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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
RNA Precursors (0):
Recent article
RNA Precursors
Xenobiotica (41)5 2011
Abstract
We provide step-by-step methods on how to utilize synthetic precursor and antisense inhibitor molecules for understanding miRNA function....
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PMID: 18369991
PDF is available here.
PDF is available here.
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Abstract
Abstract
We systematically queried the Saccharomyces cerevisiae proteome for Prp19 WD40 domain interaction partners by two-hybrid analysis. We report that in addition to S. cerevisiae Cwc2, the splicing factor Prp17 binds directly to the Prp19 WD40 domain in a 1:1 ratio. Prp17 binds simultaneously with Cwc2...
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PMID: 21386897
PDF is available here.
PDF is available here.
EMBO Journal (29)24 2010
Abstract
Star-PAP is a poly (A) polymerase (PAP) that is putatively required for 3'-end cleavage and polyadenylation of a select set of pre-messenger RNAs (mRNAs), including heme oxygenase (HO-1) mRNA. To investigate the underlying mechanism, the cleavage and polyadenylation of pre-mRNA was reconstituted wit...
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PMID: 21102410
PDF is available here.
PDF is available here.
Abstract
RNA editing by adenosine deamination is a form of epigenetic control of gene expression wherein the ADAR enzymes convert adenosine to inosine in RNA often changing the meaning of codons. The pre-mRNA for the 2c subtype of serotonin receptor (5-HT2cR) is shown here to support small molecule binding n...
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PMID: 20820662
PDF is available here.
PDF is available here.
Abstract
We constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specifi...
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PMID: 20180025
PDF is available here.
PDF is available here.
Molecular Cell (39)3 2010
Abstract
We developed an in vitro assay sensitive to proofreading of 5' splice site cleavage. We inactivated spliceosomes by disrupting a metal-ligand interaction at the catalytic center and discovered that, when the DEAH box ATPase Prp16 was disabled, these spliceosomes catalyzed 5' splice site cleavage but...
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PMID: 20705241
PDF is available here.
PDF is available here.
Abstract
We propose a post-transcriptional regulon model that explains hnRNP E1's widespread functional diversity....
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PMID: 20584894
PDF is available here.
PDF is available here.
Abstract
We systematically searched for enzymes that modify arginines by the addition of methyl groups. Protein arginine methyltransferases (PRMTs) are such enzymes that transfer methyl groups from S-adenosyl methionine to arginine residues within polypeptide chains resulting in mono- or dimethylated arginin...
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PMID: 20562214
PDF is available here.
PDF is available here.
Abstract
We have characterized SL RNAs from Prionchulus punctatus. Surprisingly, this revealed the presence of a set of SLs that show clear sequence similarity to the SL2 family of spliced leaders, which have previously only been found within the rhabditine group (which includes C. elegans). Expression of on...
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PMID: 20566669
PDF is available here.
PDF is available here.
Abstract
We discuss the identification and characterization of the yeast NTC and review recent work in yeast that supports the essential role for this complex in the regulation and fidelity of splicing....
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PMID: 20659013
PDF is available here.
PDF is available here.
Abstract
We show by RNAPII chromatin immunoprecipitation (ChIP) that internal operon gene 5' ends do indeed lack Ser-5(P) peaks. In contrast, Ser-2(P) peaks occur at each mRNA 3' end, where the 3'-end formation machinery binds. These results provide additional support for the idea that the serine phosphoryla...
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PMID: 20498277
PDF is available here.
PDF is available here.
Development (137)14 2010
Abstract
We demonstrate that Drosophila SmB and SmD3 are specific components of the oskar messenger ribonucleoprotein (mRNP), proper localization of which is required for establishing germline fate and embryonic patterning. Importantly, oskar mRNA is delocalized in females harboring a hypomorphic mutation in...
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PMID: 20570937
PDF is available here.
PDF is available here.
Abstract
We identified a Dicer-independent miRNA biogenesis pathway that uses Argonaute2 (Ago2) slicer catalytic activity. In contrast to other miRNAs, miR-451 levels were refractory to dicer loss of function but were reduced in MZago2 (maternal-zygotic) mutants. We found that pre-miR-451 processing requires...
