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Biological Assay (2):
Recent article
Biological Assay
Abstract
We have modified the original apparatus for organ bioassay in order to minimize foaming and to increase analytical performance. By using this modified bioassay we investigated the vasodilatory potency of various endogenous RSNOs in the presence of physiologically relevant concentrations of albumin a...
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PMID: 21827780
PDF is available here.
PDF is available here.
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Abstract
The Quantitative Assay Database (QuAD), http://proteome.moffitt.org/QUAD/, facilitates widespread implementation of quantitative mass spectrometry in cancer biology and clinical research through sharing of methods and reagents for monitoring protein expression and modification....
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PMID: 21656910
PDF is available here.
PDF is available here.
Abstract
We present the results from the first two rounds of the program.
The program is open for laboratories using commercially available kits for Aβ, T-tau, or P-tau. CSF samples (aliquots of pooled CSF) are sent for analysis several times a year from the Clinical Neurochemistry Laborator...
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PMID: 21784349
PDF is available here.
PDF is available here.
Abstract
We found Fussel-15, a new member of the Ski/Sno family of TGF-β/BMP signaling repressors, to be expressed in early wound healing and constantly overexpressed in keloid-derived and scleroderma-derived fibroblasts. Comparing the results of three-dimensional free-floating and attached-released in vitr...
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PMID: 21641385
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We describe the protocols for measuring Rho-associated coiled-coil-containing kinase (ROCK) activity in vitro. A His-tagged, constitutively active form of the protein (lacking C-terminal inhibitory domains) is expressed in baculovirus. The protein is purified by a combination of metal affinity, ion...
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PMID: 19099256
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1813)5 2011
Abstract
We have developed a novel analytical method to quantify the amount of PI3P in endosomes by introducing a GST-2xFYVE protein probe into semi-intact cells. The GST-2xFYVE probe was targeted specifically to intracellular PI3P-containing endosomes, which retained their small punctate structure, and allo...
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PMID: 21277337
PDF is available here.
PDF is available here.
Veterinary Record (168)17 2011
Abstract
This study was undertaken to evaluate two monoclonal antibody-based sandwich ELISAs (sELISAs) for the detection of Clostridium botulinum neurotoxins (BoNTs) types C and D from culture-enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivabl...
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PMID: 21493446
PDF is available here.
PDF is available here.
Abstract
We studied the properties of perivascular cells from a novel source, the foetal human umbilical cord (HUC) collected from pre-term newborns. By immunohistochemistry and flow cytometry we show that pre-term/foetal HUCs contain more perivascular cells than their full-term counterparts (2.5%versus 0.15...
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PMID: 20219017
PDF is available here.
PDF is available here.
Abstract
We demonstrate the feasibility of this method in Drosophila melanogaster, utilizing the well-characterized HEG, I-SceI. In particular, we show that high rates of homing can be achieved within spermatogonia and in the female germline. We show that homed constructs continue to exhibit HEG activity in...
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PMID: 21368273
PDF is available here.
PDF is available here.
Abstract
We developed assays that quantify noxious heat avoidance in the nematode Caenorhabditis elegans and used them to investigate the genetic basis of this behavior. First, we screened mutants for 18 TRP channel genes (including all TRPV orthologs) and found only minor defects in heat avoidance in single...
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PMID: 21368276
PDF is available here.
PDF is available here.
Abstract
Biological control of sandflies using entomopathogenic fungi is a possible alternative to the expensive synthetic chemical control. It is potentially sustainable, less hazardous, and relatively inexpensive and merits further investigations. The objective of this study was to identify the most pathoge...
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PMID: 21406735
PDF is available here.
PDF is available here.
Bioanalysis (3)3 2011
Abstract
Louis-Philippe Morin is a senior instrument application specialist at Algorithme Pharma, a CRO located in Laval, Canada. He has been working in the bioanalysis industry for the past 10 years where he became a subject matter expert in analytical instrumentation, especially in the MS f...
