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In Situ Hybridization (2):
Recent article
In Situ Hybridization
Abstract
We focus on the planarian excretory system, which consists of internal protonephridial tubules. We find that these assemble into complex branching patterns with a stereotyped succession of cell types along their length. Organ regeneration is likely to originate from a precursor structure arising in...
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PMID: 21828097
PDF is available here.
PDF is available here.
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Abstract
We analyzed the function of the Delta (Gb'Delta) and Notch (Gb'Notch) genes in the hemimetabolous, intermediate-germ cricket Gryllus bimaculatus. We found that Gb'Delta and Gb'Notch were expressed in developing legs, and that RNAi silencing of Gb'Notch resulted in a marked reduction in leg length wi...
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PMID: 21828099
PDF is available here.
PDF is available here.
Abstract
We isolated a novel Drosophila male sterile mutant, garmcho (garm). Positional cloning indicated that garm encodes a subunit of the Drosophila THO complex, THOC5. Flies lacking THOC5 showed a meiotic arrest phenotype with severe nucleolar disruption in primary spermatocytes. A functional GFP-tagged...
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PMID: 21828100
PDF is available here.
PDF is available here.
Abstract
Interlaboratory variation in human epidermal growth factor receptor 2 (HER2) testing provides a challenge for targeted therapy in breast and gastric cancer. Assessment of positivity rates among laboratories could help monitor their performance and define reference values for positivity rates to be ex...
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PMID: 21809092
PDF is available here.
PDF is available here.
Abstract
We therefore report the development of a markedly improved approach combining fluorescence-based miRNA-in situ hybridization (miRNA-ISH) with immunohistochemistry (IHC). We have applied this protocol to differentiating embryoid bodies (EBs) as well as embryonic and adult mouse hearts, to detect miRN...
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PMID: 21643937
PDF is available here.
PDF is available here.
Abstract
Abstract
We transduced SATB2 into murine adult stem cells, and found that SATB2 significantly increased expression levels of bone matrix proteins, osteogenic transcription factors, and a potent angiogenic factor, vascular endothelial growth factor. Using an osterix (Osx) promoter-luciferase construct and cal...
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PMID: 21385070
PDF is available here.
PDF is available here.
Abstract
Hypermethylation of tumor suppressor genes is significantly more frequent in EBV-associated GC compared to EBV-negative GC. Our data add new insights to the role of EBV in gastric carcinogenesis and underline that EBV associated GC comprise a distinct molecular-pathologic as well as a distinct clini...
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PMID: 21538028
PDF is available here.
PDF is available here.
Abstract
We show that loss-of-function mutations in SPECC1L are pathogenic for this human developmental disorder and that SPECC1L is a critical organizer of vertebrate facial morphogenesis. During murine embryogenesis, Specc1l is expressed in cell populations of the developing facial primordial, which pr...
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PMID: 21703590
PDF is available here.
PDF is available here.
Abstract
A well-balanced immunological interaction between mother and the semi-allogenic embryo is of particular importance. The objective of the present study was to analyse mechanisms of immune tolerance in bovine pregnancy during peri-implantation. Simmental heifers inseminated with either cryopreserved sp...
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PMID: 21635810
PDF is available here.
PDF is available here.
Abstract
We report for the first time the use of DNA hybridization for the controllable assembly of a graphene nanosheet. Moreover, with the help of dynamic light scattering technique, we extended the above studies by exploiting the DNA-graphene dispersed sheets as highly ultrasensitive detection of oligonul...
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PMID: 21534537
PDF is available here.
PDF is available here.
Gene profiling of the rat medial collateral ligament during early healing using microarray analysis.
Abstract
We demonstrate that early ligament healing is characterized by the modulation of several inflammatory and extracellular matrix factors during the first week of injury. Specifically, a number of matrix metalloproteinases and collagens are differentially and significantly expressed during early ligame...
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PMID: 21596919
PDF is available here.
PDF is available here.
Abstract
We used in vivo BrdU labeling and markers specific to stages of macrophage differentiation or inflammation to define macrophage heterogeneity and to better define the role of macrophage populations in lesion formation and productive infection. Lesions were heterogeneously composed of resident macrop...
