology", both for academic research and industrial protein drug development applications. Using this technology, we were able to refold some "difficult-to-refold" proteins, some of which are proven important drug targets and protein drug c...
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PMID: 20210740
PDF is available here.
PDF is available here.
Abstract
We review the cyclodextrin (CD)-related protein refolding systems....
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PMID: 20210739
PDF is available here.
PDF is available here.
Abstract
We often observe distribution of expressed proteins in both soluble and insoluble fractions. In such expression, IBs are often loose and of flocculate morphology. We believe that such distribution is due to association of near native structures of the expressed proteins, which cause either aggregati...
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PMID: 20210737
PDF is available here.
PDF is available here.
Abstract
Protein refolding from unfolded state is usually carried out at low temperature to reduce protein aggregation and proteolytic degradation. This review briefly introduces a unique method for the protein refolding via high temperature, typically at above melting temperature of the protein.
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PMID: 20210738
PDF is available here.
PDF is available here.
Abstract
We describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation....
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PMID: 20210742
PDF is available here.
PDF is available here.
Abstract
We therefore produced recombinant transcription factor proteins in E. coli (OCT4, SOX2, c-MYC and KLF4) carrying the cell penetrating TAT domain from HIV1. The purified proteins were able to enter into mammalian cells when added to tissue culture medium but appeared not to translocate to the nucleus...
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PMID: 19669668
PDF is available here.
PDF is available here.
Analytical Biochemistry (400)2 2010
Abstract
Human immunoglobulin G1 (IgG1) contains 12 domains, and each has an intrachain disulfide bond that connects the two layers of antiparallel beta-sheets. These intrachain disulfide bonds are shielded from solvents under native conditions. Therefore, accessibility of the disulfide bonds to reduction un...
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PMID: 20152794
PDF is available here.
PDF is available here.
Abstract
We have performed molecular dynamics simulations of the native state and unfolding pathways of over 2000 protein/peptide systems (approximately 11,000 independent simulations) representing the majority of folds in globular proteins. These data are stored and organized using an innovative database ap...
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PMID: 20399180
PDF is available here.
PDF is available here.
Abstract
We presented a review of our kinetic models for the nucleation mechanism of protein folding and for the protein thermal denaturation in a barrierless way. A protein was treated as a random heteropolymer consisting of hydrophobic, hydrophilic, and neutral beads. As a crucial idea of the model, an ove...
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PMID: 20163782
PDF is available here.
PDF is available here.
Biochemical Journal (425)3 2010
Abstract
Gram-negative bacteria have evolved diverse secretion systems/machineries to translocate substrates across the cell envelope. These various machineries fulfil a wide variety of functions but are also essential for pathogenic bacteria to infect human or plant cells. Secretion systems, of which there...
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PMID: 20070257
PDF is available here.
PDF is available here.
Abstract
These results demonstrate the usefulness of alkaline detergents and proteases for the degradation or destabilization of PrPSc....
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PMID: 20043137
PDF is available here.
PDF is available here.
Abstract
Abstract
We review recent endeavors to enhance metazoan proteostasis by applying Hsp104 to the specific protein-misfolding events that underpin two deadly neurodegenerative amyloidoses. Hsp104 potently inhibits Abeta42 amyloidogenesis, which is connected with Alzheimer's disease, but appears unable to disagg...
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PMID: 20130674
PDF is available here.
PDF is available here.
Abstract
We conducted an extensive computational analysis of the unfolding process for the monomeric form of YibK. In order to predict the (un)folding pathway of YibK, we have calculated the order of secondary structure disassembly using UNFOLD, and performed thermal unfolding simulations using classical Mol...
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PMID: 19916572
PDF is available here.
PDF is available here.
Abstract
The stability of camel alpha-lactalbumin (alpha-la) against heat denaturation was measured, using circular dichroism (CD) and fluorescence spectroscopy, as well as differential scanning calorimetry (DSC). The experiments were performed in the presence of saturating concentrations of calcium as well...
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PMID: 19930756
PDF is available here.
PDF is available here.