Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Nucleic Acid Hybridization (5):
Recent article
Nucleic Acid Hybridization
Abstract
We present six design rules that can be used to deliberately prepare nine distinct colloidal crystal structures, with control over lattice parameters on the 25- to 150-nanometer length scale. These design rules outline a strategy to independently adjust each of the relevant crystallographic paramete...
|
PMID: 21998382
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Abstract
To evaluate GenoType MTBDRplus (Hain Lifescience, Germany) for its capacity to detect the resistance of rifampicin (RFP) and isoniazid (INH).
|
PMID: 21922778
PDF is available here.
PDF is available here.
Abstract
Several of the more recently proposed new species of Enterococcus are nearly identical based on 16S rRNA gene sequence analysis and phenotypic traits. In the present study, DNA-DNA reassociation experiments, in conjunction with sequencing of the 16S rRNA and rpoB genes, provided evidence that "Entero...
|
PMID: 21543565
PDF is available here.
PDF is available here.
Abstract
We developed a bexB-based method to differentiate true NTHI strains from typeable strains. We validated a PCR-based method to detect bexB in 10 strains whose capsule status was well defined. Among 40 strains that were previously serotype positive in clinical microbiology laboratories, 5 lacked bexA,...
|
PMID: 21525217
PDF is available here.
PDF is available here.
Abstract
Because almost all implant systems reveal marginal bone loss and some implants can be affected by periimplantitis, to realize an effective maintenance treatment it is important to understand the biofilm development over different implant surfaces. The objective of this human study is to comparatively...
|
PMID: 21448015
PDF is available here.
PDF is available here.
Abstract
We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands...
|
PMID: 21388119
PDF is available here.
PDF is available here.
Abstract
Our approach is a significant step forward for the development of a point of care test for CT....
|
PMID: 20709634
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (27)2 2011
Abstract
Abstract
Many biomolecules have specific binding properties in the nanostructure formation; they are attractive materials for nanotechnology. One such promising construction material for growing a well-defined nanostructure is deoxyribonucleic acid, due to its π-electron hydrophobic core and predictable reco...
|
PMID: 21382609
PDF is available here.
PDF is available here.
Abstract
The resource authentication is required for quality assurance and control of Chinese medicine. This review provides an informative introduction to molecular methods used for authentication of Chinese medicinal materials. The technical features of the methods based on sequencing, polymerase chain reac...
|
PMID: 21585017
PDF is available here.
PDF is available here.
Sexual Health (8)1 2011
Abstract
Sexually transmissible infections (STIs) remain highly prevalent, and HIV is increasing, among female sex workers (FSWs) in Indonesia. Our aim was to determine the prevalence of, and risk factors for, STIs among FSWs in Manado, Indonesia.
|
PMID: 21371382
PDF is available here.
PDF is available here.
Abstract
We analyzed hybridizations of natural accessions to the Arabidopsis ATSNPTILE array and simulated BSA or XAM given a variety of gene models, populations, and bulk selection parameters. Our results show a striking degree of correlation between the genotyping output of both methods, which suggests tha...
|
PMID: 21297997
PDF is available here.
PDF is available here.
Abstract
We propose a novel method for sRNA target prediction, termed sTarPicker, which was based on a two-step model for hybridization between an sRNA and an mRNA target. This method first selects stable duplexes after screening all possible duplexes between the sRNA and the potential mRNA target. Next, hyb...
|
PMID: 21799937
PDF is available here.
PDF is available here.
Abstract
While it is established that vertebrate-like steroids, particularly estrogens (estradiol, estrone) and androgens (testosterone), are present in various tissues of molluscs, it is still unclear what role these play in reproductive endocrinology in such organisms. This is despite the significant commer...
|
PMID: 21818309
PDF is available here.
PDF is available here.
