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Polyhydroxyalkanoates (0):
Recent article
Polyhydroxyalkanoates
Biomacromolecules (12)9 2011
Abstract
Polyhydroxyalkanoates (PHA) synthesis genes phbC and orfZ cloned from Ralstonia eutropha H16 were transformed into beta-oxidation weakened Pseudomonas putida KTOY08ΔGC, a mutant of P. putida KT2442. The recombinant P. putida strain termed KTHH06 was able to produce a short-chain-len...
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PMID: 21863836
PDF is available here.
PDF is available here.
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Abstract
PHAs are biodegradable and environmentally friendly thermoplastics. The major contributor to PHA production cost is carbon substrate cost, therefore it is desirable to produce PHA from waste/byproducts like Jatropha biodiesel byproducts. This study was done using Jatropha biodiesel byproduct as carb...
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PMID: 20417229
PDF is available here.
PDF is available here.
Bioresource Technology (101)15 2010
Abstract
Polyhydroxyalkanoate synthase gene phaC(ah) in Aeromonas hydrophila strain 4AK4 was deleted and its function was replaced by phaC1(ps) cloned from Pseudomonas stutzeri strain 1317 which favors 3-hydroxyhexanoate (3HHx) and longer chain length monomers. Genes fadD and fadL encoding Escherichia coli a...
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PMID: 20236821
PDF is available here.
PDF is available here.
Abstract
Polyhydroxyalkanoate (PHA) from one fermentation process shows diverse physical properties when extracted using different methods. Pseudomonas aeruginosa strain has been previously isolated from the Egyptian ecosystem was cultivated on olive oil as a carbon source under PHA accumulation conditions....
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PMID: 20566449
PDF is available here.
PDF is available here.
Nanotechnology (21)20 2010
Abstract
We have explored the biofabrication process of bacterial inclusion bodies, particulate proteinaceous materials (ranging from 50 to 500 nm in diameter) recently recognized as suitable for surface topographical modification and tissue engineering. Inclusion bodies have been widely described as spheric...
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PMID: 20413834
PDF is available here.
PDF is available here.
Abstract
High substrate costs decrease the profitability of polyhydroxyalkanoates (PHAs) production, and thus low-cost carbon substrates coming from agricultural and industrial residuals are tested for the production of these biopolymers. Among them crude glycerol, formed as a byproduct during biodiesel prod...
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PMID: 20519907
PDF is available here.
PDF is available here.
Abstract
The nucleotide composition of key enzymes involved in medium-chain-length polyhydroxyalkanoates (mcl-PHA) synthesis was analyzed in two newly isolated strains of Pseudomonas. The isolated strains were tested for their abilities to synthesize polyhydroxyalkanoates using three different substrates as...
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PMID: 20204456
PDF is available here.
PDF is available here.
Abstract
We compare native granules and artificial granules made from market poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-hydroxyvalerate), P(3HB-co-3HV), for their ability as sizing agent. Paper sizing was assayed by measuring the resistance of sized paper to penetration by aqueous fluids. Ou...
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PMID: 20155934
PDF is available here.
PDF is available here.
Abstract
Abstract
Azotobacter chroococcum H23 (CECT 4435), Azotobacter vinelandii UWD, and Azotobacter vinelandii (ATCC 12837), members of the family Pseudomonadaceae, were used to evaluate their capacity to grow and accumulate polyhydroxyalkanoates (PHAs) using two-phase olive mill wastewater (TPOMW, alpeorujo) dilu...
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PMID: 20372033
PDF is available here.
PDF is available here.
Abstract
Withania somnifera L. has been traditionally used as a sedative and hypnotic. The present study was carried out for the purification, characterization, and in vitro cytotoxicity of L-asparaginase from W. somnifera L. L-Asparaginase was purified from the fruits of W. somnifera L. up t...
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PMID: 19448978
PDF is available here.
PDF is available here.
Abstract
Polyhydroxyalkanoates (PHAs) serve numerous bacteria as storage compounds. It is generally believed that under unbalanced growth conditions, n-hydroxyalkanoates are synthesized inside the bacterial cells, polymerized to polyesters, and densely packed in granules. In the absence of extracellular carb...