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PMID: 21514427
PDF is available here.
PDF is available here.
Development (138)8 2011
Abstract
We generated three mouse lines, each of which lacks one out of three prominent PCDH15 isoforms (CD1, CD2 and CD3). Surprisingly, mice lacking PCDH15-CD1 and PCDH15-CD3 form normal hair bundles and tip links and maintain hearing function. Tip links are also present in mice lacking PCDH15-CD2. However...
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PMID: 21427143
PDF is available here.
PDF is available here.
Abstract
We determined that CYP85A1 is abundantly expressed in wild-type but not in sporocyteless (spl) ovules lacking a female gametophyte. Insertional T-DNA lines defective in the activity of CYP85A1 exhibit a semi-sterile phenotype, suggesting a role for the corresponding enzyme acting at the gametophytic...
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PMID: 21364326
PDF is available here.
PDF is available here.
Abstract
We demonstrate the feasibility of this method in Drosophila melanogaster, utilizing the well-characterized HEG, I-SceI. In particular, we show that high rates of homing can be achieved within spermatogonia and in the female germline. We show that homed constructs continue to exhibit HEG activity in...
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PMID: 21368273
PDF is available here.
PDF is available here.
Abstract
Oncogenic human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (SCC) is a subtype of head-and-neck cancer with a distinct clinical and prognostic profile. While there are calls to undertake HPV testing for oropharyngeal SCCs within the diagnostic setting and for clinical trials...
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PMID: 21345874
PDF is available here.
PDF is available here.
Abstract
To evaluate the role of the follicular helper T (T(FH)) cell markers, CD10, BCL6, programmed death-1 (PD-1) and CXCL13, in the differential diagnosis of nodal peripheral T cell lymphomas (PTCLs) and to determine whether PTCL subtypes other than angioimmunoblastic T cell lymphoma (AITL) express T(FH)...
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PMID: 21330314
PDF is available here.
PDF is available here.
Abstract
We analyze the role of Schmidtea mediterranea Dvl homologs (Smed-dvl-1 and Smed-dvl-2) using gene silencing. We demonstrate that in addition to a role in AP axis specification, planarian Dvls are involved in at least two different β-catenin-independent processes. First, they are essential for neura...
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PMID: 21282632
PDF is available here.
PDF is available here.
Abstract
We are still missing the batteries of genes targeted by any one Hox gene over the course of a single developmental process to achieve a particular cell and organ morphology. The transformation of wings into halteres by the Hox gene Ultrabithorax (Ubx) in Drosophila melanogaster presents an excellent...
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PMID: 21282633
PDF is available here.
PDF is available here.
Abstract
We examined ZAC/Zac1 expression in developing mouse and human pancreas by real-time PCR and dual in situ hybridization and immunofluorescence. Overall pancreatic expression drastically declined during gestation and early post-natal life in the mouse, and between the second trimester and adult in the...
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PMID: 21305342
PDF is available here.
PDF is available here.
Abstract
We specifically deleted Fbxw7 in the murine gut using Villin-Cre (Fbxw7(ΔG)). In wild-type mice, loss of Fbxw7 in the gut altered homeostasis of the intestinal epithelium, resulted in elevated Notch and c-Jun expression, and induced development of adenomas at 9-10 mo of age. In the context of APC (...
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PMID: 21282377
PDF is available here.
PDF is available here.
Abstract
We have discovered that pretransplant conditioning regimen damaged ISCs; however, the ISCs rapidly recovered and restored the normal architecture of the intestine. ISCs are targets of GVHD, and this process of ISC recovery was markedly inhibited with the development of GVHD. Injection of Wnt agonist...
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PMID: 21282378
PDF is available here.
PDF is available here.
Abstract
We analyzed the expression of C1q, the initial recognition subcomponent of the classic complement cascade, in the substantia nigra pars compacta (SNc) in Parkinson disease (PD) and control cases using immunohistochemistry and in situ hybridization. Microglia were determined to be the only cells that...
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PMID: 21343881
PDF is available here.
PDF is available here.