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PMID: 20448148
PDF is available here.
PDF is available here.
Abstract
We conducted a genetic screen to identify factors required for RNA interference (RNAi) in Caenorhabditis elegans nuclei. Here we show that the gene nuclear RNAi defective-2 (nrde-2) encodes an evolutionarily conserved protein that is required for siRNA-mediated silencing in nuclei. NRDE-2 associates...
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PMID: 20543824
PDF is available here.
PDF is available here.
Abstract
We show that the initiator-tRNA may also act, in a manner that is independent of its role in protein translation, as a pre-mRNA splicing regulator. Specifically, we show that alternative splicing events that are induced by mutations in the translation initiation AUG codon can be suppressed by expres...
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PMID: 20534564
PDF is available here.
PDF is available here.
Journal of General Virology (91)Pt 6 2010
Abstract
These results further suggest that HCMV infection selectively induces sorting of nucleolar and nucleoplasmic components....
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PMID: 20164265
PDF is available here.
PDF is available here.
Abstract
We have found that the ribosome can translate mutated lariat intermediates. By glycerol gradient analysis, we have found that the spliceosome can dissociate mutated lariat intermediates in vivo in a manner that requires the DEAH box ATPase Prp43p. Through an in vitro assay, we demonstrate that Prp43...
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PMID: 20463285
PDF is available here.
PDF is available here.
Abstract
We report a novel finding of an antisense oligonucleotide (ASO) microwalk in which we examined the position-specific role of intronic residues downstream from the 5' splice site (5' ss) of SMN2 exon 7, skipping of which is associated with spinal muscular atrophy (SMA), a leading genetic cause of inf...
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PMID: 20413618
PDF is available here.
PDF is available here.
Nucleic Acids Research (38)11 2010
Abstract
We report the first example where Hu proteins promote inclusion of an alternative exon, exon 6 of the HuD pre-mRNA. Sequence alignment analysis indicates the presence of several conserved AU-rich sequences both upstream and downstream to this alternatively spliced exon. We generated a human HuD exon...
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PMID: 20159993
PDF is available here.
PDF is available here.
Abstract
We evaluate the effect of five different drugs, previously found to modify splicing in several genetic disorders, on the splicing of mutated NF1 alleles. For this purpose, cell lines derived from patients bearing 19 different NF1-splicing defects were used. Our results showed that kinetin partially...
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PMID: 19935827
PDF is available here.
PDF is available here.
Metabolic Engineering (12)3 2010
Abstract
We generated a genomic library from sheared Clostridium acetobutylicum ATCC 824 DNA, whereby inserts can be expressed in both directions from the thiolase promoter, P(thl). Serial transfer of library-bearing C. acetobutylicum cultures exposed to increasing butyrate concentrations enriched for insert...
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PMID: 20060060
PDF is available here.
PDF is available here.
Developmental Dynamics (239)5 2010
Abstract
We identify prp-17 as another gene functioning downstream of GLP-1 signaling that promotes meiotic entry, largely by acting on the GLD-1 pathway, and that also functions in female germline sex determination. PRP-17 is orthologous to the yeast and human pre-mRNA splicing factor PRP17/CDC40 and can re...
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PMID: 20419786
PDF is available here.
PDF is available here.
Abstract
We recently found that the C/D box snoRNA HBII-52 changes the alternative splicing of the serotonin receptor 2C pre-mRNA, which is different from the traditional C/D box snoRNA function in non-mRNA methylation. Using bioinformatic predictions and experimental verification, we identified five pre-mRN...
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PMID: 20053671
PDF is available here.
PDF is available here.
Abstract
We demonstrated a direct role for histone modifications in alternative splicing. We found distinctive histone modification signatures that correlate with the splicing outcome in a set of human genes, and modulation of histone modifications causes splice site switching. Histone marks affect splicing...
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PMID: 20133523
PDF is available here.
PDF is available here.
Abstract
Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathoge...
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PMID: 19948855
PDF is available here.
PDF is available here.
Biochemical Society transactions (38)Pt 1 2010
Abstract
Nuclear RNA processing is a critical stage in eukaryotic gene expression, and is controlled in part by the expression and concentration of nuclear RNA-binding proteins. Different nuclear RNA-binding proteins are differentially expressed in different cells, helping the spliceosome to decode pre-mRNAs...
