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Antisense Elements (Genetics) (3):
Recent article
Antisense Elements (Genetics)
Anticancer Research (30)6 2010
Abstract
We investigated the role of the EGFR ligand amphiregulin (AR) in modulating EGFR activation. For this, transformed epithelial mammary tumor cells NS2T2A1 were used in which AR or EGFR expression was down-regulated by antisense cDNA technique. This down-regulation was associated with a significant in...
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PMID: 20651357
PDF is available here.
PDF is available here.
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Cancer Letters (291)1 2010
Abstract
We report a novel E1A-mutant adenovirus (M6) with antisense HPV16 E6 E7 DNA inserted into the deleted 6.7K/gp19K region of E3. The target effects of M6 on HPV16-positive cervical cancer cells were evaluated in vivo and in vitro. By using cytopathic effect (CPE) and viral replication assays, we verif...
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PMID: 19903581
PDF is available here.
PDF is available here.
Abstract
The aim of this review is to provide the reader with an overview on the potential of dendritic polymers in the antisense delivery technology. Special emphasis has been placed on the different types of dendritic structures that have been reported and the modifications performed to increase their effi...
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PMID: 20380642
PDF is available here.
PDF is available here.
Hepato-gastroenterology (57)101 2010
Abstract
We examined the effects of antisense pEGFP-C1-mTOR on cell proliferation, apoptosis and migration of human hepatocellular carcinoma cell lines, HepG2 and SMMC7721 in vitro. We also observed the expression of mTOR and Akt by using RT-PCR and western blot. Our results showed that treatment of cells wi...
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PMID: 21033251
PDF is available here.
PDF is available here.
Abstract
Short nucleic acids targeting biologically important RNAs and plasmids have been shown to be promising future therapeutics; however, their hydrophilic nature greatly limits their utility in clinics and therefore efficient delivery vectors are greatly needed. Cell-penetrating peptides (CPPs) are rela...
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PMID: 19831582
PDF is available here.
PDF is available here.
Abstract
We designed, synthesized, and tested a novel molecular probe, in which the antisense oligodeoxynucleotide (ASODN) was labeled with superparamagnetic iron oxide (SPIO), and its efficiency was examined by in vitro MR imaging after SK-Br-3 mammary carcinoma cell lines (oncocytes) transfection. The SPIO...
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PMID: 19337837
PDF is available here.
PDF is available here.
Birth Defects Research (87)1 2009
Abstract
Spermatogenesis is a highly orchestrated developmental process by which spermatogonia develop into mature spermatozoa. This process involves many testis- or male germ cell-specific gene products whose expressions are strictly regulated. In the past decade the advent of high-throughpu...
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PMID: 19306351
PDF is available here.
PDF is available here.
BMC Genomics (10)1 2009
Abstract
By using alternative target preparation schemes, both the sense- and antisense-strand derived transcripts were labeled and hybridized to the Wheat GeneChip. Quality assurance verified that successful hybridization did occur in the antisense-strand assay. A stringent threshold for positive hybridizat...
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PMID: 19480707
PDF is available here.
PDF is available here.
BMC Genomics (10)1 2009
Abstract
First, we designed microarray probes to target the complementary strand of genes for which an antisense counterpart had been identified only in human public cDNA sources, but not in the mouse. We observed a prominent expression signal from 66.1% of 635 target genes, and 58 genes of these showed tiss...
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PMID: 19698135
PDF is available here.
PDF is available here.
Abstract
Different protein expression patterns of endometrial receptors were observed between the tested endometrial cell lines. The expression levels of PRA ratio to PRB, and the 50 kDa c-MET isoform were significantly lower in HEC-1A as compared with RL95-2. Attachment rates and growth of JAR spheroids int...
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PMID: 19220894
PDF is available here.
PDF is available here.
BMC Genomics (10)1 2009
Abstract
The expression of more than 2500 antisense transcripts were detected in normal breast duct luminal cells and in primary breast tumors substantially enriched for their epithelial cell content by DSA microarray. Expression of 431 NATs were confirmed by either of the other two technologies. A correspon...
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PMID: 19615061
PDF is available here.
PDF is available here.
Abstract
We transferred the regulatory sequences into the respectively heterologous systems, i.e. the Nin88 promoter into Arabidopsis and the AtcwINV2 promoter into tobacco. The specificities of both promoters are maintained in the heterologous backgrounds, but their activities are strongly reduced as GUS-st...
