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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Cleavage Stage, Ovum (0):
Recent article
Cleavage Stage, Ovum
Developmental Cell (19)4 2010
Abstract
We show that pericentric satellites undergo a transient peak in expression precisely at the time of chromocenter formation. This transcription is regulated in a strand-specific manner in time and space and is strongly biased by the parental asymmetry. The transcriptional upregulation follows a devel...
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PMID: 20951352
PDF is available here.
PDF is available here.
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Abstract
Although selection of chromosomally normal embryos has the potential to improve outcomes for patients undergoing IVF, the clinical impact of aneuploidy screening by fluorescence in situ hybridization (FISH) has been controversial. There are many putative explanations including sampling error due to...
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PMID: 20479065
PDF is available here.
PDF is available here.
Zoological Research (31)4 2010
Abstract
We called it fusion cleavage, was observed, viz., before 2-cell, 4-cell, 8-cell blastomeres entered into next division, nuclei disappeared gradually, blastomere fused gradually into one cell. After some time, in the centre of the cell appeared gradually nuclei that had been doubled in number. The nu...
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PMID: 20740703
PDF is available here.
PDF is available here.
Human Reproduction (25)8 2010
Abstract
We have evaluated the effects of 10 ng/ml EGF in combination with 1 or 10 IU/ml of gonadotrophins (FSH/hCG 1:1 ratio) during in vitro maturation (IVM) of marmoset oocytes. Immature cumulus-oocyte complexes (COCs) were retrieved from ovarian antral follicles of unprimed monkeys. COCs from six animals...
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PMID: 20573678
PDF is available here.
PDF is available here.
Theriogenology (73)9 2010
Abstract
Modern society continuously exposes the population to electromagnetic radiation, the effects of which on human health, in particular reproduction, are still unknown. The aim of this research was to assess the effect of acute (1h) exposure of boar spermatozoa to a 50 Hz extremely low frequency electr...
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PMID: 20176397
PDF is available here.
PDF is available here.
Animal Reproduction Science (119)1-2 2010
Abstract
The possibility of inducing and synchronizing spawning can be very useful to facilitate fish farming, particularly in species that achieve ovarian development in captivity without ovulation occuring. The present study was undertaken to observe the morphological and normal embryonic development of th...
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PMID: 20079982
PDF is available here.
PDF is available here.
Animal Reproduction Science (119)1-2 2010
Abstract
The aim of the study was to determine a role of Hsp70 in the response of rabbit preimplantation embryos to hyperthermia (HT) in vitro. The embryos were cultured at standard (ST, 37.5 degrees C, control) or elevated (HT, 41.5 degrees C) temperature for 6h. In half of the embryos from both groups, Hsp...
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PMID: 20047804
PDF is available here.
PDF is available here.
Theriogenology (73)8 2010
Abstract
This investigation addresses the question whether it is possible to apply the open pulled straw (OPS) vitrification method, found to be effective for cryopreserving caprine (Capra aegagrus hircus) blastocysts, to other embryonal stages. Morulae, blastocysts and hatched blastocysts were cryopreserved...
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PMID: 20102780
PDF is available here.
PDF is available here.
Fertility and Sterility (93)7 2010
Abstract
OBJECTIVE: To establish a novel scoring system for human immature oocytes and estimate its prognostic effects on the in vitro maturation (IVM) and developmental potential of human immature oocytes. DESIGN: Prospective study. SETTING: Hospital-based IVF center. PATIENT(S): Patients with polycystic ov...
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PMID: 19249763
PDF is available here.
PDF is available here.
Development (137)10 2010
Abstract
We report that cell polarity and cell division are coordinated through an additional mechanism: the site of cell division repositions the PAR-2 boundary. Galpha-mediated microtubule-cortex interactions appear to direct cortical flows of PAR-2 and myosin toward the site of cell division, which acts a...
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PMID: 20430749
PDF is available here.
PDF is available here.
