Abstract
Silica-based columns are largely used in RNA purification, allowing fast extractions and good yields of high quality nucleic acid, but their major limitation is the high cost. The reuse of such columns, although desirable, is not recommended because of residual amounts of material from the previous...
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PMID: 21117284
PDF is available here.
Abstract
I and MFX II, from the thermophilic fungus Malbranchea flava MTCC 4889 with molecular masses of 25.2 and 30kDa and pIs of 4.5 and 3.7, respectively were purified to homogeneity. The xylanases were optimally active at pH 9.0 and at 60 degrees C, exhibited a half-life of 4h at 60 degrees C, and showed...
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PMID: 20630749
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Abstract
Streptomyces sp. 7b showed highest xylanase activity among 41 bacterial isolates screened under submerged fermentation. The organism grew over broad pH (5-11) and temperatures range (25-55 degrees C) and displayed maximum xylanase production on wheat bran (1230 U/g) under solid-state fermentation. X...
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PMID: 20419509
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Abstract
The purpose of this study was to analyze the ions released from a surface pre-reacted glass ionomer (S-PRG) filler in distilled water or lactic acid solution. S-PRG filler was mixed with either solution at 1000:1, 100:1, 10:1, and 1:1 ratios by weight. By means of inductively coupled plasma atomic e...
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PMID: 20610878
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Abstract
The electrochemical behavior of triflusal (TRF) and aspirin (ASA), before and after hydrolysis in water and in alkaline medium using two different electrode surfaces, glassy carbon and boron doped diamond, was study by differential pulse voltammetry over a wide pH range. The hydrolysis products are...
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PMID: 20402644
PDF is available here.
Abstract
Chromatographic determination of glutathione disulfide (GSSG) without any preliminary reduction has been presented using GSSG derivatization by p-hydroxymercuribenzoate (pHMB) in strong alkaline medium followed by the determination of GS-pHMB complex by reversed phase chromatography coupled to chemi...
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PMID: 20602975
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Abstract
These results demonstrate that the increase of biosorption capacity for Cu(2+) by ethanol and caustic-baker's yeast was attributed to the increase and exposure of carboxyl and amino groups on the surface of biomass sample.
Copyright 2010 Elsevier B.V. All rights reserved....
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PMID: 20226588
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Abstract
Alkaline graphite intercalation compounds were prepared by flake graphite, potassium dichromate, concentrated sulfur acid and sodium hydroxide under ultrasound irradiation and characterized by scanning electron microscopy and X-ray diffraction. The influences of solution alkalinity, bath temperature...
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PMID: 20202883
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Abstract
The dewaxed cell walls of Populus gansuensis were delignified with NaClO(2) and then sequentially extracted with 0.25, 0.5, and 1.0 M KOH under a solid to liquid ratio of 1: 25 (g mL(-1)) at 25 degrees C for 10 h. The successive treatments together resulted in the dissolution of 83.7% of original he...
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PMID: 20302372
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Abstract
We have performed substitution of strongly electron-withdrawing perfluoromethyl (CF(3)) group(s) as heme side chain(s) of Mb to obtain large alterations of the heme electronic structure in order to elucidate the relationship between the O(2) affinity of Mb and the electronic properties of heme perip...
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PMID: 20392104
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Abstract
At final examination, the dexamethasone, bevacizumab 5.0 mg/day and dexamethasone + bevacizumab groups showed a significant lowering in corneal opacity score as compared with control (P = 0.024, P = 0.006 and P = 0.013, respectively). Also, a significant reduction on new vessels size score was obser...
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PMID: 21077280
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Abstract
Xylanase A from Paenibacillus barcinonensis, a new species isolated from a rice field, has been cloned and expressed in Escherichia coli. Purified recombinant xylanase showed high activity on xylans from hardwoods and cereals, and exhibited K(m) and V(max) of 2.93 mg/mL and 50.67 U/mg on birchwood x...
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PMID: 20218604
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Abstract
Several oligo(p-phenylene-vinylene) oligomers capped with a guanosine or a guanine moiety have been prepared via a palladium-catalyzed cross-coupling reaction. Their self-assembly, in both the absence and presence of alkaline salts, has been studied by means of different techniques in solution (NMR,...
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PMID: 20039610
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Abstract
We review recent evidence from both the molecular and clinical realms to underline the importance of [H+] in the development and treatment of uric acid nephrolithiasis....
