Abstract
We showed that the highly conserved Psa fimbriae of Y. pestis (also called pH 6 antigen) are expressed in murine organs after infection through the respiratory tract. Studies with iron dextran-treated mice showed that vaccination with the Psa fimbrial protein together with an adjuvant afforded incom...
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PMID: 20498260
PDF is available here.
Abstract
Growth was seen in one culture with one of the procedures used. The molecular characterization did not correspond to the initially analyzed isolate, and therefore was deduced to be the product of a cross-contamination. CONCLUSION: The DNA extraction protocol, as described by van Soolingen, et al. 20...
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PMID: 20440455
PDF is available here.
Abstract
Scenarios where the results of well-intentioned scientific research can be used for both good and harmful purposes give rise to what is now widely known as the 'dual-use dilemma'. There has been growing debate about the dual-use nature of life science research with implications for making biological...
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PMID: 19784453
PDF is available here.
Abstract
The present study investigates the axial dispersion and retardation patterns of viruses in a pressurized water distribution pipe using MS-2 as a surrogate. The results were obtained by using computational fluid dynamics (CFD), along with a hydraulic and water quality model. These models included the...
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PMID: 19827488
PDF is available here.
Abstract
A major challenge in influenza research is the selection of an appropriate animal model that accurately reflects the disease and protective immune response to influenza infection in humans. Ferrets are exquisitely susceptible to infection with human influenza viruses and are widely believed to be th...
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PMID: 19412910
PDF is available here.
Abstract
A major challenge in influenza research is the selection of an appropriate animal model that accurately reflects the disease and protective immune response to influenza infection in humans. Ferrets are exquisitely susceptible to infection with human influenza viruses and are widely believed to be th...
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PMID: 19412911
PDF is available here.
Abstract
This unit, in conjunction with local and national guidelines and regulations (see APPENDIX 1B), provides the basic biosafety information needed to perform the procedures detailed in this manual. Topics discussed include routine precautions when working with biohazards, disinfectants, disposal of bio...
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PMID: 19412909
PDF is available here.
Abstract
We aimed to identify the meanings constructed about occupational risk by health workers through the structural approach to social representation. 220 health professionals from a public hospital in Natal, Rio Grande do Norte, Brazil, participated in this research. The technique of free evocation of w...
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PMID: 19653562
PDF is available here.
Abstract
We developed a henipaviral entry assay based on a beta-lactamase-Nipah Matrix (betala-M) fusion protein. We first codon-optimized the bacterial betala and the NiV-M genes to ensure efficient expression in mammalian cells. The betala-M construct was able to bud and form virus-like particles (VLPs) th...
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PMID: 19646266
PDF is available here.
Abstract
The present computer-assisted biohazard safety cabinet for preparation of the mixture of anticancer agents is considered to be potentially useful for the safe management in cancer chemotherapy.
Copyright 2009 S. Karger AG, Basel....
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PMID: 19468221
PDF is available here.
Abstract
Last week, the U.S. Department of Homeland Security selected Kansas State University in Manhattan as its preferred site for a $450 million facility to replace the aging Plum Island Animal Disease Center off Long Island, New York.
PDF is available here.
James W Le Duc,
Kevin Anderson,
Marshall E Bloom,
James E Estep,
Heinz Feldmann,
Joan B Geisbert,
Thomas W Geisbert,
Lisa Hensley,
Michael Holbrook,
Peter B Jahrling,
Thomas G Ksiazek,
George Korch,
Jean Patterson,
John P Skvorak and
Hana Weingartl
Abstract
Construction of several new Biosafety Level 4 (BSL-4) laboratories and expansion of existing operations have created an increased international demand for well-trained staff and facility leaders. Directors of most North American BSL-4 laboratories met and agreed upon a framework for leadership and t...
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PMID: 18976549
PDF is available here.
Abstract
The Biodefense and Emerging Infections Research Resources Repository (BEI Resources) provides unique, quality-assured reagents to the scientific community for use in basic research and product development involving biodefense and emerging infectious diseases. These include microorganisms (up to Bios...
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PMID: 18675849
PDF is available here.
Abstract
We have devised and constructed a biological containment system designed to cause programmed bacterial cell lysis with no survivors. We have validated this system, using Salmonella enterica serovar Typhimurium vaccines for antigen delivery after colonization of host lymphoid tissues. The system is c...
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PMID: 18607005
PDF is available here.
Abstract
We examined the available evidence on the effectiveness of measures aimed at protecting humans and the environment against the risks of working with genetically modified microorganisms (GMOs) and with non-GMO pathogenic microorganisms. A few principles and methods underlie the current biosafety prac...
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PMID: 18625678
PDF is available here.
Abstract
We have reviewed existing cross-fertilization studies in maize, established relevant criteria for the evaluation of these studies and applied these criteria to define science-based isolation distances. To keep GM-inputs in the final product well below the 0.9% threshold defined by the EU, isolation...
