Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Isotope Labeling (0):
Recent article
Isotope Labeling
Abstract
| < More recent | Older article > |
Abstract
We propose a new approach of P-selectin molecular imaging based on radiolabeled fucoidan....
|
PMID: 21849401
PDF is available here.
PDF is available here.
Abstract
We report results from a three-year study of the transformations and flows of applied S(0) in soils, vegetation, and hydrologic export pathways of Napa Valley, CA vineyards, documenting that all applied S is lost from the vineyard ecosystem on an annual basis. We found that S(0) oxidizes rapidly to...
|
PMID: 21825150
PDF is available here.
PDF is available here.
Abstract
The Quantitative Assay Database (QuAD), http://proteome.moffitt.org/QUAD/, facilitates widespread implementation of quantitative mass spectrometry in cancer biology and clinical research through sharing of methods and reagents for monitoring protein expression and modification....
|
PMID: 21656910
PDF is available here.
PDF is available here.
Abstract
We characterize labeling efficiency through assessment of FDG uptake and retention by the ASCs and the effect of FDG on cell viability, proliferation, transdifferentiation, and cell function in vitro using rat ASCs.
Samples of 10(5) ASCs (from visceral fat tissue) were labeled with c...
|
PMID: 21380591
PDF is available here.
PDF is available here.
Abstract
We discuss, production, characterization and in-vivo evaluation of nevirapine nanosuspensions. Laser diffraction showed that the average particles size was 457 nm. Following single-dose administration, the plasma gamma concentration profiles showed fast release. Macrophage uptake studies confirmed e...
|
PMID: 21699078
PDF is available here.
PDF is available here.
Abstract
We previously prepared a SREB2-overexpressing transgenic mouse for functional analysis but were unable to confirm SREB2 protein expression level by immunochemical or biochemical methods. In this article, we report mass spectrometric identification and relative quantitative analysis of SREB2 in the f...
|
PMID: 21395347
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We have addressed this limitation by adopting quantitative mass spectrometric approaches to identify proteins that enrich in a purified fraction of organelles relative to a crude or contaminating fraction. The methods for the analyses of the yeast peroxisome are described in detail; however, these c...
|
PMID: 18370030
PDF is available here.
PDF is available here.
Abstract
A stable isotope dilution assay (SIDA) for the Alternaria mycotoxin tenuazonic acid was developed. Therefore, [(13)C(6),(15)N]-tenuazonic acid was synthesized from [(13)C(6),(15)N]-isoleucine by Dieckmann intramolecular cyclization after acetoacetylation with diketene. The synthesized [(13)C(6),(15)N...
|
PMID: 21370870
PDF is available here.
PDF is available here.
Abstract
We performed a clinical experiment in intubated healthy subjects to study the time course of changes in the distribution of all lipid moieties present in duodenal phases during 4 h of digestion of meals with 3.2 g PE (PE meal) or without (control meal) PE. In vitro experiments under simulated gastro...
|
PMID: 21482714
PDF is available here.
PDF is available here.
Abstract
We recently characterized binding of C1q and factor H to immobilized and liposomal anionic phospholipids. Factor H inhibited C1q binding to anionic phospholipids, suggesting a role for factor H in regulating activation of the complement classical pathway by anionic phospholipids. To extend this find...
|
PMID: 21574022
PDF is available here.
PDF is available here.
Abstract
We used small interfering RNA (siRNA) to reduce gp78 expression in HepG2 cells. Reduction of gp78 decreased apoB-100 ubiquitination and cytosolic apoB-ubiquitin conjugates. Radiolabeling studies revealed that gp78 knockdown increased secretion of newly synthesized apoB-100 and, unexpectedly, enhance...
|
PMID: 21421992
PDF is available here.
PDF is available here.
Abstract
We report here the identification and complete characterization of a novel oxysterol, 3β,5α-dihydroxycholest-7-en-6-one (DHCEO), as a biomarker for 7-DHC oxidation in fibroblasts from SLOS patients and brain tissue from a SLOS mouse model. Deuterated (d(7))-standards of 7-DHC and DHCEO were synthe...
|
PMID: 21402677
PDF is available here.