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PMID: 18825361
PDF is available here.
PDF is available here.
Abstract
Are plasmids selfish parasitic DNA molecules or an integrated part of the bacterial genome? This chapter reviews the current understanding of the persistence mechanisms of conjugative plasmids harbored by bacterial cells and populations. The diversity and intricacy of mechanisms affecting the succes...
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PMID: 19271180
PDF is available here.
PDF is available here.
Cancer Science (99)12 2008
Abstract
Vascular endothelial growth factor (VEGF) is an important regulator of angiogenesis, vasculogenesis and vascular permeability. Edema in glioma tumors is considered one of the most pathological characteristics, but the mechanism of regulating vascular permeability is still unclear. In the present stu...
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PMID: 19032372
PDF is available here.
PDF is available here.
Plant Journal (56)4 2008
Abstract
We came to realize that there is a significant amount of untapped potential in the publicly available T-DNA insertion lines. In addition to the GABI-Kat lines, which were designed specifically for activation tagging, lines from the SAIL and FLAGdb collections are also useful for this purpose. As wel...
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PMID: 18644000
PDF is available here.
PDF is available here.
Oncology Reports (20)4 2008
Abstract
We identified that 37 out of the 221 members of the antisense library exerted a marked inhibitory effect on the growth of Huh-7. The putative functional categorization of each gene was then conducted on the basis of the sequence information. The relative expression levels of target genes were measur...
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PMID: 18813834
PDF is available here.
PDF is available here.
Abstract
We hypothesized that if fatty acid alterations are a result of CFTR dysfunction, those alterations should be demonstrable in CF cell culture models. Two CF airway epithelial cell lines were used: 16HBE, sense and antisense CFTR cells, and C38/IB3-1 cells. Wild-type (WT) and CF cells were cultured in...
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PMID: 18441018
PDF is available here.
PDF is available here.
Abstract
Ribosomal protein S4 (RPSD), a part of the ribosomal small subunit, is one of the proteins that is a part of the ribosomal machinery and is a potential new target for the discovery of antibacterial agents. Continued screening of microbial extracts using antisense-sensitized rpsD Staphylococcus aureu...
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PMID: 18570471
PDF is available here.
PDF is available here.
Plant Physiology (147)1 2008
Abstract
Superoxide dismutases (SODs) are key components of the plant antioxidant defense system. While plastidic and cytosolic isoforms have been extensively studied, the importance of mitochondrial SOD at a cellular and whole-plant level has not been established. To address this, transgenic Arabidopsis (Ar...
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PMID: 18337490
PDF is available here.
PDF is available here.
Oncology Reports (19)4 2008
Abstract
We studied the expression of rTSbeta, a reverse orientation gene of TS, as a 5-FU resistance marker in patients with primary breast cancer. Expression of rTSbeta was examined in 129 patients with newly diagnosed breast cancer and five breast cancer cell lines by immunohistochemistry, immunocytochemi...
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PMID: 18357371
PDF is available here.
PDF is available here.
Abstract
APeg3 is an antisense transcript gene of Peg3, which has been recently identified from rat brain. Careful analyses of EST databases indicated that a homologous transcript also exists in other mammalian species, including mouse, cow and human. 5'-and 3'-RACE experiments have subsequently identified a...
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PMID: 18166281
PDF is available here.
PDF is available here.
Plant Physiology (146)2 2008
Abstract
We contrasted the stomatal red light response of wild-type tobacco (Nicotiana tabacum 'W38') with that of plants impaired in photosynthesis by antisense reductions in the content of either cytochrome b(6)f complex (anti-b/f plants) or Rubisco (anti-SSU plants). Both transgenic genotypes showed a low...
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PMID: 18065555
PDF is available here.
PDF is available here.
Transgenic Research (17)1 2008
Abstract
Pyrophosphate: fructose 6-phosphate 1-phosphotransferase (PFP) activity was successfully down-regulated in sugarcane using constitutively expressed antisense and untranslatable forms of the sugarcane PFP-beta gene. In young internodal tissue activity was reduced by up to 70% while no residual activi...
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PMID: 17431807
PDF is available here.
PDF is available here.
