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Microbiological Techniques (9):
Recent article
Microbiological Techniques
Clinical Microbiology and Infection (17 Suppl 4) 2011
Abstract
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Abstract
We report here a case of disseminated skin infection caused by Mucor velutinosus, a recently described new species. We believe this to be the first published report of a clinical case of mucormycosis due to M. velutinosus, as well as a rare case of dissemination from a deep site to skin.
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PMID: 21543575
PDF is available here.
PDF is available here.
Abstract
The central dialysis fluid delivery system (CDDS), with which dialysis fluid is prepared at a single location and sent to each patient station, was developed as a unique system of dialysis in Japan and has been widely used. Maintenance hemodialysis using the single patient dialysis machine (SPDM), w...
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PMID: 20938129
PDF is available here.
PDF is available here.
Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances & Environmental Engineering (45)14 2010
Abstract
This study evaluated the kinetics of 2,6-dihydroxybenozic acid and peptone biodegradation at low sludge age by acclimated culture under aerobic conditions. A laboratory-scale sequencing batch reactor was set and fed with peptone mixture. The system was operated at steady-state at a sludge age of 2 d...
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PMID: 20981603
PDF is available here.
PDF is available here.
Trends in Microbiology (18)12 2010
Abstract
We believe are the current priority research areas in assessing microbial risk to paper heritage, and reports studies on a 13th century Italian manuscript and on Leonardo da Vinci's Atlantic Codex which illustrate the problems and challenges encountered when dealing with microbial investigations of...
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PMID: 20971645
PDF is available here.
PDF is available here.
Biocontrol Science (15)4 2010
Abstract
Our data reveal that the new method considerably shortens the time required for detection of yeasts and molds in food....
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PMID: 21212506
PDF is available here.
PDF is available here.
Semi-automated extraction of microbial DNA from feces for qPCR and phylogenetic microarray analysis.
Abstract
We combined mechanical disruption of microbial cells by repeated bead-beating (RBB) with two automated DNA extraction methods, KingFisher with InviMag Stool DNA kit (KF) and NucliSENS easyMAG (NeM). The semi-automated DNA extraction methods, RBB combined with either KF or NeM, were compared to the m...
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PMID: 20849891
PDF is available here.
PDF is available here.
Epidemiology (21)5 2010
Abstract
We used latent class analysis to estimate the proportion of pneumonia episodes caused by pneumococcus. Furthermore, we extended this methodology to evaluate the effect of antimicrobial treatment on test accuracies and the prevalence of the disease. The study combined data from 5 validation studies o...
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PMID: 20562627
PDF is available here.
PDF is available here.
Methods in Molecular Biology (583)583 2010
Abstract
We discuss the sensitivity of the system with regard to the impedance sensor and describe the optical setup. The relevant parameters related to the experimental setup and sample preparation are discussed. The use of dielectrophoretic forces for particle manipulation is presented as a simple enabling...
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PMID: 19763464
PDF is available here.
PDF is available here.
Journal of Biophotonics (3)8-9 2010
Abstract
Abstract
The paper discusses the name "dysbacteriosis" as a microbiological, ecological, and clinical term.
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PMID: 20886721
PDF is available here.
PDF is available here.
Infection and Immunity (78)7 2010
Abstract
We have developed an alternative, low-cost, and easy-to-use infection model for Aspergillus species based on embryonated eggs. The outcome of infections in the egg model is dose and age dependent and highly reproducible. We show that the age of the embryos affects the susceptibility to A. fumigatus...
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PMID: 20421382
PDF is available here.
PDF is available here.
Abstract
The objective was to determine the occurrence of four urethral pathogens in urine specimens from symptomatic men using transcription mediated amplification (TMA) assay. Urethral swab and urine specimens from 300 men presenting to a family practitioner were Gram stained and tested for Neisseria gonor...
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PMID: 20852197
PDF is available here.
PDF is available here.
Abstract
Of clinics responding to the audit, 99 and 97% have policies that are compliant with the British Association for Sexual Health and HIV National Guidelines for testing of asymptomatic men and women for HIV and syphilis, respectively. All clinics offer men, and all but one clinic offer women, screenin...