Abstract
We show that mutations in prickle genes are associated with seizures in humans, mice, and flies. We identified human epilepsy patients with heterozygous mutations in either PRICKLE1 or PRICKLE2. In overexpression assays in zebrafish, prickle mutations resulted in aberrant prickle function. A seizure...
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PMID: 21276947
PDF is available here.
PDF is available here.
Abstract
We detected linkage of nonsyndromic hearing loss to a 7.6 Mb region on chromosome 3q13.31-q21.1 within the previously reported DFNB42 locus. Subsequent candidate gene sequencing identified a homozygous nonsense mutation (c.1135G>T [p.Glu379X]) in ILDR1 as the cause of hearing impairment. By analyzin...
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PMID: 21255762
PDF is available here.
PDF is available here.
Abstract
We examined in rodents (voles and rat) the conservation of the microsatellite region DXPas34, the Tsix gene (antisense counterpart of Xist), and enhancer Xite that have been shown to flank Xist and regulate XCI in mouse. We have found that mouse regions of the Tsix gene major promoter and minisatell...
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PMID: 21826206
PDF is available here.
PDF is available here.
Abstract
We investigate the hypothesis that micro RNAs regulate the ability of the early telencephalic progenitors to establish radial glia. We ablate functional Dicer, which is required for the generation of mature micro RNAs, by conditionally mutating the Dicer1 gene in the early embryonic telencephalon an...
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PMID: 21826226
PDF is available here.
PDF is available here.
Abstract
The results suggest that translocations can be used as a specific test to identify individuals with a potentially elevated radiosensitivity....
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PMID: 21473753
PDF is available here.
PDF is available here.
Abstract
We use optical projection tomography to provide a detailed three-dimensional and quantitative description of pancreatic growth dynamics in the mouse. Hereby, we describe the epithelial and mesenchymal events leading to the formation of the gastric lobe of the pancreas. We show that this structure fo...
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PMID: 21738788
PDF is available here.
PDF is available here.
Abstract
We evaluated the alien introgressions PW11-2, PW11-5 and PW11-8 which were derived from line PW11 for reaction to new Pst race CYR32, and used molecular and cytogenetic tools to characterize these lines. The introgressions were remarkably resistant to CYR32, suggesting that the resistance to stripe...
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PMID: 21760909
PDF is available here.
PDF is available here.
Abstract
In limb regeneration of amphibians, the early steps leading to blastema formation are critical for the success of regeneration, and the initiation of regeneration in an adult limb requires the presence of nerves. Xenopus laevis tadpoles can completely regenerate an amputated limb at the early limb bu...
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PMID: 21814549
PDF is available here.
PDF is available here.
Abstract
We analyzed the function of two BMP downstream transcription factors, Msx2 and Foxn1, in nail differentiation. First we show that Msx2 function is required during onychocyte (nail cell) terminal differentiation. Second, the Msx2/Foxn1/hair keratin pathway controlling hair differentiation is also con...
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PMID: 21387539
PDF is available here.
PDF is available here.
Abstract
Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development.
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PMID: 21829441
PDF is available here.
PDF is available here.
Abstract
We present a thorough qualitative and quantitative analysis of nematocysts in Nematostella vectensis. In addition, we have cloned major nematocyst components, Nematostella minicollagens 1, 3 and 4, and show their expression patterns by in situ hybridization and immunocytochemistry using specific ant...
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PMID: 21829492
PDF is available here.
PDF is available here.
Abstract
We hypothesized that 3',4'-dihydroxyflavonol (DiOHF), a small highly lipid soluble synthetic flavonol, may prevent DCM by scavenging ROS, thus preventing ROS-induced cardiac damage....
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PMID: 21829510
PDF is available here.
PDF is available here.
Abstract
Presently we investigated the function of periostin in cutaneous wound healing by using periostin-deficient (-/-) mice. Periostin mRNA was expressed in both the epidermis and hair follicles, and periostin protein was located at the basement membrane in the hair follicles together with fibronectin an...
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PMID: 21490918
PDF is available here.
PDF is available here.
Abstract
We identified the chicken chorioallantoic membrane (CAM) as a model system to study the endogenous function of TEM8 in blood vessels as we found that TEM8 expression was induced transiently between day 10 and 12 of embryonic development, when the vascular tree is undergoing final development and gro...
