Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Genetic Engineering (6):
Recent article
Genetic Engineering
Revue medicale suisse (7)312 2011
Abstract
| < More recent | Older article > |
Molecular Cell (43)6 2011
Abstract
RNA molecules play diverse functional roles in natural biological systems. There has been growing interest in designing synthetic RNA counterparts for programming biological function. The design of synthetic RNA molecules that exhibit diverse activities, including sensing, regulatory...
|
PMID: 21925380
PDF is available here.
PDF is available here.
Scientific American (305)2 2011
Abstract
Abstract
The Australian cotton industry has been a leader in the adoption of farm health and safety approaches in the Australian agricultural sector. In order to analyze factors associated with occupational health and safety performance, a review of available information and key stakeholder interviews were co...
|
PMID: 21919318
PDF is available here.
PDF is available here.
Abstract
We developed chimeric vaccine candidates using the non-structural protein genes of either Venezuelan equine encephalitis virus (VEEV) attenuated vaccine strain TC-83 or a naturally attenuated strain of eastern equine encephalitis virus (EEEV) and the structural genes of CHIKV. Because the transmissi...
|
PMID: 21633043
PDF is available here.
PDF is available here.
Abstract
A new reduced hydroxamate, 2,3-dihydrotrichostatin A, was created from trichostatin A by employing a recombinant strain of Streptomyces venezuelae as a microbial catalyst. Compared with trichostatin A, 2,3-dihydrotrichostatin A showed similar antifungal activity against Saccharomyces cerevisiae, but,...
|
PMID: 21504214
PDF is available here.
PDF is available here.
Nature Protocols (6)6 2011
Abstract
We describe here a detailed protocol for generating gene knockout rats by homologous recombination in embryonic stem (ES) cells. This protocol comprises the following procedures: derivation and expansion of rat ES cells, construction of gene-targeting vectors, generation of gene-targeted rat ES cell...
|
PMID: 21637202
PDF is available here.
PDF is available here.
Abstract
We report what are to our knowledge the first direct biocatalytic routes to BDO from renewable carbohydrate feedstocks, leading to a strain of Escherichia coli capable of producing 18 g l(-1) of this highly reduced, non-natural chemical. A pathway-identification algorithm elucidated multiple pathway...
|
PMID: 21602812
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2011)5 2011
Abstract
Engineering effective vectors has been crucial to the efficient delivery and expression of therapeutic gene products in vivo. Among these, HSV-1 represents an excellent candidate vector for delivery to the peripheral and central nervous systems. The natural biology of HSV-1 includes the establishmen...
|
PMID: 21536761
PDF is available here.
PDF is available here.
Molecular BioSystems (7)4 2011
Abstract
We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replicatio...
|
PMID: 21327279
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1807)7 2011
Abstract
The 6xHis-tag-pscA gene, which was genetically engineered to express N-terminally histidine (His)-tagged PscA, was inserted into a coding region of the recA gene in the green sulfur bacterium Chlorobaculum tepidum (C. tepidum). Although the inactivation of the recA gene strongly suppressed a homologo...
|
PMID: 21420930
PDF is available here.
PDF is available here.
Abstract
We propose a new inference algorithm that incorporates mutual information (MI), conditional mutual information (CMI), and predictive minimum description length (PMDL) principle to infer gene regulatory networks from DNA microarray data. In this algorithm, the information theoretic quantities MI and...
|
PMID: 21431544
PDF is available here.
PDF is available here.
Abstract
These results suggest that mmCTLA4 is a negative regulator of T cell activation and that mmCTLA4Ig may be useful for immunotherapy of immunologic diseases in the rhesus monkey....
|
PMID: 21349284
PDF is available here.
PDF is available here.
Abstract
Clostridium acetobutylicum is an anaerobic bacterium which is known for its solvent-producing capabilities, namely regarding the bulk chemicals acetone and butanol, the latter being a highly efficient biofuel. For butanol production by C. acetobutylicum to be optimized and exploited on an industrial...
|
PMID: 21247470
PDF is available here.
PDF is available here.
Abstract
We previously reported that in response to cytokinin-mediated HK activation in plants, the bacterial response regulator PhoB translocates to the nucleus and activates transcription. Also, we previously described a plant visual response system, the de-greening circuit, a threshold sensitive reporter...
|
PMID: 21283542
PDF is available here.
PDF is available here.
