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Integrases (1):
Recent article
Integrases
Developmental Cell (21)4 2011
Abstract
We use a combination of in vivo quantitative proteomics and analyses of cyclin E knockout mice to demonstrate that in terminally differentiated neurons cyclin E forms complexes with Cdk5 and controls synapse function by restraining Cdk5 activity. Ablation of cyclin E led to a decreased number of s...
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PMID: 21944720
PDF is available here.
PDF is available here.
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Abstract
We introduced the conditional ROSA26 luciferase reporter allele to monitor prostate cancer progression via bioluminescence imaging and extensively backcrossed mice onto the albino C57BL/6 genetic background to address variability in tumor kinetics and to enhance imaging sensitivity. Bioluminescence...
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PMID: 21703427
PDF is available here.
PDF is available here.
Proteomics (11)3 2011
Abstract
We have shown previously that the scaffold protein MEK1 partner (MP1) is localized to late endosomes by the adaptor protein p14. Using conditional gene disruption of p14 in livers of mice (p14(Δhep) ) we analyzed protein and transcript signatures in tissue samples. Further biological network analys...
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PMID: 21268275
PDF is available here.
PDF is available here.
Abstract
We utilized the N-terminal secretion signal sequence of Pseudomonas aeruginosa exotoxin ExoS to translocate Cre recombinase containing a nuclear localization sequence (Cre-NLS). Transient exposure of human sarcoma cell line, containing Cre-dependent lacZ reporter, resulted in efficient recombination...
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PMID: 21304583
PDF is available here.
PDF is available here.
Clinical calcium (21)6 2011
Abstract
Knockout mice are used to study physiological function of the gene, while transgenic mice expressing a high level of the transgene are used to examine pharmacological action of the gene. Genes can be deleted not only in chondrocytes, but also at various steps during the process of chondrocyte differe...
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PMID: 21628798
PDF is available here.
PDF is available here.
Abstract
We report crystal structures of the intasome from prototype foamy virus in complex with target DNA, elucidating the pre-integration target DNA capture and post-catalytic strand transfer intermediates of the retroviral integration process. The cleft between IN dimers within the intasome accommodates...
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PMID: 21068843
PDF is available here.
PDF is available here.
Abstract
We aimed to develop a protocol for tamoxifen-induced gene inactivation with minimal effects on gene transcription and in vivo cardiac function, allowing studies of acute loss of the targeted gene. In mRNA microarrays, 35% of the 34,760 examined genes were significantly regulated in MCM(+/0) compared...
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PMID: 20802136
PDF is available here.
PDF is available here.
Abstract
EMBO Journal (29)21 2010
CBP/p300 double null cells reveal effect of coactivator level and diversity on CREB transactivation.
Abstract
We show that the number of CREB-binding sites (CREs) affects whether the related histone acetyltransferases (HATs) CREB-binding protein (CBP) and p300 are required for endogenous gene transcription. Fibroblasts with both CBP and p300 knocked-out had strongly attenuated histone H4 acetylation at CREB...
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PMID: 20859256
PDF is available here.
PDF is available here.
Abstract
We describe the generation of an inducible Cre transgenic line allowing conditional mutagenesis in ovarian granulosa cells. We have expressed the tamoxifen inducible CreER(T)² fusion protein from a Bacterial Artificial Chromosome (BAC) containing the regulatory elements of the hydroxysteroid (17-be...
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PMID: 20715176
PDF is available here.
PDF is available here.
Abstract
We assessed whether TGF-alpha overexpression and TGF-beta signaling inactivation cooperate in hepatocarcinogenesis using an in vivo mouse model, MT1/TGFa;AlbCre/Tgfbr2(flx/flx) mice ("TGFa;Tgfbr2(hepko)"), which overexpresses TGF-alpha and lacks a TGF-beta receptor in the liver. TGF-beta signaling i...
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PMID: 20020490
PDF is available here.
PDF is available here.
Abstract
Retroviral integrases are vital enzymes in the viral life cycle and thus are important targets for antiretroviral drugs. The structure of the catalytic core domain of the integrase from human foamy virus, which is related to HIV-1, has been solved. The structure of the protein is presented in two di...