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PMID: 21829615
PDF is available here.
PDF is available here.
Abstract
We speculate that intrastrain transposition of the three retroelements studied is controlled predominantly at the post-transcriptional level....
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PMID: 21779346
PDF is available here.
PDF is available here.
Neural Development (6)1 2011
Abstract
We studied the temporal generation and diversity of Neurog2-positive precursor progeny using an inducible genetic fate mapping approach. We show that all subtypes of glutamatergic neurons derive from Neurog2 positive progenitors during development of the OB. Projection neurons, that is, mitral and t...
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PMID: 21466690
PDF is available here.
PDF is available here.
Gene Expression Patterns (11)1-2 2011
Abstract
We used in situ hybridization to screen numerous expression patterns of the TFs involved in the development of the mouse thymus. More than 400 members, including unique TFs and some transcription co-factors, were tested. Among the screened TFs, 160 were found to be expressed in the thymus after E15....
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PMID: 20932939
PDF is available here.
PDF is available here.
Sexual Development (5)2 2011
Abstract
The process of morphological development of a differentiated gonad from an undifferentiated primordium is a very important step of gonadogenesis. Studies on sexually dimorphic gene expression are important to increase our understanding of this process and to investigate how environme...
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PMID: 21325793
PDF is available here.
PDF is available here.
Abstract
Histological procedures aim to provide good quality sections that can be used for a light microscopic evaluation of human or animal tissue changes in either spontaneous or induced diseases. Routinely, tissues are fixed with neutral formalin 10%, embedded in paraffin, and then manually sectioned with...
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PMID: 20972747
PDF is available here.
PDF is available here.
Molecular Carcinogenesis (50)1 2011
Abstract
We examined Prr15 expression by in situ hybridization (ISH) on mouse intestinal tumors caused by different gene mutations, and on human colorectal cancer (CRC) samples. Prr15/PRR15 expression was consistently observed in mouse gastrointestinal (GI) tumors caused by mutations in the Apc gene, as well...
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PMID: 21061267
PDF is available here.
PDF is available here.
Abstract
Extracorporeal photochemotherapy (ECP) is widely used to treat cutaneous T-cell lymphoma, graft-versus-host disease, and allografted organ rejection. Its clinical and experimental efficacy in cancer immunotherapy and autoreactive disorders suggests a novel mechanism. This study reveals that ECP indu...
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PMID: 20720185
PDF is available here.
PDF is available here.
Clinical Cancer Research (17)6 2011
Abstract
High-motility group AT-hook gene 1 (HMGA1) is a non-histone nuclear binding protein that is developmentally regulated. HMGA1 is significantly overexpressed in and associated with high grade and advance stage of prostate cancer (PC). The oncogenic role of HMGA1 is at least mediated through chromosomal...
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PMID: 21138859
PDF is available here.
PDF is available here.
Abstract
We profiled luminal A, ESR1+ breast cancer cell lines versus triple negative (TN), which lack ERα, progesterone receptor and Her2/neu. Although two thirds of the differentially expressed miRNAs are higher in ESR1+ breast cancer cells, some miRNAs, such as miR-222/221 and miR-29a, are dramatically h...
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PMID: 21761362
PDF is available here.
PDF is available here.
Abstract
The human epidermal growth factor receptor-2 gene (HER-2) encodes for a membrane-bound tyrosine kinase (Her-2), which is overexpressed in various human cancers. Her-2-targeted therapy has recently been shown to be beneficial for patients with advanced gastric cancer. Her-2 protein expression was inv...
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PMID: 21107094
PDF is available here.
PDF is available here.
Abstract
We have reviewed clinically, morphologically, and immunophenotypically a series of 14 Epstein-Bar virus (EBV)+ cutaneous natural killer cell (NK)/T-cell lymphoma from Peru. Most (11 out of 14) of these cases fit well into the category of Hydroa vacciniforme-like lymphoma (HVLL), but 3 have a differe...
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PMID: 21107082
PDF is available here.
PDF is available here.