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PMID: 21320048
PDF is available here.
PDF is available here.
Cancer Biology & Therapy (11)2 2011
Abstract
We determined the feasibility of using the ChemoFx DRM to test tumor samples for sensitivity to cetuximab. We exposed four non-small cell lung carcinoma (NSCLC) cell lines (H358, H520, HCC827, and H1666) to cetuximab and determined their sensitivity using the ChemoFx DRM and, in parallel, EGFR statu...
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PMID: 20980824
PDF is available here.
PDF is available here.
Abstract
We have developed and validated a multiplex SSP-PCR method for the genetic analysis of the KIR locus. Design and optimization of four multiplex groups targeting 14 genes and their alleles on the KIR locus has been completed. Each multiplex group contains PCR products that differ in size by a minimum...
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PMID: 21214526
PDF is available here.
PDF is available here.
Abstract
We evaluated 309 environmental chemicals, mostly food-use pesticides, from the ToxCast™ chemical library using a mouse ES cell platform. ES cells were cultured in the absence of pluripotency factors to promote spontaneous differentiation and in the presence of DMSO-solubilized chemicals at differe...
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PMID: 21666745
PDF is available here.
PDF is available here.
Abstract
Marine sponge Hymeniacidon sp. was collected from coastal waters of the East China Sea to isolate symbiotic microorganisms. The resulting sponge-associated actinomycete, Streptomyces carnosus strain AZS17, was cultivated in a 20 L volume of medium for production of bioactive secondar...
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PMID: 21556165
PDF is available here.
PDF is available here.
Abstract
Our knowledge of how this type of result is currently reflected in vivo is summarised, together with the 'naturally protective' properties of kiwifruit that involve modulating immune responses in a positive way. Finally, the ways in which these antioxidant and natural protective properties of kiwifr...
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PMID: 21506926
PDF is available here.
PDF is available here.
Abstract
We previously discovered a lead compound for strigolactone (SL) biosynthesis inhibitors, TIS13 (2,2-dimethyl-7-phenoxy-4-(1H-1,2,4-triazol-1-yl)heptan-3-ol). Here, we carried out a structure-activity relationship study of TIS13 to discover more potent and specific SL biosynthesis inhibitor because T...
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PMID: 21760901
PDF is available here.
PDF is available here.
Abstract
Our lab has developed a 3D co-culture method to grow interconnected networks of pericyte-invested capillaries, which can anastamose with host vasculature following implantation to restore blood flow to ischemic tissues. However, if the engineered vessels contain endothelial cells (ECs) that are misa...
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PMID: 21760956
PDF is available here.
PDF is available here.
Abstract
We describe an optimised labelling procedure using a short incubation time and low concentration of clinically used SPIO Endorem to successfully track murine DC migration in vivo using MRI in a mouse tumour model. First, intracellular labelling of bone marrow derived DCs was monitored in vitro using...
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PMID: 21637760
PDF is available here.
PDF is available here.
Abstract
We used cell lines genetically engineered for conditional asymmetric self-renewal, the defining DSC property. In gene microarray analyses, we identified 85 genes whose expression is tightly asymmetric self-renewal associated (ASRA). The ASRA gene signature prescribed DSCs to undergo asymmetric self-...
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PMID: 21818293
PDF is available here.
PDF is available here.
Abstract
We tested this using the glutamate-gated chloride channel (GluCl) gene family, which forms the ivermectin drug target and are candidate resistance genes. We expressed GluCl subunits from C. elegans and H. contortus in a highly resistant triple mutant C. elegans strain (DA1316) under the control of t...
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PMID: 21818319
PDF is available here.
PDF is available here.
Abstract
Harnessing the ability to precisely and reproducibly actuate fluids and manipulate bioparticles such as DNA, cells, and molecules at the microscale, microfluidics is a powerful tool that is currently revolutionizing chemical and biological analysis by replicating laboratory bench-top...
