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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Morula (0):
Recent article
Morula
Abstract
We also suggest that the low hatching ability of the treated blastocysts is related to weak contractions with a low frequency and to strong contractions requiring a longer time for re-expansion....
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PMID: 19881218
PDF is available here.
PDF is available here.
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Abstract
The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment of whale iSCN...
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PMID: 19893277
PDF is available here.
PDF is available here.
Abstract
This study investigated the effect of donor cell types on the developmental potential and quality of cloned swamp buffalo embryos in comparison with cloned cattle embryos. Fetal fibroblasts (FFs), ear fibroblasts (EFs), granulosa cells (GCs) and cumulus cells (CCs) were used as the donor cells in bo...
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PMID: 19815984
PDF is available here.
PDF is available here.
Abstract
We analyzed the fertilization and embryonic development of the hybrid between spotted females and barred males of sorubim specimens by light microscopy and by scanning (SEM) and transmission electron microscopy (TEM) after induced spawning. Samples were collected at pre-established moments up to lar...
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PMID: 19876824
PDF is available here.
PDF is available here.
Abstract
This study aimed to investigate the effect of nerve growth factor (NGF) on the development of preimplantation rabbit embryos in vitro. Zygotes were collected from superovulated New Zealand rabbits 19 h after injection of hCG and immediately mating and cultured in TCM-199 plus fatty-a...
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PMID: 19834819
PDF is available here.
PDF is available here.
Abstract
Diazinon and malathion are commonly used organophosphate insecticides in agriculture, industry, and in veterinary medicine as an ectoparasiticide. The importance to carry out in vitro reproductive toxicology assays lies on the need of knowing the alterations these insecticides may cause at cellular...
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PMID: 19148769
PDF is available here.
PDF is available here.
Abstract
With an aim to isolate, culture and characterize goat embryonic stem cell-like cells derived from in vitro fertilized goat blastocysts, slaughterhouse derived goat oocytes were in vitro matured in maturation medium in 5% CO2 air at 38.5 degrees C. Matured oocytes were fertilized in vitro with fresh...
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PMID: 19775069
PDF is available here.
PDF is available here.
Methods in Molecular Biology (561)561 2009
Abstract
The previous two chapters have described the generation of chimeric embryos by blastocyst microinjection and morula aggregation. This chapter describes the reimplantation of these embryos into pseudopregnant female recipient mice (foster mice) in order for the embryos to develop to term. Four differ...
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PMID: 19504075
PDF is available here.
PDF is available here.
Theriogenology (71)9 2009
Abstract
We concluded that the modified droplet vitrification procedure supported better survival of morula stage compared to zygotes and 2-cell mouse embryos....
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PMID: 19268346
PDF is available here.
PDF is available here.
Animal Reproduction Science (112)1-2 2009
Abstract
We assessed whether co-transfer of parthenogenotes with a single embryo was sufficient to maintain development of the embryo. Embryos were recovered at morula and early blastocyst stages from gilts that had been artificially inseminated. Parthenogenotes were produced from oocytes matured in vitro, a...
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PMID: 18490116
PDF is available here.
PDF is available here.
Abstract
In evolution, the red panda (Ailurus fulgens) plays a pivotal role in the higher level phylogeny of arctoides carnivore mammals. The red panda inhabits certain Asian countries only and its numbers are decreasing. Therefore, the development of feasible ways to preserve this species is necessary. Gene...
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PMID: 19232145
PDF is available here.
PDF is available here.
Fertility and Sterility (91)3 2009
Abstract
OBJECTIVE: To develop sequential media for extended culture of embryos in human IVF, with use of a mouse embryo assay. The BlastAssist system was developed. Blastocyst culture has been our routine method since 1994, using earlier media IVF and M3 in sequence. DESIGN: Experimental laboratory study. S...
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PMID: 18321494
PDF is available here.
PDF is available here.
Abstract
Visible light adversely affects embryo development in a time-dependent manner. Feeder cells may enhance embryo development particularly when suboptimal conditions are involved....
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PMID: 19184398
PDF is available here.
PDF is available here.
