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Enzyme Stability (0):
Recent article
Enzyme Stability
Abstract
Our purification scheme led to a 30-fold purification of this activity, with the removal of more than 99.9% of total protein. While Ado was the preferred substrate, inosine and guanosine were also cleaved, with 43% and 32% of the Ado activity, respectively. Our data suggest that M. smegmatis express...
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PMID: 21821769
PDF is available here.
PDF is available here.
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Abstract
We report that MT4-MMP is displayed on the cell surface as a mixed population of monomeric, dimeric, and oligomeric forms. Sucrose gradient fractionation demonstrated that these forms of MT4-MMP are all present in lipid rafts. Mutational and computational analyses revealed that Cys(564), which is pr...
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PMID: 21828052
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1810)7 2011
Abstract
Multifunctional l-amino acid oxidases (LAAOs) occur widely in snake venoms.
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PMID: 21539897
PDF is available here.
PDF is available here.
Abstract
We also predict that Glu15, Lys43, and Tyr46 may be important in the catalytic efficiency and thermostability of enzyme. The new structural and mechanistic insights obtained from computational study should be valuable for detailed structures and mechanisms of the member of the DJ-1 superfamily....
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PMID: 20711794
PDF is available here.
PDF is available here.
Abstract
The pleiotropic functions of disaccharide trehalose in the biology of nematodes and its absence from mammalian cells suggest that its biosynthesis may provide a useful target for developing novel nematicidal drugs. The trehalose-6-phosphate phosphatase (TPP), one of the enzymes of trehalose metabolis...
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PMID: 21658361
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)10 2011
Abstract
These results suggest that the active site region may be amongst the earliest parts of the structure to unfold. Detergent-solubilized Na,K-ATPases from the two sources show the similar differences in thermal stability as the membrane-bound species, but inactivation occurs at a lower temperature for...
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PMID: 21712026
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)9 2011
Abstract
We studied the effects of a chemically modified β-CD (BCD07056), on the aggregating and refolding properties of BlaPChBD, a hybrid protein obtained by inserting the chitin binding domain of the human macrophage chitotriosidase into the class A β-lactamase BlaP from Bacillus licheniformis 749/I dur...
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PMID: 21621654
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)9 2011
Abstract
We used biophysical and molecular modeling tools to assess structural properties of Staphylococcus xylosus lipase (SXL). We studied the thermal unfolding of this protein and its zinc-dependent thermotolerance. We demonstrated that SXL is able to be active and stable at moderate temperatures, but thi...
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PMID: 21621655
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)5 2011
Abstract
We present here the biochemical characterization of its activity in the aldol condensation reaction. The aldol condensation reaction parameters were determined using as acceptors both salicylaldehyde, which is the natural substrate taking part to the naphthalene degradative pathway, and benzaldehyde...
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PMID: 21443971
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)5 2011
Abstract
Enzymes from thermophiles are poorly active at temperatures at which their mesophilic homologs exhibit high activity and attain corresponding active states at high temperatures. In this study, comparative molecular dynamics (MD) simulations, supplemented by normal mode analysis, have been performed o...
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PMID: 21440684
PDF is available here.
PDF is available here.
Abstract
A novel gene (designated as tan410) encoding tannase was isolated from a cotton field metagenomic library by functional screening. Sequence analysis revealed that tan410 encoded a protein of 521 amino acids. SDS-PAGE and gel filtration chromatography analysis of purified tannase suggested that Tan410...
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PMID: 21388130
PDF is available here.
PDF is available here.
Abstract
Matrix metalloproteinase 8 (MMP-8) has been reported to have a key role in several pathologic conditions, like heart diseases, osteoarthritis, multiple sclerosis, and various other inflammatory conditions. Therefore, there is a great interest regarding the development of MMP-8 select...
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PMID: 21397574
PDF is available here.
PDF is available here.
Abstract
We characterized an active site variant (Glu473Gln) in which partitioning between aldehyde release versus carboligation is inverted with an up to 100-fold preference for the latter pathway. Due to a defective protonation of the central carbanion/enamine intermediate, substrate turnover stalls at thi...
