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Preservation, Biological (3):
Recent article
Preservation, Biological
Abstract
We characterized plants and soils from an abandoned Pb-Zn mine in the Basque Country (northern Spain). Soil in this area proved to be deficient in major macronutrients and to contain toxic levels of Cd, Pb, and Zn. Spontaneously growing native plants (belonging to 31 species, 28 genera, and 15 famil...
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PMID: 21598791
PDF is available here.
PDF is available here.
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Abstract
The storage of the different microorganisms over long periods is necessary to ensure reproducible results and continuity in research and in biomedical processes and also for commercial purposes. Effective storage means that a microorganism is maintained in a viable state free of contamination or gen...
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PMID: 20949399
PDF is available here.
PDF is available here.
Journal of Trauma (69)5 2010
Abstract
Splenic artery angioembolization (SAE) is increasingly being used as an adjunct to nonoperative management for stable patients with blunt splenic injury (BSI). However, little is known about splenic immunocompetence after SAE. This study aims at assessing splenic immunocompetence after SAE for BSI....
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PMID: 21068617
PDF is available here.
PDF is available here.
Abstract
A wide range of methods are commonly used for preserving environmental samples prior to molecular analyses. However, the effect of these preservation methods on fungal DNA is not understood. The objective of this study was to test the effect of eight different preservation methods on the quality and...
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PMID: 20470836
PDF is available here.
PDF is available here.
Abstract
Abstract
We investigated the survival mechanisms of freeze-dried or liquid-dried (L-dried) yeast cells in ampoules. Type strains of various yeasts were freeze-dried or L-dried and sealed in ampoules under high vacuum (< 1 Pa) or low vacuum (4.8 x 10(4) Pa), then stored at 37 degrees C (accelerated storage te...
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PMID: 20513958
PDF is available here.
PDF is available here.
Abstract
Fourteen isolates of basidiomycete decay fungi (12 species) were maintained for 18 years on agar slants transferred annually and also stored as mycelium-agar cores under cold sterile water without subculture. Isolates stored by each method were evaluated for decay effectiveness using a standard labo...
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PMID: 20453914
PDF is available here.
PDF is available here.
Abstract
We have obtained dramatically improved results with a simple modification of a standard protocol. Cells growing on a coverslip are rapidly dehydrated in a cold organic solvent and then are rehydrated in a solution containing a homobifunctional crosslinker. The crosslinking reaction stabilizes cellul...
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PMID: 20419424
PDF is available here.
PDF is available here.
Abstract
The levels of CD62P and sHLA-1 were significantly lower in leucoreduced PC than in non-leucoreduced ones. However, the overall rate of increase of sHLA-1 during storage was faster in the leucoreduced group of PC. No significant differences were detected regarding other assays of quality. CONCLUSION:...
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PMID: 20104276
PDF is available here.
PDF is available here.
Abstract
We evaluated two novel products for room temperature DNA storage: Biomatrica's DNA SampleMatrix technology and GenVault's GenTegra DNA technology. We compared the integrity and quality of DNA stored using these products against DNA stored in a -20 C freezer by performing downstream testing with shor...
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PMID: 19801719
PDF is available here.
PDF is available here.
Abstract
Modern dental practice offers a wide variety of treatment alternatives and modalities. With the wider scale of treatment options, new dilemmas emerged. A common dilemma is the decision whether to preserve the natural tooth by endodontic treatment, or to extract the tooth and replace it with a single...
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PMID: 20597259
PDF is available here.
PDF is available here.
New Yorker () 2010
Abstract
Abstract
To improve the diagnosis efficiency of human strongyloidiasis by using formalin-ether concentration technique (FECT), the effects of various factors on the recovery rates of Strongyloides stercoralis larvae were comparatively evaluated. Fresh stool and a short time exposure of larvae to formalin yie...
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PMID: 19923489
PDF is available here.
PDF is available here.
Abstract
Abstract
Cell culture is the most popular method of virus propagation because of its high sensitivity. However, the need of high cost liquid nitrogen for storage of cell lines is one of the main limiting factor for its widespread use in developing countries.
The present study was therefore carried out to sta...
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PMID: 21045405
PDF is available here.
PDF is available here.
Journal of Dairy Science (93)1 2010
Abstract
Six Holstein heifers (body weight=535-625 kg) fed a total mixed ration containing either high protein (13.4%) or low protein (9.0%) were used to evaluate the effect of 3 urine collection methods (chilled, acidified before collection, or acidified after 6h of collection) on urinary N preservation. In...