PDF is available here.
Abstract
We studied adipose metabolism in 10 baboons (13.0 ± 4.2 years old, 29.5 ± 5.5 kg). Part 1 evaluated the effect of different sedatives on the rate of appearance of plasma free fatty acids (RaFFA), assessed using (13)C(4)-labeled palmitate infusion (7 µmol/kg/min). Animals, were studied with no sed...
|
PMID: 21415122
PDF is available here.
PDF is available here.
Abstract
Louis-Philippe Morin is a senior instrument application specialist at Algorithme Pharma, a CRO located in Laval, Canada. He has been working in the bioanalysis industry for the past 10 years where he became a subject matter expert in analytical instrumentation, especially in the MS f...
|
PMID: 21320048
PDF is available here.
PDF is available here.
Abstract
We investigate ecological resilience in a coastal mangrove community from the Galápagos Islands over the last 2700 years using three research questions: What are the 'fast and slow' processes operating in the coastal zone? Is there evidence for a threshold response? How can the past inform us about...
|
PMID: 21811594
PDF is available here.
PDF is available here.
Abstract
We describe a workflow for proteomic semi-quantitative expression profiling of liver from rats treated with a known hepatotoxicant using a multiplexed isobaric labeling strategy and multi-dimensional liquid chromatography....
|
PMID: 20972766
PDF is available here.
PDF is available here.
Arzneimittel-Forschung (61)2 2011
Abstract
Dermal absorption of mucopolysaccharide polysulfate (MPS, the active ingredient of Hirudoid") in human and minipig was investigated by using 14C-labeled MPS. Three types of human and minipig skin samples were used: intact, dried and tape-stripped. At 24 h after application of 14C-MPS...
|
PMID: 21428242
PDF is available here.
PDF is available here.
Abstract
A versatile synthetic route to prepare all four stereoisomeric 4-fluoro-glutamines was developed by exploiting a Passerini three-component reaction. The skeleton of 4-substituted glutamine derivatives was efficiently constructed. Subsequent four-step reactions, highlighted by a "neut...
|
PMID: 21190335
PDF is available here.
PDF is available here.
Abstract
SH3 is a ubiquitous domain mediating protein-protein interactions. Recent solution NMR structural studies have shown that a proline-rich peptide is capable of binding to the human vinexin SH3 domain. Here, an orthogonal amber tRNA/tRNA synthetase pair for (15)N/(19)F-trifluoromethyl-phenylalanine ((...
|
PMID: 20946873
PDF is available here.
PDF is available here.
Abstract
We report the synthesis and in vitro receptor binding assay of three benzopyrano[3,4-c]pyridin-5-ones as potential dopamine D4 receptor ligands. These new compounds have higher affinity and selectivity toward dopamine D4 receptor and their K(i) values for D4 receptor are in the nanomolar (nM) range....
|
PMID: 21155383
PDF is available here.
PDF is available here.
Nature Methods (7)11 2010
Abstract
We introduce a quantitative proteomics approach to sort out non-cell-autonomous proteins synthesized by other cells or intracellular pathogens. Our approach combines stable-isotope labeling of amino acids in cell culture (SILAC), high-purity cell sorting and bioinformatics analysis to identify the r...
|
PMID: 20935649
PDF is available here.
PDF is available here.
ISME Journal (4)11 2010
Abstract
Lonar Lake is a unique saline and alkaline ecosystem formed by meteor impact in the Deccan basalts in India around 52,000 years ago. To investigate the role of methylotrophy in the cycling of carbon in this unusual environment, stable-isotope probing (SIP) was carried out using the o...
|
PMID: 20555363
PDF is available here.
PDF is available here.
Current protocols in protein science / editorial board, John E. Coligan ... [et al.] (Chapter 14) 2010
Abstract
I (GGTase I), and Rab geranylgeranyl transferase (also termed geranylgeranyltransferase type II - GGTase II). In this unit, synthetic isoprenoids conjugated to either a fluorophore or biotin group are used to assay the activity of protein prenyltransferases in vitro or to affinity tag prenylatable p...
|
PMID: 21104984
PDF is available here.
PDF is available here.
