Abstract
We provide a sample preparation method for quantitative proteomics of cellular fungi. Two different protein extraction methods were compared with focus on reproducibility, minimized proteolytic degradation and protein losses during the sample preparation. In the first preparation the cells were lyse...
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PMID: 18369902
PDF is available here.
Abstract
Allergens are molecules with the capacity to elicit IgE responses in humans. When stimulated with allergens, most allergic patients respond with production of IgE specific for several proteins/allergens in the source material. The standardization of allergen extracts is essential in order to control...
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PMID: 18612605
PDF is available here.
Abstract
This study was conducted to assess inhalation exposure to dust, endotoxin, and microorganisms (including viable bacteria, Gram-negative bacteria [GNB], and fungi) during waste collection and sorting; to identify factors affecting this exposure; and to estimate the gastrointestinal exposure to microor...
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PMID: 21516941
PDF is available here.
Abstract
To develop a multiplex PCR method used to fast diagnose pathogenic fungi of fungal rhinosinusitis.
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PMID: 21761713
PDF is available here.
Abstract
We compared children living on farms with those in a reference group with respect to the prevalence of asthma and atopy and to the diversity of microbial exposure. In one study--PARSIFAL (Prevention of Allergy-Risk Factors for Sensitization in Children Related to Farming and Anthroposophic Lifestyle...
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PMID: 21345099
PDF is available here.
Abstract
This review surveys the biological activities and the iterative and recursive biosynthetic mechanisms of fungal cyclooligomer depsipeptides, and their structural diversification by various combinatorial biosynthetic methods.
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PMID: 20959929
PDF is available here.
Abstract
We test the hypotheses that inoculation of soil from late secondary succession grasslands in arable receptor soil enhances performance of late successional plants, especially after top soil removal but pending on the added dose. To test this we grew mixtures of late successional plants in arable top...
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PMID: 21760929
PDF is available here.
Abstract
We developed a method coupled with a program named "PHYLORPH" (PHYLogenetic markers for ORPHans). The method screens fungal genomic databases (107 fungal genomes fully sequenced) for single copy genes that might be easily transferable and well suited for studies at low taxonomic levels (for example,...
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PMID: 21533204
PDF is available here.
Abstract
The storage of the different microorganisms over long periods is necessary to ensure reproducible results and continuity in research and in biomedical processes and also for commercial purposes. Effective storage means that a microorganism is maintained in a viable state free of contamination or gen...
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PMID: 20949399
PDF is available here.
Abstract
I argue that studying these specialized systems will help to reveal the basis for fungal diversity and provide general principles for co-evolution of organelles and multicellular complexity.
Copyright © 2010 Elsevier Ltd. All rights reserved....
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PMID: 20888233
PDF is available here.
Abstract
These results suggest that Cu/ZnSOD may play a larger role than MnSOD as a superoxide anion scavenger against oxidative stress in H. cunea.
Copyright © 2010 Elsevier Inc. All rights reserved....
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PMID: 20728562
PDF is available here.
Abstract
Our data reveal that the new method considerably shortens the time required for detection of yeasts and molds in food....
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PMID: 21212506
PDF is available here.
Abstract
To determine the etiologic diagnosis of infectious corneal ulcers at Tilganga Institute of Ophthalmology, a tertiary teaching hospital in Kathmandu Nepal, from 2006-2009.
This study involved a review of all microbiology records at Tilganga Institute of Ophthalmology from August 2006 through July 200...
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PMID: 20847686
PDF is available here.
Abstract
In the December 2009 issue of this journal, Orlandi et al presented a study in which peripheral blood mononuclear cells (PBMCs) from chronic rhinosinusitis (CRS) patients (5 from Texas, 5 from Utah) and seven nonhealthy controls were stimulated with fungal extracts. Despite the small numbers, they c...
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PMID: 20974326
PDF is available here.
Abstract
The hypothesis that fungus causes most, if not all, cases of chronic rhinosinusitis (CRS) has been debated for over a decade. Many opinions and interpretations have been rendered, but it is the objective data that speaks the loudest. The debate simply boils down to a core tenet of the scientific met...
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PMID: 20974327
PDF is available here.