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PMID: 21341803
PDF is available here.
PDF is available here.
Abstract
We report a simple procedure for immobilization of OPH on biocompatible gelatin pads. The covalent coupling of OPH using glutaraldehyde spacer has been found to dramatically improve the enzyme stability. There is no apparent loss of OPH activity in OPH-gelatin pads stored at room temperature for mor...
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PMID: 21469599
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)4 2011
Abstract
We found that RNase can be thermally unfolded at 65°C under non-reductive conditions while its native disulfide bonds remain intact, and the unfolded RNase can refold and reactivate during cooling. Co-incubation of RNase with PDI or PDIp during thermal unfolding can inactivate RNase in a PDI/PDIp c...
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PMID: 21238616
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)3 2011
Abstract
We developed a procedure that randomly mutagenizes a specific segment of a gene through error-prone PCR and screens for mutants on the basis of the properties of the holoenzymes reconstituted in vivo (REM-ivrs). Previous work showed that the N-terminal domain includes the valine-binding ACT domain o...
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PMID: 21224018
PDF is available here.
PDF is available here.
Abstract
Pyrazinamidase (PncA) activates the first-line antituberculous drug pyrazinamide into pyrazinoic acid. The crystal structure of the Mycobacterium tuberculosis PncA protein has been determined, showing significant differences in the substrate binding cavity when compared to the pyrazinamidases from Py...
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PMID: 21283666
PDF is available here.
PDF is available here.
Abstract
We show an unanticipated mechanism for inheritance of DNA methylation patterns where the epigenetic mark not only recruits the catalyzing enzyme but also regulates the protein level, i.e. the enzymatic product (5-methylcytosine) determines the level of the methylase, thus forming a novel homeostatic...
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PMID: 21304883
PDF is available here.
PDF is available here.
Abstract
Amylopullulanse (E. C. 3.2. 1. 1/41) has the enzymatic activities of both alpha-amylase (E. C. 3.2. 1. 1) and pullulanase (E. C. 3. 2. 1. 41), and is classified into the glycoside hydrolase 13 and 57 families. Amylopullulanse can hydrolyze both the alpha-1,4 and alpha-1,6 glucosidic bonds and is valu...
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PMID: 21465785
PDF is available here.
PDF is available here.
Abstract
We have developed a strategy for rapid and combinatorial optimization of the hot spot residues of enzymes. After combinatorial randomization of target locations in the Candida antarctica lipase B (CalB) gene, the individual variant genes isolated in the E.coli cells were expressed in the cell-free p...
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PMID: 21312354
PDF is available here.
PDF is available here.
Abstract
A strain of endophytic fungus EF6 isolated from Thai medicinal plants was found to produce higher levels of extracellular glucoamylase. This strain produced glucoamylase of culture filtrate when grown on 1% soluble starch. The enzyme was purified and characterized. Purification steps involved (NH4)2S...
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PMID: 21950121
PDF is available here.
PDF is available here.
Abstract
We have investigated the molecular basis for the action mode of xylanases 10B at high temperatures using biochemical, biophysical and crystallographic methods. The crystal structure of xylanase 10B from hyperthermophilic bacterium Thermotoga petrophila RKU-1 (TpXyl10B) has been solved in the native...
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PMID: 21070746
PDF is available here.
PDF is available here.
Bioresource Technology (101)22 2010
Abstract
I and MFX II, from the thermophilic fungus Malbranchea flava MTCC 4889 with molecular masses of 25.2 and 30kDa and pIs of 4.5 and 3.7, respectively were purified to homogeneity. The xylanases were optimally active at pH 9.0 and at 60 degrees C, exhibited a half-life of 4h at 60 degrees C, and showed...
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PMID: 20630749
PDF is available here.
PDF is available here.
Abstract
Streptomyces sp. 7b showed highest xylanase activity among 41 bacterial isolates screened under submerged fermentation. The organism grew over broad pH (5-11) and temperatures range (25-55 degrees C) and displayed maximum xylanase production on wheat bran (1230 U/g) under solid-state fermentation. X...
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PMID: 20419509
PDF is available here.
PDF is available here.