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PMID: 21072867
PDF is available here.
PDF is available here.
Abstract
The typical characteristics of cartilage and bone tissue are their unique extracellular matrices on which our body relies for structural support. In the respective tissue, the cells that create these matrices are the chondrocyte and the osteoblast. During in vitro differentiation from an embryonic o...
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PMID: 21042998
PDF is available here.
PDF is available here.
Abstract
We describe the basic methodology for producing 3D tissue-engineered skin and oral mucosa based on de-epidermised acellular human dermis, and we give examples of how these models can be used for a variety of applications....
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PMID: 21042970
PDF is available here.
PDF is available here.
Abstract
We report here a simple method to reproducibly differentiate human embryonic stem cells (hESCs) into MSCs that does not require the use of any feeder layers or exogenous cytokines. The cells obtained with this procedure have a normal karyotype, are morphologically similar to bone marrow MSCs, are co...
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PMID: 21042994
PDF is available here.
PDF is available here.
Abstract
Osteoblasts are the cells that contribute to the formation and function of bone tissue. Knowledge of their biology is important to understanding of the normal processes of bone repair, the development of diseases affecting bone tissue, and to the investigation of approaches to improve bone repair an...
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PMID: 21042979
PDF is available here.
PDF is available here.
Abstract
We describe here methods for the identification of osteoclasts formed in vitro....
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PMID: 21042999
PDF is available here.
PDF is available here.
Abstract
There are several types of bioreactors currently available for the culture of orthopaedic tissue engineered constructs. These vary from the simple to the complex in design and culture. Preparation of samples for bioreactors varies depending on the system being used. This chapter presents data and de...
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PMID: 21042966
PDF is available here.
PDF is available here.
Abstract
These results suggest that this method might have utility in the rational selection of active classes of compounds (and not just individual active compounds) for followup and validation....
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PMID: 21073183
PDF is available here.
PDF is available here.
Abstract
We report here a designer self-assembling peptide nanofiber scaffold developed specifically for nerve tissue engineering. We synthesized a peptide FGL-RADA containing FGL (EVYVVAENQQGKSKA), the motif of neural cell adhesion molecule (NCAM), and then attended to make a FGL nanofiber scaffold (FGL-NS)...
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PMID: 20878982
PDF is available here.
PDF is available here.
Abstract
An experimental validation of electropermeabilization inducing field distribution in tissue predicted by simulation for needle electrodes is described giving a direct visualization of the permeabilization of a 2D tissue.
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PMID: 20924523
PDF is available here.
PDF is available here.
Autoimmunity Reviews (10)2 2010
Abstract
Immunoglobulins in patients with Graves' disease (GD) that modulate the thyroid stimulating hormone receptor (TSH-R) do so via stimulating cAMP dependent signals (TSI), blocking TSH or inhibition of TSH-receptor activation (TBI) or inducing apoptotic signals. These functional immunog...
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PMID: 20807591
PDF is available here.
PDF is available here.
Nature Medicine (16)12 2010
Abstract
Abstract
New multivalent RGD-containing macromolecules were designed by exploiting two orthogonal chemoselective ligations. They were next applied to a competitive cell adhesion assay and used for the non invasive optical imaging of tumour in small animals.
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PMID: 20835451
PDF is available here.
PDF is available here.
Abstract
In order to minimize risks to human and environmental health, chemical safety assessment programs are being reinforced with toxicity tests more specifically designed for detecting endocrine disrupters. This includes the necessity to detect thyroid-disrupting chemicals, which may operate through a va...
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PMID: 20684730
PDF is available here.
PDF is available here.
Abstract
The DR-H4IIE.Luc bioassay is based on the ability of dioxin and dioxin-like contaminants to activate the AhR and its signal transduction pathway, a mechanism through which these contaminants elicit their toxic effects. The bioassay was used to examine the total dioxin-equivalent (TEQ) toxicity in go...
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PMID: 20863569
PDF is available here.
