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Bacteriological Techniques (2):
Recent article
Bacteriological Techniques
Abstract
We show that this QS system plays a major role in the control of S. pneumoniae biofilm formation. Our results demonstrate that the luxS gene is contained by invasive isolates and normal-flora strains in a region that contains genes involved in division and cell wall biosynthesis. The luxS gene was m...
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PMID: 21825061
PDF is available here.
PDF is available here.
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Abstract
Rhodospirillum rubrum was grown under light anaerobic conditions with phycocyanin (C-pc) extracted from Spirulina platensis as the sole source of carbon and nitrogen. When grown under these conditions cellular components like lipids, carbohydrates, protein, carotenoids, bacteriochlor...
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PMID: 22013744
PDF is available here.
PDF is available here.
The Lancet (378)9790 2011
Abstract
The Lancet (378)9790 2011
Abstract
The Lancet (378)9790 2011
Abstract
Therapeutische Umschau (68)7 2011
Abstract
Unexplained cough for more than 2 - 3 weeks or patients considered to be at high risk for tuberculosis (TB) should be investigated in a timely manner: Chest radiography and 3 sputum examinations for detection acid-fast bacilli and TB cultures are essential first diagnostic steps. Pending sputum resul...
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PMID: 21728154
PDF is available here.
PDF is available here.
Abstract
New England Journal of Medicine (364)22 2011
Abstract
We compared real-time PCR assays of liquid-saliva and dried-saliva specimens with rapid culture of saliva specimens obtained at birth.
A total of 177 of 34,989 infants (0.5%; 95% confidence interval [CI], 0.4 to 0.6) were positive for CMV, according to at least one of the three metho...
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PMID: 21631323
PDF is available here.
PDF is available here.
Abstract
Abstract
A possible role of metabolism by intestinal bacteria in arbutin-induced toxicity was investigated in mammalian cell cultures. Following an incubation of arbutin with intestinal bacteria, either Bifidobacterium longum HY81 or Bifidobacterium adolescentis, for 24 h, its aglycone hydroquinone could be p...
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PMID: 21544735
PDF is available here.
PDF is available here.
Abstract
In developing countries like ours with a large number of tuberculosis (TB) cases and limited resources, the diagnosis of TB relies primarily on smear microscopy for Acid Fast Bacilli (AFB) but its sensitivity is limited in paucibacillary cases.
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PMID: 21644393
PDF is available here.
PDF is available here.
Abstract
We developed a microbiology data warehouse for networking hospital laboratories in Akita prefecture. This system, named Akita-ReNICS, is an easy-to-use information management system designed to compare, track, and report the occurrence of antimicrobial-resistant organisms. Participating laboratories...
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PMID: 21626877
PDF is available here.
PDF is available here.
Abstract
To describe our own experience with pyogenic liver abscesses over the past 10 years and investigate the risk factors associated with failure of initial percutaneous therapy.
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PMID: 21472130
PDF is available here.
PDF is available here.
Abstract
We present here data for the spread of Pseudomonas fluorescens caused by a contaminated drinking water dispenser in a bone marrow transplant unit. Over a 1-month period we observed a sharp increase in the isolation of P. fluorescens from weekly pharyngeal surveillance swabs. Environmental samples we...
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PMID: 21450958
PDF is available here.
PDF is available here.
Abstract
Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production. The aim of this work was: i...
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PMID: 21526657
PDF is available here.
PDF is available here.
Abstract
Streptococcus agalactiae is a significant worldwide cause of morbidity and mortality in pregnant women and their newborn infants. The objective of this work was to determine the usefulness of bioMrieux chromogenic medium chromID Strepto B (CR) for detecting S. agalactiae in pregnant women from the se...
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PMID: 21491059
PDF is available here.
PDF is available here.
Medicina Intensiva (35)2 2011
[Microbiological profile of infections in the Intensive Care Units of Colombia (EPISEPSIS Colombia].
Abstract
Valid and reliable data regarding sepsis is lacking in Colombia. Our aim was to determine the prevalence of the microorganisms in the main infections treated in Intensive Care Units (ICUs) in our country.
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PMID: 21334104
PDF is available here.
PDF is available here.