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PMID: 20036809
PDF is available here.
PDF is available here.
Abstract
The effects of phosphorus and nitrogen limitation on polyhydroxyalkanoate (PHA) production and accumulation by activated sludge biomass with acetate as a carbon source were investigated. Pre-selected influent carbon-phosphorus (C:P, W/W) of 100, 160, 250, 500 and 750, and carbon-nitrogen (C:N, W/W)...
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PMID: 21235192
PDF is available here.
PDF is available here.
Abstract
We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) sy...
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PMID: 19933347
PDF is available here.
PDF is available here.
Abstract
The present study was conducted to evaluate the specific acetate uptake rates of microorganisms with and without polyhydroxyalkanoates (PHA) accumulation. Activated sludge was aerobically incubated with 75 mgC L(-1) radiolabeled or non-labeled acetate, and acetate consumption and PHA accumulation we...
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PMID: 20595747
PDF is available here.
PDF is available here.
Abstract
Polyhydroxybutyrate (PHB), co-polyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), and polylactic acid (PLA) were used to prepare nanoparticles with average sizes of 160, 250 and 150 nm, respectively. A lipid-soluble colorant, rhodamine B isothiocyanate (RBITC), was employed to study dru...
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PMID: 20040158
PDF is available here.
PDF is available here.
Abstract
Polyhydroxyalkanoates (PHAs) are a newer family of biomaterials for tissue-engineering applications. The objective of this study is to investigate the behavior of human mesenchymal stem cells (hMSCs) grown on various PHA films. The surface characteristics of PHA co-polymer films were varied by the c...
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PMID: 20040151
PDF is available here.
PDF is available here.
BMC Microbiology (10) 2010
Abstract
We followed the enzymatic activities of PhaC and PhaZ during growth and correlated them to the amount of phasins on the PHA granules. It was found that in P. putida PhaC and PhaZ are concomitantly active, resulting in parallel synthesis and degradation of PHA. Moreover PhaC activity was found to be...
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PMID: 20937103
PDF is available here.
PDF is available here.
Abstract
We have explored the feasibility of using M. extorquens for producing functionalized PHAs containing C-C double bonds, thus, making them amenable to future chemical/biochemical modifications for high value applications.
Our proprietary M. extorquens ATCC 55366 was found unable to yield functionalize...
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PMID: 20846434
PDF is available here.
PDF is available here.
Biotechnology Letters (31)10 2009
Abstract
We further showed that the recombinant strain grown in kanamycin-containing culture medium synthesized a blend of PHA that also contains a high content of 3-hydroxyoctanoate and 3-hydroxydecanoate as its repeat-unit monomers. Genomic analysis suggested the existence of two PHA synthase genes in R. e...
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PMID: 19557308
PDF is available here.
PDF is available here.
Abstract
We disclose that there are more actinobacteria PHA producers in addition to the genera cited. Some unusual genera, such as Streptoalloteichus, and some genera frequently present in soil, such as Streptacidiphilus, have been found. Thirty-four isolates were able to accumulate poly(3-hydroxybutyrate)...
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PMID: 19767851
PDF is available here.
PDF is available here.
Abstract
Polyhydroxyalkanoates (PHAs) are attractive biomaterials in both conventional medical devices and tissue engineering. PHA synthase is responsible for catalyzing the formation of Polyhydroxyalkanoates (PHA), but its structural information is limited. Hence, this study focuses to predict 3D model for...
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PMID: 19385705
PDF is available here.
PDF is available here.
Journal of Bacteriology (191)10 2009
Abstract
FEMS Microbiology Letters (292)2 2009
Abstract
I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine...
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PMID: 19187205
PDF is available here.
PDF is available here.
Abstract
Studies conducted with various inexpensive carbon sources such as whey, vegetable oils (palm, mustard, soybean and coconut), a low-cost source of glucose-D, rice and wheat bran, and mustard and palm oil cakes demonstrated palm oil as the best substrate for accumulation of a novel short-chain-length-...
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PMID: 19052786
PDF is available here.
PDF is available here.
