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Centrifugation (1):
Recent article
Centrifugation
Abstract
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Abstract
It is well known that accelerations during centrifuge training and during flight can provoke cardiac arrhythmias. Our study was designed to investigate both the similarities and differences between heart rhythm disturbances during flights and centrifuge tests.
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PMID: 21702309
PDF is available here.
PDF is available here.
Abstract
The spontaneous folding of two Neisseria outer membrane proteins, opacity-associated (Opa)(60) and Opa(50) into lipid vesicles was investigated by systematically varying bulk and membrane properties. Centrifugal fractionation coupled with sodium dodecyl sulfate polyacrylamide gel electrophoresis mobi...
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PMID: 21539780
PDF is available here.
PDF is available here.
Abstract
We propose a simple and highly sensitive method for phosphopeptide enrichment on NiO nanoparticles (NPs) from a trypsin predigested phosphoprotein complex solution in a microwave oven. Furthermore, this technique was combined with centrifugation on-particle ionization/enrichment of phosphopeptides a...
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PMID: 21533801
PDF is available here.
PDF is available here.
Abstract
In this chapter, a rapid method to isolate intact chloroplasts from spinach leaves is described. Intact chloroplasts are isolated using two short centrifugation steps and avoiding the use of percoll gradient. Intactness of chloroplast is evaluated by the inability of potassium ferricyanide to enter...
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PMID: 20960139
PDF is available here.
PDF is available here.
Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a multifunctional enzyme that catalyzes the fixation of CO2 and O2 in photosynthesis and photorespiration, respectively. As the rate-limiting step in photosynthesis, improving the catalytic properties of Rubisco has long been viewed as a v...
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PMID: 20960141
PDF is available here.
PDF is available here.
Abstract
Knowledge of factors affecting sample integrity is vital to make informed judgements on the validity of results. However, the information available for sample stability is incomplete, confusing and conflicting, particularly post-centrifugation. This study aims to investigate the effects of storage co...
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PMID: 21950208
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler....
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PMID: 20938554
PDF is available here.
PDF is available here.
Abstract
Separation of (90)Y from a parent solution of (90)Sr-(90)Y in 0.5 M nitric acid by two-stage liquid-liquid extraction has been investigated using centrifugal extractors. The extraction agent used was 0.25 M di-(2-ethylhexyl)phosphoric acid (D2EHPA) in n-dodecane. Back-extraction used 5 M HCl. Evapor...
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PMID: 20692176
PDF is available here.
PDF is available here.
Waste Management (30)11 2010
Abstract
In this research, a two-step process consisting of vacuum pyrolysis and vacuum centrifugal separation was employed to treat waste printed circuit boards (WPCBs). Firstly, WPCBs were pyrolysed under vacuum condition at 600 °C for 30 min in a lab-scale reactor. Then, the obtained pyrolysis residue wa...
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PMID: 20655190
PDF is available here.
PDF is available here.
Abstract
We compare the efficiency of several popular in-gel digestion methods, along with new technologies that may improve digestion efficiency, using a human epidermoid carcinoma cell lysate protein standard. The efficiency of each protocol was based on the average number of proteins identified and their...
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PMID: 20808644
PDF is available here.
PDF is available here.
Abstract
We have characterized a typical bioprocess and determined (a) how the time of harvest from a mammalian cell culture and, (b) through the use of an ultra scale-down mimic how the nature of the primary recovery stages, affect the distribution and make up of the PTMs observed on a recombinant IgG(4) mo...
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PMID: 20506289
PDF is available here.
PDF is available here.
Abstract
We describe a new approach based on centrifugation for obtaining highly concentrated yet porous collagen scaffolds. Water uptake, chondroitin sulfate retention, morphology, mechanical properties, and tissue-engineering potential of the concentrated scaffolds were investigated. Our results show that...
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PMID: 20694972
PDF is available here.
PDF is available here.
Abstract
Eukaryotic Cell (9)7 2010
Abstract
We report that AcbA is localized in vesicles that accumulate in the cortex of prespore cells just prior to sporulation. These vesicles are not observed after cells are stimulated to release AcbA but remain visible after stimulation in cells lacking the Golgi reassembly stacking protein (GRASP). Acyl...
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PMID: 20472692
PDF is available here.
PDF is available here.
Abstract
Journal of Hazardous Materials (178)1-3 2010
Abstract
A new dispersive liquid-liquid microextraction method based on solidification of floating organic drop (DLLME-SFO) combined with inductively coupled plasma-optical emission spectrometry (ICP-OES) was developed for the determination of aluminium. An appropriate mixture of acetone and 1-undecanol was...