Abstract
We designed and constructed two recombinant influenza vaccine candidates by fusing hemagglutinin 1 (HA1) fragment of A/Anhui/1/2005(H5N1) to either Fc of human IgG (HA1-Fc) or foldon plus Fc (HA1-Fdc), and evaluated their immune responses and cross-protection against divergent strains of H5N1 virus....
|
PMID: 21304591
PDF is available here.
PDF is available here.
Abstract
We used cell lines genetically engineered for conditional asymmetric self-renewal, the defining DSC property. In gene microarray analyses, we identified 85 genes whose expression is tightly asymmetric self-renewal associated (ASRA). The ASRA gene signature prescribed DSCs to undergo asymmetric self-...
|
PMID: 21818293
PDF is available here.
PDF is available here.
Abstract
Abstract
We developed an engineered Sacchromyces cerevisiae strain to produce L-lactic acid efficiently by using glucose as carbon source.
|
PMID: 21465789
PDF is available here.
PDF is available here.
Voprosy Virusologii (56)1 2011
Abstract
Ebola virus virulence in guinea pigs, which appears through virus adaptation to this animal host, correlates with substitutions in the gene encoding vp24 protein. In particular, the substitution His-->Tyr186 was found when obtaining strain 8 ms. An attempt was made to clarify the functional role of t...
|
PMID: 21427954
PDF is available here.
PDF is available here.
Heart Rhythm (8)1 2011
Abstract
Abnormal conduction underlies both bradyarrhythmias and re-entrant tachyarrhythmias. However, no practical way exists for restoring or improving conduction in areas of conduction slowing or block.
This study sought to test the feasibility of a novel strategy for condu...
|
PMID: 20951232
PDF is available here.
PDF is available here.
Abstract
We provide a detailed protocol of the design of a mammalian cell-to-cell signaling device and its integration into a mammalian quorum-sensing system for cell density-induced expression product genes. Cell-to-cell signaling is based on a sender cell, metabolically engineered for expression of alcohol...
|
PMID: 21031316
PDF is available here.
PDF is available here.
Abstract
We have exploited the natural transformability and powerful homologous recombination capacity of this organism by using linear DNA fragments for transformation. This approach overcomes barriers that have made the introduction and expression of foreign genes problematic in the past. Foremost among th...
|
PMID: 20960136
PDF is available here.
PDF is available here.
Abstract
A series of protocols are presented for the storage, growth, transformation, and characterization of wild type and mutant strains of Synechocystis sp. strain PCC 6803. These protocols include the isolation of genomic DNA and the strategies required for the construction of specific gene interruptions...
|
PMID: 20960137
PDF is available here.
PDF is available here.
Abstract
I (PSI) is a multisubunit pigment-protein complex that uses light energy to transfer electrons from plastocyanin to ferredoxin. Application of genetic engineering to photosynthetic reaction center proteins has led to a significant advancement in our understanding of primary electron transfer events...
|
PMID: 20960138
PDF is available here.
PDF is available here.
Abstract
We describe sources of environmental chromium contamination, and provide information about the toxic impact of chromium on plant growth and metabolism. In addition, we address different phytoremediation processes that are being studied for use worldwide, in contaminated regions, to address and mitig...
|
PMID: 21170701
PDF is available here.
PDF is available here.
Abstract
Abstract
We have shown previously that immunoglobulin fragment-fused GLP-1 (GLP-1/Fc) is a structurally stable GLP-1 analog. Here, we report a non-viral GLP-1/Fc gene therapy strategy utilizing a REP78-in-trans and REB-in-cis element system to achieve a site-specific genomic integration. For this purpose, th...
|
PMID: 21070745
PDF is available here.
PDF is available here.
Abstract
We show that mice lacking the functional leptin receptor developed devastating mucosal destruction after E. histolytica infection. Bone marrow chimera experiments demonstrated that leptin receptor expressed on hematopoietic cells was not sufficient to confer resistance. Similarly, peripheral knockou...
|
PMID: 21124310
PDF is available here.
PDF is available here.
Abstract
The efficacy of mesenchymal stem cell (MSC) therapy for myocardial regeneration is limited by the poor survival of stem cells after transplantation into the infarcted heart. To improve the cell survival of MSCs in the infarcted heart, MSCs were genetically engineered to overexpress phosphoinositide-...
|
PMID: 20937252
PDF is available here.
PDF is available here.