Abstract
KCNJ11-encoded Kir6.2 assembles with ATP-binding cassette sulphonylurea receptors to generate ATP-sensitive K+ (KATP) channel complexes. Expressed in tissues with dynamic metabolic flux, these evolutionarily conserved yet structurally and functionally unique heteromultimers serve as high-fidelity rh...
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PMID: 18977736
PDF is available here.
PDF is available here.
Abstract
We analysed its distribution in the preimplantation mouse embryo. We found that H2A.X is highly phosphorylated throughout preimplantation development in the absence of any induced DNA damage. Moreover, gammaH2A.X levels vary significantly throughout the cell cycle. Interestingly, after the 4-cell st...
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PMID: 19598117
PDF is available here.
PDF is available here.
Abstract
This chapter describes the tools and the experimental route of targeted manipulation by aggregation in the mouse using targeted embryonic stem cells (ES cells). Instead of injecting ES cells into the blastocoel of a diploid blastocyst-stage embryo (3.5 dpc) ES cells can be brought together with dipl...
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PMID: 19504074
PDF is available here.
PDF is available here.
Abstract
These results suggest that administration of eFSH can increase the number of oocytes collected per cycle. Oocyte morphology differed with age and was associated with developmental competence....
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PMID: 19383268
PDF is available here.
PDF is available here.
Genetika (Moskva) (44)11 2008
Abstract
Twenty-eight independent extraembryonic endoderm (XEN) stem cell lines have been obtained from morula and blastocyst cells of common voles. Most cell lines form very few cell-cell contacts when growing and morphologically correspond to the XEN that were earlier described in mice. In addition, XEN ce...
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PMID: 19137730
PDF is available here.
PDF is available here.
Abstract
We observed round, yellow oocytes that bore a double chorion membrane and a single micropyle. At 10 s after fertilization, several spermatozoa were detected attached to the oocyte surface. After 1 min of development, a fertilization cone that obstructed the micropyle could be observed. Segmentation...
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PMID: 18616846
PDF is available here.
PDF is available here.
Experimental Cell Research (314)15 2008
Abstract
We have used interphase FISH to analyze the replication behavior of four imprinted chromosome regions (Snrpn, Zim1-Peg3, Dlk1-Gtl2, and Igf2r) and five non-imprinted regions in mouse one-cell to morula-stage embryos and embryonic fibroblasts. In general, imprinted chromosome regions showed the expec...
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PMID: 18675801
PDF is available here.
PDF is available here.
Abstract
The equivalence of the early mammalian cells, of importance in assisted reproductive technologies (ART), is considered. It is suggested that this controversial topic can be settled by finding whether the cells are distinguished by the Turing-Child (TC) field, as expressed for example by patterns of m...
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PMID: 18680762
PDF is available here.
PDF is available here.
Theriogenology (70)4 2008
Abstract
I actin were in intact (84%), Mi (71%) or VPII (70%) groups. A significantly lower number of embryos with grade I actin quality was observed in VPI (58%), VPI+Mi (54%) or VPII+Mi (66%). These observations indicate that of the vitrification schemes tested, the VPII using EG and ficoll 70 as cryoprote...
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PMID: 18539321
PDF is available here.
PDF is available here.
Reproduction (136)1 2008
Abstract
We used a mouse model to investigate the impact of a single, transient, mild, scrotal heat stress (38, 40 or 42 degrees C for 30 min) on testicular function, sperm DNA integrity and embryo survival. We detected temperature-dependent changes in testicular architecture, number of apoptotic cells and a...
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PMID: 18390691
PDF is available here.
PDF is available here.
Abstract
We have generated Aurora A null mouse embryos, which show severe defects at 3.5 d.p.c. (days post-coitus) morula/blastocyst stage and lethality before 8.5 d.p.c. Null embryos at 3.5 d.p.c. reveal growth retardation with cells in mitotic disarray manifesting disorganized spindle, misaligned and laggi...
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PMID: 18345035
PDF is available here.
PDF is available here.
Developmental Biology (318)1 2008
Abstract
We show that ZO-1 protein is first expressed during compaction of 8-cell embryos. This stage-specific appearance of ZO-1 suggests its participation in morula to blastocyst transition. Consistent with this idea, we demonstrate that ZO-1 siRNA delivery inside the blastomeres of zona-weakened embryos u...