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PMID: 20059930
PDF is available here.
PDF is available here.
Lakartidningen (107)21 2010
Abstract
Abstract
The cost-effectiveness of prescreening for alanine aminotransferase (ALT) activity was evaluated in blood donors before donation. Since September 15, 2006, the Krasnodar Territory blood transfusion station has examined more than 32 thousand donors in this fashion. A Reflotron Plus biochemical analyz...
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PMID: 20030265
PDF is available here.
PDF is available here.
Proteomics (9)20 2009
Abstract
We have evaluated the effects of trichloroacetic acid (TCA) and thermal treatments prior to protein extraction as a means to minimise proteolysis. TCA precipitation is commonly used to inactivate proteases; thermal stabilisation is used to heat samples to approximately 95 degrees C to inactivate enz...
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PMID: 19824011
PDF is available here.
PDF is available here.
Abstract
I was the control. The bones in Group II underwent the state of being deeply frozen mean while having the rapid temperature change during the preparation. In Group III, the bones underwent the state of being deeply frozen and having the slow temperature change during the preparation. Group IV was th...
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PMID: 19894334
PDF is available here.
PDF is available here.
Abstract
(31)P NMR spectroscopy has been used to elucidate the role of glass-forming sugars in the preservation of dipalmitoylphosphatidylcholine (DPPC) lipid bilayers. (31)P wideline NMR spectra of freeze-dried pure DPPC, DPPC/trehalose, DPPC/glucose, and DPPC/hydroxyethyl starch (HES) mixtures collected in...
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PMID: 19604010
PDF is available here.
PDF is available here.
Parasitology (90 ( Pt 3))03 2009
Abstract
Parasitology (90 ( Pt 3))03 2009
Abstract
The modified Kato technique has the advantages of reproducibility, simplicity and economy: the disadvantage is that it cannot be used in conjunction with traditional faecal preservatives. Sodium azide has been evaluated as a preservative for human faeces for subsequent Kato analysis. More than 400 f...
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PMID: 4011316
PDF is available here.
PDF is available here.
British Journal for the History of Science (42)152 Pt 2 2009
Abstract
This paper sketches how late seventeenth-century Dutch anatomists used printed publications to advertise their anatomical preparations, inventions and instructional technologies to an international clientele. It focuses on anatomists Frederik Ruysch (1638-1732) and Lodewijk de Bils (1624-69), invent...
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PMID: 19852263
PDF is available here.
PDF is available here.
Journal of Virological Methods (156)1-2 2009
Abstract
We demonstrated that RNA can be extracted from cervical cell lines and cytology specimens stored in BD SurePath preservative fluid with three different procedures that all contain proteinases. This RNA is suitable for real-time RT-PCR applications....
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PMID: 19095009
PDF is available here.
PDF is available here.
Abstract
We varied the culture conditions (i.e., the NaCl content of the medium), storage protection media, and preservation methods (vacuum drying vs. encapsulation gels remaining hydrated) in order to achieve optimal preservation of the activity of As (III) bioreporter bacteria during up to 12 weeks of sto...
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PMID: 19198829
PDF is available here.
PDF is available here.
Journal of Parasitology (95)1 2009
Abstract
We have previously shown that parasite eggs have been identified in the coprolites of Korean mummies. These eggs have shed light on parasitic infection patterns in Korean populations living several hundred years ago. We conducted a scanning electron microscopy (SEM) study on ancient Trichuris trichi...
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PMID: 18601576
PDF is available here.
PDF is available here.
Molecular Vision (15) 2009
Abstract
The current study demonstrated the importance of proper isolation techniques to obtain the maximum amount of intact photoreceptor cells. The data suggested that a gentle dissociation technique, consisting of enzymatic treatment followed by moderate pipeting of the retinal tissue, may be the key to o...
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PMID: 19137071
PDF is available here.
PDF is available here.
Abstract
Abstract
We include data which suggest that the common marmoset (Callithrix jacchus) monkey, a New World primate, exhibits important parallels with human testicular development and may help us to understand why the pre-pubertal testis is vulnerable to effects of cytotoxic therapy on future fertility.
Copyrig...
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PMID: 19293606
PDF is available here.
PDF is available here.
Abstract
We will first address normal female fertility and methods to detect decreased fertility. Hence we will focus on direct effects as well as late fertility-related adverse effects caused by chemotherapy and radiotherapy, and we will conclude with a summary of current options for fertility preservation...