Biology of Reproduction (82)4 2010
Abstract
Early preimplantation embryos are sensitive to external osmolarity and use novel mechanisms to accumulate organic osmolytes and thus control their cell volumes and maintain viability. However, these mechanisms are restricted to the cleavage stages of development, and it was unknown whether postcompa...
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PMID: 20018907
PDF is available here.
PDF is available here.
Abstract
We show that medium conditioned by the stromal cell line PA6 (PA6-CM) can induce dopaminergic differentiation in neural stem cells (NSCs) derived from hESCs but not directly from hESCs, indicating that soluble factors produced by PA6 cells act at the NSC stage to specify a dopaminergic fate. To iden...
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PMID: 19788370
PDF is available here.
PDF is available here.
Abstract
We compared the kinetics of male and female embryo development and gene expression between male and female blastocysts. Bovine in vitro-matured oocytes (n = 8858) were fertilised with spermatozoa from each of three different bulls (X-sorted, Y-sorted or unsorted spermatozoa depending on the experime...
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PMID: 20047728
PDF is available here.
PDF is available here.
Abstract
This study investigates the suitability of surface-enhanced laser desorption and ionization time-of-flight (SELDI-TOF) and electrospray ionization (ESI) mass spectrometry for analysis of the proteins released by the mouse preimplantation embryo in vitro. SELDI-TOF analysis with CM10 or IMAC30 (but n...
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PMID: 20569467
PDF is available here.
PDF is available here.
Abstract
Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality. The evaluation of embryo quality based on morphological parameters is probably more predictive than zygote-score....
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PMID: 20579351
PDF is available here.
PDF is available here.
Abstract
Abstract
A significantly higher proportion of excellent quality embryos were observed in the early cleavage group compared to the late cleavage group (52.5 versus 28.9%, P < 0.01). In the early cleavage group there was also a higher rate of pregnancy per transfer compared with the late cleavage group (38.7 v...
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PMID: 19789972
PDF is available here.
PDF is available here.
Fertility and Sterility (91)3 2009
Abstract
OBJECTIVE: To determine the effects of sildenafil citrate, a cyclic monophosphate-specific type 5 phosphodiesterase inhibitor known to affect sperm function, on fertilization and early embryo cleavage. DESIGN: This acute mammal study included male and female mice assigned randomly, the females sacri...
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PMID: 18325509
PDF is available here.
PDF is available here.
Fertility and Sterility (91)3 2009
Abstract
OBJECTIVE: To determine the optimal number of day 3 embryos to transfer in women >or=38 years by conducting an evidence-based evaluation. DESIGN: Retrospective analysis of 2000-2004 national SART data. SETTING: National writing group. PATIENT(S): A total of 36,103 day 3 embryo transfers in women >or...
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PMID: 18321489
PDF is available here.
PDF is available here.
Abstract
The objective of this meta-analysis was to investigate the influence of meiotic spindle visualization in human oocytes on intracytoplasmic sperm injection (ICSI) outcomes. Search strategies included on-line surveys of databases (MEDLINE, EMBASE, Science Citation Index, Cochrane Controlled Trials Reg...
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PMID: 19192344
PDF is available here.
PDF is available here.
Abstract
This study examined the expression of human leukocyte antigen (HLA)-G and HLA-I (which includes HLA-A, -B, -C, -E and -F, but is without HLA-G) in the cleavage embryo and its supernatant, and related the results to embryo development including growth rate and grade. In total, 136 day-3 cleavage embr...
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PMID: 19192345
PDF is available here.
PDF is available here.
Tsitologiia (51)8 2009
Abstract
I with the method of indirect immunofluorescence was applied as an integrated approach to study localization of actin in the nuclei of two-cell mouse embryos. Monomeric actin and two oligomeric forms of actin were detected in the nuclei, and each of these forms demonstrated its own pattern of distri...
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PMID: 19799350
PDF is available here.
PDF is available here.
Abstract
We analysed its distribution in the preimplantation mouse embryo. We found that H2A.X is highly phosphorylated throughout preimplantation development in the absence of any induced DNA damage. Moreover, gammaH2A.X levels vary significantly throughout the cell cycle. Interestingly, after the 4-cell st...