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PMID: 20189303
PDF is available here.
PDF is available here.
Analytical Biochemistry (401)2 2010
Abstract
We present a method for isolating microsomal-type membranes from small amounts of Arabidopsis thaliana plant material that does not rely on ultracentrifugation but instead uses the lower relative centrifugal force (21,000g) of a microcentrifuge. We show that the 21,000g pellet is equivalent to that...
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PMID: 20193653
PDF is available here.
PDF is available here.
Abstract
Theriogenology (73)9 2010
Abstract
The objectives were to determine the: 1) extent of epithelial and red blood cell contamination in epididymal cat sperm samples recovered by the cutting method; 2) efficacy of simple washing, single-layer centrifugation (SLC), and swim-up for selecting epididymal cat sperm; and 3) effects of freezing...
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PMID: 20171724
PDF is available here.
PDF is available here.
Abstract
Among several effective microscopy approaches used, including sodium hypochlorite sedimentation, only some resulted in a limited increase in the laboratory workload and would be most suitable for programmatic implementation....
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PMID: 20392349
PDF is available here.
PDF is available here.
Abstract
We present a modified protocol for dematophyte diagnosis, utilizing a simple centrifugation step to significantly decrease false-negative results of the original KOH direct microscopy-based technique. Although culture constitutes the gold-standard diagnosis, the time spent for results is a limit. Fa...
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PMID: 20211663
PDF is available here.
PDF is available here.
Abstract
We apply several different methodologies, in a time-resolved manner, to follow the lifetime of such biomolecular "coronas" both in situ and isolated from the excess plasma. We find that such particle-biomolecule complexes can be physically isolated from the surrounding medium and studied in some det...
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PMID: 20356039
PDF is available here.
PDF is available here.
Abstract
We designed a method that allows for the isolation of a progenitor-enriched population of bone marrow by exploiting the physical properties of these cells. Bone marrow aspirate was separated on a discontinuous Percoll gradient (ranging from 1.050 to 1.083 g/cm3) that resulted in the recovery of six...
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PMID: 20331436
PDF is available here.
PDF is available here.
Tissue & Cell (42)2 2010
Abstract
Hypertrophic "light" and "dark" chondrocytes have been reported as morphologically distinct cell types in growth cartilage during endochondral ossification in many species, but functional differences between the two cell types have not been described. The aim of the current study was to develop a pe...
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PMID: 20303561
PDF is available here.
PDF is available here.
Abstract
The PRF protocol concentrated most platelets and leukocytes from a blood harvest into a single autologous fibrin biomaterial. This protocol offers reproducible results as long as the main production principles are respected....
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PMID: 20373539
PDF is available here.
PDF is available here.
Abstract
We study the settling process of a colloidal particle under the influence of a gravitational or centrifugal field in an unbounded electrolyte solution. Since particles in aqueous solutions normally carry a non-zero surface charge, a microscopic electric field develops which alters the sedimentation...
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PMID: 20097350
PDF is available here.
PDF is available here.
Abstract
Hydrophobic ionic liquid could be dispersed into infinite droplets under driving of high temperature, and then they can aggregate as big droplets at low temperature. Based on this phenomenon a new liquid-phase microextraction for the pre-concentration of lead was developed. In this experiment, lead...
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PMID: 20152389
PDF is available here.
PDF is available here.
Methods in Molecular Biology (632)632 2010
Abstract
We have used fluorescence-activated cell sorting (FACS) technology to purify CD133(+) and CD133(-) cellular subsets from mouse melanoma models for high-quality total RNA practical for downstream applications such as expression profiling. Implementation of this strategy can lead to higher resolution...
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PMID: 20217569
PDF is available here.
PDF is available here.
Abstract
If centrifuged lipoaspirate is used, the pellet (rich in adipose-derived mesenchymal stem cells) and the middle layer should be employed to increase fat graft survival....
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PMID: 20442104
PDF is available here.
PDF is available here.
Collegium Antropologicum (34)1 2010
Number of counting cells and cytospins selection influences on bronchoalveolar lavage cell profiles.
Abstract
Bronchoalveolar lavage (BAL) fluid cells count provides information about presence or absence of interstitial lung diseases. BAL fluid samples were taken from 50 patients hospitalized in University Hospital for Lung Diseases "Jordanovac" in Zagreb, Croatia. The samples of BAL fluid were prepared by...
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PMID: 20432749
PDF is available here.
PDF is available here.
