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Culture Media (2):
Recent article
Culture Media
Abstract
We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises...
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PMID: 21998392
PDF is available here.
PDF is available here.
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Abstract
Our results showed that human urine increased the infectivity and proliferation of Leishmania parasites....
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PMID: 21976564
PDF is available here.
PDF is available here.
Abstract
We show that this QS system plays a major role in the control of S. pneumoniae biofilm formation. Our results demonstrate that the luxS gene is contained by invasive isolates and normal-flora strains in a region that contains genes involved in division and cell wall biosynthesis. The luxS gene was m...
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PMID: 21825061
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)10 2011
Abstract
Although Bioglass® has existed for nearly half a century its ability to trigger bone formation and tuneable degradability is vastly superior to other bioceramics, such as SiO(2)-CaO bioactive glasses. The sol-gel process of producing glass foams is well established for SiO(2)-CaO co...
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PMID: 21689791
PDF is available here.
PDF is available here.
Abstract
In vitro asymbiotic seed germination potential of its immature seeds (36 weeks after pollination) of G. calceolaris was successfully tested on three different agar gelled nutrient media i.e. Murashige and Skoog (MS), Mitra et al. (M) and potato dextrose agar (PDA). Seeds germinated w...
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PMID: 21941946
PDF is available here.
PDF is available here.
Abstract
We investigated the delivery of vascular endothelial growth factor (VEGF) and the effect on in vitro cultured human umbilical vein endothelial cells (HUVECs), in an attempt to provide experimental basis for promoting the vascularization of tissue-engineered bone grafts. A mechanical stimulator was d...
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PMID: 21341993
PDF is available here.
PDF is available here.
Abstract
We show that AFCs express pluripotency markers (Nanog, Sox2, and Oct3/4) as well as retinal transcription factor genes (Et, Lhx2, Tll1, Six6, Otx2, Pax6, and Fgf15). AFCs from amniotic fluid of Fgf15.gfp, Nrl.gfp, and Crx.gfp embryos cultured in retinal proliferation and differentiation conditions f...
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PMID: 20939691
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Immunoglobulins are a heterogeneous group of proteins. It naturally follows that the strategies for purifying them are diverse and numerous. A good knowledge of their respective physiochemical properties will obviously make the task easier. The choice between using polyclonal and/or monoclonal antib...
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PMID: 18612609
PDF is available here.
PDF is available here.
Abstract
Keratinocyte culture medium (KCM) has been used for the in vitro culture of keratinocytes and other types of epithelial cells, and the medium includes various ingredients. In this study, two modified KCMs were prepared. In the first, insulin, hydrocortisone and antibiotics that are n...
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PMID: 20740688
PDF is available here.
PDF is available here.
Abstract
A possible role of metabolism by intestinal bacteria in arbutin-induced toxicity was investigated in mammalian cell cultures. Following an incubation of arbutin with intestinal bacteria, either Bifidobacterium longum HY81 or Bifidobacterium adolescentis, for 24 h, its aglycone hydroquinone could be p...
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PMID: 21544735
PDF is available here.
PDF is available here.
Abstract
High-level production of α-cyclodextrin glycosyltransferase (CGTase) is one of the key factors in α-cyclodextrin (CD) preparation. In the present study, a fed-batch fermentation strategy for high-cell-density cultivation of Escherichia coli and the extracellular production of recombinant α-CGTase...
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PMID: 21417392
PDF is available here.
PDF is available here.
Abstract
The environmental significance of enantioselectivity in chiral insecticides and herbicides has been widely studied. However, little information is currently available on the enantioselective behavior of chiral plant growth regulators. In this study, paclobutrazol enantiomers were resolved and prepare...
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PMID: 21395310
PDF is available here.
PDF is available here.
Abstract
Cedrela odorata (Meliaceae) is considered as one of the most valuable forest tree in the tropics. Clonal propagation of this species provide an alternative method to propagate superior genotypes, being the production of good quality adventitious roots one of the most important steps in micropropagati...
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PMID: 21513204
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to assess the cytotoxicity of orthodontic materials (brackets, wires, resin, elastomers and silver solder) using Saccharomyces cerevisiae as a model organism. The induction of cytotoxicity was assessed by two different tests using the wild-type S. cerevisiae strain FF18733:...
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PMID: 21376384
PDF is available here.
PDF is available here.
médecine/sciences (27)3 2011
Abstract
Mesenchymal stem cells (or stromal cells) have been initially characterized in bone marrow, but since, they have been identified in almost every tissue. Their multiple properties, namely differentiative capacity, production of cytokines and trophic molecules, and their immunosuppressive potential und...