Abstract
Abstract
Human Vaccines (6)11 2010
Abstract
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology (18)6 2010
Abstract
This study was aimed to construct a lentiviral vector of ha1 gene and obtain a stable packaging cell lines, which will be beneficial to guiding further study on the foundation of ha1 on the dissociation of graft versus host disease (GVHD) and graft versus leukemia (GVL) effect after allogenic hematop...
|
PMID: 21176380
PDF is available here.
PDF is available here.
Journal of Hepatology (54)6 2011
Abstract
We characterized 676 genes by this approach. Among these genes, 39 are novel direct targets of C/EBPbeta. Eleven of these new direct targets are involved in cell survival, suggesting critical roles for LAP/LIP isoforms in this cellular process. Therefore, we examined the effects of LAP and LIP over-...
|
PMID: 21145827
PDF is available here.
PDF is available here.
Journal of Plant Physiology (167)16 2010
Abstract
We described improved versions of MultiRound Gateway vectors. Here, we engineered a calcineurin B-like (CBL) pathway for potassium (K+) nutrition to demonstrate their effectiveness. Using the two improved entry vectors pL12R34H-Ap and pL34R12-Cm, and through 2-4 rounds of Gateway recombination react...
|
PMID: 20708297
PDF is available here.
PDF is available here.
Abstract
Innate immune recognition of the bacterial cell wall constituent peptidoglycan by the cytosolic nucleotide-binding oligomerization domain 2 (Nod2) receptor has a pivotal role in the maintenance of intestinal mucosal homeostasis. Whereas peptidoglycan cleavage by gut-derived lysozyme preserves the rec...
|
PMID: 20980996
PDF is available here.
PDF is available here.
Abstract
We developed a method to recover recombinant viruses in which independent selection strategies are used to engineer single-gene replacements. We coupled a mutant SA11 RV encoding a temperature-sensitive (ts) defect in the NSP2 protein with RNAi-mediated degradation of NSP2 mRNAs to isolate a virus c...
|
PMID: 20937889
PDF is available here.
PDF is available here.
Abstract
We have witnessed a biotech asset yard sale. Despite any monetary shortcomings, from a technological point of view there have been great leaps that are yet to find their way to the patient. RegMed is definitely bound to play a major role in our life because it embodies one of the primordial dreams o...
|
PMID: 20825521
PDF is available here.
PDF is available here.
Nature Biotechnology (28)10 2010
Abstract
Comparative analysis of epigenomes offers new opportunities to understand cellular differentiation, mutation effects and disease processes. But the scale and heterogeneity of epigenetic data present numerous computational challenges.
|
PMID: 20944597
PDF is available here.
PDF is available here.
Clinical Immunology (136)3 2010
Abstract
MHC class I-restricted human melanoma epitope MART-1(27-35) specific TCR-engineered CD4+CD25- T cells synthesize Th1 type cytokines and exhibit cytolytic effector function upon cognate stimulation. A detailed characterization of such TCR-engineered CD4+CD25- T cells now reveals that they are multifu...
|
PMID: 20547105
PDF is available here.
PDF is available here.
Immunotherapy (2)5 2010
Abstract
Asthma is a chronic inflammatory disorder of the airways in which many cells and cellular elements play a role. Chronic inflammation is associated with airway hyper-responsiveness that leads to recurrent episodes of wheezing, breathlessness, chest tightness and coughing, as well as v...
|
PMID: 20874654
PDF is available here.
PDF is available here.
Abstract
We expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of...
|
PMID: 20890094
PDF is available here.
PDF is available here.
Abstract
Ethyl (R, S)-4-chloro-3-hydroxybutanoate (ECHB) is a useful chiral building block for the synthesis of L-carnitine and hypercholesterolemia drugs. The yeast reductase, YOL151W (GenBank locus tag), exhibits an enantioselective reduction activity, converting ethyl-4-chlorooxobutanoate (ECOB) exclusive...
|
PMID: 20890095
PDF is available here.
PDF is available here.
Abstract
The Phi CTX-based integration vector pYM101 harboring a tightly controlled modified phage T7 early gene promoter/LacI(q) repressor (T7/LacI) system was constructed for the generation of unmarked conditional mutants in Pseudomonas aeruginosa. Promoter activity of the T7/LacI system was demonstrated t...
|
PMID: 20538017
PDF is available here.
PDF is available here.
Regulatory Peptides (163)1-3 2010
Abstract
We constructed a recombinant adenoviral vector Ad-leptin and transfected propagated MSCs in vitro. The effects of Ad-leptin on MSCs growth and osteogenic differentiation were examined. The results showed that Ad-leptin inhibited the transfected MSCs growth significantly, and up-regulated osteocalcin...
|
PMID: 20433876
PDF is available here.