Abstract
A gene encoding methylglyoxal synthase from Thermus sp. GH5 (TMGS) was cloned, sequenced, overexpressed, and purified by Q-Sepharose. The TMGS gene was composed of 399 bp which encoded a polypeptide of 132 amino acids with a molecular mass of 14.3 kDa. The K (m) and k (cat) values of TMGS were 0.56...
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PMID: 20419481
PDF is available here.
PDF is available here.
Biochemical Journal (432)1 2010
Abstract
We provide evidence indicating that it is the 26S isoform of the proteasome that is responsible for intracellular degradation of the hTS polypeptide. In addition, we have used targeted in vitro mutagenesis to show that an Arg-Arg motif at residues 10-11 is required for proteolysis, an observation th...
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PMID: 20815815
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)43 2010
Abstract
We provide evidence for the ubiquitin ligase/proteosome pathway in regulating human Mps1 levels during late mitosis through G(1) phase. First, we showed that treatment of HEK 293T cells with the proteosome inhibitor MG132 resulted in an increase in both the polyubiquitination and the accumulation of...
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PMID: 20729194
PDF is available here.
PDF is available here.
Abstract
As³+ bound to the two-domain, recombinant human metallothionein (isoform 1a) is stable at pH 7 and translocates via protein-protein interactions to other metallothionein proteins. The data show As³+ transfer from the two-domain β-α-hMT to binding sites in the isolated apo-β-hMT and apo-α-hMT....
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PMID: 20833145
PDF is available here.
PDF is available here.
Bioresource Technology (101)19 2010
Abstract
The effects of alcohol treatment on the activity and loading amount of Candida rugosa lipase (CRL), Candida Antarctica lipase B (CALB) and Porcine Pancreas lipase (PPL) immobilized on methyl-modified silica aerogels were investigated, and the fluorescent analysis was used to explore...
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PMID: 20576560
PDF is available here.
PDF is available here.
Abstract
A string-like carbon fiber was found to be very suitable as a working electrode material for direct electrochemical oxidation of β-nicotinamide adenine dinucleotide reduced form (NADH), and direct use of it for an enzyme reactor was possible. The electrochemical NAD+ recycling system was applied to...
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PMID: 20834161
PDF is available here.
PDF is available here.
Abstract
Two different endophytic strains, ESRAA1997 and ALAA2000, were isolated from the Egyptian herbal plant Anastatica hierochuntica. The 2 strains produced alkaline serine protease and were identified based on their phenotypic and chemotypic characteristics as different strains of Micromonospora spp. Bo...
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PMID: 20921985
PDF is available here.
PDF is available here.
Abstract
β-D-Glucose-1-phosphate (βGlc1P) is an efficient glucosyl donor for both enzymatic and chemical glycosylation reactions but is currently very costly and not available in large amounts. This article provides an efficient production method of βGlc1P from trehalose and phosphate using the thermostab...
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PMID: 20799297
PDF is available here.
PDF is available here.
Abstract
Our results indicate that the OpdA Tyr(607) residue plays an important role in the enzyme-substrate interaction and in the hydrolytic activity.
2010 Elsevier Inc. All rights reserved....
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PMID: 20513640
PDF is available here.
PDF is available here.
Abstract
In a screening of saprotrophic, ectomycorrhizal and plant pathogen ascomycetes a frequent occurrence of laccase was observed. Lamprospora wrightii, the best producing organism, was chosen to elucidate the properties of a laccase from a moss-associated, saprotrophic ascomycete. The ex...
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PMID: 20652905
PDF is available here.
PDF is available here.
Biotechnology Letters (32)8 2010
Abstract
A thermophilic esterase, SsoPEst, from Sulfolobus solfataricus P2 was cloned and expressed in E. coli AD494 (DE3). Gene sequencing indicated the encoded 353 amino acids had less than 32% identity with reported esterases. The recombinant enzyme hydrolyzed p-nitrophenyl esters but not tributyrin or tr...
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PMID: 20386955
PDF is available here.
PDF is available here.