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PMID: 20693659
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
Retroviral integrases associate during the early viral life cycle with preintegration complexes that catalyze the integration of reverse-transcribed viral cDNA into the host chromosomes. Several cellular and viral proteins have been reported to be incorporated in the preintegration complex. This stu...
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PMID: 20519390
PDF is available here.
PDF is available here.
Abstract
We determine the lineages of adult hematopoietic cells derived from Tie2-expressing populations using Tie2-Cre;Rosa26R-EYFP mice. In Tie2-Cre;Rosa26R-EYFP mice, analysis of bone marrow cells showed Cre-mediated recombination in 85% of the population. In adult bone marrow and spleen, we analyzed subc...
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PMID: 20645309
PDF is available here.
PDF is available here.
Abstract
We have previously generated a transgenic mouse strain (LSL-TβRI(CA)) containing a Cre-inducible constitutively active TGFβ type I receptor (Bartholin et al., 2008, Genesis 46: 724-731). Transgene expression depends on the excision of a floxed-transcriptional STOP (LSL, Lox-STOP-Lox) located upstr...
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PMID: 20645310
PDF is available here.
PDF is available here.
Abstract
We show that an LTR retrotransposon of ERV-9 human endogenous retrovirus located 40-70 kb upstream of the human fetal gamma- and adult beta-globin genes serves a long-range, host function. The ERV-9 LTR contains multiple CCAAT and GATA motifs and competitively recruits a high concentration of NF-Y a...
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PMID: 20615953
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We investigated ischemia-induced revascularization in mice that were deficient for LKB1 in Tie2-Cre-expressing cells. Whereas homozygous deletion of LKB1 led to embryonic lethality, heterozygous LKB1-knock-out (KO) (Lkb1(flox/+);Tie2(Tg/+)) mice were viable. Unchallenged heterozygous LKB1-KO mice di...
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PMID: 20489196
PDF is available here.
PDF is available here.
Abstract
We have generated a transgenic mouse line in which two conserved exons of the mouse intermediate dynein chain gene, Dnaic1, are flanked by loxP sites (Dnaic1(flox/flox)). Dnaic1 is the murine homolog of human DNAI1, which is mutated in approximately 10% of human PCD cases. These mice have been cross...
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PMID: 19675306
PDF is available here.
PDF is available here.
Abstract
We report the generation and initial characterization of a mouse line expressing tamoxifen-inducible improved Cre (iCre) recombinase (iCre-ER(T2)) under the regulation of NPHS2 (podocin) gene promoter. The resulting transgenic mouse line was named podocin-iCreER(T2) mice. The efficiency of iCre acti...
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PMID: 20641128
PDF is available here.
PDF is available here.
Cell Stem Cell (6)6 2010
Abstract
We have used Cre-lox fate mapping in transgenic mice to show that PDGFRA/NG2-expressing glia, a distributed population of stem/progenitor cells in the adult CNS, produce the remyelinating oligodendrocytes and almost all of the Schwann cells in chemically induced demyelinated lesions. In contrast, th...
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PMID: 20569695
PDF is available here.
PDF is available here.
Abstract
Genetic immunization with lentiviral vectors is under evaluation as a means for induction of sustained immune response. Lentiviral vectors showed reduced antivector immune responses and efficiently transduce post-mitotic cells in vivo, including antigen presenting cells, such as dendritic cells and...
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PMID: 20353396
PDF is available here.
PDF is available here.
Abstract
Abstract
In order to study biochemical properties, the integrase (IN) protein of feline foamy virus (FFV) was over-expressed from Escherichia coli, purified by two-step chromatography; Talon column and heparin column, and characterized in biochemical aspects. For the three enzymatic reactions of the 3' -proc...
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PMID: 20622493
PDF is available here.
PDF is available here.
Nucleic Acids Research (38)11 2010
Abstract
We show here that the frequency of cassette exchange is dependent on the relative and absolute quantities of the transgene cassette and the recombinase. We were able to successfully modify genomic targets not only by electroporation or chemically mediated gene transfer but also by using an adenoviru...