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PMID: 19598117
PDF is available here.
PDF is available here.
Abstract
These results suggest that administration of eFSH can increase the number of oocytes collected per cycle. Oocyte morphology differed with age and was associated with developmental competence....
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PMID: 19383268
PDF is available here.
PDF is available here.
Abstract
We demonstrated that a 9-h heat shock of 42 degrees C can have marked stimulatory effects on porcine parthenogenetic embryo development if applied immediately after oocyte activation. Developmental discrepancies between heat-shocked (HS) and non-HS embryos were manifest as early as 3 h after activat...
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PMID: 19698293
PDF is available here.
PDF is available here.
Abstract
We can assume that the quality of re-expanded embryos does not differ between the two groups....
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PMID: 19698294
PDF is available here.
PDF is available here.
Abstract
We compared the effects of NaCl with those of concentrated solutions of two non-permeable osmotic agents, namely sucrose and trehalose, on the cryotolerance and developmental competence of porcine oocytes. In Experiment 1, porcine in vitro-matured cumulus-oocyte complexes (COCs; n = 1200) were expos...
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PMID: 19210925
PDF is available here.
PDF is available here.
Abstract
Human embryonic stem cells (hESC) hold great promise for use in regenerative medicine. However, the extraordinary potential of hESC as therapeutic tools is tempered by ethical, moral and political issues surrounding their derivation from human embryos. It has previously been proposed that ethical cr...
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PMID: 19192355
PDF is available here.
PDF is available here.
Abstract
These results suggest that ovarian stimulation parameter outcomes of GnRH antagonist cycles were not inferior to GnRH agonist cycles, therefore reduced embryo implantation and pregnancy rates in GnRH antagonist cycles can be attributable to possible deleterious effects on the endometrium....
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PMID: 19146771
PDF is available here.
PDF is available here.
Abstract
In terms of treatment outcome, little prognostic power is attributed to day-4 morphology. A day-4 score was applied to 56 patients separating non-compacting embryos from compacting (some areas of compaction) and fully compacted embryos. The latter were further subdivided according to the morphology...
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PMID: 19146770
PDF is available here.
PDF is available here.
Abstract
There was no difference in outcome parameters in 109 cycles where only Pattern "0" zygotes, according to our simplified pronuclear morphology classification, were transferred and 140 cycles where only "other" pattern zygotes were transferred, regardless of their cleavage rate. On the contrary, signi...
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PMID: 19192281
PDF is available here.
PDF is available here.
Abstract
In expanded-cumulus oocytes, the addition of leptin in IVM medium improved maturation (74% vs 44%, for 100 ng/ml leptin-treated and control groups, respectively; P < 0.05) and fertilization after ICSI (56% vs 23% for 10 ng/ml leptin-treated and control groups, respectively; P < 0.05). However, the d...
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PMID: 19835605
PDF is available here.
PDF is available here.
Fertility and Sterility (90)6 2008
Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection.
Abstract
OBJECTIVE: To evaluate efficiency of ionomycin on fertilization and cleavage rates, embryo development, and pregnancy rate after intracytoplasmic sperm injection (ICSI) in teratozoospermic patients. DESIGN: Laboratory study. SETTING: Isfahan fertility and infertility Center, and Royan institute, Teh...
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PMID: 18206146
PDF is available here.
PDF is available here.
Fertility and Sterility (90)6 2008
Abstract
We transferred 395 blastocysts to the uterine horn of 2.5-day pseudopregnant female mice. MAIN OUTCOME MEASURE(S): Cumulus expansion in human and mice oocytes, and two-cell rate, blastocyst rate, and delivered rate of live pups in mice. RESULT(S): In human and mouse oocytes, LIF induced cumulus expa...
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PMID: 18222433
PDF is available here.
PDF is available here.
Abstract
The present study was conducted to determine the criteria for selecting good quality embryos on Day-2 post-insemination and at the blastocyst stage. Bovine oocytes were matured, fertilized and cultured in vitro. First, Day-2 embryos were classified based on the number of blastomeres into 2-cell, 3-...