Abstract
Ultra-high molecular weight polyhydroxyalkanoates (PHAs) with low polydispersity index (PDI = 1.3) were produced in a novel, pilot scale application of mixed cultures of nitrogen-fixing bacteria. The number average molecular weight (M (n)) of the poly(3-hydroxybutyrate) (P(3HB)) and poly(3-hydroxybu...
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PMID: 19125246
PDF is available here.
PDF is available here.
Abstract
Unsaturated medium-chain-length polyhydroxyalkanoates (MCL-PHA) were produced at a productivity of 0.63-1.09 g PHA l(-1) h(-1) with final PHA content ranging from 42.6 to 55.8% in single-stage, carbon-limited, fed-batch fermentations of Pseudomonas putida KT2440. A mixture of nonanoic acid (NA) and...
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PMID: 19050862
PDF is available here.
PDF is available here.
Abstract
Organic acids are considered as potential substrates for biosynthesis of polyhydroxyalkaonates. The acids may also be the metabolic inhibitors at moderate concentration levels. In this study, Ralstonia eutropha was used to elucidate the protein regulations when the bacterial cells pre-cultivated on...
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PMID: 19270755
PDF is available here.
PDF is available here.
Abstract
Poly (hydroxyalkanoates) (PHAs) have recently attracted a great deal of academic and industrial interest for their biodegradability and biocompatibility making them suitable for environmental and biomedical applications. Poly(3-hydroxybutyrate-) (PHB-) and Poly(DL-lactide-co-glycolide) (PLGA-) based...
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PMID: 19789653
PDF is available here.
PDF is available here.
Abstract
Conspicuous cytoplasmic granules are reported in a magnetotactic multicellular prokaryote named 'Candidatus Magnetoglobus multicellularis'. Unfortunately, this microorganism, which consists of an assembly of gram-negative bacterial cells, cannot yet be cultivated, limiting the biochemical analysis o...
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PMID: 18599298
PDF is available here.
PDF is available here.
Abstract
Production of polyhydroxyalkanoates (PHA) by mixed cultures has been widely studied in the last decade. Storage of PHA by mixed microbial cultures occurs under transient conditions of carbon or oxygen availability, known respectively as aerobic dynamic feeding and anaerobic/aerobic process. In these...
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PMID: 19002455
PDF is available here.
PDF is available here.
Bioresource Technology (99)17 2008
Abstract
Cellulosic biomass is a potentially inexpensive renewable feedstock for the biorefineries of fuels, chemicals and materials. Sugarcane bagasse was pretreated in dilute acid solution under moderately severe conditions, releasing sugars and other hydrolysates including volatile organic acids, furfural...
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PMID: 18474421
PDF is available here.
PDF is available here.
Abstract
We conclude that GAO do not have metabolic advantages over PAO concerning the effects of temperature on their aerobic metabolism, and competitive advantages are due to anaerobic processes....
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PMID: 18623226
PDF is available here.
PDF is available here.
Bioresource Technology (99)15 2008
Abstract
The combination of plant oils and 3-hydroxyvalerate (3HV) precursors were evaluated for the biosynthesis of polyhydroxyalkanoate (PHA) copolymers containing 3HV monomers by Cupriavidus necator H16. Among various mixtures of plant oils and 3HV-precursors, the mixture of palm kernel oil and sodium pro...
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PMID: 18325764
PDF is available here.
PDF is available here.
Biomaterials (29)27 2008
Abstract
Polyhydroxyalkanoates (PHAs) have been demonstrated to be a family of biopolymers with good biodegradability and biocompatibility. To mimic the real microenvironment of extracellular matrix (ECM) for cell growth, novel nanofiber matrices based on PHA polymers were prepared via a phase separation pro...
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PMID: 18585779
PDF is available here.
PDF is available here.
Environmental Science (29)9 2008
Abstract
To reduce the production cost of polyhydroxyalkanoates (PHAs), the process feasibility and physicochemical properties of PHAs synthesized by Alcaligenes latus, Staphylococcus epidermidis and activated sludge from malt waste, soy waste, confectionary waste, ice cream waste, milk waste, sesame oil and...
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PMID: 19068658
PDF is available here.
PDF is available here.