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PMID: 18423437
PDF is available here.
PDF is available here.
Cryobiology (56)3 2008
Abstract
We tried to estimate the conditions in which ICI forms in vitrified mouse morulae during warming. The solutions for the experiments (EFS10-EFS50) contained 10-50% ethylene glycol plus Ficoll plus sucrose. When vitrified EFS20, EFS30, and EFS40 were kept at -80 degrees C, they remained transparent af...
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PMID: 18466891
PDF is available here.
PDF is available here.
Inheritance of histone H3 methylation in reprogramming of somatic nuclei following nuclear transfer.
Abstract
We transferred NIH3T3 cell nuclei into enucleated mouse oocytes and evaluated the histone H3 dimethyl-lysine 4 (H3K4me2) dynamics by immunocytochemistry. A low level of H3K4me2 in the somatic chromatin was maintained in pseudo-pronuclei. Unlike in vitro fertilized (IVF) embryos, the methylation leve...
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PMID: 18408353
PDF is available here.
PDF is available here.
Reproduction (135)5 2008
Abstract
We set out to examine the expression in human preimplantation embryos of a number of genes known to be critical for early development of the murine embryo. The expression profile of these genes was analysed throughout preimplantation development and in response to growth factor (GF) stimulation. Dev...
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PMID: 18411410
PDF is available here.
PDF is available here.
Reproduction (135)5 2008
Abstract
We use three-photon microscopy to image serotonin in live pre-implantation mouse embryos. We find that it is present as puncta averaging 1.3 square microns and in concentrations as high as 442 mM. The observed serotonin puncta were found to co-localize with mitochondria. Live embryos pre-incubated w...
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PMID: 18304982
PDF is available here.
PDF is available here.
Theriogenology (69)6 2008
Abstract
Oocytes from abattoir-derived bubaline (Bubalus bubalis) ovaries were subjected to IVM and IVF; the objective was to develop a pronuclear DNA microinjection technique to produce embryos expressing green fluorescent protein (GFP). The largest proportion (61.2%) of zygotes in which one (1 PN) or two p...
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PMID: 18272213
PDF is available here.
PDF is available here.
Pregnancy rate following transfer of in vitro produced lamb derived embryos in two embryonic stages.
Abstract
Ovine embryos were produced by maturation, fertilization and in vitro culture (IVM/IVF/IVC) of oocytes collected from slaughtered prepubertal ewes. At 24 h post IVM, oocytes were fertilized with fresh semen collected from Lori-Bakhtiari breed at a concentration of 1.0 x l0(6) sperm mL(-1). The presu...
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PMID: 18814661
PDF is available here.
PDF is available here.
Journal of Dairy Science (91)3 2008
Abstract
We conducted a field survey to estimate the relationship between embryo production and the body condition score (BCS) on a 5-point scale, as well as blood concentrations of insulin and glucose, in superovulated Holstein yearling heifers housed in a free-stall barn. They were provided total mixed rat...
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PMID: 18292264
PDF is available here.
PDF is available here.
Abstract
Murine 2-cells embryos were isolated from murine oviducts at laboratory and transferred into Ham's F-10 medium containing 0.1 mg mL(-1) streptomycin and 100 IU mL(-1) penicillin G and supplemented with 3 mg mL(-1) bovine serum albumin (BSA) or different concentrations of bovine follicular fluid (bFF...
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PMID: 18817138
PDF is available here.
PDF is available here.
Development (135)3 2008
Abstract
We examined whether PI3K is intrinsically activated during preimplantation development. Using GFP-tagged pleckstrin homology domains to monitor PtdIns(3,4,5)P(3) synthesis, we show that PI3K is constitutively activated in mouse preimplantation embryos. E-cadherin ligation promotes PtdIns(3,4,5)P(3)...
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PMID: 18094023
PDF is available here.
PDF is available here.
Abstract
The in vivo study showed that the rate of blastocyst implantation was not significantly different (9.2 vs 10.2 per mouse) and the rate of post-implantation embryo resorption was significantly higher in retinoic acid treated mice than in controls (35% vs 0%). RA was administered to morula-stage embry...