Abstract
A novel member of the human ppGalNAc-T family, ppGalNAc-T20, was identified and characterized. Amino acid alignment revealed a high sequence identity between ppGalNAc-T20 and -T10. In the GalNAc transfer assay towards mucin-derived peptide substrates, the recombinant ppGalNAc-T20 demonstrated to be...
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PMID: 20977886
PDF is available here.
PDF is available here.
Bulletin du cancer (97)12 2010
Abstract
HER2 protein overexpression by immunohistochemistry (IHC) and/or erB2 gene amplification by in situ hybridization (ISH) was detected in 4-28% of gastric or gastro-oesophageal junction (GOJ) cancers. Most studies have shown that HER2-overexpressing gastric cancers were worse prognosis. Trastuzumab is...
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PMID: 21134821
PDF is available here.
PDF is available here.
Abstract
Blood intake causes significant changes in ticks, triggering vital physiological processes including differential gene expression. A gene encoding Ixodes ricinus ML-domain containing protein (IrML) is one of the set of the genes that are strongly induced by blood meals. IrML belongs to the ML protein...
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PMID: 21175949
PDF is available here.
PDF is available here.
Nature Medicine (16)12 2010
Abstract
We searched for GLI1-interacting proteins. We report that the chromatin remodeling protein SNF5 (encoded by SMARCB1, hereafter called SNF5), which is inactivated in human malignant rhabdoid tumors (MRTs), interacts with GLI1. We show that Snf5 localizes to Gli1-regulated promoters and that loss of S...
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PMID: 21076395
PDF is available here.
PDF is available here.
Abstract
We report the case of a middle age HIV male, presenting with intestinal occlusion. Rectosigmoidoscopy showed multiple anorectal nodular and ulceronecrotic masses. The biopsy specimens revealed a diffuse polymorphous inflammatory infiltrate in the lamina propria, associated with CD30, CD20, CD3, CD15...
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PMID: 20861713
PDF is available here.
PDF is available here.
Cancer Research (70)21 2010
Abstract
We transformed rat 9L gliosarcoma cells to express cel-miR-67, a miRNA that lacks homology in rat. Coculture of these cells with cells that expressed a luciferase reporter that contained a complementary sequence to cel-miR-67 resulted in significant suppression of luciferase expression. This effect...
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PMID: 20841486
PDF is available here.
PDF is available here.
Abstract
Squamous cell carcinomas of the head and neck (HNSCC) are a frequent diagnosis in anatomic pathology practice. Tobacco use and heavy alcohol consumption are known risk factors for HNSCC but in other cases human papillomavirus (HPV) is linked to carcinogenesis. HPV proteins E6 and E7 promote oncogene...
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PMID: 20966645
PDF is available here.
PDF is available here.
EMBO Journal (29)21 2010
Abstract
We show that zebrafish hen1 is specifically expressed in germ cells and is essential for maintaining a female germ line, whereas it is dispensable in the testis. Hen1 protein localizes to nuage through its C-terminal domain, but is not required for nuage formation. In hen1 mutant testes, piRNAs beco...
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PMID: 20859253
PDF is available here.
PDF is available here.
Abstract
We reviewed clinical, morphologic, and immunophenotypic features of 13 cases of PBL to determine whether these different subtypes represent distinct morphologic and clinical entities. Two distinct subtypes of PBL were identified and classified as oral and extraoral PBL. The oral PBLs were strongly a...
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PMID: 20959653
PDF is available here.
PDF is available here.
Abstract
We have investigated a gene called nematogalectin in Hydra, which has a structural role in the formation of nematocysts, stinging organelles that are unique to the phylum Cnidaria. Nematogalectin is a 28-kDa protein with an N-terminal GlyXY domain (glycine followed by two hydrophobic amino acids), w...
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PMID: 20937891
PDF is available here.
PDF is available here.
Abstract
We examined sleep and wakefulness in mice deficient in a homeobox transcription factor, Goosecoid-like (Gscl), which is one of the genes deleted in DiGeorge syndrome or 22q11 deletion syndrome. The expression of Gscl is restricted to the interpeduncular nucleus (IP) in the ventral region of the midb...
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PMID: 20921407
PDF is available here.
PDF is available here.