PDF is available here.
Abstract
We tried to identity the signaling mechanism and develop a system to assess pro-longevity status in cells and mice. We previously identified genes up-regulated in the liver of DF and CR mice by DNA microarray analysis. Motif analysis of the upstream sequences of those genes revealed four major conse...
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PMID: 20846506
PDF is available here.
PDF is available here.
Abstract
Post-translational modifications of histones play an important role in regulating chromatin dynamics and function. One of the modifications, methylation, occurs on both lysine and arginine residues, and methylation status defines the epigenetic program of a cell by determining chromatin structure an...
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PMID: 20889709
PDF is available here.
PDF is available here.
Annals of Medicine (42)7 2010
Abstract
The main objective of this 1.5-year prospective study was to evaluate the value of T-SPOT®.TB as compared to the tuberculin skin test (TST) for the first-line assessment of uveitis.
A total of 108 consecutive non-immunocompromised patients with acute or chronic uveitis, including 92/108 (85.2%) wit...
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PMID: 20868342
PDF is available here.
PDF is available here.
Abstract
A number of high molecular weight (HMW) lignin derivatives possessing varied chemical properties were screened for their biological effects in order to obtain more information on the possible structural features of HMW lignin-related effects. The studied compounds were both commercial and in-house e...
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PMID: 20494440
PDF is available here.
PDF is available here.
Abstract
Our results showed that the high polarity fraction of the hot water extract exhibited stimulatory effects on PBMC proliferation as shown in [methyl-(3)H]-thymidine incorporation assay. In an attempt to isolate the active components responsible for the activities, further partition with ethyl acetate...
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PMID: 20609432
PDF is available here.
PDF is available here.
Abstract
The response to a Parliamentary Question put to the then-Home Office Minister on 8 March 2006, was that "All protocols for the detection of toxins in shellfish intended for human consumption were assigned a substantial severity limit", and that "A total of 6,468 animals were used in the relevant pro...
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PMID: 21105757
PDF is available here.
PDF is available here.
Abstract
To clarify the relationship between pollen density and gametophytic competition in Pyrus pyrifolia, gametophytic performance, gibberellin metabolism, fruit set, and fruit quality were investigated by modifying P. pyrifolia pollen grain number and density with Lycopodium spores. Higher levels of poll...
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PMID: 20713466
PDF is available here.
PDF is available here.
Biochemical Journal (431)2 2010
Abstract
We demonstrate that the neurotoxicity of BoNT/D depends on the presence of gangliosides by employing phrenic nerve hemidiaphragm preparations derived from mice expressing the gangliosides GM3, GM2, GM1 and GD1a, or only GM3 [a description of our use of ganglioside nomenclature is given in Svennerhol...
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PMID: 20704566
PDF is available here.
PDF is available here.
Abstract
Six dominant strains were isolated from the livers of the puffer fish Fugu obscurus and were screened for their TTX production-ability. Electrospray ionization-mass spectrometry (ESI-MS) analyses revealed that strain B-1 produced TTX and related substances; mouse bioassay revealed that 23.9 mouse un...
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PMID: 20576513
PDF is available here.
PDF is available here.
Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances & Environmental Engineering (45)11 2010
Abstract
The aim of this study was to compare the sensitivity of two Corophidae: Corophium orientale a standardized species and Corophium insidiosum a species more available in the Ionian sea (Southern Italy), in order to evaluate the suitability and applicability of C. insidiosum to sediment toxicity test....
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PMID: 20694884
PDF is available here.
PDF is available here.
Abstract
To develop a new bioassay method using human lung epithelial cells (CCL-185) to assess activity of transforming growth factor beta (TGF-beta) in human tear fluid from normal subjects and patients with dry eye.
Two epithelial cell lines, mink lung cells (CCL-64) and human lung cells (CCL-185), were c...
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PMID: 20539212
PDF is available here.
PDF is available here.