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PMID: 20852203
PDF is available here.
PDF is available here.
Abstract
I have tackled these issues. Not only laboratory performance and activity, but the information system for healthcare workers were significantly improved. As a result, appropriate antimicrobial use has been achieved in part....
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PMID: 20715517
PDF is available here.
PDF is available here.
Abstract
Procalcitonin (PCT) is synthesized by a large number of tissues and organs in response to invasion by pathogenic bacteria, fungi, and some parasites. Current PCT assays are rapid, specific, and of sufficient sensitivity to detect increases in PCT serum levels within 4 to 6 h of initi...
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PMID: 20421436
PDF is available here.
PDF is available here.
Abstract
The still poorly explored world of microbial functioning is about to be uncovered by a combined application of old and new technologies. Bacteria, especially, are still in the dark with respect to their phylogenetic affiliations as well as their metabolic capabilities and functions....
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PMID: 20337722
PDF is available here.
PDF is available here.
Abstract
The diversity and antifungal resistance of yeasts able to grow at 37 degrees C and the occurrence of bacterial indicators of water quality were studied in three lakes in Southeastern Brazil. The densities of yeasts, Escherichia coli, Enterococcus spp. and Pseudomonas aeruginosa were determined by th...
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PMID: 20154391
PDF is available here.
PDF is available here.
Abstract
In this study, we found that the PCR technique has applicability in the study of cagA, and other genes related to the H. pylori pathogen. This method can be applied to samples directly from biopsy or isolated from the bacteria....
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PMID: 19693671
PDF is available here.
PDF is available here.
Abstract
Our finding of a potential pathogen in 57/116 (49%) of our cases, although not necessarily the cause of death, confirms the relevance of performing multisite and virology investigations in all cases of SUDI. Standardised protocols with agreed definitions are necessary for a consistent approach....
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PMID: 20457701
PDF is available here.
PDF is available here.
Abstract
Abstract
Anidulafungin, micafungin, and caspofungin in vitro activities against Candida metapsilosis, C. orthopsilosis, and C. parapsilosis were evaluated by MICs and time-kill methods. All echinocandins showed lower MICs (mean MICs, 0.05 to 0.71 mg/liter) and the highest killing rates (-0.06 to -0.05 CFU/ml...
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PMID: 20145083
PDF is available here.
PDF is available here.
Abstract
This study assessed the spectrum of activity of torezolid (TR-700), the active moiety of torezolid phosphate (TR-701), and proposes tentative MIC and disk diffusion breakpoints as well as quality control ranges. The in vitro susceptibilities of 1,096 bacterial isolates, representing 23 different spe...
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PMID: 20231392
PDF is available here.
PDF is available here.
Abstract
We sequenced katG of 108 INH-resistant M. tuberculosis clinical isolates. Consequently, 9 novel KatG mutants with a single-amino-acid substitution were found. All of these mutants had significantly lower INH oxidase activities than the wild type, and each mutant showed various levels of activity. Is...
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PMID: 20211896
PDF is available here.
PDF is available here.
Abstract
We reported a novel strategy of "targeted killing" through the design of narrow-spectrum molecules known as specifically targeted antimicrobial peptides (STAMPs) (R. Eckert et al., Antimicrob. Agents Chemother. 50:3651-3657, 2006; R. Eckert et al., Antimicrob. Agents Chemother. 50:1480-1488, 2006)....
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PMID: 20211885
PDF is available here.
PDF is available here.
Abstract
We have adapted and tested this technology for use with blood culture broths, thus allowing identification in less than 30 min once the blood culture is detected as positive. Our method is based on the selective recovery of bacteria by adding a detergent that solubilizes blood cells but not microbia...
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PMID: 20237092
PDF is available here.
PDF is available here.
Abstract
Genome shuffling based on cross mating was used to improve the tolerance of the pentose-fermenting yeast Pichia stipitis towards hardwood spent sulphite liquor (HW SSL). Six UV-induced mutants of P. stipitis were used as the starting strains, and they were subjected to 4 rounds of genome shuffling....
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PMID: 20298725
PDF is available here.
PDF is available here.