Abstract
(-)-Sclerotiorin, isolated and purified from the fermented broth of an unidentified marine fungus (98F134), inhibited the maturation of starfish oocytes induced by 1-methyl adenine with an IC50 value of 0.50 microM. (-)-Sclerotiorin also showed antifungal activity against Saccharomyces cerevisiae, A...
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PMID: 21213982
PDF is available here.
Abstract
Virtually all organisms exposed to light are capable of sensing this environmental signal. In recent years the photoreceptors that mediate the ability of fungi to "see" have been identified in diverse species, and increasingly characterized. The small sizes of fungal genomes and ease in genetic and...
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PMID: 20451644
PDF is available here.
Abstract
The goal of this comparative study was to investigate biofilm forming microorganisms living in washing machines (WMs). Biofilms were sampled from 11 washing machines from four countries and three continents. Among the 94 isolated strains, 30% were potential human pathogens. Representative strains we...
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PMID: 20954022
PDF is available here.
Abstract
We present a method using generalized linear mixed models in a multilevel modeling framework to analyze patterns of variances and correlations in binomially distributed prevalence data. We then apply it to a multi-lake, multiyear data set involving two Daphnia host species and nine microparasite spe...
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PMID: 21141193
PDF is available here.
Abstract
We have adapted a clone-FISH approach known from prokaryotes to optimize the hybridization conditions of a designed oligonucleotidic probe specific to Chytridiales (i.e. the largest group of the true-fungal division of Chytridiomycota), before application to natural samples using the CARD-FISH appro...
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PMID: 20849888
PDF is available here.
Abstract
V-Xtractor (http://www.cmde.science.ubc.ca/mohn/software.html) uses Hidden Markov Models to locate, verify, and extract defined hypervariable sequence segments (V1-V9) from bacterial, archaeal, and fungal small-subunit rRNA sequences. With a detection efficiency of 99.6% and low susceptibility to fa...
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PMID: 20804791
PDF is available here.
Jay K Varma,
Kimberly D McCarthy,
Theerawit Tasaneeyapan,
Patama Monkongdee,
Michael E Kimerling,
Eng Buntheoun,
Delphine Sculier,
Chantary Keo,
Praphan Phanuphak,
Nipat Teeratakulpisarn,
Nibondh Udomsantisuk,
Nguyen H Dung,
Nguyen T N Lan,
Nguyen T B Yen and
Kevin P Cain
Abstract
We performed a single Myco/F Lytic blood culture. Fifty-eight (2.9%) had a clinically significant BSI (i.e., a blood culture positive for an organism known to be a pathogen). Mycobacterium tuberculosis accounted for 31 (54%) of all BSIs, followed by fungi (13 [22%]) and bacteria (9 [16%]). Of patien...
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PMID: 20875282
PDF is available here.
Abstract
To determine the fungal populations in seven ochratoxin A contaminated root herbs in Jiangxi province, and investigate the mycoflora associated with mycotoxin contamination in the root herbs.
Single spore isolation was used to obtain the strains from root herb's surface. Fungi were identified accord...
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PMID: 21246810
PDF is available here.
Abstract
We performed in silico analyses of nitrogen transporters and related assimilation genes in CCMP1545 and RCC299 and compared these with other green lineage organisms as well as Chromalveolata, fungi, bacteria, and archaea. Phylogenetic reconstructions of ammonium transporter (AMT) genes revealed dive...
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PMID: 20457585
PDF is available here.
Abstract
Communities of archaea, bacteria, and fungi were examined in forest soils located in the Oregon Coast Range and the inland Cascade Mountains. Soils from replicated plots of Douglas-fir (Pseudotsuga menziesii) and red alder (Alnus rubra) were characterized using fungal ITS (internal transcribed space...
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PMID: 20449582
PDF is available here.
Abstract
Our previous in situ study showed that bryophyte-microorganism complexes were affected by particulate atmospheric pollution. Here, the effect of urban particulate wet deposits on microbial communities living in bryophytes was studied under controlled conditions. An urban particulate solution was pre...
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PMID: 20674976
PDF is available here.
Abstract
New four Schiff bases are prepared by condensation of 2-amino-pyridin-3-ol with 3, 4-dihydroxy-benzaldehyde (I), 2-hydroxybenzaldehyde (II), 5-bromo-2-hydroxybenzaldehyde (III), and 4-dimethylaminobenzaldehyde (IV). The structures of these compounds are characterized based on elemental analyses (C....