Abstract
A suspension system for the BiVACOR biventricular assist device (BiVAD) has been developed and tested. The device features two semi-open centrifugal impellers mounted on a common rotating hub. Flow balancing is achieved through the movement of the rotor in the axial direction. The rotor is suspended...
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PMID: 19822465
PDF is available here.
PDF is available here.
Abstract
In this study, a microextraction method termed as ultrasound-assisted emulsification-microextraction (USAEME) has been developed for the extraction of organophosphorus pesticides (OPPs) in water and orange juice samples. In the USAEME method, aliquots of 50 microL chlorobenzene used...
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PMID: 20039307
PDF is available here.
PDF is available here.
Abstract
In this study, a microextraction method termed as ultrasound-assisted emulsification-microextraction (USAEME) has been developed for the extraction of organophosphorus pesticides (OPPs) in water and orange juice samples. In the USAEME method, aliquots of 50 microL chlorobenzene used...
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PMID: 20039307
PDF is available here.
PDF is available here.
Abstract
In this study, a microextraction method termed as ultrasound-assisted emulsification-microextraction (USAEME) has been developed for the extraction of organophosphorus pesticides (OPPs) in water and orange juice samples. In the USAEME method, aliquots of 50 microL chlorobenzene used...
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PMID: 20039307
PDF is available here.
PDF is available here.
Abstract
In this study, a microextraction method termed as ultrasound-assisted emulsification-microextraction (USAEME) has been developed for the extraction of organophosphorus pesticides (OPPs) in water and orange juice samples. In the USAEME method, aliquots of 50 microL chlorobenzene used...
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PMID: 20039307
PDF is available here.
PDF is available here.
Abstract
In this study, a microextraction method termed as ultrasound-assisted emulsification-microextraction (USAEME) has been developed for the extraction of organophosphorus pesticides (OPPs) in water and orange juice samples. In the USAEME method, aliquots of 50 microL chlorobenzene used...
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PMID: 20039307
PDF is available here.
PDF is available here.
Abstract
In this study, a simple, rapid and sensitive method for the determination of five phthalates including dimethyl phthalate, diethyl phthalate, dipropyl phthalate, benzyl butyl phthalate, and dicyclohexyl phthalate in fruit jellies by LC coupled with MS has been developed. Samples were...
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PMID: 19998379
PDF is available here.
PDF is available here.
Abstract
In this study, a microextraction method termed as ultrasound-assisted emulsification-microextraction (USAEME) has been developed for the extraction of organophosphorus pesticides (OPPs) in water and orange juice samples. In the USAEME method, aliquots of 50 microL chlorobenzene used...
|
PMID: 20039307
PDF is available here.
PDF is available here.
Abstract
In this study, a simple, rapid and sensitive method for the determination of five phthalates including dimethyl phthalate, diethyl phthalate, dipropyl phthalate, benzyl butyl phthalate, and dicyclohexyl phthalate in fruit jellies by LC coupled with MS has been developed. Samples were...
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PMID: 19998379
PDF is available here.
PDF is available here.
Abstract
In this study, a simple, rapid and sensitive method for the determination of five phthalates including dimethyl phthalate, diethyl phthalate, dipropyl phthalate, benzyl butyl phthalate, and dicyclohexyl phthalate in fruit jellies by LC coupled with MS has been developed. Samples were...
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PMID: 19998379
PDF is available here.
PDF is available here.
Abstract
In this study, a simple, rapid and sensitive method for the determination of five phthalates including dimethyl phthalate, diethyl phthalate, dipropyl phthalate, benzyl butyl phthalate, and dicyclohexyl phthalate in fruit jellies by LC coupled with MS has been developed. Samples were...
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PMID: 19998379
PDF is available here.
PDF is available here.
Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit (16)2 2010
Abstract
Clinical and Laboratory Standard Institute guidelines require that routine coagulation tests are performed with platelet-poor plasma (< 10,000 platelets/microL) and prepared from whole citrated blood centrifuged at low speed for 10-30 minutes. To compare results obtained from plasma centrifuged for...
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PMID: 20799581
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
With buffy coat (BC) processing of whole blood (WB) donations, the preparation of plasma occurs within 24 hours rather than 8 hours of collection. The effect of this change on coagulation factor function in plasma and cryoprecipitate was evaluated during the validation of this produc...
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PMID: 19843287
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Abstract
The persistence of naturally occurring campylobacteria in aerobic compost constructed of manure from beef cattle that were administered chlortetracycline and sulfamethazine (AS700) or from cattle not administered antibiotics (control) was examined. Although there were no differences in population si...