Abstract
In a direct comparison with established methods for Phytophthora ramorum detection (isolation followed by morphological identification, or conventional DNA extraction followed by TaqMan real-time PCR) a rapid, simplified detection method in which membranes of lateral flow devices (LFDs) are added di...
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PMID: 20171248
PDF is available here.
PDF is available here.
Health Policy (95)2-3 2010
Abstract
General performance of MC was poor. In 2006, the high and low performance groups checked on average 190 and 24 TB suspects, respectively. The majority of TB suspects who visited a MC did not have their sputum checked, or sputum was checked but the result was not recorded. TB suspects who visited a M...
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PMID: 20004490
PDF is available here.
PDF is available here.
Abstract
We investigated the survival mechanisms of freeze-dried or liquid-dried (L-dried) yeast cells in ampoules. Type strains of various yeasts were freeze-dried or L-dried and sealed in ampoules under high vacuum (< 1 Pa) or low vacuum (4.8 x 10(4) Pa), then stored at 37 degrees C (accelerated storage te...
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PMID: 20513958
PDF is available here.
PDF is available here.
Abstract
Journal of Biochemistry (147)4 2010
Abstract
Esmond E. Snell (1914-2003) was a giant of B-vitamin and enzyme research. His early research in bacterial nutrition had lead to the discovery of vitamins such as lipoic acid and folic acid, and an anti-vitamin avidin. He developed microbiological assay methods for riboflavin and other vitamins and a...
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PMID: 20353954
PDF is available here.
PDF is available here.
Clinical Microbiology and Infection (16 Suppl 2) 2010
Abstract
Abstract
Diabetic foot infections can be a challenge to diagnose, especially when osteomyelitis is in question. Evaluation of infection should involve a thorough examination of the extremity for clinical signs of infection along with appropriate laboratory and imaging studies. Laboratory mark...
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PMID: 20207620
PDF is available here.
PDF is available here.
Abstract
Diabetic foot infections can be a challenge to diagnose, especially when osteomyelitis is in question. Evaluation of infection should involve a thorough examination of the extremity for clinical signs of infection along with appropriate laboratory and imaging studies. Laboratory mark...
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PMID: 20207620
PDF is available here.
PDF is available here.
Abstract
Foot wounds are the most common diabetes-related cause of hospitalization and frequently result in amputation. Although generally diagnosed clinically based on signs and symptoms of inflammation, empirical antibiotic treatment should be based on tissue cultures until resolution of in...
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PMID: 20207621
PDF is available here.
PDF is available here.
Abstract
Foot wounds are the most common diabetes-related cause of hospitalization and frequently result in amputation. Although generally diagnosed clinically based on signs and symptoms of inflammation, empirical antibiotic treatment should be based on tissue cultures until resolution of in...
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PMID: 20207621
PDF is available here.
PDF is available here.
Pneumologie (64)3 2010
Abstract
The frequency and range of bacterial pathogens in respiratory tract infections depends on a variety of variables. The aetiology is influenced by the type of respiratory tract infection (acute bronchitis, acute exacerbation of chronic bronchitis, community-acquired pneumonia, nosocomial pneumonia), g...
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PMID: 19918719
PDF is available here.
PDF is available here.
Abstract
The blood culture method using the BacT/Alert system is useful for culturing dialysates and improves the positive culture rate in patients with suspected peritonitis compared to the conventional culture method....
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PMID: 20195408
PDF is available here.
PDF is available here.
Collegium Antropologicum (34)1 2010
Abstract
Medical Journal of Australia (192)4 2010
Abstract
The Nurse Practitioner (35)2 2010
Abstract
Abstract
We have identified as the product of TK1827. This enzyme (pTK1827) has been purified and shown to hydrolyze glucopyranoside but not mannopyranoside, have optimal activity at 95 degrees C and from pH 8 to 9.5, and have a functional half-life of approximately 7 min at 100 degrees C. To generate a stra...
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PMID: 20023088
PDF is available here.
PDF is available here.
Abstract
We developed a nonhuman primate model of CA-MRSA pneumonia and used it to test the hypothesis that PVL contributes to lower respiratory tract infections caused by S. aureus strain USA300. The lower respiratory tract disease observed in this monkey model mimicked the clinical and pathological feature...