Abstract
We analysed the secondary structure and structural organization of a purified 65kDa N-domain ACE (nACE) from Wistar rat mesangial cells, a 90 kDa nACE from spontaneously hypertensive rats and a 130 kDa somatic ACE. The C-terminal alignment of the 65 kDa nACE with rat ACE revealed that the former was...
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PMID: 20435057
PDF is available here.
PDF is available here.
Abstract
Amylases are the most important hydrolytic enzymes for starch-based industries. It is desirable that alpha-amylases should be active at high temperature of gelatinization (100-110 degrees C) and liquefaction (80-90 degrees C) to economize processes. Therefore, thermostable and thermoactive enzyme fr...
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PMID: 20417228
PDF is available here.
PDF is available here.
Abstract
More than 150 different point mutations in POLG, the gene encoding the human mitochondrial DNA polymerase gamma (pol gamma), cause a broad spectrum of childhood and adult onset diseases like Alpers syndrome, ataxia-neuropathy syndrome and progressive external ophthalmoplegia. These disease mutations...
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PMID: 20176107
PDF is available here.
PDF is available here.
Abstract
We identified a novel GST gene of Taenia solium metacestodes (TsMs), which is a causative agent of neurocysticercosis. The 804 bp-long cDNA encoded a 639 bp open reading frame (212 amino acid polypeptide), which exhibited the structural motif and domain organisation characteristic of GST. It formed...
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PMID: 20385139
PDF is available here.
PDF is available here.
Bioresource Technology (101)15 2010
Abstract
A novel dimeric 58 kDa keratinase is reported from Bacillus licheniformis ER-15. The bacterium produced 244 U/ml keratinase in 48 h which was increased by eight fold (1962 U/ml) after medium optimization by one-variable-at-a-time and response surface methodology. Enzyme was concentrated by ultrafilt...
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PMID: 20347294
PDF is available here.
PDF is available here.
Bioresource Technology (101)15 2010
Abstract
Homological series of 14 achiral derivates and series of five chiral derivates of imidazole were tested in vitro as inhibitors of hydrolysis of acetylcholine using enzyme preparation of acetylcholinesterase from electric eel. The batch stirred reactor at 25 degrees C, pH 8 (phosphate buffer), ionic...
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PMID: 20335028
PDF is available here.
PDF is available here.
Bioresource Technology (101)15 2010
Abstract
Plant is one of the important resources for glycosyl hydrolase production. A beta-exoglucanase with molecular weight of 63.1 kDa was purified from fresh maize stover and subjected to enzymatic characterization. The optimal temperature and pH of the beta-exoglucanase was 40 degrees C and 6.0, respect...
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PMID: 20304632
PDF is available here.
PDF is available here.
Abstract
A novel keratinase from Chryseobacterium sp. strain kr6 was purified to homogeneity by (NH(4))(2)SO(4) precipitation, gel permeation on Sephadex G-100, and Q-Sepharose Fast Flow anion-exchange chromatography. The molecular weight of the purified enzyme was around 20 kDa. Kinetic and thermodynamic pa...
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PMID: 19936635
PDF is available here.
PDF is available here.
Abstract
Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enz...
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PMID: 19688300
PDF is available here.
PDF is available here.
Abstract
A new white-rot fungus SYBC-L1, which could produce an extracellular laccase, was isolated from a decayed Elaeocarpus sylvestris. The strain was identified as Pycnoporus sp. SYBC-L1 according to the morphological characteristics and ribosomal ITS1-5.8S-ITS2 RNA genomic sequence analysis. The highest...
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PMID: 19842067
PDF is available here.
PDF is available here.
Abstract
Dialdehyde starch (DAS) was used as a novel coupling agent to prepare chitosan carrier to immobilize the xylanase from Aspergillus niger A-25. Compared with glutaraldehyde-cross-linked chitosan (CS-GA) and pure chitosan beads, the DAS-cross-linked chitosan (CS-DAS) beads exhibited the highest xylana...
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PMID: 19823778
PDF is available here.
PDF is available here.
Abstract
In this study, Mirabilis jalapa tuber powder (MJTP) was used as a new complex organic substrate for the growth and production of fibrinolytic enzymes by a newly isolated Bacillus amyloliquefaciens An6. Maximum protease activity (1,057 U/ml) with casein as a substrate was obtained when the strain was...