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PMID: 20638329
PDF is available here.
Abstract
A series of novel 1H-dibenzo[a,c]carbazole derivatives were synthesized in good yield through reaction of methyl 7-oxo-dehydroabietate with a variety of substituted phenylhydrazines. The structures of the newly synthesized compounds were confirmed by IR, (1)H NMR, MS spectral studies and elemental a...
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PMID: 20702006
PDF is available here.
Abstract
A series of 1-(1H-1,2,4-triazol-1-yl)-2-(2,4-difluorophenyl)-3-substituted-2-propanols (1a-v, 2a-w), which are analogues of fluconazole, have been designed and synthesized as the potential antifungal agents by the click reaction. Click reaction approach toward the synthesis of two sets of novel 1,2,...
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PMID: 20675019
PDF is available here.
Abstract
A series of novel sulfanilamide-derived 1,2,3-triazole compounds were synthesized in excellent yields via 1,3-dipolar cycloaddition and confirmed by MS, IR and NMR spectra as well as elemental analyses. All the compounds were screened in vitro for their antibacterial and antifungal activities. Preli...
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PMID: 20708826
PDF is available here.
Abstract
The triazole analogs were obtained via. multistep synthesis sequence beginning with ethyl nicotinoate 3 which on treatment with hydrazine hydrate yields nicotinoyl hydrazide 4. Intermolecular cyclisation of 4 with 4-methylbenzoic acid in presence of phosphorous oxy chloride affords 2-(3-pyridyl)-5-(...
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PMID: 20630629
PDF is available here.
Abstract
A fermented soybean paste known as doenjang is a traditional fermented food that is widely consumed in Korea. The quality of doenjang varies considerably by its basic ingredients, species of microflora, and fermentation process. The classification of predefined metabolites (e.g. amino acids, organic...
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PMID: 20572059
PDF is available here.
Abstract
Locoweeds including the toxic species of Astragalus spp and Oxytropis spp. are widely distributed in the western region of China and result in a chronic neurological disease known as locoism in animals. To determine the presence of swainsonine-producing fungal endophyte of major locoweed species in...
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PMID: 20380845
PDF is available here.
Abstract
We show how these two factors can be separated by multivariate logistic regression, with the regression part accounting for species-specific habitat requirements, and a correlation matrix for the positive or negative residual associations. We parameterize the model using Bayesian inference with data...
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PMID: 20957941
PDF is available here.
Abstract
We examined whether interactions between tree seedlings and their associated arbuscular mycorrhizal fungi (AMF) lead to plant-soil feedback. Specifically, do tropical seedlings modify their own AMF communities in a manner that either favors or inhibits the next cohort of conspecific seedlings (i.e.,...
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PMID: 20957954
PDF is available here.
Abstract
We excluded foliar fungal pathogens from perennial grassland by using fungicide to determine the effect of natural levels of disease on an otherwise undisturbed plant community. Importantly, we excluded foliar fungal pathogens along with rabbits, insects, and mollusks in a full factorial design, whi...
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PMID: 20957952
PDF is available here.
Abstract
Symbiosis has long been associated with saltational evolutionary change in contradistinction to gradual Darwinian evolution based on gene mutations and recombination between individuals of a species, as well as with super-organismal views of the individual in contrast to the classical one-genome: on...
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PMID: 20535601
PDF is available here.
Abstract
Our team is investigating a diverse library of filamentous fungi for new anticancer leads. To avoid reisolating MTs through bioactivity-directed fractionation studies, a protocol for their facile dereplication was developed. The method uses readily available photodiode array detectors to identify on...
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PMID: 20648023
PDF is available here.
André P Liesen,
Thiago M de Aquino,
Cristiane S Carvalho,
Vânia T Lima,
Janete M de Araújo,
José G de Lima,
Antônio R de Faria,
Edésio J T de Melo,
Antonio J Alves,
Elias W Alves,
Anselmo Q Alves and
Alexandre J S Góes
Abstract
We reported the synthesis and evaluation of anti-Toxoplasma gondii and antimicrobial activities in vitro of three new compound series obtained from ethyl(5-methyl-1-H-imidazole-4-carboxylate): acylthiosemicarbazide analogues 3a-d, 4-thiazolidinone analogues 4a-d and 1,3,4-thiadiazole analogues 5a-d....