Abstract
This unit describes a series of in vivo/ex vivo combined protocols for investigating the interactions (adhesion, phagocytosis, and killing) of extracellular bacteria with peritoneal murine macrophages. It includes steps needed for in vivo infection of murine peritoneal macrophages af...
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PMID: 21400679
PDF is available here.
PDF is available here.
Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation (9)1 2011
Meningoencephalitis with Aspergillus and mycobacterium tuberculosis in a renal transplant recipient.
Abstract
Broad-spectrum antibiotics, antituberculous agents, and amphotericin were started. Unfortunately, the woman's condition deteriorated, and she died 2 weeks after admission to the hospital....
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PMID: 21605027
PDF is available here.
PDF is available here.
Abstract
The purpose of the investigation was to estimate the content of malondialdehyde (MDA) in the coprofiltrates of human beings with bacteriologically verified colonic dysbacteriosis and to clarify a relationship of the amount of MDA in the coprofiltrates to the degree of the abnormal qualitative and qua...
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PMID: 21506382
PDF is available here.
PDF is available here.
Abstract
The highly antagonistic lactobacillus strains isolated from the oral cavity of human individuals were genetically passported as L. fermentum 39, L. rhamnosus 50, and L. rhamnosus 24, by applying the RAPD-PCR technique with two types of primers (M13, MSP). These lactobacillus strains showed high degre...
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PMID: 21506386
PDF is available here.
PDF is available here.
Abstract
We describe a strategy, termed in vivo microbial antigen discovery (InMAD), that we used to identify circulating bacterial antigens. This technique starts with "InMAD serum," which is filtered serum that has been harvested from BALB/c mice infected with a bacterial pathogen. The InMAD serum, which i...
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PMID: 21846829
PDF is available here.
PDF is available here.
Abstract
Clostridium difficile continues to cause infections in healthcare and other settings. Its spores survive well indoors and require sporicidal chemicals for infection control. However, proper testing of disinfectants is impeded due to difficulties in obtaining viable spores of high enough quality and t...
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PMID: 21563698
PDF is available here.
PDF is available here.
Abstract
We investigate the technical basis of a previous study as reported by Nieselt et al. (BMC Genomics 11:10, 2010) in more detail, based on the same samples and focusing on the validation of the custom-designed microarray as well as on the reproducibility of the data generated from biological replicate...
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PMID: 21431541
PDF is available here.
PDF is available here.
General dentistry (59)1 2011
Abstract
The clinical failure of coronal restorations can compromise the healthy periapical status of endodontically treated teeth. The purpose of the present ex vivo study was to assess the effectiveness of the cervical barrier in preventing bacterial microleakage in the eventual loss of the...
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PMID: 21613029
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)12 2010
Abstract
We enrolled adults undergoing chest radiography at three Indian Health Service clinics in the Southwestern United States and collected acute and convalescent serum for measurement of PsaA and PspA titres and urine for pneumococcal antigen detection. Blood and sputum cultures were obtained at the dis...
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PMID: 20334727
PDF is available here.
PDF is available here.
Lab On a Chip (10)22 2010
Abstract
Our approach yields a detection limit of 800 cells mL(-1), one of the highest-sensitivity immunosensors to date....
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PMID: 20862443
PDF is available here.
PDF is available here.
Abstract
We report the current knowledge about the sRNAs of these socioeconomically relevant Gram-positive pathogens, overview the state-of-the-art high-throughput sRNA screening methods and summarize bioinformatics approaches for genome-wide sRNA identification and target prediction. Finally, we discuss the...
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PMID: 21255362
PDF is available here.
PDF is available here.
Lancet Infectious Diseases (10)11 2010
Abstract
Abstract
We reevaluated the accuracy of antenatal group B streptococcal (GBS) culture results in predicting colonization at the time of delivery in a general practice setting. Patients who had late third-trimester antenatal GBS cultures were prospectively identified. A repeat GBS culture was performed when a...
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PMID: 20458663
PDF is available here.
PDF is available here.
Abstract
We collected a total of 1000 urine samples, and 325 of these samples tested positive for a UTI via traditional culturing techniques; additionally, all 325 of these samples tested positive with the Eub338 probe in FISH analysis. FISH analyses with species-specific probes were performed in parallel to...
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PMID: 20807557
PDF is available here.
PDF is available here.
Abstract
We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activ...
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PMID: 20801170
PDF is available here.
