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Blastomeres (0):
Recent article
Blastomeres
Cellular Reprogramming (12)4 2010
Abstract
Universal recipients in the G2 phase of mitotic cell cycle (preactivated oocytes, zygotes, blastomeres) accept embryonic nuclei in all the stages of their cell cycle. To test if recipients in the G2 of meiotic cycle (immature oocytes) are universal recipients, mouse germinal vesicle (GV) oocytes wer...
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PMID: 20698781
PDF is available here.
PDF is available here.
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Prenatal Diagnosis (30)7 2010
Abstract
I and II, blastomere (8 cell embryo) and trophectoderm (5-6 day blastocyst). Diagnostic accuracy is very high (>99%) for both chromosomal abnormalities and single gene disorders. Traditional application of FISH with chromosome specific probes for detecting aneuploidy and translocations may be replac...
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PMID: 20572111
PDF is available here.
PDF is available here.
Abstract
Animal Reproduction Science (119)1-2 2010
Abstract
We used this method to examine 58 bovine embryos and the accuracy of sex prediction was 100% when the blastomeres dissociated from a morula exceeds three. This study showed that the present method can be applied in bovine breeding programs to facilitate manipulation of the sex ratio of offspring. Th...
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PMID: 20060664
PDF is available here.
PDF is available here.
Abstract
We focused on the derivation of human embryonic stem cell (hESC) from preimplantation genetic screening (PGS)-analyzed and preimplantation genetic diagnosis (PGD)-analyzed embryos. Out of 62 fresh PGD/PGS-analyzed embryos, 22 embryos reached the blastocyst stage. From 12 outgrowth blastocysts, we de...
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PMID: 20177993
PDF is available here.
PDF is available here.
Abstract
These results raise the possibility that accessory sperm cells may reduce the accuracy of the genetic diagnosis of bovine embryos. Therefore, steps to prevent the contamination of sperm cells, such as removal of the zona pellucida and washing of sample blastomeres, are necessary to obtain an accurat...
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PMID: 19881216
PDF is available here.
PDF is available here.
Abstract
We describe a protocol based on previous reports with modification published in Chiu et al. 2008....
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PMID: 20548282
PDF is available here.
PDF is available here.
Abstract
We demonstrate a protocol to generate chimeric retinas and brains in zebrafish embryos and to perform live imaging of the donor cells. The protocol covers the preparation of transplantation needles, the transplantation of GFP-expressing donor blastomeres to GFP-negative hosts, and the examination of...
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PMID: 20689504
PDF is available here.
PDF is available here.
Abstract
It has been shown that the isolation of sea urchin blastomeres before "pos-division adhesion" leads mainly to the formation of similar blastomeres at the stage of the 4th cleavage division, whereas after adhesion it results in the formation of micromeres simultaneously with intact embryos. Similar r...
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PMID: 20586324
PDF is available here.
PDF is available here.
Abstract
The results of pioneering studies on the development of radically new noninvasive methods for the transplantation of mammalian somatic cell nuclei with the use of optical laser manipulations are presented, and their comparison with traditional invasive methods is performed. It is shown that all the...
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PMID: 20586321
PDF is available here.
PDF is available here.
Abstract
We have investigated the expression patterns of 17 developmentally important genes and isoforms in early mouse embryos as well as in single cells of the mouse embryo. The comparison is an excellent example for showing the importance of studying heterogeneity among cell populations on the RNA level,...
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PMID: 20300987
PDF is available here.
PDF is available here.
Abstract
It remains controversial whether blastomeres of 2-cell stage mouse embryos show bias in their contribution to the blastocyst and whether there is any effect of superovulation. Two-cell stage embryos from CF1 mice were derived by either natural breeding (N) or superovulation (S) and cultured in vitro...
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PMID: 20209440
PDF is available here.
PDF is available here.
Abstract
Clinical pregnancy and ongoing pregnancy rates of frozen-thawed embryo transfer cycles were 35% and 30%, respectively. Higher pregnancy rates were associated with younger maternal age (<=35 years), blastomere numbers of 4 or more, and no blastomere lysis after thawing....