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PMID: 20371519
PDF is available here.
PDF is available here.
Human Gene Therapy (21)5 2010
Abstract
We therefore developed a direct integrase fusion protein strategy as an alternative way to package heterologous proteins into vectors. The method was tested by creating two different integrase fusion proteins, IN-p53 and IN-mCherry, cloned into the 3' end of pol in the packaging plasmid. Lentiviral...
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PMID: 20039782
PDF is available here.
PDF is available here.
Abstract
Purpose. To investigate the specific role of Pinin (Pnn) in the development of anterior eye segment in mice. Methods. Conditional inactivation of Pnn in the developing surface eye ectoderm and lens was achieved by creating mice carrying a Pnn null and a floxed Pnn allele as well as a Pax6-Cre-GFP (L...
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PMID: 19892877
PDF is available here.
PDF is available here.
Abstract
Most temperate phages encode an integrase for integration and excision of the prophage. Integrases belong either to the lambda Int family of tyrosine recombinases or to a subgroup of the serine recombinases, the large serine recombinases. Integration by purified serine integrases occurs efficiently...
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PMID: 20298189
PDF is available here.
PDF is available here.
Methods in Molecular Biology (614)614 2010
Abstract
Lentiviral vectors are a powerful tool for gene transfer into target cells in vitro and in vivo. However, there are concerns about safety with regard to their use in gene transfer protocols because of insertional mutagenesis following viral infection. Once in the target cells, and in addition to the...
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PMID: 20225038
PDF is available here.
PDF is available here.
Hypertension (55)3 2010
Abstract
We recently found that endogenous (free fatty acids) and pharmacological (thiazolidinediones) agonists of nuclear receptor Peroxisome proliferator-activated receptor (PPAR)gamma stimulate renin transcription. In addition, the renin gene was identified as a direct target of PPARgamma. The mouse renin...
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PMID: 20065157
PDF is available here.
PDF is available here.
Abstract
We have adapted the Bacteriophage P1 Cre-loxP system for use in C. neoformans and successfully excised and reused the same drug marker, G418, to make two sequential gene deletions, lac1Delta and cap59Delta, in the same strain. This tool will allow investigators to make multiple sequential gene delet...
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PMID: 19944774
PDF is available here.
PDF is available here.
Abstract
Abstract
We tested the feasibility, effectiveness, and cell specificity of inducible Cre-mediated gene recombination in the postnatal inner ear using mice that express an inducible form of Cre (CreER(T)) under the transcriptional control of the proteolipid protein (PLP) promoter. We assessed the pattern of t...
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PMID: 19820996
PDF is available here.
PDF is available here.
Abstract
We generated transgenic mouse line C57BL/6-Tg(Hspa2-cre)1Eddy/J (Hspa2-cre), which expresses cre-recombinase under the control of a 907-bp fragment of the heat shock protein 2 (Hspa2) gene promoter. Transgene expression was determined using Gt(ROSA)26Sor(tm1Sor)/J (ROSA26) and Tg(CAG-Bgeo/GFP)21Lbe/...
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PMID: 20027617
PDF is available here.
PDF is available here.
Abstract
We generated a mouse model that enables conditional ablation of PR function in a spatiotemporal specific manner. Exon 2 of the murine PR gene was floxed to generate a conditional PR allele (PR(flox)) in mice. Crossing the PR(flox/flox) mouse with the ZP3-cre transgenic demonstrated that the PR(flox)...
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PMID: 20029965
PDF is available here.
PDF is available here.
Endocrinology (151)2 2010
Precursor cells in mouse islets generate new beta-cells in vivo during aging and after islet injury.
Abstract
We tested whether pancreatic islets of adult mice contain precursor beta-cells that initiate insulin synthesis during aging and after islet injury. We used bigenic mice in which the activation of an inducible form of Cre recombinase by a one-time pulse of tamoxifen results in the permanent expressio...
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PMID: 20056825
PDF is available here.
PDF is available here.