Environmental Science (29)9 2008
Abstract
With the long-term cultured enhanced biological phosphorus removal systems in sequencing batch reactors, the effect of different ratios of propionic acid to acetic acid on the transformations of phosphorus and polyhydoxyalkanoates (PHA), and the metabolism stoichiometry of PHA by phosphorus accumula...
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PMID: 19068641
PDF is available here.
PDF is available here.
Abstract
We report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers--polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenz...
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PMID: 18629491
PDF is available here.
PDF is available here.
Abstract
Pseudomonas guezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was...
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PMID: 18538385
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (94)2 2008
Abstract
Expression of Pseudomonas aeruginosa genes PHA synthase1 (phaC1) and (R)-specific enoyl CoA hydratase1 (phaJ1) under a lacZ promoter was able to support production of a copolymer of Polyhydroxybutyrate (PHB) and medium chain length polyhydoxyalkanoates (mcl-PHA) in Escherichia coli. In order to impr...
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PMID: 18357511
PDF is available here.
PDF is available here.
Abstract
We believe to be the first time, that a single organism can degrade insoluble cellulose and under similar conditions can produce and accumulate PHA. Additional work is necessary to more fully characterize these capabilities and to optimize the PHA production and purification.
(c) 2008 Wiley Periodic...
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PMID: 18383127
PDF is available here.
PDF is available here.
Abstract
One of the options enabling more economic production of polyhydroxyalkanoates compared to pure cultures is the application of mixed cultures. The use of a microbial community in a sequencing batch reactor has a few advantages: a simple process control, no necessity for sterile processing, and possib...
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PMID: 18418634
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (284)2 2008
The alternative sigma factor, sigmaS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida.
Abstract
These results suggest that sigma(S) might control the genes involved in polyhydroxyalkanoate metabolism, possibly in an indirect manner. In addition, survival and oxidative stress assays performed under polyhydroxyalkanoate- and nonpolyhydroxyalkanoate- accumulating conditions demonstrated that the...
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PMID: 18498401
PDF is available here.
PDF is available here.
Bioresource Technology (99)11 2008
Abstract
Medium chain length polyhydroxyalkanoates (mcl-PHA) and different rubbers; namely natural rubber, nitrile rubber and butadiene rubber were blended at room temperature using solution blending technique. Blends constituted 5%, 10% and 15% of mcl-PHA in different rubbers. Thermogravimetric analysis of...
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PMID: 17764931
PDF is available here.
PDF is available here.
Bioresource Technology (99)11 2008
Abstract
Whey permeate from dairy industry was hydrolyzed enzymatically to cleave its main carbon source, lactose, to glucose and galactose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Pseudomonas hydrogenovora. In shaking flask experiments,...
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PMID: 18053709
PDF is available here.
PDF is available here.
Abstract
phaJ and fabG genes encoding (R)-specific 2-enoyl-CoA hydratase and 3-ketoacyl-acyl carrier protein reductase, respectively, were cloned from Pseudomonas putida KCTC1639 and amplified in the parent strain to achieve oversupplementation of (R)-3-hydroxyalkanoate monomer units for the biosynthesis of...
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PMID: 18691538
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (283)2 2008
Abstract
Monomers of microbial polyhydroxyalkanoates, mainly 3-hydroxyhexanoic acid (3HHx) and 3-hydroxyoctanoic acid (3HO), were produced by overexpressing polyhydroxyalkanoates depolymerase gene phaZ, together with putative long-chain fatty acid transport protein fadL of Pseudomonas putida KT2442 and acyl-...
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PMID: 18422622
PDF is available here.
PDF is available here.
Abstract
We developed a new method for the reductive depolymerization of medium chain-length PHAs, leading to monomeric diols that can be separated and quantified by HPLC/MS. Reduction is performed at room temperature with lithium aluminum hydride within 5-15 min. The new method is faster and simpler than th...
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PMID: 18461645
PDF is available here.
PDF is available here.
Abstract
We concluded that ACS1 is located on the PHA granules and may play a central role in mobilization of PHA, for example, conversion of hydroxycarboxylic acid monomers to hydroxycarboxyl-CoA, which can be further utilized by the cells....