PDF is available here.
Abstract
In the light of the poor culturability of Acidobacteria and Verrucomicrobia species, group-specific real-time (qPCR) systems were developed based on the 16S rRNA gene sequences from culturable representatives of both groups. The number of DNA targets from three different groups, i.e. Holophagae (Aci...
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PMID: 20801169
PDF is available here.
PDF is available here.
Abstract
In this paper, a sensitive immunoassay method was proposed for Listeria monocytogenes detection by using highly fluorescent bioconjugated nanoparticles probe. (FITC-IgG)-doped fluorescent silica nanoparticles (fsNPs) firstly were synthesized by a microemulsion method and characterized by TEM and flu...
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PMID: 20807555
PDF is available here.
PDF is available here.
Abstract
: In cataract surgery, repeated irrigation of the operative field with povidone-iodine at a concentration of 0.25% achieved an extremely low bacterial contamination rate in the anterior chamber at the completion of surgery.
Copyright © 2010 Elsevier Inc. All rights reserved....
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PMID: 20970112
PDF is available here.
PDF is available here.
Abstract
The human immunodeficiency virus (HIV) alters the presentation of pulmonary tuberculosis (PTB), but it remains unclear whether alterations occur at a CD4 cell threshold or throughout HIV infection.
To better understand the relationship between CD4 count and clinical and radiographic presentation of...
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PMID: 20843421
PDF is available here.
PDF is available here.
Abstract
Our results indicate that PCR amplification and sequencing of the mycobacterial hsp65 gene is a sensitive assay for identification of NTM species in FFPE materials. However, consistent results of PCR analysis, microbiology study, histologic manifestations, radiology, and clinical presentation are im...
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PMID: 20850247
PDF is available here.
PDF is available here.
Proteomics (10)17 2010
Abstract
We optimized a proteolytic shaving approach to identify those surface-exposed protein domains - the surfacome - of S. aureus that are accessible to extracellular bio-macromolecules, for example in the host milieu. Subsequently, this approach was applied to define the surfacomes of four strains with...
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PMID: 20662103
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to evaluate a fast and simple bead-based method using paramagnetic beads covered with recombinant Listeria phage endolysin-derived cell-wall-binding domain proteins specific for Listeria spp. for separation of the foodborne pathogen Listeria monocytogenes fr...
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PMID: 20500083
PDF is available here.
PDF is available here.
Abstract
A microsphere-based multiplex assay was developed, by using the xMAP technology, for the simultaneous rapid detection of the Mycobacterium tuberculosis complex (MTC) and the differentiation of M. tuberculosis and M. bovis. The assay simultaneously detected 4 target sequences, including specific inse...
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PMID: 20736748
PDF is available here.
PDF is available here.
Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit (16)9 2010
Abstract
The mode of the transmission of Helicobacterpylori infection remains poorly understood. A total of 198 samples of drinking water from 22 districts of Basra governorate were collected during the period October 2006 to July 2007. The concentration of residual chlorine was measured and the numbers of t...
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PMID: 21218716
PDF is available here.
PDF is available here.
Journal of Maternal-Fetal and Neonatal Medicine (23 Suppl 2) 2010
Abstract
Abstract
Food Microbiology (27)6 2010
Abstract
This study provides an overview and criteria for the selection of a method, other than the reference method, for microbial analysis of foods. In a first part an overview of the general characteristics of rapid methods available, both for enumeration and detection, is given with reference to relevant...
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PMID: 20630313
PDF is available here.
PDF is available here.
Abstract
Just over one-third of sepsis patients have positive blood cultures, mainly due to inadequate sampling volumes (50% of adults have < 1.0 CFU/mL blood) and the prior use of antibiotics. However, 20-30% of sepsis patients are given inappropriate empirical antibiotics. The Clinical and Laboratory Stand...
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PMID: 20688297
PDF is available here.
PDF is available here.
Abstract
A retrospective analysis of six patients diagnosed with brucellosis in two regional hospitals was carried out. The epidemiological, clinical, and laboratory features were studied. All patients had exposure history. Three patients presented with musculoskeletal symptoms, while three had predominantly...
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PMID: 20683074
PDF is available here.
PDF is available here.