Abstract
A novel synthesis of 4-[(18)F]fluorobenzylamine ([(18)F]FBA) by means of transition metal-assisted sodium borohydride reduction of 4-[(18)F]fluorobenzonitrile ([(18)F]FBN) is described. This approach could successfully be extended to borohydride exchange resin (BER) enabling a viable option for use...
|
PMID: 20740244
PDF is available here.
PDF is available here.
Journal of Lipid Research (51)11 2010
Abstract
These results clearly indicate that ingested dihydroceramide can be incorporated into various tissues, including brain, and metabolized to other sphingolipids....
|
PMID: 20656918
PDF is available here.
PDF is available here.
Abstract
An important challenge for proteomics is the ability to compare protein levels across biological samples. Since their introduction, isotopic and isobaric peptide labeling have played an important role in relative quantitative comparisons of proteomes. One important drawback of most of the isotopic-l...
|
PMID: 20973638
PDF is available here.
PDF is available here.
Joint Bone Spine (77)5 2010
Abstract
In this paper past research on the natural history of Mseleni joint disease, a crippling endemic osteoarthritis, its socio-economic impacts, the demographics, diet, geology and the genetic background of affected people are reviewed. In addition, some new research ideas are suggested to continue the...
|
PMID: 20471895
PDF is available here.
PDF is available here.
Anticancer Research (30)10 2010
Abstract
These results indicated that BLI could be used to monitor the growth of tumour. High uptake of (177)Lu-AMBA in PC-3M-luc-C6 tumour-bearing mice by microSPECT/CT imaging can further evaluate the potential of (177)Lu-AMBA therapy for PC-3M-luc-C6 tumours....
|
PMID: 21036718
PDF is available here.
PDF is available here.
Abstract
We took an unbiased approach to studying IAV-nucleolar interactions by using stable isotope labeling with amino acids in cell culture (SILAC) in conjunction with LC-MS/MS to quantify changes in the nucleolar proteome following infection with A/PR/8/34 (H1N1) and A/Udorn/72 (H3N2) strains of the viru...
|
PMID: 20701360
PDF is available here.
PDF is available here.
Journal of Proteomics (73)10 2010
Abstract
In the field of proteomics there is an apparent lack of reliable methodology for quantification of posttranslational modifications. Present study offers a novel post-digest ICPL quantification strategy directed towards characterization of phosphorylated and glycosylated proteins. The...
|
PMID: 20601274
PDF is available here.
PDF is available here.
Journal of Proteomics (73)10 2010
Abstract
T cell epitopes are peptides, for instance derived from foreign, mutated or overexpressed proteins, that are displayed by MHC molecules on the cell surface and that are recognized by T lymphocytes. Knowledge of the identity of epitopes displayed by MHC molecules is of high value for...
|
PMID: 20546957
PDF is available here.
PDF is available here.
Journal of Proteomics (73)10 2010
Abstract
We describe a number of established technologies for the enrichment of accessible vascular proteins based on the isolation of glycoproteins, the in vivo coating of accessible cell surfaces with colloidal silica and the in vivo perfusion with reactive ester derivatives of biotin. Label-free as well a...
|
PMID: 20538087
PDF is available here.
PDF is available here.
Abstract
Biomarkers for colorectal cancer (CRC) early diagnosis are currently lacking. The purpose of this study was to interpret molecular events in the early stage of CRC that may bring about new biomarkers for early diagnosis. Methylation isotope labeling assistant gel-enhanced liquid chromatography-mass...
|
PMID: 20666480
PDF is available here.
PDF is available here.
Abstract
Cancer progression is governed by multifaceted interactions of cancer cells with their microenvironment and one of these ways is through secreted compounds. Substances released by gastric cancer cells have not being profiled in a proteome-wide manner. ITRAQ-based tandem mass spectrom...
|
PMID: 20812763
PDF is available here.
PDF is available here.
Abstract
Positron emission tomography (PET) is a powerful and rapidly developing area of molecular imaging that is used to study and visualize human physiology by the detection of positron-emitting radiopharmaceuticals. Information about metabolism, receptor/enzyme function, and biochemical mechanisms in liv...
|
PMID: 20497120
PDF is available here.