PDF is available here.
Journal of Immunological Methods (359)1-2 2010
Abstract
We have setup and validated a T cell activation assay to test antigen expression on patient-derived tumor tissues. Chimeric antibody-based receptor (CAR) directed against CAIX, currently used in a clinical trial to treat RCC patients, was used as a model receptor. Primary human T cells expressing CA...
|
PMID: 20460126
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We have constructed a novel oncolytic HSV-1 recombinant, KTR27, whose replication can be tightly controlled and regulated by tetracycline in a dose-dependent manner. Infection of normal replicating cells as well as multiple human cancer cell types with KTR27 in the presence of tetracycline led to 1,...
|
PMID: 20519407
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)8 2010
Abstract
We successfully improved the production of both the enediyne antitumor antibiotic C-1027 and a heptaene, an early metabolite of the C-1027 pathway, by manipulating the three regulatory genes, sgcR1, sgcR2 and sgcR3, within the C-1027 biosynthetic gene cluster. SgcR3 has previously been established a...
|
PMID: 20551990
PDF is available here.
PDF is available here.
Abstract
Spiders and silkworms generate silk protein fibers that embody strength and beauty. Orb webs are fascinating feats of bioengineering in nature, displaying magnificent architectures while providing essential survival utility for spiders. The unusual combination of high strength and extensibility is a...
|
PMID: 20671180
PDF is available here.
PDF is available here.
Biotechnology Letters (32)8 2010
Abstract
To improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehyd...
|
PMID: 20354759
PDF is available here.
PDF is available here.
Abstract
Chilli fruit is highly susceptible to anthracnose infection at the stage of harvest maturity, due to which the fruit yield in the leading commercial variety Byadgi is severely affected. Field studies on screening of several varieties for resistance to anthracnose have shown that a variety of chilli...
|
PMID: 21174952
PDF is available here.
PDF is available here.
Abstract
Endoglucanase is a major cellulolytic enzyme produced by the fungus Trichoderma viride. The 1,317 bp cDNA of endoglucanase gene egVIII was cloned from T. viride AS3.3711, encoding a 438 amino acid protein with a calculated molecular mass of 46.86 kDa and isoelectric point of 4.32. Sequence analysis...
|
PMID: 19590984
PDF is available here.
PDF is available here.
Abstract
Plants have evolved a variety of gene silencing pathways mediated by small RNAs. Mostly 21 or 24 nt in size, these small RNAs repress the expression of sequence homologous genes at the transcriptional, post-transcriptional and translational levels. These pathways, also referred as RNA silencing path...
|
PMID: 20331529
PDF is available here.
PDF is available here.
Abstract
We identify features of RNAa and explore chemical modifications to saRNAs that improve the applicability of RNAa. We evaluate the rate of RNAa activity in order to define an optimal window of gene induction, while comparing the kinetic differences between RNAa and RNAi. We identify Ago2 as a conserv...
|
PMID: 20662764
PDF is available here.
PDF is available here.
Abstract
We sought to assess the versatility of transgenic algae as a recombinant protein production platform. To do this, we tested whether the algal chloroplast could support the expression of a diverse set of current or potential human therapeutic proteins. Of the seven proteins chosen, >50% expressed at...
|
PMID: 20230484
PDF is available here.
PDF is available here.
Abstract
We conclude by discussing not only the current limitations but also possible ways to transform the construction of synthetic mammalian systems from an art into a predictive engineering discipline.
Copyright © 2010 Elsevier Ltd. All rights reserved....
|
PMID: 20691580
PDF is available here.
PDF is available here.
Abstract
We describe the testing of these vectors through marker gene expression in Coprinopsis cinerea. This work also extends the range of marker genes available for use in C. cinerea with the first report of DsRed and monomeric red fluorescent protein (mRFP) expression in C. cinerea and further demonstrat...
|
PMID: 20570599
PDF is available here.
PDF is available here.
Abstract
We report here a versatile gene expression method for the genetic engineering of R. eutropha. This method, based on a simplified electroporation protocol, uses a recombinant plasmid, pBS29-P2, containing a Pseudomonas syringae promoter (P2) and two antibiotic-resistance markers (i.e., genes coding f...
|
PMID: 20447426
PDF is available here.
PDF is available here.