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PMID: 18471011
PDF is available here.
PDF is available here.
Abstract
The marine bacterium Saccharophagus degradans was investigated for the synthesis of polyhydroxyalkanoates (PHAs), using glucose as the sole source of carbon in a two-step batch culture. In the first step the microorganism grew under nutrient balanced conditions; in the second step the cells were cul...
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PMID: 18193466
PDF is available here.
PDF is available here.
Abstract
The PHA yields obtained were found to be 13.4, 38, 13.15 and 33.33% dcw for potassium, nitrogen, sulphur and phosphorus limitations, respectively. Gas chromatography-mass spectrometry analysis of the isolated polymers showed the presence of P(3HB) under nitrogen, sulphur and phosphate-limiting condi...
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PMID: 18194257
PDF is available here.
PDF is available here.
Plant Journal (54)4 2008
Abstract
We focus on plant biopolymers that are used as materials in bulk applications, such as plastics and elastomers, in the context of depleting resources and climate change, and consider technical and scientific bottlenecks in the production of novel or improved materials in transgenic or alternative cr...
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PMID: 18476872
PDF is available here.
PDF is available here.
Abstract
The bacterium H. profundus produces PHA of 3HB and 3HV monomers from different carbon sources. SIGNIFICANCE AND IMPACT OF THE STUDY: PHAs share physical and material properties that suggest them for application in various areas, and are considered as an alternative to nonbiodegradable plastics produ...
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PMID: 18179545
PDF is available here.
PDF is available here.
Abstract
We developed a stress-induced system by which the PHB biosynthesis pathways can be induced under stress conditions. Fermentation results showed that recombinant E. coli DH5alpha (pQKZ103) harboring this system was able to accumulate polyhydroxybutyrate up to 85.8% of cell dry weight in minimal gluco...
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PMID: 18347791
PDF is available here.
PDF is available here.
Abstract
The characterization of polyhydroxyalkanoates (PHA) produced by mixed cultures is fundamental for foreseeing the possible final applications of the polymer. In this study PHA produced under aerobic dynamic feeding (ADF) conditions are characterized. The PHA produced shows a stable average molecular...
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PMID: 18157852
PDF is available here.
PDF is available here.
Abstract
Polyhydroxyalkanoate (PHA) production was achieved using tomato cannery waste coupled with a mixed microbial culture during wastewater treatment. The two-stage PHA production process comprised a sequencing batch reactor (SBR), operating under a periodic feast-famine regime, to accomplish simultaneou...
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PMID: 18536488
PDF is available here.
PDF is available here.
Biomaterials (29)10 2008
Abstract
Polyhydroxyalkanoate (PHA) copolymers of poly[(R)-3-hydroxybutyrate-co-5mol%-(R)-3-hydroxyhexanoate], poly[(R)-3-hydroxybutyrate-co-7mol%-4-hydroxybutyrate] and poly[(R)-3-hydroxybutyrate-co-97mol%-4-hydroxybutyrate] were electrospun to fabricate scaffolds with enhanced biocompatibility and bioabsor...
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PMID: 18155139
PDF is available here.
PDF is available here.
Abstract
Direct utilization of palm oil for the simultaneous production of polyhydroxyalkanoates (PHAs) and rhamnolipids was demonstrated using Pseudomonas aeruginosa IFO3924. By secreted lipase, palm oil was hydrolyzed into glycerol and fatty acids. Fatty acids became favorable carbon sources for cell growt...
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PMID: 18299827
PDF is available here.
PDF is available here.
Journal of Biotechnology (134)1-2 2008
Abstract
Polyhydroxyalkanoate (PHA) copolymers comprising the four monomers 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 3-hydroxy-2-methylvalerate (3HMV) and 3-hydroxy-2-methylbutyrate (3HMB) were generated using the recently discovered Defluviicoccus vanus-related glycogen accumulating organisms (DvGA...
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PMID: 18313162
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (93)3 2008
Abstract
We have demonstrated, for the first time, the polymerization of hydroxy butyryl CoA monomers in recombinant E. coli by PhaC1 of P. aeruginosa....
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PMID: 17906975
PDF is available here.
PDF is available here.