BioTechniques (48)2 2010
Abstract
We have established an in vitro Cre/loxP-based assay for monitoring cell fusion events that specifically traces the transport of cytoplasm from one cell to its fusion partner. Cells with a double fluorescence vector indicate fusion with cells expressing Cre recombinase by switching expression from r...
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PMID: 20359294
PDF is available here.
PDF is available here.
Abstract
The current system (A + B + C) demonstrated significantly better cytotoxic effect for CEA-producing cell lines than did suicide gene therapy (A + B) at the same viral dose in vitro. The effect of oncolytic suicide gene therapy was almost equal to that of the tenfold viral dose of suicide gene therap...
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PMID: 20012217
PDF is available here.
PDF is available here.
Gene Therapy (17)2 2010
Abstract
We show by immunofluoresence that wild-type phiC31 integrase is cytoplasmic and that addition of the SV40 nuclear localization signal (NLS) localized phiC31 integrase to the nucleus. Unexpectedly, the NLS depressed integration efficiency in HeLa cells and provided no benefit when used to integrate t...
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PMID: 19847205
PDF is available here.
PDF is available here.
Abstract
We therefore conducted an extensive in silico study of class 1 integron sequences in order to assess the distribution of Pc variants. We also measured these promoters' strength by means of transcriptional reporter gene fusion experiments and estimated the excision and integration activities of the d...
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PMID: 20066027
PDF is available here.
PDF is available here.
Abstract
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology i...
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PMID: 19966808
PDF is available here.
PDF is available here.
Abstract
We demonstrate the use of an adenovirus containing the Cre recombinase gene to infect primary mouse embryonic fibroblasts (MEFs) obtained from embryos containing a floxed Rac1 allele. Our rationale for selecting Rac1 MEFs for our experiments is that clear morphological changes can be seen upon delet...
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PMID: 20864926
PDF is available here.
PDF is available here.
Abstract
Integrons gained great interest due to their participation in resistance gene recruitment and expression. Their basic structure includes a fragment that encodes an integrase (intI) followed by a recognition sequence (attI) into which they may incorporate gene cassettes (encoding resistance mechanism...
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PMID: 20461298
PDF is available here.
PDF is available here.
médecine/sciences (26)1 2010
Abstract
Nature Neuroscience (13)1 2010
Abstract
We have generated a set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra. The robust native fluorescence of these reporters enables direct visualization of fine dendritic structures and axonal projections of the labeled neurons, which is useful in...
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PMID: 20023653
PDF is available here.
PDF is available here.
BMC Biotechnology (10) 2010
Abstract
We therefore evaluated the utility of Real-Time PCR to measure 1lox/2lox allele ratios.
We show that SYBR Green based Real-Time PCR analysis of 1lox/2lox allele ratios can generate erroneous peaks in the melting curve that are possibly caused by alternate hybridization products promoted by the palin...
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PMID: 20950424
PDF is available here.
PDF is available here.
Abstract
In this study, we investigated the potential benefits of overall MEF2 inhibition in a mouse model of chronic pressure overloading, by subjecting transgenic mice expressing a dominant negative form of MEF2 (DN-MEF2 Tg) in the heart, to transverse aortic constriction (TAC). Histological analysis revea...
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PMID: 20023039
PDF is available here.
PDF is available here.
BMC Biotechnology (10) 2010
Abstract
Using ES cells, we compared six RE mutant lox sites, focusing on their recombination efficiency with lox71. All of the RE mutant lox sites showed similar recombination efficiency. We then analyzed the stability of the recombined product, i.e., the LE+RE double mutant lox site, under continuous and s...
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PMID: 20356367
PDF is available here.
PDF is available here.
Abstract
We developed a method for delivering RU486, the conditional activator of Cre recombinase progesterone receptor (CrePR) fusion proteins to the lung and then examined the activity of three keratin-driven CrePR constructs in the conducting airways. We also developed a technique for survival bronchioalv...
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PMID: 20140084
PDF is available here.
PDF is available here.
BMC Microbiology (10) 2010
Abstract
The system established in this study allows us to express an N-terminal epitope tagged gene from its own endogenous promoter in Tetrahymena....