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PMID: 18419052
PDF is available here.
PDF is available here.
Abstract
Homozygous mutant embryos failed to develop beyond the morula stage, showing defective nucleologenesis, activation of apoptosis, and upregulation of P53 target genes. A unique feature of RBM19 is its localization to the cytoplasm in morula stage-embryos, whereas most other nucleolar proteins are loc...
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PMID: 19087264
PDF is available here.
PDF is available here.
Abstract
We examined the expression of ganglioside GT1b in the embryonic preimplantation stage after freezing and thawing processes to determine the regulatory roles of ganglioside GT1b in early embryonic development. ICR mouse embryos at the two-cell stage obtained by flushing the oviducts were frozen by tw...
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PMID: 18277613
PDF is available here.
PDF is available here.
Differentiation (76)1 2008
Abstract
We focus mainly on those events that take place soon after fertilization and during early embryogenesis in mammals. We will discuss the changes in DNA methylation and covalent histone modifications that were shown to be highly dynamic during this period; moreover, it has also been documented that ab...
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PMID: 18093226
PDF is available here.
PDF is available here.
Abstract
Retinoids regulate development and differentiation of the bovine blastocyst in vitro, although the underlying mechanisms remain to be clarified. A challenge in reproductive biotechnology is the identification of pathways that regulate early embryonic development and their influence on blastocyst dif...
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PMID: 19007552
PDF is available here.
PDF is available here.
Abstract
I summarize the last 10 years of research in this field.
2007 Wiley-Liss, Inc....
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PMID: 17893928
PDF is available here.
PDF is available here.
Abstract
These results indicate that the SP phenotype of ICM-derived ESCs is an intrinsic property and independent of the culture condition in vitro, and that SP phenotype is one of the characteristics of at least some pluripotent stem cells, but is not shared by totipotent stem cells. In addition, the resul...
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PMID: 17914640
PDF is available here.
PDF is available here.
Human Reproduction (22)8 2007
Abstract
Our results show that TE differentiation may be compromised by failure of membrane assembly of specific junction constituents. This abnormality relates more closely to metabolic profiles than morphological criteria. CONCLUSION: Our data identify that amino acid turnover can predict TE differentiatio...
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PMID: 17623723
PDF is available here.
PDF is available here.
Biology of Reproduction (77)2 2007
Abstract
We have shown in mouse morulae that water and glycerol move across the plasma membrane by facilitated diffusion, and we have suggested that aquaporin 3 plays an important role in their movement. In the present study, we clarify the contribution of aquaporin 3 to the movement of water and various cry...
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PMID: 17429015
PDF is available here.
PDF is available here.
Human Reproduction (22)7 2007
Abstract
To our knowledge, this is the first report describing development of cloned human embryos using IVM oocytes and non-autologous transfer using a conventional method of SCNT....
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PMID: 17513316
PDF is available here.
PDF is available here.
Reproduction (133)6 2007
Abstract
We showed that beta-adrenergic receptors can mediate the effects of catecholamines on preimplantation embryos in our previous work. In the present study, we designed specific oligonucleotide primers which can distinguish among all members of the alpha-adrenergic receptor family, and showed (using RT...
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PMID: 17636168
PDF is available here.
PDF is available here.
Reproduction (133)4 2007
Abstract
We related non-invasive parameters of oocyte and embryo quality, such as embryo kinetics, embryo morphology, and oocyte diameter, to the incidence of apoptosis throughout embryo development using fluorescent detection of active caspase-3 and -7. First, bovine in vitro embryos were selected according...
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PMID: 17504915
PDF is available here.
PDF is available here.
Abstract
Reproduction (133)2 2007
Abstract
We measured the mtDNA copy number and relative amount of mRNA for mitochondrial-related genes in individual rat oocytes, zygotes and embryos using real-time PCR. The average mtDNA copy number was 147 600 (+/-3000) in metaphase II oocytes. The mtDNA copy number was stable throughout in vivo early dev...