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PMID: 18957827
PDF is available here.
PDF is available here.
Abstract
We ectopically expressed lamin A during early embryonic development of Xenopus laevis by microinjection of synthetic mRNA. Here, we show that introducing mature lamin A does not interfere with normal development. However, expression of prelamin A or lamin A variants that cannot be fully processed ca...
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PMID: 18675490
PDF is available here.
PDF is available here.
Fertility and Sterility (90)5 2008
Abstract
I (M I) oocytes to the metaphase II (M II) stage. Germinal vesicle (GV) stage oocytes cultured with CC matured at a significantly higher rate than did GV oocytes cultured without CC. Although acceptable fertilization rates were obtained (62.1%), "usable" embryo yield was very low (13.0%). CONCLUSION...
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PMID: 17953965
PDF is available here.
PDF is available here.
Abstract
We analyzed the expression of four ZGA genes (hsp70.1, eif-1a, muerv and zscan4d) in embryos whose DNA replication was inhibited by treatment with aphidicolin, an inhibitor of DNA polymerase. Inhibiting the first round increased the expression levels of hsp70.1, eif-1a and zscan4d but decreased that...
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PMID: 18580041
PDF is available here.
PDF is available here.
Abstract
The objective of this study was to use mouse embryos as a model system to investigate the effect of co-culture of cumulus cells in Sydney IVF sequential media (Cook) on embryo development, based on the hypothesis that feeder cells in co-culture with a sequential medium could work synergistically to...
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PMID: 18560205
PDF is available here.
PDF is available here.
Abstract
Embryo quality is routinely evaluated based on morphological and kinematic criteria, but it should also be founded on clinical and biological data from the ovulation induction treatments and the data from the SPZ test, with, if necessary, their genetic analysis. Zygote quality evaluation is based on...
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PMID: 18786475
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to evaluate the stability of the aneuploidy rate of the first polar body. Knowing the stability of the oocyte aneuploidy rate for each patient would allow the first analysis to be used as a prognostic tool for further attempts at intracytoplasmic sperm injection (ICSI). Aft...
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PMID: 18681995
PDF is available here.
PDF is available here.
Abstract
Blastocyst-stage transfer has yielded excellent results in good prognosis IVF patients, but its efficacy in the general IVF population has not been clearly demonstrated. The objective of this study was to compare cleavage-stage and blastocyst-stage transfer in a mixed, general IVF population. In a p...
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PMID: 18681996
PDF is available here.
PDF is available here.
Abstract
The aim of this work was to characterize oocyte fertilization and embryo cleavage in nine AI bulls to find parameters suitable for prediction of in vitro fertility. According to the d8 blastocysts rate, they were categorized as high, medium and low productive (HP, MP and LP, mean: 25.4, 21.0 and 13....
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PMID: 18578953
PDF is available here.
PDF is available here.
Abstract
The vertebrate segmentation clock was identified 10 years ago as a molecular oscillator associated with the rhythmic production of embryonic somites. Since then, three major signaling pathways--Notch, FGF, and Wnt--have been shown to be activated periodically during segmentation and proposed to cons...
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PMID: 18625313
PDF is available here.
PDF is available here.
Abstract
We also compared the developmental process of VIVO embryos, in vitro-fertilized (IVF) embryos and nuclear transfer (NT) embryos obtained from enucleated oocytes and blastomere nuclei. The TCNs of embryos were measured using the air-dry method. Cleavage divisions (CD) of the embryos were obtained usi...
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PMID: 18441502
PDF is available here.
PDF is available here.
Abstract
The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to se...
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PMID: 18345029
PDF is available here.
PDF is available here.
Animal Reproduction Science (106)3-4 2008
Abstract
The objective of this study was to evaluate sperm functional attributes in relation to in vitro sperm-zona binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality. Frozen-thawed forty-eight ejaculates from eight Surti buffalo bulls (six ejaculates/bull) o...
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PMID: 17576042
PDF is available here.
PDF is available here.