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PMID: 20023098
PDF is available here.
PDF is available here.
Theriogenology (73)3 2010
Abstract
The main objective of the current study was to evaluate the effects of extender type and centrifugation/washing prior to cryopreservation on the postthaw sperm parameters, lipid peroxidation, and superoxide dismutase activity of Angora buck (Capra hircus ancryrensis) sperm. Ejaculates collected from...
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PMID: 19880169
PDF is available here.
PDF is available here.
Abstract
Analytical Biochemistry (400)2 2010
Abstract
A microfluidic microarray device, which has been developed for parallel DNA detection, is now further optimized for more rapid and sensitive DNA detection and for the single-base-pair discrimination of two fungal pathogenic PCR products. Two poly(dimethylsiloxane) (PDMS)-based microf...
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PMID: 20083083
PDF is available here.
PDF is available here.
Abstract
The human umbilical cord (UC) is a biological sample that can be easily obtained just after birth. This biological sample is, most of the time, discarded and their collection does not imply any added risk to the newborn or mother s health. Moreover no ethical concerns are raised. The UC is composed...
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PMID: 20644508
PDF is available here.
PDF is available here.
Abstract
Three-dimensional (3D) cell cultures have many advantages over two-dimensional cultures. However, seeding cells in 3D scaffolds such as nonwoven fibrous polyethylene terephthalate (PET) matrices has been a challenge task in tissue engineering and cell culture bioprocessing. In this study, a centrifu...
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PMID: 19785042
PDF is available here.
PDF is available here.
Abstract
We describe a method to separate organelles by density in intact, living Drosophila embryos. Early embryos before cellularization are harvested from population cages, and their outer egg shells are removed by treatment with 50% bleach. Embryos are then transferred to a small agar plate and inserted,...
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PMID: 20613707
PDF is available here.
PDF is available here.
Abstract
I cytoskeletal pattern was lower for vitrified compared with fresh blastocysts (60.8% v. 92%, respectively; P < 0.05). The vitrified-warmed blastocysts that hatched during culture exhibited a Grade I cytoskeletal pattern. In conclusion, successful SOPS vitrification of porcine blastocysts does not r...
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PMID: 20450833
PDF is available here.
PDF is available here.
Fiziologiia cheloveka (36)3 2010
Abstract
We investigated the effect of a different amplitude of the sinusoidal translation (6 cm instead of 4 cm) and of an offset in translation (from -3 to +5 cm, instead of from -4 to +4 cm) on the parameters. Several statistical measures were used to reflect the reliability: intraclass correlation coeffi...
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PMID: 20586300
PDF is available here.
PDF is available here.
Abstract
To improve the diagnosis efficiency of human strongyloidiasis by using formalin-ether concentration technique (FECT), the effects of various factors on the recovery rates of Strongyloides stercoralis larvae were comparatively evaluated. Fresh stool and a short time exposure of larvae to formalin yie...
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PMID: 19923489
PDF is available here.
PDF is available here.
Abstract
We describe the development of a fully-automated solution-phase synthesizer, 'ChemKonzert' that can be used to prepare a wide variety of organic compounds. The automated synthesizer is ingeniously designed to perform most of the chemical reactions currently used by synthetic organic chemists and uti...
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PMID: 20045972
PDF is available here.
PDF is available here.
Arzneimittel-Forschung (60)10 2010
Abstract
These results suggest that blood samples for ribavirin analysis can be sent at room temperature within a period of 2 h between sampling and centrifugation....
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PMID: 21125815
PDF is available here.
PDF is available here.
Abstract
A centrifugal microanalysis system using spectrophotometric detection has been developed for the determination of nitrite and chromium VI. The system was designed to illustrate the potential of centrifugal microfluidic systems for on-site (field) analysis of waters. The performance was significantly...
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PMID: 19836536
PDF is available here.
PDF is available here.
Analytical Biochemistry (398)2 2009
Abstract
We compared four combinations of alterations to classical nuclear isolation methods as follows. Mouse liver was gently crushed through a fine mesh with and without in vivo perfusion with collagenase. The cell suspension was centrifuged at 600 x g to remove gross debris, then at moderate centrifugal...
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PMID: 19925772
PDF is available here.
PDF is available here.
Abstract
We determined the subcellular distribution of soluble carbohydrates by combining nonaqueous fractionation method and infiltration-centrifugation method. During petal growth, fructose and glucose rapidly accumulated in the vacuole, reaching a maximum when petals almost reflected. Transmission electro...
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PMID: 20183924
PDF is available here.
PDF is available here.