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PMID: 19966345
PDF is available here.
PDF is available here.
Abstract
We report that guanine nucleotide binding protein beta 2 (Gnb2; Gbeta2) bound to Axin and Gbeta2 inhibited Wnt mediated reporter activity. The inhibition involved reduction of the level of Dishevelled, and the Gbeta2gamma2 mediated reduction of Dishevelled was countered by increased expression of Ax...
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PMID: 19561403
PDF is available here.
PDF is available here.
Abstract
The purpose of this study was to investigate fertilization ability and embryo development to the blastocyst stage after reciprocal in vitro fertilization (IVF) between yak and cattle in an attempt to clarify the problem of low conception rate after mating yak females with cattle bulls. In vitro-matu...
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PMID: 19521055
PDF is available here.
PDF is available here.
Abstract
We have developed a single cell multiplex PGD protocol for nonsyndromic deafness with a high efficiency of diagnosis. Most PB1 are homozygous, and similar ADO rates were observed; therefore, blastomere biopsy appears to be the method of choice for this autosomal recessive disease....
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PMID: 19728075
PDF is available here.
PDF is available here.
Abstract
We report a novel method designated as "Separate and Seed" that contributes remarkably to efficient derivation of bovine primary ES-like cell colonies from blastocysts. These primary cultured bovine ES-like cells exhibit morphology typical of ES cells and express pluripotent molecular markers includ...
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PMID: 19340839
PDF is available here.
PDF is available here.
Abstract
We show that ectopic expression of the gamma-tubulin regulator, Shroom2, is sufficient to induce co-accumulation of pigment granules, spectrin, and dynactin in Xenopus blastomeres. Shroom2 and spectrin are enriched and co-localize specifically in the pigmented animal hemisphere of Xenopus eggs and b...
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PMID: 19554350
PDF is available here.
PDF is available here.
Abstract
Polyploid mouse embryos are important models for understanding the mechanisms of cleavage and preimplantation development in mammals. In this study, hexaploid (6n) mouse embryos were produced by the electrofusion of blastomeres from diploid (2n) and tetraploid (4n) embryos at the 2-cell stage. Furth...
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PMID: 19232146
PDF is available here.
PDF is available here.
Abstract
We used an integrase-defective lentiviral (IDLV) vector to decrease the chance of random integration and examined the feasibility of lentiviral vector-mediated gene targeting into murine embryonic stem (ES) cells. After transduction with wild-type lentiviral vectors, none of the 512 G418 resistant c...
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PMID: 19208434
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2009)4 2009
Abstract
One advantage of using Helobdella (leech) embryos as an experimental system is their amenability for microinjection. Blastomeres ranging in size from the zygote (400 microm diameter) down to micromeres and primary blast cells (approximately 20 microm diameter) can be injected by pres...
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PMID: 20147133
PDF is available here.
PDF is available here.
Fertility and Sterility (91)4 Suppl 2009
Abstract
The aim of this study was to determine the impact of day 3 embryo quality and quantity on subsequent embryonic development and pregnancy outcome following embryo biopsy. The findings demonstrate that a significantly lower proportion of poor/fair-quality embryos develop further after biopsy, and that...
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PMID: 18675415
PDF is available here.
PDF is available here.
Fertility and Sterility (91)4 Suppl 2009
Abstract
The blastomere biopsy procedure does not affect preimplantation embryo development or global patterns of gene expression in a mouse model of Preimplantation Genetic Testing (PGT). However, zona breaching, which is inherent to the blastomere biopsy procedure, causes significant premature and sometime...
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PMID: 18774571
PDF is available here.
PDF is available here.
Fertility and Sterility (91)3 2009
Abstract
OBJECTIVE: To analyze the genetic composition of oocytes and embryos presenting abnormal fertilization. DESIGN: Case report. SETTING: In vitro fertilization unit of a university-affiliated hospital. PATIENT(S): A couple with unexplained infertility with abnormal fertilizations in nine failed IVF-int...