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PMID: 19842068
PDF is available here.
PDF is available here.
Abstract
Successful industrial use of amylases requires that they are sufficiently stable and active at application conditions, e.g., at high temperature in starch-liquefaction process. In the present study, site-directed mutagenesis was used to enhance the thermal stability and calcium independency of a mes...
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PMID: 20177823
PDF is available here.
PDF is available here.
Abstract
A low molecular weight endo-xylanase (EC 3.2.1.8) was purified from an edible mushroom Termitomyces clypeatus grown in submerged medium with oat spelt xylan. Xylanase was purified to apparent homogeneity by ammonium sulfate fractionation and gel filtration chromatography. Its molecular weight was de...
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PMID: 19728166
PDF is available here.
PDF is available here.
Abstract
I and II, respectively. The native enzymes' molecular masses of 23.8 and 30.8 kDa were estimated for xylanase I and II, respectively, by molecular exclusion chromatography. Both enzymes are glycoproteins with optimum temperature and pH of 50 degrees C and pH 2.5 for xylanase I and 55 degrees C and p...
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PMID: 19680819
PDF is available here.
PDF is available here.
Abstract
These results suggest that this keratinase may be a useful alternative and ecofriendly route for handling the abundant amount of waste feathers and for applications in detergent formulations....
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PMID: 20012915
PDF is available here.
PDF is available here.
Abstract
We investigated the effect of different polyol compounds, e.g. glycerol, mannitol and PEG-6000 on the in vitro stability of purified hPAH produced in a heterologous prokaryotic expression system. The recombinant human enzyme was stored in the presence of the studied stabilizing agents at different t...
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PMID: 19937396
PDF is available here.
PDF is available here.
Abstract
Abstract
We investigate the mechanism of cathepsin K action at physiological plasma pH and its regulation by modifiers that bind outside of the active site. We show that at physiological plasma pH the enzyme fluctuates between multiple conformations that are differently susceptible to macromolecular inhibito...
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PMID: 20450492
PDF is available here.
PDF is available here.
Abstract
A family 5 glycoside hydrolase from Clostridium phytofermentans was cloned and engineered through a cellulase cell surface display system in Escherichia coli. The presence of cell surface anchoring, a cellulose binding module, or a His tag greatly influenced the activities of wild-type and mutant en...
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PMID: 20511418
PDF is available here.
PDF is available here.
Abstract
With emphasis on thermal behavior in presence of different pH conditions and salts, the kinetic and thermodynamic parameters of purified polygalacturonase (PG) of E. carotovora subsp. carotovora (Ecc) BR1 were studied since characterization of an enzyme is significant in the context of burgeoning bi...
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PMID: 20668400
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We examined properties of a series of 27 mutant variants, which together represent 70% of known alleles observed in patients with homocystinuria due to CBS deficiency. The median amount of SDS-soluble mutant CBS polypeptides in the pellet after centrifugation of bacterial extracts was increased by 5...
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PMID: 20506325
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We study with this system the effects of nine clinical mutations and one polymorphism on CPS1 solubility, stability, activity, and kinetic parameters for NAG. Five of the mutations (p.T471N, p.Q678P, p.P774L, p.R1453Q, and p.R1453W) are first reported here, in three severe CPS1D patients. p.P774L, p...
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PMID: 20578160
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We study with this system the effects of nine clinical mutations and one polymorphism on CPS1 solubility, stability, activity, and kinetic parameters for NAG. Five of the mutations (p.T471N, p.Q678P, p.P774L, p.R1453Q, and p.R1453W) are first reported here, in three severe CPS1D patients. p.P774L, p...
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PMID: 20578160
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We study with this system the effects of nine clinical mutations and one polymorphism on CPS1 solubility, stability, activity, and kinetic parameters for NAG. Five of the mutations (p.T471N, p.Q678P, p.P774L, p.R1453Q, and p.R1453W) are first reported here, in three severe CPS1D patients. p.P774L, p...
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PMID: 20578160
PDF is available here.
PDF is available here.