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PMID: 21447303
PDF is available here.
PDF is available here.
Abstract
Streptococcus agalactiae is a significant worldwide cause of morbidity and mortality in pregnant women and their newborn infants. The objective of this work was to determine the usefulness of bioMrieux chromogenic medium chromID Strepto B (CR) for detecting S. agalactiae in pregnant women from the se...
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PMID: 21491059
PDF is available here.
PDF is available here.
Abstract
Maternal diabetes adversely impacts embryonic vasculogenesis, which results in embryonic vasculopathy. The purpose of our study is to determine whether hypoxia inducible factor (HIF)-1α plays a role in diabetic embryonic vasculopathy.
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PMID: 21345401
PDF is available here.
PDF is available here.
Abstract
The potential of laser-induced breakdown spectroscopy (LIBS) to discriminate biological and chemical threat simulant residues prepared on multiple substrates and in the presence of interferents has been explored. The simulant samples tested include Bacillus atrophaeus spores, Escheri...
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PMID: 21331489
PDF is available here.
PDF is available here.
Abstract
Sexual Health (8)1 2011
Abstract
The use of nucleic acid amplification tests (NAAT), as well as or in preference to culture for non-genital sites is now recommended both in Australia and overseas because of their greater sensitivity and improved specificity. A survey of 22 Australian sexual health clinics who each year test over 145...
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PMID: 21371376
PDF is available here.
PDF is available here.
Abstract
Bioactive lipids derived from polyunsaturated fatty acids constitute an important set of lipid mediators with diverse homeostatic and pathophysiological activities. The role of bioactive lipids in inflammation, active resolution of inflammation, reproduction, cardiovascular biology, chronic diseases...
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PMID: 21236355
PDF is available here.
PDF is available here.
Abstract
Abstract
The objective of the present study was to develop a nanocarrier based formulation of nadifloxacin and to investigate its in vitro antimicrobial effect against Propionibacterium acnes. Nanocarrier based microemulsion formulations were prepared by aqueous titration method, using oleic...
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PMID: 21434572
PDF is available here.
PDF is available here.
Abstract
In the search for bioactive compounds, 11 fungal strains were isolated from Indonesian marine habitats. Ethyl acetate extracts of their culture broth were tested for cytotoxic activity against a urinary bladder carcinoma cell line and for antifungal and antibacterial activities again...
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PMID: 21556160
PDF is available here.
PDF is available here.
Abstract
This study provides an efficient method for obtaining a high-yield of EBECs and for generating highly differentiated cultures. These EBEC cultures can be used to study the formation of tight junction or to identify epithelial-derived inflammatory factors that contribute to lung diseases such as asth...
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PMID: 21649893
PDF is available here.
PDF is available here.
Abstract
We developed a basal media containing insulin and heparin. In this media, BMP4 induction of TE was dose dependent and with activin A inhibition by SB431542 (SB), approached 100% of cells. This paracrine stimulation of pluripotent cells transformed colony morphology from a cuboidal to squamous epithe...
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PMID: 21204619
PDF is available here.
PDF is available here.
Abstract
We have previously suggested, based on Small Angle X-ray Scattering data, that recombinant murine proNGF has features typical of an intrinsically unfolded protein. Using complementary biophysical techniques, we show here new evidence that clarifies and widens this hypothesis through a detailed compa...
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PMID: 21818348
PDF is available here.
PDF is available here.
Abstract
We developed an in vitro protein-based assay system for the rapid detection and quantification of the furanosyl borate diester (BAI-2) subclass of autoinducer-2 (AI-2) QS molecules. The biosensor is based on the interaction of BAI-2 with the Vibrio harveyi QS receptor LuxP. Conformation changes asso...
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PMID: 21031302
PDF is available here.
PDF is available here.
Abstract
We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS....
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PMID: 21031304
PDF is available here.
PDF is available here.
Abstract
We describe a methodology to heterologously express, purify, and conduct in vitro characterization of several metalloforms of the AHL lactonase from Bacillus thuringiensis (AiiA). These procedures should be applicable to similar enzymes and will facilitate the production of more useful quorum-quench...
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PMID: 21031319
PDF is available here.
PDF is available here.
Abstract
We describe the basic principles of growing hESCs in a chemically defined medium (CDM) devoid of serum and feeders....
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PMID: 21042984
PDF is available here.