PDF is available here.
Abstract
The current way to increase efficacy of cancer therapy is the use of molecular recognition of aberrantly expressed gene products for selective treatment. However, only a fraction of the patients have tumors with a particular molecular target. Radionuclide imaging of molecular targets might help to s...
|
PMID: 20497119
PDF is available here.
PDF is available here.
Abstract
We present a theoretical study of the possibility to use isotope label two-dimensional infrared (2DIR) spectroscopy to obtain site specific structural information in trpzip2. This small beta-hairpin peptide was designed as a model system for studying protein folding in beta-sheet structures. In orde...
|
PMID: 20596553
PDF is available here.
PDF is available here.
Abstract
We provide analytical solutions for the S-REDOR and S-RESPDOR dephasing curves. These solutions facilitate the measurement of hetero-nuclear distances from experimental data....
|
PMID: 20577687
PDF is available here.
PDF is available here.
Abstract
The stable isotope dilution measurement of bile acid pool sizes and turnover rates in humans has involved the collection of nine blood samples over four days. This precludes widespread application to larger population studies. This study describes a two time-point approach for blood sampling without...
|
PMID: 20645205
PDF is available here.
PDF is available here.
Abstract
We compared these IS surrogates to the gold standard (euglycemic-hyperinsulinemic clamp). Thirty (15 male/15 female) sedentary, nondiabetic participants (27.2 +/- 4.0 (s.d.) kg/m(2), 35 +/- 12 years) were admitted for 3 days to our in-patient unit. After a 10-h fast, they received 60 g of glucose an...
|
PMID: 20360760
PDF is available here.
PDF is available here.
Bioanalysis (2)9 2010
Abstract
The ultimate objective of clinical proteomics is the successful discovery, validation and translation of biomarkers, together with new therapeutic targets into medical practices. New highly developed technologies in proteomics and their use in understanding tumor biology have signifi...
|
PMID: 21083289
PDF is available here.
PDF is available here.
Proteomics (10)17 2010
Abstract
We have used iTRAQ proteomics to identify and quantify alterations in global protein expression of T. forsythia during growth in a biofilm. This is the first proteomic study concentrating on biofilm growth in this key periodontal pathogen, and this study has identified several changes in protein exp...
|
PMID: 20806225
PDF is available here.
PDF is available here.
Abstract
We describe the in vitro and in vivo evaluation of DTPA-derivatized lipid-based NP (DTPA-NP) radiolabeled with different radiometals, including (111)In and (99m)Tc, for single-photon emission computed tomography (SPECT), (68)Ga for positron emission tomography (PET), and (177)Lu for therapeutic appl...
|
PMID: 19863193
PDF is available here.
PDF is available here.
Abstract
We evaluated several open-source analysis tools in terms of performance on two reference data sets, explicitly generated for this purpose. In this paper we present an implementation, T3PQ (Top 3 Protein Quantification), of the method suggested by Silva and colleagues for LC-MS(E) applications and we...
|
PMID: 20576481
PDF is available here.
PDF is available here.
Abstract
I band (approx. 45 cm(-1) towards lower frequency) upon (13)C labelling of one of the proteins reveals the amide I band of the unlabelled protein, enabling it to be used as a probe for monitoring conformational changes. With site-specific isotopic labelling, structural resolution at the level of ind...
|
PMID: 20658981
PDF is available here.
PDF is available here.
Abstract
We quantified and identified 31 different proteins which include AGP, complement C4, haptoglobin, alpha-1-antitrypsin precursor, alpha-2-macroglobulin precursor, prothrombin precursor and ubiquitin carboxyl-terminal hydrolase 44 (USP44), etc. The serum concentration of AGP in HCC patients (n = 52) w...
|
PMID: 20130913
PDF is available here.
PDF is available here.
Abstract
We present QTIPS, Quantification by Total Identified Peptides for SILAC, a straightforward, accurate method to determine the level of heavy amino acid incorporation throughout a population of peptides detected by mass spectrometry. Using QTIPS, we show that the incorporation of heavy amino acids in...
|
PMID: 20451407
PDF is available here.