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PMID: 20626890
PDF is available here.
PDF is available here.
Abstract
We describe recombination-induced tag exchange (RITE), a genetic method that induces a permanent epitope-tag switch in the coding sequence after a hormone-induced activation of Cre recombinase. The time-controlled tag switch provides a unique ability to detect and separate old and new proteins in ti...
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PMID: 20018668
PDF is available here.
PDF is available here.
Abstract
We have developed a fast and efficient site-specific system of integrative recombination into the P. falciparum genome, which is catalyzed by the mycobacteriophage Bxb1 serine integrase. This system has the advantage of providing greater genetic and phenotypic homogeneity within transgenic lines as...
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PMID: 20676977
PDF is available here.
PDF is available here.
Journal of Virology (84)1 2010
Abstract
We correlate infectivity defects in transportin 3 knockdown cells with in vitro protein binding affinities for an expanded set of retroviruses that include simian immunodeficiency virus (SIV), bovine immunodeficiency virus (BIV), equine infectious anemia virus (EIAV), feline immunodeficiency virus (...
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PMID: 19846519
PDF is available here.
PDF is available here.
Molecular Pain (6)1 2010
Abstract
We deleted ephrin-B2 in Nav1.8+ nociceptive sensory neurons with the Cre-loxP system. Sensory neuron numbers and terminals were examined using neuronal makers. Pain behavior in acute, inflammatory and neuropathic pain models was assessed in the ephrin-B2 conditional knockout (CKO) mice. We also inve...
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PMID: 21059214
PDF is available here.
PDF is available here.
Molecular Pain (6)1 2010
Abstract
We deleted ephrin-B2 in Nav1.8+ nociceptive sensory neurons with the Cre-loxP system. Sensory neuron numbers and terminals were examined using neuronal makers. Pain behavior in acute, inflammatory and neuropathic pain models was assessed in the ephrin-B2 conditional knockout (CKO) mice. We also inve...
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PMID: 21059214
PDF is available here.
PDF is available here.
Abstract
These results provide evidence for cell specific post integrative gene silencing with C31 integrase and demonstrate the pivotal role of donor plasmid in long-term expression attained with this system....
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PMID: 19745601
PDF is available here.
PDF is available here.
Journal of Antibiotics (62)12 2009
Abstract
I and MK1688 were investigated in vitro against human immunodeficiency virus type-1 (HIV-1) integrase and Moloney murine leukemia virus reverse transcriptase. BEA, EN I and EN MK1688 exhibited strong inhibitory activities against HIV-1 integrase, whereas EN H showed relatively weak activity. None of...
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PMID: 19893585
PDF is available here.
PDF is available here.
Abstract
I and class II integron detected in 43 and 3 A. baumannii isolates, respectively. Twenty-seven of 52 clinical and 19 of 44 environmental isolates were integron-positive. Detection rate of integron-positive A. baumannii isolated from VAP patients increased from 25% to 83% over the 4 month study perio...
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PMID: 20578463
PDF is available here.
PDF is available here.
Abstract
We have analyzed the ability of these integrases to mediate intramolecular integration between their attB and attP sites in 7 important insect cell lines as a means of predicting their relative mobility in the corresponding insect species. Both integrases exhibit significantly higher frequencies in...
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PMID: 20578458
PDF is available here.
PDF is available here.
Abstract
We labeled two populations of beta-cells within the developing zebrafish pancreas. These populations originate in distinct pancreatic buds and exhibit gene expression profiles suggesting distinct functions during development. We find that the dorsal bud derived beta-cells are quiescent and exhibit a...
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PMID: 19706417
PDF is available here.
PDF is available here.
Developmental Biology (332)1 2009
Abstract
We developed MyoD(iCre) knockin mice, and used Cre/lox lineage analysis to determine whether satellite cell progenitors express MyoD, a marker of myogenic commitment. Recombination status of satellite cells was determined by confocal microscopy of isolated muscle fibers and by electron microscopic o...
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PMID: 19464281
PDF is available here.
PDF is available here.