Abstract
We have developed a set of doubly-fluorophore labeled RNAs (more precisely, RNA/DNA chimeras) to probe in real-time the stability of siRNAs and related molecules by fluorescence resonance energy transfer (FRET). We find that these RNA probes are substrates for relevant cellular degradative processes...
|
PMID: 21647381
PDF is available here.
PDF is available here.
Abstract
We have recently demonstrated that the T. brucei telomere structure plays an essential role in regulation of surface antigen gene expression, which is critical for T. brucei pathogenesis. However, T. brucei telomere structure has not been extensively studied due to the limitation of methods for anal...
|
PMID: 21307825
PDF is available here.
PDF is available here.
Abstract
We aimed to identify novel sRNAs and sORFs in Shigella, the principal etiologic agents of bacillary dysentery.
We identified 64 sRNAs in Shigella, which were experimentally validated in other bacteria based on sequence conservation. We employed computer-based and tiling array-based m...
|
PMID: 21483688
PDF is available here.
PDF is available here.
Abstract
We describe the use of one method commonly used to generate transcriptomics data, namely the use of Affymetrix GeneChip microarrays. Data generated in transcriptomics experiments can be analysed using a multitude of approaches, but a common goal is to identify those transcripts whose abundance is al...
|
PMID: 20972753
PDF is available here.
PDF is available here.
Abstract
We investigated the effects of exposure to RF fields (2.45 GHz, continuous wave) at specific absorption rate (SAR) of 1, 5, and 10 W/kg for 1, 4, and 24 h on gene expression in a normal human glial cell line, SVGp12, using DNA microarray. Microarray analysis revealed 23 assigned gene spots and 5 non...
|
PMID: 21343680
PDF is available here.
PDF is available here.
Molecular Carcinogenesis (50)1 2011
Abstract
We examined Prr15 expression by in situ hybridization (ISH) on mouse intestinal tumors caused by different gene mutations, and on human colorectal cancer (CRC) samples. Prr15/PRR15 expression was consistently observed in mouse gastrointestinal (GI) tumors caused by mutations in the Apc gene, as well...
|
PMID: 21061267
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (132)1-2 2011
Abstract
We describe 3 non-overlapping, nearly contiguous deletions within chromosome 10q22.1q24.32. Three cases were studied by oligoarray comparative genomic hybridization (CGH), cytogenetics, and/or fluorescence in situ hybridization. The array CGH showed de novo deletions: arr 10q22.1q22.2(74,115,795-77,...
|
PMID: 20714122
PDF is available here.
PDF is available here.
Abstract
We here report on the incorporation of the furan moiety at the 2'-position of a uridine through an amido or ureido linker. The resulting modified ODNs display an unprecedented selectivity for cross-linking toward a cytidine opposite the modified residue, forming one specific cross-linked duplex, whi...
|
PMID: 21162525
PDF is available here.
PDF is available here.
Abstract
Nucleic acids have been demonstrated to be versatile nanoscale engineering materials with the construction of dynamic DNA structures, motors, and circuits. These constructions generally rely on the clever use and integration of relatively few reaction mechanisms and design primitives...
|
PMID: 21166410
PDF is available here.
PDF is available here.
AAPS Journal (12)4 2010
Abstract
We developed and validated an ultrasensitive and selective assay for quantification of synthetic 2'-methoxyphosphorothiolate-miRNA in mouse plasma and cell lysate for the first time. The method is based on a hybridization-ligation fluorescence enzyme-linked immunosorbent assay and has provided a lin...
|
PMID: 20625866
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)11 2010
Abstract
A novel approach for selectively encapsulating Cy5 dye molecules via DNA hybridization in cationic phosphorus dendrimers (G(4)(+))/polystyrenesulfonate (PSS) microcapsules has been developed. The hybridization between Cy5 labeled single strand DNA (ssDNA) targets and complementary ssDNA probes was p...
|
PMID: 20844779
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)11 2010
Abstract
A simple and sensitive approach for the recognition of single base mismatches in duplex DNA was developed. The single base mismatched double-strand (ds) DNA and the completely complementary dsDNA can quench the fluorescence of methylene blue to the different values, so the point mutation dsDNA can b...
|
PMID: 20830326
PDF is available here.