Abstract
We (1) compared the frequency of positive cultures with synovial fluid inoculated in blood culture flasks (SF) with those of periprosthetic tissues or swabs in traditional cultures from patients with acute and chronic prosthetic joint infections (PJI) and (2) determined the sensitivity, specificity,...
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PMID: 20162386
PDF is available here.
PDF is available here.
Abstract
We (1) asked whether the clinical indicators could predict laboratory findings during the initial stage of Vibrio necrotizing fasciitis and (2) determined the relationships between the laboratory risk indicator for necrotizing fasciitis (LRINEC) score and the diagnosis of Vibrio infection....
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PMID: 20232179
PDF is available here.
PDF is available here.
Critical Care Medicine (38)8 Suppl 2010
Abstract
Despite recent advances in antifungal treatments, the morbidity and mortality of fungal infections, especially invasive candidiasis, in patients in the intensive care unit setting remain high. Because of this, there has been a great interest in improving the evaluation, risk assessment, and preventi...
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PMID: 20647796
PDF is available here.
PDF is available here.
Abstract
The effects of chemotactic stimuli on motility ability of viable Campylobacter to pass through a 0.45 microm pore size filter in viscous condition were investigated. Reference strains including C. jejuni ATCC 33291, C. coli MUMT 18407, C. lari ATCC 43675, and C. upsaliensis DMST 19055 were used. The...
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PMID: 20561959
PDF is available here.
PDF is available here.
Abstract
Wild type T4 bacteriophage and recombinant T4 bacteriophages displaying biotin binding peptide (BCCP) and cellulose binding module (CBM) on their heads were immobilized on nano-aluminum fiber-based filter (Disruptor), streptavidin magnetic beads and microcrystalline cellulose, respectively. Infectiv...
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PMID: 20561957
PDF is available here.
PDF is available here.
Abstract
An external quality assessment (EQA) panel consisting of a total of 13 samples in broncho alveolar lavage (BAL) or transport medium was prepared to assess the proficiency of laboratories in the correct detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by nucleic acid amplification tech...
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PMID: 20493214
PDF is available here.
PDF is available here.
Abstract
We were able to differentiate all Borrelia species and their subtypes within particular species. RT-PCR based on Tm determination identified unique strains within the species Borreliaafzelii (Tm 66.11 degrees C), B. burgdorferi sensu stricto (Tm 68.18 degrees C), Borreliaspielmanii (Tm 59.45 degrees...
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PMID: 20451566
PDF is available here.
PDF is available here.
Abstract
In the last few decades, molecular typing has become an important tool in taxonomic, phylogenetic and identification studies of numerous groups of bacteria, including the yersiniae. In this study, Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR), Pulsed-Field Gel Electrophoresis (PFGE)...
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PMID: 20493215
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (309)1 2010
Abstract
Molecular ecology methods are now well established for the culture-independent characterization of complex bacterial communities associated with various environmental and animal habitats and are revealing the extent of their diversity. By comparison, it has become clear that only a s...
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PMID: 20487025
PDF is available here.
PDF is available here.
Abstract
Toxic cyanobacterial blooms, as well as their increasing global occurrence, pose a serious threat to public health, domestic animals, and livestock. In Missisquoi Bay, Lake Champlain, public health advisories have been issued from 2001 to 2009, and local microcystin concentrations found in the lake...
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PMID: 20562282
PDF is available here.
PDF is available here.
Abstract
A microbial diagnostic microarray for the detection of the most relevant bacterial food- and water-borne pathogens and indicator organisms was developed and thoroughly validated. The microarray platform based on sequence-specific end labelling of oligonucleotides and the pyhylogeneti...
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PMID: 21255342
PDF is available here.
PDF is available here.
Abstract
Abstract
There is an increasing demand for composite resins with both strong antibacterial activity and satisfactory mechanical properties. This study tested the hypothesis that the new antibacterial agent tetrapod-like zinc oxide whisker (T-ZnOw) could simultaneously enhance the antibacterial activity and m...
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PMID: 20439932
PDF is available here.
PDF is available here.
Abstract
We assessed the utility of reporting direct blood Gram stain results compared with the results of positive blood cultures in 482 episodes, and monitored the impact on the selection of antimicrobial treatment. We found that the groups: "Staphylococcus species (spp.)", "Pseudomonas spp. and related or...
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PMID: 20715512
PDF is available here.
PDF is available here.