Water Research (44)16 2010
Abstract
Quantitative assessment of multiple sources to short-term variations in recreational water quality, as indexed by faecal indicator organism (FIO) concentrations, is becoming increasingly important with adoption of modern water quality standards and catchment-based water quality management requiremen...
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PMID: 20630556
PDF is available here.
PDF is available here.
Abstract
We evaluated 22 different host and non-host plant protease inhibitors (PIs) for in vivo inhibition of Helicoverpa armigera gut pro- and proteinases, and their biological activity against the pod borer, H. armigera, the most important pest of agriculture and horticultural crops worldwide. In vitro ac...
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PMID: 20416317
PDF is available here.
PDF is available here.
Biophysical Journal (99)4 2010
Abstract
We hypothesize that tightly looping DNA is alone sufficient to repress transcription. To test this hypothesis, we have developed an assay to quantify transcription elongation by bacteriophage T7 RNAP on small, circular DNA templates approximately 100 bp in size. From these highly bent transcription...
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PMID: 20712997
PDF is available here.
PDF is available here.
Abstract
We describe the use of a microfluidic device to micropattern cells in a microchannel and investigated the behavior of these cells under a concentration gradient. The microfluidic device consisted of 3 parts: a branched channel for generating a stable concentration gradient, a main channel for cultur...
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PMID: 20547384
PDF is available here.
PDF is available here.
Cell Cycle (9)15 2010
Abstract
We describe here a novel, non-radioactive assay to measure p53-DNA binding which involves the sequential use of in vitro transcription/ translation (IVT), immunoprecipitation and real-time PCR. The method reliably enables the detection of sequence-specific DNA binding of full-length p53 at low conce...
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PMID: 20714218
PDF is available here.
PDF is available here.
Abstract
We have developed an efficient decellularization process for the isolation of extracellular matrix (ECM) from native cardiac tissue. The isolated ECM exhibited desirable mechanical properties in terms of elasticity, strength and durability-properties required from scaffolds used for cardiac tissue r...
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PMID: 19780649
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)8 2010
Abstract
We have investigated the effects of different concentrations of silk fibroin protein on three-dimensional (3D) scaffold pore microstructure. Four pore size ranges of silk fibroin scaffolds were made by the freeze drying technique, with the pore sizes ranging from 50 to 300 microm. The pore sizes of...
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PMID: 20188872
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)8 2010
Abstract
We cultured neonatal mouse cerebellum C17.2 cells on the PLLA fibers. These neural stem cells (NSCs) exhibited significantly different growth and differentiation depending upon fiber dimension and pattern. On aligned fibers cell viability and proliferation was best on 500 nm fibers, and reduced on s...
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PMID: 20193781
PDF is available here.
PDF is available here.
Abstract
We conclude that quantitative MRI may provide specific information on cell-matrix interaction and is a promising noninvasive approach to understand and monitor cell-seeded natural scaffold-based regeneration....
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PMID: 19772478
PDF is available here.
PDF is available here.
Abstract
We recently described a novel staphylococcal cassette chromosome mec (SCCmec) element, designated type VIII. We report here a multiplex PCR assay, capable of identifying SCCmec type VIII. This assay will facilitate identification of this new SCCmec type and provides a useful tool for clinical and ep...
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PMID: 20074636
PDF is available here.
PDF is available here.
Abstract
Clostridium chauvoei is the causative agent of blackleg in cattle and sheep. The clinical symptoms of this severe disease are very similar to that of malignant edema (Clostridium septicum), infections of other Clostridium species belonging to the gas edema complex, and anthrax (Bacillus anthracis)....
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PMID: 20362050
PDF is available here.
PDF is available here.
Abstract
We have developed an automated approach for the detection of these 6 common Sardinian mutations based on TaqMan technology. Ten DNA samples of WD patients carrying different combinations of the six most common Sardinian mutations and normal controls previously analysed were used in triplicate to set...
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PMID: 20138984
PDF is available here.
PDF is available here.