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PMID: 20425021
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Abstract
We identified osa-MIR396c, which shows dramatic transcript change under salt and alkali stress conditions in Oryza sativa. We designed an experiment to detect miRNA-target interaction and demonstrated that several transcription factors related to growth, development, and stress tolerance are targete...
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PMID: 20135324
PDF is available here.
Abstract
We present experimental results for a high-power diode pumped hydrocarbon-free rubidium laser with a scalable architecture. The laser consists of a liquid cooled, copper waveguide which serves to both guide the pump light and to provide a thermally conductive surface near the gain volume to remove h...
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PMID: 20173972
PDF is available here.
Abstract
Gene therapy with the recombinant retroviral vector-hosted mEndo and msFlk-1 gene effectively inhibited corneal NV induced by alkaline burn. The combination of multiple anti-angiogenic genes might be necessary for effective therapy of corneal NV, although each of these pathways makes a potential tar...
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PMID: 20208988
PDF is available here.
Abstract
Subconjunctival injection of Ad-Vasohibin-1 significantly reduces corneal neovascularization in alkali-treated mouse corneas. This effect of anti-neovascularization may be related to the downregulation of Vegfr2 expression....
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PMID: 20680097
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Abstract
We examined the possibility of facilitated excretion of uric acid by change in urine pH by managing food materials.
Within the framework of the Japanese government's health promotion program, we made recipes which consist of protein-rich and less vegetable-fruit food materials for H+-load (acid diet...
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PMID: 20955624
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Abstract
Bacterial surface layers, such as extracellular polymeric substances (EPS), are known to play an important role in metal sorption and biomineralization; however, there have been very few studies investigating how environmentally induced changes in EPS production affect the cell's surface chemistry a...
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PMID: 19915039
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Abstract
The background of this study comprises two clinical cases, where patients exposed to aerosols of an alkaline and surface active cleaning agent developed loss of enamel substance on their teeth, further resulting in loss of teeth and partially destroyed soft tissues. The alkaline cleaning agent consi...
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PMID: 21121412
PDF is available here.
Abstract
Cystinuria is an autosomal recessive disorder characterized by impairment of the proximal renal tubules which are unable to reabsorb cystine and dibasic amino acids, leading to the formation of recurrent kidney stones. According to the most recent genetic knowledge, there are two types of cystinuria...
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PMID: 20191458
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Abstract
Besides low mortality and morbidity rates in cardiac surgery, the associated cognitive dysfunction is the focus of interest. One possible reason is microembolisation.
The authors analysed the crystallogenesis in the calcium-containing prime, inspired by their observat...
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PMID: 20106942
PDF is available here.
Abstract
Besides low mortality and morbidity rates in cardiac surgery, the associated cognitive dysfunction is the focus of interest. One possible reason is microembolisation.
The authors analysed the crystallogenesis in the calcium-containing prime, inspired by their observat...
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PMID: 20106942
PDF is available here.
Abstract
Besides low mortality and morbidity rates in cardiac surgery, the associated cognitive dysfunction is the focus of interest. One possible reason is microembolisation.
The authors analysed the crystallogenesis in the calcium-containing prime, inspired by their observat...
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PMID: 20106942
PDF is available here.
Abstract
Besides low mortality and morbidity rates in cardiac surgery, the associated cognitive dysfunction is the focus of interest. One possible reason is microembolisation.
The authors analysed the crystallogenesis in the calcium-containing prime, inspired by their observat...
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PMID: 20106942
PDF is available here.
Abstract
OBJECTIVE: To study the chemical structures of macromolecular complexes formed by aconitine and glycyrrhizic acid, aconitine and rhein, berberine and rhein, ephedrine and glycyrrhizic acid in decoctions. METHOD: The binding energy of reactants and products was determined by X-ray spectrography, the...
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PMID: 20222423
PDF is available here.
Abstract
Chemical eye injury is considered an emergency that needs immediate nursing intervention because prognoses depend, not only on severity of injury, but also on the promptness and effectiveness of eye irrigation. Many nurse practitioners working in emergency care settings have limited knowledge of che...
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PMID: 20043429
PDF is available here.
Abstract
By functional complementation of Escherichia coli mutants defective in potassium (K(+)) uptake, two genes that are required for K(+) uptake in halo-alkaliphilic Alkalimonas amylolytica strain N10 were cloned. These two genes, Aa-trkA (1337 bp) and Aa-trkH (1452 bp), were adjacent on the A. amylolyti...
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PMID: 19911131
PDF is available here.