PDF is available here.
Proteomics (10)16 2010
Abstract
We demonstrate MM (RP-AX) HPLC as an alternative approach for shotgun proteomic studies that offers significant advantages over traditional SCX peptide separations....
|
PMID: 20662100
PDF is available here.
PDF is available here.
Journal of Nutrition (140)8 2010
Abstract
Our primary aim in this study was to validate cross-sectional time series (CSTS) and multivariate adaptive regression splines (MARS) models based on observable participant characteristics, heart rate (HR), and accelerometer counts (AC) for prediction of minute-by-minute EE, and hence 24-h total EE (...
|
PMID: 20573939
PDF is available here.
PDF is available here.
Abstract
Aminolaevulinic acid (ALA) is known to poorly penetrate into thick lesions, such as nodular basal cell carcinomas. Short chain ALA esters, possessing increased lipophilicity relative to their hydrophilic parent, have previously been shown to be highly efficient at inducing protoporph...
|
PMID: 20222026
PDF is available here.
PDF is available here.
Analytical Biochemistry (404)2 2010
Abstract
We have been using the method which relies on the base-catalyzed exchange of hydrogen (deuterium) between water and acetone. 2H-labeling of acetone is then determined using GCMS. Although not noted in the original paper, when chloroform is used to extract the acetone there is slow but substantial ba...
|
PMID: 20488158
PDF is available here.
PDF is available here.
Analytical Biochemistry (404)1 2010
Abstract
We developed a method to distinguish and quantify C1 and C2 (13)C-labeled glucose by derivatization with methylamine followed by multiple reaction monitoring scans in a Q-trap mass spectrometer. Fragment ion ratios in the tandem mass spectra showed an isotope effect with (13)C or deuterium labeling,...
|
PMID: 20447372
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We demonstrate that (1-->3),(1-->4)-beta-D-glucans are detected immunocytochemically at the Golgi of the developing maize coleoptiles. Further, when maize seedlings at the third-leaf stage were pulse labeled with [(14)C]O(2) and Golgi membranes were isolated from elongating cells at the base of the...
|
PMID: 20488897
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We have established and validated a liquid chromatography tandem mass spectrometry method using multiple reaction monitoring mode to separate and quantify the levels of plant cell wall precursors. Target analytes were identified by their parent/daughter ions and retention times. The method allows th...
|
PMID: 20442274
PDF is available here.
PDF is available here.
Abstract
Overexpression of HER2 receptors is a prognostic and predictive biomarker in breast cancer and a number of other malignancies. Radionuclide molecular imaging of HER2 overexpression may influence patient management making treatment more personalized. Earlier, (111)In-DOTA-Z(HER2:342-p...
|
PMID: 20130858
PDF is available here.
PDF is available here.
Abstract
We use this feature to develop a simple protocol for FFPE analysis. Combination with simple pipet-tip based peptide fractionation identified about 5000 mouse liver proteins in 24 h measurement time-the same as in fresh tissue. Results from the FFPE-FASP procedure do not indicate any discernible chan...
|
PMID: 20469934
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1801)7 2010
Abstract
We carried out a set of labelling experiments with C6 rat glioma cells in culture. TAG and phospholipid metabolism was monitored by incubating C6 cells with [1-(13)C]-glucose at two time points during cell growth curve -24 h incubation starting at log-phase; 48 h incubation starting at saturation de...
|
PMID: 20380892
PDF is available here.
PDF is available here.
Veterinary Microbiology (143)1 2010
Abstract
Infection of target cells by alphaherpesviruses leads to extensive modulation of host cell gene expression. To gain detailed information on the molecular pathways affected by infection of Madin-Darby bovine kidney (MDBK) cells with PrV, transcript analysis was combined with a stable isotope-based qu...
|
PMID: 20233642
PDF is available here.
PDF is available here.
Abstract
We demonstrate that treating these quantities as unknown parameters can reduce bias in the estimated source contributions, although model complexity is increased (thereby increasing the variance of estimates). The advantages of this fully Bayesian approach are particularly apparent when the source g...
|
PMID: 20496928
PDF is available here.
PDF is available here.