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PMID: 19110241
PDF is available here.
PDF is available here.
Fertility and Sterility (91)3 2009
Abstract
OBJECTIVE: To determine the effects of sildenafil citrate, a cyclic monophosphate-specific type 5 phosphodiesterase inhibitor known to affect sperm function, on fertilization and early embryo cleavage. DESIGN: This acute mammal study included male and female mice assigned randomly, the females sacri...
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PMID: 18325509
PDF is available here.
PDF is available here.
Abstract
The great diversity of arthropod body plans, together with our detailed understanding of fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification of developmental patterns and hence the diversity of extant life. Crustaceans, in particular, show a remarka...
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PMID: 20147023
PDF is available here.
PDF is available here.
Abstract
These data corroborate conclusions made by evaluating single embryo transfer in women with diminished egg reserve that the presence of at least six blastomeres is associated with a better chance of a given embryo to implant...
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PMID: 19860351
PDF is available here.
PDF is available here.
Abstract
Though 4-cell embryos have a markedly lower implantation potential upon fresh embryo transfer compared to 6-8-cell embryos, this is not reflected in their ability to survive freeze-thawing....
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PMID: 20101846
PDF is available here.
PDF is available here.
Abstract
When given the option it is preferable when using this simplified freezing and thawing protocol to freeze at the 2PN stage....
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PMID: 20101847
PDF is available here.
PDF is available here.
Abstract
The changes in the state of long-term culture of blastocysts derived from female C57BL/6 mice after crossing with C57BL/6-Tg(ACTB-EGFP)1Osb/J males with a green fluorescent protein transgene EGFP on chromosome 15 were studied. Possible causes of different culture results were analyzed: the preservat...
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PMID: 19894611
PDF is available here.
PDF is available here.
Biology of Reproduction (80)1 2009
Abstract
We have developed a microfluidic chip-based qualitative assay for sensitive (10 RNA copies) detection of multiple transcripts in single cells. We determined the expression patterns of 17 developmentally important genes and isoforms in individual mouse preimplantation embryos from superovulated matin...
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PMID: 18784354
PDF is available here.
PDF is available here.
Reproductive BioMedicine Online (18 Suppl 1) 2009
Abstract
Polar body diagnosis (PBD) is a diagnostic method for the indirect genetic analysis of oocytes. Polar bodies are by-products of the meiotic cell cycle, which have no influence on further embryo development. The biopsy of polar bodies can be accomplished either by zona drilling or laser drilling with...
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PMID: 19281658
PDF is available here.
PDF is available here.
Abstract
In terms of treatment outcome, little prognostic power is attributed to day-4 morphology. A day-4 score was applied to 56 patients separating non-compacting embryos from compacting (some areas of compaction) and fully compacted embryos. The latter were further subdivided according to the morphology...
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PMID: 19146770
PDF is available here.
PDF is available here.
Abstract
We have identified SpolvlgA, a Schmidtea polychroa homolog of the DDX3/PL10 DEAD-box RNA helicase DjvlgA from the planarian species Dugesia japonica. This gene has been previously described as being expressed in planarian adult stem cells (neoblasts), as well as the germ line. Here we present the ex...
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PMID: 19159016
PDF is available here.
PDF is available here.
Abstract
We sought to resolve this conflict by manipulating egg size and food level during the development of two planktotrophic echinoid echinoderms: the green sea urchin, Strongylocentrotus droebachiensis and the sand dollar, Echinarachnius parma. Based on comparative datasets, we predicted that decreasing...
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PMID: 19878293
PDF is available here.
PDF is available here.
Abstract
These results suggest that administration of eFSH can increase the number of oocytes collected per cycle. Oocyte morphology differed with age and was associated with developmental competence....
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PMID: 19383268
PDF is available here.
PDF is available here.
Abstract
We analysed its distribution in the preimplantation mouse embryo. We found that H2A.X is highly phosphorylated throughout preimplantation development in the absence of any induced DNA damage. Moreover, gammaH2A.X levels vary significantly throughout the cell cycle. Interestingly, after the 4-cell st...