PDF is available here.
Abstract
We report a clinical isolate of Enterococcus faecium in which three distinct and excision-proficient copies of Tn916-like elements are present in the genome. All of the elements contain tet(M) genes, at least one of which confers resistance to tetracycline and minocycline. Two elements (Tn6085a, Tn6...
|
PMID: 20600284
PDF is available here.
PDF is available here.
Abstract
Streptococcus mutans and Streptococcus sobrinus are considered the primary organisms responsible for human dental caries. The ability to generate acids and to adapt to low pH conditions is directly associated with the cariogenic potential of these bacteria. To survive acidic conditions, both species...
|
PMID: 20883222
PDF is available here.
PDF is available here.
Abstract
I (ID03-0561(T)) showed low relatedness to the other strains studied. The three strains in group II (ID03-0784(T), ID03-0808 and ID03-0809) formed a group with high relatedness (98-100 %) and showed low relatedness to the other strains studied. The two strains in group III (ID03-0810(T) and ID03-0...
|
PMID: 19933586
PDF is available here.
PDF is available here.
Abstract
A Gram-negative, motile, non-spore-forming bacterial strain, designated MJ07(T), was isolated from a farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ07(T) belongs to the family Alcali...
|
PMID: 19933592
PDF is available here.
PDF is available here.
Abstract
We propose the names Mucilaginibacter gossypii sp. nov. (type strain Gh-67(T) =NCIMB 14470(T) =KCTC 22380(T)) and Mucilaginibacter gossypiicola sp. nov. (type strain Gh-48(T) =NCIMB 14471(T) =KCTC 22379(T))....
|
PMID: 19946048
PDF is available here.
PDF is available here.
Abstract
Bacterial strains belonging to DNA hybridization groups (HG) II, IV and V, in the Erwinia herbicola-Enterobacter agglomerans complex, of Brenner et al. [Int J Syst Bacteriol 34 (1984), 45-55] were suggested previously to belong to the genus Pantoea, but have never been formally described and classif...
|
PMID: 19946052
PDF is available here.
PDF is available here.
Abstract
A haloalkaliphilic archaeon, strain JX313(T), was isolated from a saline-alkaline soil from Daqing, Heilongjiang Province, China. Its morphological, physiological and biochemical features and 16S rRNA gene sequence were determined. Colonies of the strain were orange-red and cells were non-motile coc...
|
PMID: 19915113
PDF is available here.
PDF is available here.
Abstract
These results and the differentiating biochemical and chemotaxonomic properties show that strain R4-1A(T) represents a novel species, for which the name Chryseobacterium culicis sp. nov. is proposed. The type strain is R4-1A(T) (=LMG 25442(T) =CCM 7716(T))....
|
PMID: 19933582
PDF is available here.
PDF is available here.
Abstract
A polyphasic taxonomic study of a halotolerant bacterium, isolated from sandy rhizospheric soil in Sarbandar, Persian Gulf, Iran, revealed that strain HM6(T) represents a novel species within the genus Nocardiopsis. Results of the 16S rRNA gene sequence comparison revealed that strain HM6(T) cluster...
|
PMID: 19933587
PDF is available here.
PDF is available here.
Abstract
Three strains of Gram-negative, rod-shaped, non-spore-forming bacteria (M 2040(T), M 1973 and M 1878-SK2), isolated from milk of camels at a camel-milk production farm in the United Arab Emirates, were investigated for their taxonomic allocation. On the basis of 16S rRNA gene sequence similarities,...
|
PMID: 19933583
PDF is available here.
PDF is available here.