Abstract
We demonstrate that DNA or RNA templates can orchestrate these genome rearrangements in Oxytricha, supporting an epigenetic model for sequence-dependent comparison between germline and somatic genomes. A complete RNA cache of the maternal somatic genome may be available at a specific stage during de...
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PMID: 18046331
PDF is available here.
PDF is available here.
Abstract
We designed an intracellularly biodegradable triphenylphosphonium (TPP) cation based transporter system. In this system, TPP is linked, via a biolabile disulfide bridge, to an activated mercaptoethoxycarbonyl moiety, allowing its direct coupling to the N-terminal extremity of a free PNA through a ca...
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PMID: 18707540
PDF is available here.
PDF is available here.
Abstract
We isolated a partial zebrafish diaphanous-related formin 2 (zdia2) clone with its N-terminal regulatory domains. The GTPase binding domain of zDia2 is highly conserved compared to its mammalian homologues. Using a yeast two-hybrid assay, we showed that zDia2 interacts with constitutively-active Rho...
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PMID: 18941507
PDF is available here.
PDF is available here.
Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer (177) 2008
Abstract
Overexpression of the cytokine transforming growth factor-beta 2 (TGF-beta2) is a hallmark of various malignant tumors including pancreatic carcinoma, malignant glioma, metastasizing melanoma, and metastatic colorectal carcinoma. This is due to the pivotal role of TGF-beta2 as it regulates key mecha...
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PMID: 18084956
PDF is available here.
PDF is available here.
Abstract
We identified cold-inducible RNA binding protein (CIRP) as novel XTcf-3 specific target gene. Furthermore, we show that knockdown of XTcf-3 by injection of an antisense morpholino oligonucleotide results in a general broadening of the anterior neural tissue. Depletion of XCIRP by antisense morpholin...
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PMID: 18687117
PDF is available here.
PDF is available here.
BMC Bioinformatics (9)1 2008
Abstract
We have created a database to facilitate web-based distribution of data from such genetic studies. DESCRIPTION: The MOrpholino DataBase is a MySQL relational database with an online, PHP interface. Multiple quality control levels allow differential access to data in raw and finished formats. MODBv1...
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PMID: 18179718
PDF is available here.
PDF is available here.
Abstract
Rat MI model was generated by ligating the left anterior descending coronary artery. Four weeks later, left ventricular ejection fraction (LVEF), left ventricular systolic pressure (LVSP), the maximal rates of increase and decrease in intraventricular pressure (+/-dp/dt(max)) were significantly depr...
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PMID: 18096433
PDF is available here.
PDF is available here.
Abstract
Disseminated neuroectoderma-derived tumors, mainly neuroblastoma in childhood and melanoma in the adulthood, are refractory to most current therapeutic regimens and hence the prognosis remains very poor. Preclinical research studies have indicated several agents that show promising therapeutic poten...
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PMID: 18564613
PDF is available here.
PDF is available here.
Learning & Memory (15)9 2008
Abstract
Infusions of CREB antisense into the amygdala prior to training impair memory for aversive tasks, suggesting that the antisense may interfere with CRE-mediated gene transcription and protein synthesis important for the formation of new memories within the amygdala. However, the amygdala also appears...
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PMID: 18772255
PDF is available here.
PDF is available here.
Abstract
Mammalian gene expression can be regulated through various post-transcriptional events, including altered mRNA stability, translational control, and RNA-processing events such as 3'-end formation or polyadenylation (pA). It has become clear in recent years that pA is governed by several core sequenc...
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PMID: 18369983
PDF is available here.
PDF is available here.
Molecular Vision (14) 2008
Abstract
AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes....
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PMID: 18253092
PDF is available here.
PDF is available here.
Abstract
We have investigated the role of the ATP-dependent chromatin remodelling complex Isw2 in positioning nucleosomes. We find that Isw2 functions adjacent to promoter regions where it repositions nucleosomes at the interface between genic and intergenic sequences. Nucleosome repositioning by Isw2 is dir...
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PMID: 18075583
PDF is available here.
PDF is available here.
Plant Physiology (145)4 2007
Abstract
We analyzed the role of the sucrose (Suc) synthase MtSucS1 during nodulation of the model legume Medicago truncatula, integrating data for the developmental, transcriptional, and metabolic processes affected downstream of an impaired Suc cleavage in root nodules. To reduce carbohydrate supply to nod...