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PMID: 19598117
PDF is available here.
PDF is available here.
Abstract
This randomized, controlled trial verifies whether patients with recurrent failed implantation benefit from preimplantation genetic diagnosis for aneuploidy, as compared with conventional assisted reproduction treatment procedures. Two hundred patients with recurrent failed implantation were randomi...
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PMID: 19079970
PDF is available here.
PDF is available here.
Abstract
We ectopically expressed lamin A during early embryonic development of Xenopus laevis by microinjection of synthetic mRNA. Here, we show that introducing mature lamin A does not interfere with normal development. However, expression of prelamin A or lamin A variants that cannot be fully processed ca...
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PMID: 18675490
PDF is available here.
PDF is available here.
Genes & Development (22)19 2008
Abstract
We show that Cdx2 can also act earlier to influence cell allocation. Increasing Cdx2 levels in individual blastomeres promotes symmetric divisions, thereby allocating more cells to the trophectoderm, whereas reducing Cdx2 promotes asymmetric divisions and consequently contribution to the ICM. Furthe...
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PMID: 18832072
PDF is available here.
PDF is available here.
Abstract
We show here that OMA-1 and OMA-2, previously shown to regulate oocyte maturation, repress transcription in P0 and P1 by binding to and sequestering in the cytoplasm TAF-4, a component critical for assembly of TFIID and the pol II preinitiation complex. OMA-1/2 binding to TAF-4 is developmentally re...
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PMID: 18854162
PDF is available here.
PDF is available here.
Molecular Human Reproduction (14)10 2008
Abstract
The aim of this study was to validate the overall preimplantation genetic diagnosis (PGD)-PCR procedure and to determine the diagnostic value. Genotyped embryos not selected for embryo transfer (ET) and unsuitable for cryopreservation after PGD were used for confirmatory analysis. The PGD genotyped...
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PMID: 18805801
PDF is available here.
PDF is available here.
Swiss medical weekly : official journal of the Swiss Society of Infectious Diseases, the Swiss Society of Internal Medicine, the Swiss Society of Pneumology (138)37-38 2008
Abstract
As the destruction of viable developing embryos, even spare ones, raises serious ethical concerns, deriving hESC lines from arrested embryos may be an alternative approach to avoid embryo destruction. However, given the reduced derivation efficiency they should not be considered a unique and/or sele...
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PMID: 18803035
PDF is available here.
PDF is available here.
Prenatal Diagnosis (28)10 2008
Abstract
Objective
Accounting for possible recombinations in developing an accurate preimplantation genetic diagnosis (PGD) protocol based on familial haplotypes.
Methods
Haplotypes were constructed from genomic DNA in a family where the male was affected with tuberous sclerosis complex (TSC). Embryos were bi...
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PMID: 18792920
PDF is available here.
PDF is available here.
Theriogenology (70)4 2008
Abstract
We inferred that calyculin-A was useful for rapid induction of PCC, producing chromosome spreads suitable for cytogenetic analysis of blastomeres in G1 or G2/M phase of the cell cycle....
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PMID: 18556054
PDF is available here.
PDF is available here.
Abstract
We reviewed these new methods of hPSC derivation and addressed some of the problems associated with these advances. A brief summary of new advances in genome-wide chromatin immunoprecipitation-sequencing (ChIP-seq) technology and their implications for stem cell research were provided and discussed....
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PMID: 19112879
PDF is available here.
PDF is available here.
Fertility and Sterility (90)2 2008
Abstract
OBJECTIVE: To report a simplified embryo biopsy method for preimplantation genetic diagnosis (PGD). DESIGN: Technique and method. SETTING: A regional hospital in vitro fertilization (IVF) laboratory and private reproductive medicine clinic. PATIENT(S): Women undergoing IVF and PGD. INTERVENTION(S):...
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PMID: 17880956
PDF is available here.
PDF is available here.
Developmental Dynamics (237)8 2008
Abstract
We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA), free of either animal-derived or synthetic serum, and contains very low amounts of peptidic or proteinaceous materia...