Abstract
A Gram-positive, endospore-forming, rod-shaped bacterium, strain PD-A10(T), was isolated from salted crab (poo-khem) in Thailand and subjected to a taxonomic study. Phenotypic and chemotaxonomic characteristics, including phylogenetic analyses, showed that the novel strain was a member of the genus...
|
PMID: 19933584
PDF is available here.
PDF is available here.
Abstract
A bacterial strain, SPC26(T), was isolated from a sediment sample of the Southern Ocean off Antarctica. The strain was Gram-staining- and catalase-positive and contained lysine and alanine in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C₁₅:₀ (54.92 %), iso-C₁...
|
PMID: 19783612
PDF is available here.
PDF is available here.
Abstract
A Gram-negative, rod-shaped, non-spore-forming, strictly aerobic strain with gliding motility, designated JAMB N27(T), was isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of 219 m. Strain JAMB N27(T) contained MK-6 as the major isoprenoid quinone and iso-C...
|
PMID: 19915102
PDF is available here.
PDF is available here.
Abstract
A short coccoid- to rod-shaped, motile, mesophilic actinobacterium, strain MSL-22(T), was isolated from soil on Bigeum Island, Korea. A polyphasic study was undertaken to establish the taxonomic position of this strain. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MSL-...
|
PMID: 19915109
PDF is available here.
PDF is available here.
Abstract
We propose the name Vogesella mureinivorans sp. nov. The type strain is 389(T) (=DSM 21247(T) =LMG 25302(T))....
|
PMID: 19946047
PDF is available here.
PDF is available here.
Abstract
Nine Gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria were isolated from pigs affected by different pathological processes. Phenotypic and genotypic methods were adopted to determine the relationships of these new isolates to recognized species of the genus Moraxella. Analysis o...
|
PMID: 19946050
PDF is available here.
PDF is available here.
Genetics in Medicine (12)10 2010
Abstract
The short arm of chromosome 16 is rich in segmental duplications, predisposing this region of the genome to a number of recurrent rearrangements. Genomic imbalances of an approximately 600-kb region in 16p11.2 (29.5-30.1 Mb) have been associated with autism, intellectual disability, congenital anoma...
|
PMID: 20808231
PDF is available here.
PDF is available here.
Abstract
Three strains of fructophilic lactic acid bacteria were isolated from flowers in South Africa. The isolates formed a subcluster in the Lactobacillus buchneri phylogenetic group, closely related to Lactobacillus fructivorans, Lactobacillus homohiochii, Lactobacillus lindneri and Lactobacillus sanfran...
|
PMID: 19965998
PDF is available here.
PDF is available here.
Abstract
Three methods for the detection of HPV DNA were compared in cervical cytologic specimens: the Digene Hybrid Capture II Assay (HC), the Roche Linear Array HPV Genotyping Assay (LA) and the Kurabo GeneSquare Microarray (GS). The main goals of the study were to correlate cytology with HPV detection and...
|
PMID: 20670658
PDF is available here.
PDF is available here.
Abstract
We employ a modified signal probe containing a methylene blue (a redox moiety) label and a "sticky end." When a DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target leading to the creation of a supersandwich structure containing multiple labels. This leads...
|
PMID: 20873767
PDF is available here.
PDF is available here.
Nanotechnology (21)35 2010
Abstract
We demonstrated enhanced localized surface plasmon resonance (SPR) biosensing based on subwavelength gold nanoarrays built on a thin gold film. Arrays of nanogratings (1D) and nanoholes (2D) with a period of 200 nm were fabricated by electron-beam lithography and used for the detection of avian infl...
|
PMID: 20693616
PDF is available here.
PDF is available here.
Abstract
We compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficienc...
|
PMID: 20628872
PDF is available here.
PDF is available here.
Abstract
Excision repair cross-complementing 1-118 single nucleotide polymorphisms (SNPs) have been reported as predictive markers of response to platinum-based chemotherapy. Currently, the most used methods for genotyping of SNPs are costly and time consuming. It is necessary to develop a method that is mor...
|
PMID: 20736746
PDF is available here.