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PMID: 17307910
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to investigate the effects of different vitrification solutions [EFS30 or EFS40 contains 30% (v/v) ethylene glycol (EG), 40% (v/v) EG; EDFS30 or EDFS40 contains 15% (v/v) EG and 15% (v/v) dimethyl sulfoxide (DMSO), 20% (v/v) EG and 20% (v/v) DMSO], equilibrium time during v...
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PMID: 17214771
PDF is available here.
PDF is available here.
Abstract
We utilized microarray technology to compare gene expression profiles in four-cell (4C), morula (MO), and blastocyst (BL) stage embryos. Differences in spot intensities were normalized, and grouped by using Avadis Prophetic software platform (version 3.3, Strand Genomics Ltd.) and categories were ba...
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PMID: 16998848
PDF is available here.
PDF is available here.
Abstract
Our previous studies showed that expression patterns of X-linked genes in cultured cells are different from those of their tissues of origin. This investigation analyses the transcription pattern of the X-linked genes BIRC4, GAB3, MECP2, RPS4X, SLC25A6, and XIST in bovine in vitro matured oocytes an...
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PMID: 16998849
PDF is available here.
PDF is available here.
Theriogenology (67)2 2007
Abstract
We present here a procedure that is simple and reproducible for mass producing (10-150 embryos/vial/time) chimeric embryos by coculturing denuded 8-cell embryos and morula in 0.8 mL KSOM-AA medium containing 5 x 10(5)mL-1 purified green fluorescence protein-expressing ES cells (either fresh or thawe...
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PMID: 16987549
PDF is available here.
PDF is available here.
Abstract
In order to determine the effect of X-irradiation on intracellular signal transduction in mouse oocytes and embryos, JNK, ERK and p38 kinase activities were measured by the state of phosphorylation of their respective substrates (c-Jun, Elk-1 and ATF-2, respectively) in two mouse strains differing i...
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PMID: 17708350
PDF is available here.
PDF is available here.
Animal Biotechnology (18)1 2007
Abstract
These results demonstrate OPS method was highly efficient for the cryopreservation of mouse HBs....
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PMID: 17364443
PDF is available here.
PDF is available here.
Animal Biotechnology (18)1 2007
Abstract
For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution...
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PMID: 17364440
PDF is available here.
PDF is available here.
International Journal of Cancer (119)10 2006
Abstract
We focused on associations with alterations in p16(INK4A) and pRb expression during this process. In clinical cases, p16(INK4A) immunoreactivity was found to frequently overlap with nuclear beta-catenin accumulation in small-sized morules and surrounding glandular carcinomas (Sur-Ca), demonstrating...
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PMID: 16858682
PDF is available here.
PDF is available here.
Journal of Medical Ethics (32)11 2006
Abstract
The recent discussions about alternative sources of human embryonic stem cells (White Paper of the US President's Council on Bioethics, 2005), while stirring new interest in the developmental potential of the various abnormal embryos or constructs proposed as such sources, also raise questions about...
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PMID: 17074826
PDF is available here.
PDF is available here.
Abstract
We examined the preimplantation and postimplantation development of rat tetraploid embryos produced by electrofusion of 2-cell-stage embryos. Developmental rate of tetraploid embryos to morula or blastocyst stage was 93% (56/60) and similar to that found in diploid embryos (95%, 55/58). After embryo...
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PMID: 17266787
PDF is available here.
PDF is available here.
Biology of Reproduction (75)5 2006
Implication of nucleolar protein SURF6 in ribosome biogenesis and preimplantation mouse development.
Abstract
We examined the function of the evolutionary conserved nucleolar protein SURF6 in preimplantation mouse embryo development. Immunocytochemical analyses revealed that the localization of SURF6 was similar but not identical to those of fibrillarin and B23/nucleophosmin 1, which are involved in rRNA pr...
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PMID: 16855206
PDF is available here.
PDF is available here.
Animal Reproduction Science (95)3-4 2006
Abstract
Due to its economic importance, the production of cattle by nuclear transfer has been a primary research focus for many researchers during the past few years. While many groups have successfully produced cattle by nuclear transfer, and progress in this area continues, nuclear transfer remains a very...
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PMID: 16324805
PDF is available here.
PDF is available here.