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PMID: 17951459
PDF is available here.
PDF is available here.
Plant Physiology (145)4 2007
Abstract
We investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa). In SDs OsMADS51 null mutants flowered 2 weeks later than normal, whereas in long days loss of OsMADS51 had little effect on flowering. Transcript levels of three flowering reg...
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PMID: 17951465
PDF is available here.
PDF is available here.
Transplantation Proceedings (39)10 2007
Abstract
Blockade of CD80-CD28 costimulatory pathway induces unresponsiveness of T cells to alloantigens and protects allografts against immune rejection in numerous animal models. The aim of this study was to investigate whether blocking expression of B7.1 (CD80) on donor splenocytes by an antisense techniq...
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PMID: 18089391
PDF is available here.
PDF is available here.
Plant Journal (52)6 2007
Abstract
We recently reported the downregulation of both the Cel1 mRNA and protein upon fungal infection, suggesting the involvement of EGases in plant-pathogen interactions. This hypothesis was confirmed by assessing the resistance to Botrytis cinerea infection of transgenic plants expressing both genes in...
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PMID: 17916112
PDF is available here.
PDF is available here.
Abstract
GDNF supports expression of both rhodopsin and THase in vitro, two critical molecules involved in the production of rod photoreceptors and dopaminergic amacrine cells, respectively; however, the presence of GDNF does not affect cone production and survival....
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PMID: 17962487
PDF is available here.
PDF is available here.
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (15)10 2007
Abstract
Plant Physiology (144)4 2007
Abstract
We have transformed Flaveria bidentis (a C(4) dicot) with antisense or RNA interference constructs targeted at the mRNA of this PEPC-PK. We generated several independent transgenic lines where PEPC is not phosphorylated in the light, demonstrating that this PEPC-PK is essential for the phosphorylati...
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PMID: 17586687
PDF is available here.
PDF is available here.
Plant Physiology (144)4 2007
Abstract
Pectinesterase (PE; E.C. 3.1.1.11) is an enzyme responsible for the demethylation of galacturonyl residues in high-molecular-weight pectin and is believed to play an important role in cell wall metabolism. In this study, Pmeu1, a ubiquitously expressed PE gene, has been characterized by antisense su...
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PMID: 17556513
PDF is available here.
PDF is available here.
Abstract
Results demonstrated that transfection of antisense p38 alpha gene diminishes myocardial cell injury mediated by hypoxia and burn serum, suggesting a new target for the prevention and treatment of myocardial damage after burn....
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PMID: 17467911
PDF is available here.
PDF is available here.
Plant Journal (51)3 2007
Abstract
Cotton (Gossypium hirsutum L.) fibers, one of the most important natural raw materials for textile industry, are highly elongated trichomes from epidermal cells of cotton ovules. DET2, an Arabidopsis steroid 5d-reductase, is considered to catalyze a major rate-limiting in brassinosteroid (BR) biosyn...
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PMID: 17565582
PDF is available here.
PDF is available here.
Plant Journal (51)3 2007
Abstract
We have isolated GPT1 from Vicia narbonensis and studied its role in seed development using a transgenic approach based on the seed-specific legumin promoter LeB4. GPT1 is highly expressed in vegetative sink tissues, flowers and young seeds. In the embryo, localized upregulation of GPT1 at the onset...
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PMID: 17587237
PDF is available here.
PDF is available here.
Molecular Endocrinology (21)7 2007
Abstract
G protein-coupled receptor 39 (GPR39) is a constitutively active, orphan member of the ghrelin receptor family that is activated by zinc ions. GPR39 is here described to be expressed in a full-length, biologically active seven-transmembrane form, GPR39-1a, as well as in a truncated splice variant fi...
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PMID: 17488974
PDF is available here.
PDF is available here.
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (15)7 2007
Abstract
Compared with the empty plasmid control group and the disease control group, the deposition of type I collagen decreased in the three antisense treatment groups: antisense TBRI group (4.37+/-1.30) x 10(5), P less than 0.05; antisense TIMP-1 group (3.40+/-0.91) x 10(5), probability value less than 0....
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PMID: 17669236
PDF is available here.
PDF is available here.