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PMID: 19293607
PDF is available here.
PDF is available here.
Abstract
It is now known that the electric charge of a surface cellular membrane is a rather characteristic value for each type of a cell. Cellular electrophoresis may be used to study the electrokinetic properties of red blood cells in each specific case, thereby obtaining indirect information on the state...
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PMID: 19253696
PDF is available here.
PDF is available here.
Abstract
BCC and S. maltophilia can be identified with relative ease using a small battery of biochemical reactions. Use of simplified methods will allow greater recognition of their pathogenic potential and correct antimicrobials should be advised in other clinical laboratories and hospitals....
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PMID: 19384035
PDF is available here.
PDF is available here.
Abstract
Vaccination is a highly effective method and a cheap tool for preventing certain infectious diseases. Routine immunization programs protect most of the world's children from diseases that claim millions of lives each year. There are many practical problems impeding vaccine delivery, especially to ma...
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PMID: 19340694
PDF is available here.
PDF is available here.
Abstract
Hydrolytic enzymes secreted by fungi play an important role in the pathogenesis of infection. With the aim of evaluating the enzymatic activity, 31 isolates of Acremonium stored in the University of Recife Mycology (URM) Culture Collection were tested. Culture fragments were transferred to glycoside...
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PMID: 19287938
PDF is available here.
PDF is available here.
Abstract
Our experience indicates that storage of M. tuberculosis on glass beads at -70 degrees C is a suitable procedure for an active culture collection in a public health laboratory like ours, where maintenance of M. tuberculosis cultures is a complementary activity and must be quick, practical, effective...
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PMID: 19201741
PDF is available here.
PDF is available here.
Transplantation (86)11 2008
Abstract
Accounting for the variations in vascular and ductal anatomy, as detailed in this study, will facilitate development of protocols for preservation, optimal enzyme administration, and pancreas distention and digestion, and will ultimately lead to substantial improvements in isolation outcomes....
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PMID: 19077881
PDF is available here.
PDF is available here.
Abstract
Dermal substitutes can be used to improve the wound healing of deep burns when placed underneath expanded, thin autologous skin grafts. Such dermal matrix material can be derived from xenogeneic or human tissue. Antigenic structures, such as cells and hairs must be removed to avoid adverse inflammat...
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PMID: 18491218
PDF is available here.
PDF is available here.
Cryobiology (57)3 2008
Abstract
Conventional cold storage and cryopreservation methods for hops (Humulus lupulus L.) are available but, to our knowledge, the genetic and epigenetic stability of the recovered plants have not been tested. This study analyzed 51 accessions of hop using the molecular techniques, Random Amplified DNA P...
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PMID: 18822279
PDF is available here.
PDF is available here.
Current Biology (18)21 2008
Abstract
We have retrieved the first complete mitochondrial-genome sequence of a prehistoric European. We have then compared it with 115 related extant lineages from mitochondrial haplogroup K. We found that the Iceman belonged to a branch of mitochondrial haplogroup K1 that has not yet been identified in mo...
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PMID: 18976917
PDF is available here.
PDF is available here.
Abstract
Gilbert et al. (Reports, 28 September 2007, p. 1927) reported that "hair shafts surpass comparably stored bone as an aDNA source [...] in regard to preservation and concentration of mtDNA." When experimental parameters are carefully controlled for, including adequate sampling, quantitative polymeras...
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PMID: 18988826
PDF is available here.
PDF is available here.
Abstract
Both approaches resulted in the rapid procurement and retrieval of well-preserved cells and nucleic acids. The presence of "contaminating" normal cells in the more traditional approach masked the significance of genetic gains and losses, findings that were more readily apparent from the material der...
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PMID: 18629512
PDF is available here.
PDF is available here.
Water Research (42)17 2008
Abstract
We observed neither index functions nor a predictive relationship between any of microbial indicators and viable Cryptosporidium oocysts. In contrast, several regression models (r>0.6) and discriminant functions (67-88% well classified samples) based mostly on numbers of bacteriophages were able to...
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PMID: 18762313
PDF is available here.
PDF is available here.
Abstract
These results demonstrated the different responses of anti-oxidative mechanism between normoxia and hypoxia. Additionally, the present study suggested that the GSH-GPX antioxidant system played an important role in HSPCs preservation by reducing peroxidation....
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PMID: 18625331
PDF is available here.
PDF is available here.