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PMID: 18624284
PDF is available here.
PDF is available here.
Human Reproduction (23)8 2008
Abstract
Although based on a small number of embryos, we conclude that the blastomeres of a 4-cell stage human embryo are flexible and able to develop into blastocysts with ICM and TE....
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PMID: 18503052
PDF is available here.
PDF is available here.
Abstract
We have recently shown that adult nulliparous and multiparous hamster females show significant asynchrony and retard on early embryo development (from two blastomeres to morula stages) when compared with nulliparous young females. The number of cell-cell adhesions between blastomeres in early embryo...
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PMID: 18578949
PDF is available here.
PDF is available here.
Abstract
Laser-assisted hatching can improve the development of vitrified-warmed intact four-cell stage mouse embryos, whereas necrotic blastomere removal has no significant effect on the development of vitrified-warmed four-cell stage damaged embryos....
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PMID: 18688708
PDF is available here.
PDF is available here.
Abstract
Outcomes of transfer cycles with fully intact embryos (intact group) were compared with those in which all transferred embryos have lost one-two blastomeres (damage group). Comparable pregnancy rates (38.46% vs 38.64%), birth rates (34.62% vs 36.36%) and implantation rates (26.31% vs 26.25%) were ob...
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PMID: 18607715
PDF is available here.
PDF is available here.
Abstract
We conducted a series of experiments to distinguish between these possibilities. We showed that the shape of the ZP at the two-cell stage varied among embryos, ranging from near spherical to ellipsoidal, and that the ZP shape did not change until the blastocyst stage. In those embryos with an ellips...
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PMID: 18196554
PDF is available here.
PDF is available here.
Human Reproduction (23)7 2008
Abstract
A Day 4 scoring system was successfully developed and implemented. Day 4 SETs were found to be a viable option or alternative to Day 5 SETs with no difference in pregnancy rates....
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PMID: 18292597
PDF is available here.
PDF is available here.
Reproduction (135)6 2008
Abstract
We demonstrated the feasibility of establishing ES cells from a single BTM of outbred mice....
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PMID: 18502895
PDF is available here.
PDF is available here.
Abstract
We have previously indicated that porcine blastocysts can be produced by in vitro fertilization (IVF) and culture (IVC) in chemically defined porcine gamete medium (PGM) and porcine zygote medium (PZM)-5, respectively, In the present study, the effects of basic media and macromolecular components on...
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PMID: 18408352
PDF is available here.
PDF is available here.
Animal Reproduction Science (105)3-4 2008
Abstract
We generated bovine-ovine reconstructed embryos via iSCNT using bovine oocytes as recipient cytoplasm and ovine fetal fibroblast as donor cells. Chromosome composition, the total cell number of blastocyst and embryonic morphology were analyzed. In addition, mtDNA copy numbers both from donor cell an...
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PMID: 17399920
PDF is available here.
PDF is available here.
Endocrinology (149)5 2008
Abstract
We report defects to ovarian function associated with diet-induced obesity (DIO) that result in poor oocyte quality, subsequently reduced blastocyst survival rates, and abnormal embryonic cellular differentiation. To identify critical cellular mediators of ovarian responses to obesity induced insuli...
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PMID: 18276752
PDF is available here.
PDF is available here.
Abstract
These results showed that the three types of embryos had a similar tendency for mitochondrial change. Nevertheless, changes in GG embryos were more similar to changes in in vivo-produced embryos than were GC embryos, which had more extreme mitochondrial deviation. The results indicate the effects of...
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PMID: 18405430
PDF is available here.
PDF is available here.
Nature Genetics (40)4 2008
Abstract
We demonstrate Ezh2-independent targeting of maternally provided polycomb repressive complex 1 (PRC1) components to paternal heterochromatin. In Suv39h2 maternally deficient zygotes, PRC1 also associates with maternal heterochromatin lacking H3K9me3, thereby revealing hierarchy between repressive pa...
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PMID: 18311137
PDF is available here.
PDF is available here.