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PMID: 20074067
PDF is available here.
PDF is available here.
Abstract
We generated a simple amiRNA vector (pAmiR169d) based on the structure of Arabidopsis miR169d precursor (pre-miR169d). Two unique restriction sites were created inside the stem region of pre-miR169d, which allows for the artificial miRNA sequences to be cloned as either ~80 bp synthetic oligonucleot...
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PMID: 19693698
PDF is available here.
PDF is available here.
Methods in Enzymology (470) 2010
Abstract
We will describe methods that have been developed in our lab and others to examine pre-mRNA splicing from a genome-wide perspective in yeast. Our goal is to provide all of the necessary information--from microarray design to experimental setup to data analysis--to facilitate the widespread use of th...
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PMID: 20946806
PDF is available here.
PDF is available here.
Abstract
We discuss our latest findings indicating that KSRP is an integral component of both Drosha and Dicer complexes. KSRP binds to the terminal loop sequence of a subset of miRNA precursors promoting their maturation. Our data indicate that the terminal loop is a pivotal structure where activators of mi...
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PMID: 21627028
PDF is available here.
PDF is available here.
Abstract
We discuss how Lin28/LIN-28 can sequester pre-let-7 miRNA precursor to prevent Dicer-mediated processing. We describe how interaction of pre-let-7 with Lin28/ LIN-28 leads to pre-let-7 uridylation and subsequent degradation. Finally, we analyze how let-7 and Lin28/LIN-28 together act as a highly con...
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PMID: 21627031
PDF is available here.
PDF is available here.
Tsitologiia (52)1 2010
Abstract
We obtained the data on the distributions of poly(A)+RNA, hnRNPS A/B, and NXF1/TAP factor of mRNA export. These factors were found to associate with IGCs of mouse preovulatory oocytes. In the present study, we have demonstrated for the first time the dynamics of large IGCs after specific phosphorila...
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PMID: 20302015
PDF is available here.
PDF is available here.
Abstract
Proximal spinal muscular atrophy (SMA) is a neuromuscular disease caused by low levels of the survival motor neuron (SMN) protein. The reduced SMN levels are due to loss of the survival motor neuron-1 (SMN1) gene. Humans carry a nearly identical SMN2 gene that generates a truncated protein, due to a...
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PMID: 20515750
PDF is available here.
PDF is available here.
Molecular Vision (16) 2010
Abstract
To investigate the consequence of a major intrinsic protein MIP splice-site mutation (c.607-1G>A) in a four-generation Chinese pedigree afflicted with autosomal dominant congenital cataracts (ADCC).
Both a mutated minigene with c.607-1G>A, and a wild-type minigene were constructed using the pTARGET...
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PMID: 21139677
PDF is available here.
PDF is available here.
Trends in Cell Biology (20)1 2010
Abstract
The expression of protein-coding genes is enhanced by the exquisite coupling of transcription by RNA polymerase II with pre-messenger RNA processing reactions, such as 5'-end capping, splicing and 3'-end formation. Integration between cotranscriptional processing events extends beyond the nucleus, a...
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PMID: 20004579
PDF is available here.
PDF is available here.
Abstract
The gene transformer (tra) is the key regulatory memory device for sex determination in tephritid insects. The present manuscript addressed the question about the functional conservation of the tephritid Anastrepha Transformer protein to direct somatic sexual development in Drosophila (Drosophilidae...
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PMID: 20209435
PDF is available here.
PDF is available here.
Abstract
Recombinant human ribosomal protein S16 (rpS16) is shown to bind specifically to a fragment of its own pre-mRNA that includes exons 1 and 2, intron 1, and part of intron 2, and to inhibit the splicing of the fragment in vitro. The weaker binding of other recombinant human ribosomal proteins, S10 and...
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PMID: 20198863
PDF is available here.
PDF is available here.
Abstract
Transacting siRNA (tasiRNA) biogenesis in Arabidopsis is initiated by microRNA (miRNA) -guided cleavage of primary transcripts. In the case of TAS3 tasiRNA formation, ARGONAUTE7 (AGO7)-miR390 complexes interact with primary transcripts at two sites, resulting in recruitment of RNA-DEPENDENT RNA POLY...