PDF is available here.
Abstract
Fetal disorders (including congenital malformations) are among the most frequent of causes of intrauterine death. Assessment to detect fetal processes is straightforward: history (prenatal, perinatal, and family); external clinical examination; photographs; whole body radiographs; cytogenetic invest...
|
PMID: 20661049
PDF is available here.
PDF is available here.
Abstract
DNA piezoelectric biosensors have become a promising tool in molecular medicine since they do not require any label or staining. Here, a DNA piezoelectric biosensor based on a quartz crystal microbalance (QCM) was created to identify abnormal genes causing α-thalassemia 1 (SEA deletion).
The functi...
|
PMID: 20578968
PDF is available here.
PDF is available here.
Chemical Communications (46)31 2010
Abstract
Using atomic microscopy imaging, probe DNA sequence self-assemblies developed on Si(100) substrates undergo a conformational transition from an extended stem-loop structure to a double helix; such assemblies readily report on DNA molecular recognition events and should be suitable as a label-free, D...
|
PMID: 20596585
PDF is available here.
PDF is available here.
Abstract
We previously showed the feasibility of using locked nucleic acid (LNA) for flow cytometric-fluorescence in situ hybridization (LNA flow-FISH) detection of a target cellular mRNA. Here we demonstrate how the method can be used to monitor viral RNA in infected cells. We compared the results of the LN...
|
PMID: 20584898
PDF is available here.
PDF is available here.
Abstract
We report that this hydrogen bonding pattern can be replaced by metal-ligation between specific metal ions and the natural bases. To demonstrate the feasibility of this principle, two linear oligonucleotides and silver ions were examined as models for DNA hybridization assay and adenosine triphospha...
|
PMID: 20597497
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)8 2010
Abstract
We summarize the landscape of recent efforts in electrochemical DNA hybridization detection. We specifically focus on some main areas from where novel work continues to originate: redox active molecules designed for specific interaction with double stranded DNA, DNA mimics to eliminate background el...
|
PMID: 20502780
PDF is available here.
PDF is available here.
Chemical Communications (46)28 2010
Abstract
Stepwise introduction of multiple streptavidin tetramers into a nanoarray formed in a stick-like punched DNA origami as well as selective removal of predetermined tetramer from the array were successfully achieved by applying a programmed strand displacement technique to a DNA origami scaffold.
|
PMID: 20532403
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
This is the first study dealing with the association between HLA alleles and cystic echinococcosis (CE) in Yemeni patients. The present study aimed to detect the association of HLA-DRB(1) alleles and susceptibility or resistance to CE in Yemeni patients by HLA-DRB(1) typing; first by HLA-DRB(1) ampl...
|
PMID: 20424860
PDF is available here.
PDF is available here.
Abstract
Synthetic nucleic acid mimics provide opportunity for redesigning the specificity and affinity of hybridization with natural DNA or RNA. Such redesign is of great interest for diagnostic applications where it can enhance the desired signal against a background of competing interactions. This report c...
|
PMID: 20572663
PDF is available here.
PDF is available here.
Nanotechnology (21)25 2010
Abstract
A specific colorimetric DNA detection method based on oligonucleotide functionalized gold-silver-alloy nanoparticles (AuAg-alloy-nanoprobes) is presented. The AuAg-alloy-nanoprobes were then used for the specific detection of a DNA sequence from TP53-a gene involved in cancer development. The AuAg-a...
|
PMID: 20508311
PDF is available here.
PDF is available here.
Abstract
We report SNP and CNV haplotype structures determined by analysis of 100 CHMs from Japanese subjects via high-density DNA arrays. The obtained haplotype map should be useful as a reference for the haplotype structure of Asian populations. We resolved common CNV regions (merged CNV segments across th...
|
PMID: 20537301
PDF is available here.
PDF is available here.