Abstract
OBJECTIVE: To study the vasodilation effects of the Total alkali Sophora alopecuroids L (TASa) on rabbit thoracic aortic rings in vitro and the possible mechanisms. METHOD: Rabbit aortic rings were isolated and precontracted with noradrenaline (NA) and then were divided into six groups including con...
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PMID: 20030093
PDF is available here.
Abstract
OBJECTIVE: To determine the change pattern of alkaline and acid components of Dahuang and Huangbai amoung different combinations in Dahuang Xiaoshi decotion. METHOD: The contents of anthraquinones and berberine were determined by HPLC in samples of Dahuang extracts, Huangbai extracts, Dahuang and Hu...
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PMID: 20030076
PDF is available here.
Abstract
OBJECTIVE: To determine the change pattern of alkaline and acid components of Dahuang and Fuzi in different combinations. METHOD: The contents of anthraquinones and aconite alkaloids were determined by HPLC in samples of Dahuang extracts, Fuzi extracts, Dahuang and Fuzi pair medicines extracts, and...
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PMID: 19943477
PDF is available here.
Abstract
We analyze here, by noninvasive electrophysiology, local and systemic plant responses in the interaction of barley (Hordeum vulgare L.) with the root-colonizing basidiomycete Piriformospora indica. In the short term (seconds, minutes), a constant flow of P. indica chlamydospores along primary roots...
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PMID: 19656052
PDF is available here.
Abstract
We have derived a conceptual model according to which sulfate is generated upon reaction of weathering-derived alkalinity with gypsum to form calcite, which is abundant in these areas. The model proposes that part of the alkalinity generated during weathering is stared as calcite in the landscape, w...
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PMID: 19764215
PDF is available here.
Abstract
Structural unfolding of soy protein isolate (SPI) as induced by holding (0, 0.5, 1, 2, and 4 h) in acidic (pH 1.5-3.5) and alkaline (pH 10.0-12.0) pH solutions, followed by refolding (1 h) at pH 7.0, was analyzed. Changes in emulsifying properties of treated SPI were then examined. The pH-shifting t...
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PMID: 19601630
PDF is available here.
Abstract
Sand-packed horizontal flow columns (5 cm i.d. x 65 cm)l were used in laboratory experiments to simulate in situ electrolytic and alkaline hybrid treatment zone for aqueous phase decomposition of RDX. An upgradient cathode and downgradient anode, spaced 35 cm apart were used to create alkaline reduc...
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PMID: 19746729
PDF is available here.
Abstract
We analyse a novel squeezing and entangling mechanism which is due to correlated Stokes and anti-Stokes photon forward scattering in a multi-level atom vapour. We develop a full quantum model for an alkali atomic vapour including quantized collective atomic states which predicts high degree of squee...
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PMID: 19654852
PDF is available here.
Abstract
OBJECTIVE: To determine the change pattern of alkaline and acid components of Fuzi and gancao amoung different combinations in Sini Decotion. METHOD: The contents of aconite alkaloids and glycyrrhizic acid were determined by HPLC in samples of Fuzi extracts, gancao extracts, Fuzi and gancao pair med...
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PMID: 19938542
PDF is available here.
Abstract
This study focuses on the surface modification of a near beta-type Ti-27 wt.% Nb alloy by alkali-heat treatment. The influence of alkali concentration, alkali-treated time and alkali-treated temperature on the microstructure and constitutional phases of the modified surface is invest...
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PMID: 19671955
PDF is available here.
Abstract
We evaluated DNA damage using alkaline comet assay applied on erythrocytes after in vivo exposure of Prochilodus lineatus to different concentrations of Cypermethrin (0.300, 0.150, 0.075 and 0.000 microg/L) as a probable chemical mutagen. The results revealed a significantly higher level of DNA dama...
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PMID: 19466374
PDF is available here.
Abstract
The changes observed in the in vitro organ culture model in response to a severe alkali burn are similar to those observed by other groups in clinical and in vivo studies. We believe that this or similar organ culture systems could be used to evaluate some aspects of severe alkali burns....
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PMID: 19421042
PDF is available here.
Abstract
CCR2-deficient mice exhibited reduced alkali-induced corneal neovascularization with reduced macrophage infiltration, whereas CX3CR1-deficient mice developed a more severe form of alkali-induced corneal neovascularization with reduced macrophage infiltration. Selective macrophage depletion by Cl2MDP...
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PMID: 19421039
PDF is available here.
Abstract
Corneal lymphangiogenesis provides an exit route for antigen-presenting cells to regional lymph nodes, inducing immune response. The purpose of this study was to examine the development of corneal lymphatic vessels in alkali-burned corneas.