PDF is available here.
Abstract
We present an improved osteogenic differentiation protocol based on the hanging drop method and a time-optimized use of 1α,25-(OH)(2) vitamin D(3), all-trans retinoic acid, and bone morphogenetic protein 2 (BMP-2) with an end point efficiency increased up to 90% and a protocol for chondrogenic diff...
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PMID: 21042995
PDF is available here.
PDF is available here.
Abstract
Proliferation activities of osteoblasts were significantly higher in the uncoated titanium fiber web. Osteoblasts in the uncoated titanium fiber web showed a typical expression pattern, but those in the HA-coated titanium fiber web showed rapid osteocalcin expression and calcification at an early st...
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PMID: 21483876
PDF is available here.
PDF is available here.
Abstract
Amounts of DNA and RNA was increased (from 20 to 50%) in the presence of salicylic acid in cells of Polyscias filicifolia tissue culture grown in Murachige-Skoog modified medium. Treatment of the tissue culture with salicylic acid resulted in a significant increase of intracellular protein and decrea...
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PMID: 21950124
PDF is available here.
PDF is available here.
Abstract
Four cultures of fungi of the genus Penicillium belonging to Furcatum Pitt subgenus, such as P. citrinum Thom, 1910; P. corylophilum Dierckx, 1901; P. fellutanum Biourge, 1923; and P. waksmanii Zaleski, 1927, produced the ergot alkaloids, namely, agroclavine-I, and epoxyagroclavine-I; their N-N-dimer...
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PMID: 21950123
PDF is available here.
PDF is available here.
Abstract
9alpha-Hydroxy derivatives were prepared from 11 steroids ofandrostane and pregnane series using Rhodococcus erythropolis VKPM Ac-1740 culture with 0.5-20 g/l substrate concentration in the reaction mixture. 9alpha-Monohydroxylation proceeded regardless of the substituent structure at C17. However, t...
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PMID: 21950117
PDF is available here.
PDF is available here.
Abstract
Peculiarities of synthesis of surface-active substances (SAS) are studied at periodical cultivation of Rhodococcus erythropolis EK-1 in the AK-210 fermenter on medium containing n-hexadecane. Maximum indicators of SAS synthesis (concentration of extra cellular SAS is 7.2 g/l; factor of emulsification...
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PMID: 21950118
PDF is available here.
PDF is available here.
Abstract
The selective Granada medium may be used concurrently with liquid Todd Hewitt Broth as a screening tool for prenatal group B streptococcal colonisation in pregnant women....
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PMID: 21712783
PDF is available here.
PDF is available here.
Abstract
Histoplasma capsulatum is the agent of histoplasmosis, a deep mycosis mainly afflicting immunocompromised patients. Rapid identification of the fungus isolated from clinical specimens allows timely administration of specific treatment.
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PMID: 21147250
PDF is available here.
PDF is available here.
Abstract
We studied 79 isolates, in which the most prevalent species using the system ID32C and PCR was C. albicans, followed by C. tropicalis, C. glabrata and C .krusei. PCR patterns obtained for the identification of clinical isolates were stable and consistent in the various independent studies and showed...
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PMID: 21167300
PDF is available here.
PDF is available here.
Abstract
Biocontrol Science (15)4 2010
Abstract
Our data reveal that the new method considerably shortens the time required for detection of yeasts and molds in food....
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PMID: 21212506
PDF is available here.
PDF is available here.
Biocontrol Science (15)4 2010
Abstract
Compact Dry X-SA (CD-XSA), a ready-to-use and self-diffusible dry medium sheet culture system for the detection and enumeration of Staphylococcus aureus, was evaluated. A total of 50 S. aureus strains, which were studied for the inclusivity study, grew as blue-colored colonies on the CD-XSA. When 11...
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PMID: 21212508
PDF is available here.
PDF is available here.
Bioresource Technology (101)23 2010
Abstract
The prospects of utilizing pretreated seawater for the culture of Arthrospira (Spirulina) platensis was evaluated under laboratory conditions with three seawater media and a control: (1) Zarrouk media (freshwater-control) (2) seawater media SW 1 (3) seawater media SW2 and (4) seawater media SW 3. Th...
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PMID: 20655201
PDF is available here.
PDF is available here.
Abstract
Brain research (1360) 2010
Abstract
We investigated the effect of ES on nerve growth factor (NGF)-induced neuronal differentiation, migration, neuritogenesis, and neurite extension. ES partially inhibited PC12 cell differentiation and cerebellar granule cell migration. In addition, neurite outgrowth was inhibited in a concentration-de...