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PMID: 20541294
PDF is available here.
Abstract
We found that the THF ring is crucial for the remarkably selective activity of aureothin analogues against certain pathogenic fungi....
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PMID: 20662518
PDF is available here.
Abstract
To investigate the influence of N- or C-terminal regions of pleurocidin (Ple) peptide on the antifungal activity, four analogues partially truncated in the N- or C-terminal regions were designed and synthesized. Circular dichroism (CD) spectroscopy demonstrated that all the analogues maintained an a...
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PMID: 20798581
PDF is available here.
Abstract
We report the generation of the first monoclonal antibody that specifically binds to the polysaccharide chitosan. Mice were immunized with a mixture of chitosans, and hybridoma clones were screened for specific binders resulting in the isolation of a single clone secreting a chitosan-specific IgM, m...
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PMID: 20798578
PDF is available here.
Abstract
Glyceollins, one of the inducible phytoalexins produced by plants, were induced in a number of varieties of Korean soybean through fungal infection. Of the tested soybean varieties, Tae-Kwang, though not the most productive, was found to be currently the most suitable for the induction of glyceollin...
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PMID: 20798587
PDF is available here.
Abstract
Pathogens were isolated from 44% of all patients. MM patients more frequently had multiple pathogens in blood cultures (38% versus 25%). Transplantation related mortality was similar between the groups. CONCLUSION: The frequency of isolated pathogens, positive blood cultures, and the diversity of pa...
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PMID: 20670481
PDF is available here.
Abstract
I highlight the advances made by my team in this area during the past 3 years.
Copyright (c) 2010 John Wiley & Sons, Ltd....
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PMID: 20641013
PDF is available here.
Abstract
In all organisms, fatty acid synthesis is achieved in variations of a common cyclic reaction pathway by stepwise, iterative elongation of precursors with two-carbon extender units. In bacteria, all individual reaction steps are carried out by monofunctional dissociated enzymes, whereas in eukaryotes...
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PMID: 20731893
PDF is available here.
Abstract
Chitosan was isolated and purified from the mycelia of Rhizomucor miehei and Mucor racemosus. To characterize the extracted materials, their FTIR spectra were compared with that of shrimp chitosan. Final degree of deacetylation which determined by (1)H NMR was obtained as 98.6% for chitosan from R....
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PMID: 20471417
PDF is available here.
Abstract
A defective mucociliary clearance facilitates colonization with bacteria and fungal spores in cystic fibrosis patients. Yeasts and molds are cultured from the cystic fibrosis respiratory tract and often their clinical relevance is unknown. Candida spp. are the most commonly isolated yeasts, whereas...
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PMID: 20695750
PDF is available here.
Abstract
Wheat straw was submitted to a pre-treatment by the basidiomycetous fungi Euc-1 and Irpex lacteus, aiming to improve the accessibility of cellulose towards enzymatic hydrolysis via previous selective bio-delignification. This allowed the increase of substrate saccharification nearly four and three t...
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PMID: 20307975
PDF is available here.
Abstract
We discuss these considerations toward developing a framework for the Optimization and Risk Analysis of Complex Living Entities (ORACLE) - a computational method that integrates available information into a mathematical structure to calculate control coefficients.
Copyright 2010 Elsevier Ltd. All r...
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PMID: 20646768
PDF is available here.
Abstract
The contamination of soil with aromatic compounds is of particular environmental concern as they exhibit carcinogenic and mutagenic properties. One of the methods of their removal from soil is bioaugmentation, defined as a technique for improvement of the degradative capacity of contaminated areas b...
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PMID: 19735995
PDF is available here.
Abstract
Although a number of studies have indicated that microbes are key players in nutrient cycling, limitations on how to accurately assess their diversity have constrained further knowledge on the role of microbial diversity in organic matter decomposition in streams. Microbial diversity on leaf litter...
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PMID: 19720514
PDF is available here.
Abstract
We discovered that the microtubule-mediated transport of mRNAs is essential for the fast polar growth of infectious filaments in the corn pathogen Ustilago maydis. Combining in vivo UV cross-linking and RNA live imaging revealed that the RNA-binding protein Rrm4, which constitutes an integral part o...
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PMID: 20472693
PDF is available here.