Animal Reproduction Science (106)3-4 2008
Abstract
The effect of removing cytoplasmic lipid droplets (delipidation) at the 2-cell and developmental stages on the survival of porcine somatic cell nuclear-transferred blastocysts developed from the enucleated oocytes receiving somatic cells from kidney of an adult female after cryopreservation was exam...
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PMID: 17628361
PDF is available here.
PDF is available here.
Abstract
it has been observed that the presence of augmented first spindle pole is related to worse rates of fertilization, fragmentation and bad quality embryo formation. Nevertheless, fragmentation in the first spindle pole of the oocyte does not seem to affect ICSI results....
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PMID: 19142517
PDF is available here.
PDF is available here.
Reproduction (136)1 2008
Abstract
We used a mouse model to investigate the impact of a single, transient, mild, scrotal heat stress (38, 40 or 42 degrees C for 30 min) on testicular function, sperm DNA integrity and embryo survival. We detected temperature-dependent changes in testicular architecture, number of apoptotic cells and a...
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PMID: 18390691
PDF is available here.
PDF is available here.
Fertility and Sterility (90)1 2008
Abstract
OBJECTIVE: To investigate the characteristics of birefringence in human sperm heads and apply polarization microscopy for sperm selection at intracytoplasmic sperm injection (ICSI). DESIGN: Prospective randomized study. SETTING: Reproductive Medicine Unit, Società Italiana Studi Medicina della Ri...
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PMID: 18023436
PDF is available here.
PDF is available here.
Fertility and Sterility (90)1 2008
Abstract
OBJECTIVE: To examine the literature systematically in order to identify prospective comparative trials answering the following question: Is vitrification of human embryos associated with a higher postthawing survival rate as compared with slow freezing? DESIGN: Systematic review and meta-analysis....
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PMID: 17980870
PDF is available here.
PDF is available here.
Fertility and Sterility (90)1 2008
Abstract
Abstract
We examine the expression profiles of two new putative pluripotent stem cell genes, the embryo/cancer sequence A gene (ECSA) and the cancer/testis gene Brother Of the Regulator of Imprinted Sites (BORIS), in human oocytes, preimplantation embryos, primordial germ cells (PGCs) and embryo stem (ES) ce...
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PMID: 18467432
PDF is available here.
PDF is available here.
Reproduction (135)6 2008
Abstract
We demonstrated the feasibility of establishing ES cells from a single BTM of outbred mice....
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PMID: 18502895
PDF is available here.
PDF is available here.
Abstract
The results confirm that 25 h after insemination is a more effective critical time point for the selection of EC embryos and that the number of EC embryos could be a useful parameter for the prediction of multiple pregnancies....
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PMID: 18686354
PDF is available here.
PDF is available here.
Abstract
We identified human ePAB and ePABP2 cDNA. Human ePAB cDNA is not expressed specific to the ovary. Biological discrepancies exist between the human and the mouse....
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PMID: 18483763
PDF is available here.
PDF is available here.
Reproduction (135)5 2008
Abstract
We set out to examine the expression in human preimplantation embryos of a number of genes known to be critical for early development of the murine embryo. The expression profile of these genes was analysed throughout preimplantation development and in response to growth factor (GF) stimulation. Dev...
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PMID: 18411410
PDF is available here.
PDF is available here.
Reproduction (135)5 2008
Abstract
We attempted to control the nuclear remodelling of somatic cell nuclear transfer embryos (NTs) and examined their subsequent development and DNA methylation patterns in pigs. Porcine foetal fibroblasts were fused to enucleated oocytes treated with either 5 mM caffeine for 2.5 h or 0.5 mM vanadate fo...
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PMID: 18411411
PDF is available here.
PDF is available here.
Reproduction (135)5 2008
Abstract
We observed the interactive effects of cysteamine, cystine and cumulus cells on the glutathione (L-gamma-glutamyl-L-cysteinyl-glycine; GSH) level and developmental capacity of goat IVM oocytes. Cysteamine supplementation increased the GSH level and blastocyst rates of both cumulus-oocyte complexes (...
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PMID: 18411409
PDF is available here.
PDF is available here.