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PMID: 20846515
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2010)11 2010
Abstract
We describe a method for culturing P. murrayi extracted from soil and sediment samples from the McMurdo (MCM) Dry Valleys of Antarctica. Nematodes are cultured on sand agar plates and incubated at various temperatures, and feed on bacteria growing on the agar media. They can be subcultured and store...
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PMID: 21041395
PDF is available here.
PDF is available here.
Abstract
To induce hairy roots of Gynostemm apentaphyllum by Agrobacterium rhizogenes strains.
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PMID: 21434425
PDF is available here.
PDF is available here.
Anti-Cancer Drugs (21)10 2010
Abstract
We have treated two neuroblastoma cell lines, SH-SY5Y and LA-N-1, with the spermine analogue N1, N11-Diethylnorspermine (DENSPM). SH-SY5Y was the most sensitive cell line, in which DENSPM treatment resulted in an inhibition of cell proliferation and an induction of cell death. The cell death induced...
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PMID: 20729713
PDF is available here.
PDF is available here.
Parasitology (137)13 2010
Abstract
We aimed to define a suitable method for cryopreserving viable Dientamoeba trophozoites. A modified BD medium, TYGM-9, Loeffler's slope medium, Robinson's medium, Medium 199, Trichosel and a Tritrichomonas fetus medium were compared, using cell counts, for their ability to support the growth of D. f...
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PMID: 20609278
PDF is available here.
PDF is available here.
Parasitology (137)13 2010
Abstract
Cryptosporidium parvum, belonging to the phylum Apicomplexa, is a major cause of waterborne gastroenteritis throughout the world. The sporozoites are thought to invade host enterocytes using an active process termed gliding motility. However, the biological and morphological changes within the sporo...
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PMID: 20800015
PDF is available here.
PDF is available here.
Abstract
Allelochemicals released from root exudates or decaying residues of plants play diversified roles in ecological interactions of plant-pathogen. The objective of this work was to evaluate the allelopathic effect of an externally supplied tannic acid on soil-borne in vitro Fusarium oxysporum f. sp. niv...
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PMID: 21506492
PDF is available here.
PDF is available here.
Biotechnology Letters (32)11 2010
Abstract
We isolated MDSCs from GFP-transgenic mice and transferred them to chondrogenic, neurogenic or myogenic media. Multilineage differentiation was examined by morphological observation, histological staining, immunocytochemical staining, real-time RT-PCR and Western blot. Both differentiated cells and...
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PMID: 20632072
PDF is available here.
PDF is available here.
Abstract
We compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Conco...
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PMID: 20801167
PDF is available here.
PDF is available here.
Abstract
We isolated several new LolCDE mutants that cause outer membrane localization of lipoproteins possessing LolCDE-avoidance signals. Mutations were found in all three subunits of LolCDE, including the cytoplasmic ATPase subunit LolD. However, the extent of outer membrane sorting of inner membrane-spec...
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PMID: 20888319
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)11 2010
Abstract
We screened actinomycetes capable of converting AS1387392 to AS1429716 and identified those strains capable of hydroxylation. Amycolatopsis azurea JCM 3275 was found to be a particularly efficient strain, capable of converting AS1387392 to AS1429716, with a yield of 44% after 9 h. This strain can...
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PMID: 20924384
PDF is available here.
PDF is available here.
Abstract
We show that, in many cell types, p53 confers increased survival in the face of chronic starvation. We implicate regulation of autophagy in this effect. In HCT116 human colorectal cancer cells exposed to prolonged nutrient deprivation, the endogenous wt p53 posttranscriptionally down-regulates LC3,...
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PMID: 20937856
PDF is available here.
PDF is available here.
Abstract
We investigated the potential of human fetal hepatocytes (hFH) in a four-compartment hollow fiber-based three-dimensional (3D) perfusion culture system. hFH were isolated from 17- to 19-gestational-week livers and cultured in the 3D perfusion bioreactors for 14 days. Metabolism activity, hepatocyte-...
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PMID: 19883207
PDF is available here.
PDF is available here.
Abstract
We used the combined imaging modality of multiphoton autofluorescence and second-harmonic generation microscopy to investigate the chondrogenic process of human mesenchymal stem cells cultured in chitosan scaffold. Isolated human mesenchymal stem cells seeded onto chitosan scaffold were induced to u...
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PMID: 19908965
PDF is available here.
PDF is available here.