Corneal lymphatic vessels w...
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PMID: 18811642
PDF is available here.
Abstract
The aim of this work was to optimize the enzymatic hydrolysis of the cellulose fraction of cashew apple bagasse (CAB) after diluted acid (CAB-H) and alkali pretreatment (CAB-OH), and to evaluate its fermentation to ethanol using Saccharomyces cerevisiae. Glucose conversion of 82 +/- 2 mg/g CAB-H and...
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PMID: 19031051
PDF is available here.
Abstract
We demonstrate that ferric reductase FRP1 expression is upregulated by alkaline pH and iron-limited conditions. Moreover, promoter deletion identified that the upregulated elements lie in -822 to 437 and 263 to -124 and the downregulated elements in -437 to -263 using a beta-galactosidase reporter s...
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PMID: 19076241
PDF is available here.
Abstract
U(VI) adsorption on aerosol-synthesized hematite particles ranging in size from 12 to 125 nm was studied to explore nanoscale size effects on uranium adsorption. Adsorption on 70 nm aqueous-synthesized particles was also investigated to examine the effect of the synthesis method on reactivity. Equil...
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PMID: 19350906
PDF is available here.
Abstract
We have developed and applied a robust nonparametric K-nearest neighbor (K-nn) bootstrap approach utilizing the United States Environmental Protection Agency's Information Collection Rule (ICR) data. In this technique, first an appropriate "feature vector" is formed from the best available explanato...
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PMID: 19350911
PDF is available here.
Abstract
To determine the relationship between soluble methylmercury and soil characteristics which was contaminated by mercury, several experiments were conducted. As a result, a good correlation was founding between the leached methylmercury level from soil and the EC (electronic conductivity) level of soi...
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PMID: 19050818
PDF is available here.
Abstract
Toxicological safety of new procedure for the neutralisation/purification of wastewater originated from zinc plating facility was investigated. Wastewater was treated with alkaline solid residue-by-product of zinc recovery from electric arc furnace dust. For determination of cytotoxic potential of u...
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PMID: 19132590
PDF is available here.
Abstract
We show here that this method is 92% efficient in sequestration of crop residue carbon while cellulosic ethanol production is only 32% and soil sequestration is about 14% efficient. Deep ocean sequestration can potentially capture 15% of the current global CO2 annual increase, returning that carbon...
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PMID: 19320149
PDF is available here.
Abstract
Column experiments combined with geochemical modeling, microscopic inspections, spectroscopic interrogations, and wet chemical extractions were used to study sediment-dependent Cr(VI) desorption, physical location, mineral association, and attenuation mechanism(s) in four freshly or naturally aged c...
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PMID: 19320160
PDF is available here.
Abstract
We discuss results from an investigation directed at understanding the role of salts doped as alkali chlorides in a model biological environment, arginine. The data show that addition of salt to the model system causes ion suppression of all the major mass spectral peaks attributed to arginine, with...
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PMID: 19125566
PDF is available here.
Abstract
We investigate the last stages of dehydration when the number of adsorbed water molecules corresponds, at maximum, to a monolayer. Weight losses are measured at several constant temperatures as a function of time. Application of Van't Hoff's law yields the dehydration enthalpy. Trends and data simil...
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PMID: 18973904
PDF is available here.
Abstract
Despite considerable progress in the field of metal nanoparticles synthesis, major challenges remain in many practical applications of nanoparticles which require their immobilization on solid substrates, presenting additional difficulty in separation and processing. Here, transparent nanoporous cel...
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PMID: 19053296
PDF is available here.
Abstract
We describe the analysis of these fractions with MudPIT, a proteomics methodology that avoids gel extraction of bands to facilitate identification of minor proteins and membrane proteins that do not resolve well on gels. Together these three approaches can significantly increase the output of proteo...
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PMID: 19153695
PDF is available here.
Abstract
We have successfully used over the years to detect UCP2 protein in various mouse and human specimens. These techniques include mitochondrial isolation and submitochondrial fractionation followed by Western blotting and UCP2 immunohistochemistry. We find that sample preparation is a key to success an...
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PMID: 19609759
PDF is available here.
Abstract
8-Hydroxydaidzein is a suicide substrate of mushroom tyrosinase with potent irreversible inhibitory activity. Despite its high potential in the cosmetics industry, it was found that 8-hydroxydaidzein was unstable in a formulated cream. In this technical note, the stability of 8-hydroxydaidzein in va...
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PMID: 19586603
PDF is available here.