Plant Journal (51)1 2007
Abstract
We have defined in vitro conditions affecting the growth and recovery of Medicago truncatula roots after a salt stress. A dedicated macroarray containing 384 genes, based on a large-scale subtractive hybridization approach, was constructed and used to analyze gene expression during salt stress and r...
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PMID: 17488237
PDF is available here.
PDF is available here.
Abstract
We induced granulocyte differentiation by treating HL-60 cells with dimethyl sulphoxide, and erythroid or megakaryocytic differentiation by treating K562 cells with butyrate or phorbol myristate acetate, respectively. Nitro blue tetrazolium reduction, expression of haem, or expression of CD41 was us...
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PMID: 17408467
PDF is available here.
PDF is available here.
Abstract
We report that PNA(G1138) or peptide-PNA(G1138) targeted to domain II of 23S rRNA can inhibit both translation in vitro (in a cell-free translation system) and bacterial growth in vivo. The inhibitory concentration (IC50) and the minimum inhibiting concentration (MIC) are 0.15 and 10 microM, respect...
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PMID: 17266023
PDF is available here.
PDF is available here.
Molecular Cell (25)6 2007
Abstract
We describe here the use of protein design to show experimentally that a minimal class I aminoacyl-tRNA synthetase active site might have functioned in the distant past. We deleted the anticodon binding domain from tryptophanyl-tRNA synthetase and fused the discontinuous segments comprising its acti...
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PMID: 17386262
PDF is available here.
PDF is available here.
Abstract
We developed antisense peptide nucleic acids (PNAs) conjugated to the (KFF)(3)K peptide and investigated whether they could mediate gene-specific antisense effects in K. pneumoniae. No outer membrane permeabilization was observed with antisense PNAs when used alone. Antisense peptide-PNAs targeted a...
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PMID: 17158940
PDF is available here.
PDF is available here.
Abstract
We used this method to identify a beta-catenin-regulated antisense RNA expressed in HCT116 colorectal carcinoma cells, a cellular model of activated beta-catenin signaling. Genomic signature tags designating putative beta-catenin-binding sites mapped to the 3'-untranslated region (3'-UTR) of the E2F...
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PMID: 17121828
PDF is available here.
PDF is available here.
Abstract
The present report is a large series examining SSTR mRNA levels in somatotropinomas and NFPA. These initial findings suggest that detailed knowledge of the SSTR mRNA expression profile in somatotropinomas can help to predict the hormonal response to therapy with LAR. Also, it appears that SSTR3 in N...
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PMID: 17218727
PDF is available here.
PDF is available here.
Journal of Experimental Botany (58)15-16 2007
Abstract
In plants, betaine is synthesized upon abiotic stress via choline oxidation, in which choline monooxygenase (CMO) is a key enzyme. Although it had been thought that betaine synthesis is well regulated to protect abiotic stress, it is shown here that an exogenous supply of precursors such as choline,...
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PMID: 18182425
PDF is available here.
PDF is available here.
Abstract
The RNase protection assay is a standard approach to determine mRNA levels of a gene of interest in different tissues, developmental stages, or times of the day. Splicing or promoter variants can be studied with specific probes. It is widely used in chronobiology to study the temporal profile of exp...
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PMID: 17417021
PDF is available here.
PDF is available here.
Biochemistry (Washington) (45)50 2006
Abstract
We used the cell-penetrating alpha-helical amphipathic peptide KLAL KLAL KAL KAAL KLA-NH2 [model amphipathic peptide (MAP)] which was varied with respect to charge and structure-forming properties. Furthermore, the linkage and the localization of the attached peptide (C- vs N-terminal) were modified...
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PMID: 17154532
PDF is available here.
PDF is available here.
Current Drug Targets (7)12 2006
Abstract
We will discuss novel efforts to target the RNA intermediates of the HIV life cycle....
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PMID: 17168835
PDF is available here.
PDF is available here.
Molecular Microbiology (62)6 2006
Abstract
We provide evidence that FtsW of Mycobacterium tuberculosis (FtsWMTB) interacts with PBP3 through two extracytoplasmic loops. Pro306 in the larger loop and Pro386 in the smaller loop of FtsW are crucial for these interactions. Fluorescence microscopy shows that conditional silencing of ftsW in Mycob...
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PMID: 17427288
PDF is available here.
PDF is available here.