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PMID: 20093487
PDF is available here.
PDF is available here.
Abstract
We demonstrate how simple microfluidic devices can be used to visualize microbial interactions with micro-structured habitats, to identify key processes influencing the observed phenomena, and to systematically validate predictive models. Simple, easy-to-use flow cells were constructed out of the tr...
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PMID: 20440259
PDF is available here.
PDF is available here.
Abstract
Routinely applied efforts to diagnose congenital infections in cases presenting with LSV have a poor yield. Routine TORCH screening in neonates with LSV cases should only be regarded as mandatory once well-designed studies demonstrate a clear diagnostic benefit.
Copyright 2009 S. Karger AG, Basel....
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PMID: 19887856
PDF is available here.
PDF is available here.
Abstract
Our rates of missed test results are lower than those reported from studies where paper ordering and reporting systems were used. This suggests that the availability of CPOE systems may reduce the risk of these events. Electronic result delivery, with electronic endorsement to allow documentation of...
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PMID: 19893851
PDF is available here.
PDF is available here.
Iranian Biomedical Journal (14)1-2 2010
Abstract
Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical char...
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PMID: 20683498
PDF is available here.
PDF is available here.
Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology (Suppl) 2010
Abstract
Abstract
Abstract
Abstract
Clinical microbiology is a specific combination of knowledge, attitude and practice aimed at direct clinical involvement in infectious disease management using the core principles of medical microbiology and clinical medicine. In this article certain areas in microbiology, where a more proactive app...
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PMID: 20551520
PDF is available here.
PDF is available here.
Abstract
We review the recent development of AFM applications in the microbial area. We discuss several approaches for using AFM scanning images to investigate morphological characteristics of microbes and the use of force-distance curves to investigate interaction of microbial samples at the nanometer and c...
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PMID: 20695025
PDF is available here.
PDF is available here.
Abstract
We present the fascinating options offered by the rapid advances in AFM with emphasizes on bacterial research and provide a background for the exciting research articles to follow.
2010 Wiley Periodicals, Inc....
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PMID: 20695026
PDF is available here.
PDF is available here.
Abstract
We identified patients from two large academic hospital emergency departments with suspected sepsis. The results of a multiplex PCR that could detect 25 bacterial and fungal pathogens were compared to those of blood culture. The results were analyzed with respect to the likelihood of infection, seps...
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PMID: 19846634
PDF is available here.
PDF is available here.
Abstract
In Czech Republic, clinical microbiology laboratories are currently acredited in accordance with the ISO 15189 standard specifying requirements for quality management and tracing of diagnostic device batches used in individual (albeit partial) tests. At present, practically all laboratories are equi...
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PMID: 20411625
PDF is available here.
PDF is available here.
Pacific health dialog : a publication of the Pacific Basin Officers Training Program and the Fiji School of Medicine (15)2 2009
Abstract
Transfer of viable organisms from patient's mouths was found on the compound at all stages of the impression process. Improper disinfection and storage of impression compound and trays allowed for the introduction of hospital pathogens on the compound that were not initially present from the patient...
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PMID: 20443519
PDF is available here.
PDF is available here.
Abstract
Abstract
Bacteriocin, an antimicrobial agent having potential for food biopreservation was purified from Lactobacillus brevis (a safe food-grade bacteria isolated from Vari Kandal, a traditional fermented food of Himachal Pradesh by adopting a novel repeated washing method. Its purity was confirmed by SDS-PA...
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PMID: 19788067
PDF is available here.
PDF is available here.
Bosnian journal of basic medical sciences / Udruzenje basicnih mediciniskih znanosti = Association of Basic Medical Sciences (9)3 2009
Evaluation and importance of selected microbiological methods in the diagnosis of human brucellosis.
Abstract
We compared the sensitivity among test-methods: Rose Bengal test-100.0%, blood culture-30.8%, ELISA IgM-64.8% and ELISA IgG-56.1%. Sensitivity of test methods was different in the different stages of illness. It is necessary to use combination of different tests such are blood culture, Rose Bengal t...
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PMID: 19754473
PDF is available here.
PDF is available here.