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PMID: 17562214
PDF is available here.
Abstract
In 2004, a scientist from the US Army Medical Research Institute of Infectious Diseases (USAMRIID) was potentially exposed to a mouse-adapted variant of the Zaire species of Ebola virus. The circumstances surrounding the case are presented, in addition to an update on historical admissions to the me...
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PMID: 18507897
PDF is available here.
Abstract
The pursuit of scientific knowledge has been considerably advanced by the use of a wide variety of hazardous substances, including radioisotopes, hazardous chemicals, and biohazards. This appendix addresses each of these classes of hazards and primary safety precautions that need to be considered.
C...
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PMID: 18428418
PDF is available here.
Abstract
With the development of biological technology, many genetically engineered microorganisms (GEMs) for special purposes have been constructed and developed, but their practical applications in the field are still limited because GEMs may cause new environmental contaminations. To minimize the potentia...
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PMID: 18589808
PDF is available here.
Abstract
Use of genetically engineered microorganisms (GEMs) for pollution abatement has been limited because of the risks associated with their uncontrolled release in environment. In this study, a pollutant-dependent bacterial containment system was constructed in E. coli JM109. The system consisted of two...
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PMID: 18464620
PDF is available here.
Abstract
Even the best technical and organizational methods will not prevent the infection of the spot being operated on. The risks may be caused for example by the microbial air pollution of the operating theatre. Ways of air decontamination for the purposes of air conditioning and ventilating used in the o...
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PMID: 18669096
PDF is available here.
Abstract
The present paper reviews the current status of opinion and debate regarding ethical issues in three broad categories of relevance to animal biotechnology. The first is scientific integrity, where the focus has been on scientific fraud and the integrity of the research process. The second concerns p...
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PMID: 18154700
PDF is available here.
Abstract
Research over the past two decades has increased the quality and quantity of tools available to produce genetically engineered animals. The number of potentially viable biomedical products from genetically engineered animals is increasing. However, moving from cutting-edge research to development an...
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PMID: 18154699
PDF is available here.
Abstract
We propose that ecological risk evaluation and prevention strategies are indispensable important components and should be accompanied with breeding research in order to provide enlightments for transgenic fish breeding, evaluation of the ecological risks posed by transgenic fish, and development of...
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PMID: 17879053
PDF is available here.
Abstract
The development and implementation of an effective national biosafety system is important for several key reasons: to ensure safe access to products of modern biotechnology, to build public confidence, to encourage the growth of domestic modern biotechnology, and to comply with international standar...
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PMID: 17703486
PDF is available here.
Abstract
We illustrate a case study of seismic risk-benefit analysis for the city of Taipei, Taiwan, using available land use maps and surveys as well as a new tool developed by the National Science Council in Taiwan--the HAZ-Taiwan earthquake loss estimation system. We use three hypothetical earthquakes to...
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PMID: 17714167
PDF is available here.
Abstract
To investigate effective methods to sterilize a biological safety cabinet contaminated with Bacillus spores, a sterilization study was performed using the vaporized KMT reagent. Five kinds of test carriers inoculated with Bacillus subtilis spores, which had been prepared in-house, were used as indic...
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PMID: 17629248
PDF is available here.
Abstract
The threat of biocontamination in an animal facility is best subdued by training. 'Training' is an ambiguous designation that may not be adequately appreciated in all animal facilities. The authors set down concrete training topics and provide practical advice on incorporating the basic principles o...
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PMID: 17519943
PDF is available here.
Abstract
We report that plastids from the transplastomic father were transmitted in both cases at low (10(-4)-10(-5)) frequencies; therefore, rare paternal pollen transmission is not simply due to breakdown of normal controls caused by the alien cytoplasm. Furthermore, we have found that the entire plastid g...
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PMID: 17420457
PDF is available here.
Abstract
We have assessed the strictness of maternal inheritance and the extent to which plastid transformation technology confers an increase in transgene confinement. We describe an experimental system facilitating stringent selection for occasional paternal plastid transmission. In a large screen, we dete...
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PMID: 17420459
PDF is available here.
Abstract
In order to speculate the physical mechanisms of contaminant leakage during sash movement and walk-bys through a laboratory fume cupboard, the complicated three-dimensional flow patterns and the real-time tracer gas leakage are studied via the laser-assisted flow visualization method and the standar...
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PMID: 17485863
PDF is available here.
Abstract
Glutaraldehyde is a potential sensitizer and has been implicated in the literature as a cause of respiratory irritation and asthma among health care workers. In order to evaluate the effect of work practices and general ventilation system on employees' peak exposure to glutaraldehyde, 42 breathing z...
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PMID: 17485873
PDF is available here.
Abstract
Exploration Class missions to Mars will require precautions against potential contamination by any native microorganisms that may be incidentally pathogenic to humans. While the results of NASA's Viking biology experiments of the 1970s have been generally interpreted as inconclusive for surface orga...
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PMID: 17511302
PDF is available here.