Abstract
Parasitology Research (103)5 2008
Abstract
A new method of storage of the free-living amoeba, Acanthamoeba, is described. For this purpose, strips of filter paper were impregnated with cysts of amoebas cultivated axenically and transferred into a cryotube. The strips were dehydrated at 30 degrees C for 24 h and after storage at room temperat...
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PMID: 18618145
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (331)10 2008
Abstract
We also propose that changes in ROS homeostasis would play a role in perception of environmental factors by seeds during their germination, and thus act as a signal controlling the completion of germination. However, uncontrolled accumulation of ROS is likely to occur during seed aging or seed desic...
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PMID: 18926495
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (331)10 2008
Abstract
The seed constitutes the main vector of plant propagation and it is a critical development stage with many specificities. Seed longevity is a major challenge for the conservation of plant biodiversity and for crop success. Seeds possess a wide range of systems (protection, detoxification, repair) al...
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PMID: 18926494
PDF is available here.
PDF is available here.
Abstract
We examined potential biases introduced by DNA preservation and extraction methods by comparing the quality, quantity, and diversity of environmental DNA samples preserved and extracted by commonly used methods. We included samples from sites representing the spectrum of environmental conditions tha...
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PMID: 18633656
PDF is available here.
PDF is available here.
Clinica Chimica Acta (396)1-2 2008
Abstract
The results of this study suggest that blood samples for Bf measurement should be analyzed within 2 weeks after collection and storage at -20 degrees C....
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PMID: 18644357
PDF is available here.
PDF is available here.
Abstract
Brain banks are facilities providing an interface between generous donation of nervous tissues and research laboratories devoted to increase our understanding of the diseases of the nervous system, discover new diagnostic targets, and develop new strategies. Considering this crucial role, it is impo...
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PMID: 18543077
PDF is available here.
PDF is available here.
Abstract
Hyperbaric oxygen therapy is a technology that involves oxygen treatment at supra-atmospheric pressures in high concentrations, generating increased levels of physically dissolved oxygen in blood plasma. This form of transported oxygen, compared with oxygen chemically bound to hemoglobin, is able to...
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PMID: 18672481
PDF is available here.
PDF is available here.
Abstract
Vegetatively colonized agar cores of 69 basidiomycete fungus isolates (48 species in 30 genera and 17 families) were stored at 5 degrees C in tubes of sterile distilled water without manipulation for 20 years. These were represented by 34 isolates of saprotrophic fungi (29 species in 19 genera) and...
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PMID: 18772921
PDF is available here.
PDF is available here.
Abstract
Dry preservation involves removing water from samples so that degradative biochemical processes are slowed and extended storage is possible. Recently this approach has been explored as a method for preserving living mammalian cells. The current work explores the use of microwave processing to enhanc...
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PMID: 18318445
PDF is available here.
PDF is available here.
Journal of Virological Methods (150)1-2 2008
Abstract
In the collection of field materials to test for the presence of arboviruses, samples must be appropriately maintained to detect arboviral nucleic acids. In austere field conditions this is often difficult to achieve because, during routine specimen processing, storage, and shipping viral RNA degrad...
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PMID: 18387678
PDF is available here.
PDF is available here.
Journal of Parasitology (94)3 2008
Abstract
Although cryopreservation protocols for storage of hookworm larvae have been described, the circumstances under which the technique is necessary to ensure larval survival are not well defined. The motility of infective-stage larvae (as judged by observation) and their ability to migrate through cani...
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PMID: 18605779
PDF is available here.
PDF is available here.
Abstract
Bacillus anthracis spore suspensions can be stored for long periods of time without significant loss of viability or clumping. The content of ES DNA was variable and changed with time. SIGNIFICANT AND IMPACT OF THE STUDY: The study shows that BA spore suspensions can be developed for reference mater...
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PMID: 18194254
PDF is available here.
PDF is available here.
Annals of Clinical Biochemistry (45)Pt 3 2008
Abstract
These data show that NT-proBNP can be stored for at least two years at -20 degrees C before measurement without a substantial loss of immunoreactivity....
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PMID: 18482923
PDF is available here.
PDF is available here.
Journal of Medical Microbiology (57)Pt 5 2008
Abstract
Abstract
OBJECTIVE: To investigate effects of different drying methods on the content of anthraquinones and tannins in water extract from Radix et Rhiroma Rhei (DHY). METHOD: DHY was dried by freeze drying, vacuum drying, drying under normal press and spray drying respectively until its moisture has been 5%....
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PMID: 18619345
PDF is available here.
PDF is available here.