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PMID: 20018656
PDF is available here.
PDF is available here.
Abstract
We use HeLa nuclear extracts to study two of these activities, poly(A) signal-dependent transcriptional pausing, which was originally proposed as a surveillance checkpoint, and poly(A) signal-dependent degradation (PDD) of unprocessed transcripts from weak poly(A) signals. We confirm directly, by me...
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PMID: 19926725
PDF is available here.
PDF is available here.
Abstract
We show that after infection of human cells with herpes simplex virus type 1 (HSV-1) the rate of ribosome biogenesis is modulated independently of RNA Pol I activity by a dramatic change in the rRNA maturation pathway. This process permits control of the ribosome biogenesis rate, giving the possibil...
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PMID: 19934231
PDF is available here.
PDF is available here.
BMC Genomics (11) 2010
Abstract
Many plant species have been investigated in the last years for the identification and characterization of the corresponding miRNAs, nevertheless extensive studies are not yet available on barley (at the time of this writing). To extend and to update information on miRNAs and their targets in barley...
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PMID: 20969764
PDF is available here.
PDF is available here.
Abstract
We investigated the satellite cell nuclei of biceps and quadriceps muscles from adult and old rats; morphometry and immunocytochemistry at light and electron microscopy have been combined to assess the organization of the nuclear RNP structural constituents involved in different steps of mRNA format...
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PMID: 20490357
PDF is available here.
PDF is available here.
Abstract
We hypothesized that common variants in the miRNAs are associated with risk of coal workers' pneumoconiosis (CWP). In a case-control study of 496 CWP patients and 513 control subjects frequency matched by exposure years and work types, we genotyped four single-nucleotide polymorphisms (SNPs) (rs2910...
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PMID: 19881472
PDF is available here.
PDF is available here.
Abstract
We expressed a series of mutated alleles of NOC4 in yeast cells and analysed whether the corresponding protein variants support vegetative growth, interact with Nop14p, and are incorporated into the SSU-processome. The data reveal that the essential C-terminus of Noc4p which contains 237 aminoacids...
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PMID: 20019888
PDF is available here.
PDF is available here.
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Abstract
We report the cloning and identification of approximately 40 new putative miRs from local basmati rice variety in accordance to the annotation suggested by Meyers et al. (Plant Cell 20:3186-3190, 2008). About 23 sequences were derived from rice exposed to salt stress while 18 were derived from rice...
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PMID: 20131478
PDF is available here.
PDF is available here.
Nature Methods (6)11 2009
Abstract
We engineered artificial splicing factors by combining sequence-specific RNA-binding domains of human Pumilio1 with functional domains that regulate splicing. We applied these factors to modulate different types of alternative splicing in selected targets, to examine the activity of effector domains...
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PMID: 19801992
PDF is available here.
PDF is available here.
Neuromolecular Medicine (11)3 2009
Abstract
We review evidence from studies of adult mouse forebrain supporting a model wherein synaptic stimulation (above a threshold value) increases calcium within dendritic spines, activates calpain, and activates and releases dicer from the postsynaptic density. Dicer processes local pre-miRs into mature...
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PMID: 19458942
PDF is available here.
PDF is available here.
Biochemical Journal (422)3 2009
Abstract
We report that both sptrz1(+) and sptrz2(+) are essential for growth. Moreover, sptrz1(+) is required for cell viability in the absence of Sla1p, which is thought to be required for endonuclease-mediated maturation of pre-tRNA 3' ends in yeast. Both scTRZ1 and ELAC2 can complement a temperature-sens...
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PMID: 19555350
PDF is available here.
PDF is available here.
Comprehensive analysis of the impact of SNPs and CNVs on human microRNAs and their regulatory genes.
Abstract
We identified 380 (53.9%) precursor miRNAs (pre-miRNAs) embedded in gene loci that are enriched in biological processes such as "neuronal activities", "cell cycle" and "protein phosphorylation" (Bonferroni p < 0.05). Gene lengths of the pre-miRNA host genes are significantly larger than other genes...
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PMID: 19458495
PDF is available here.
PDF is available here.
Abstract
We provide evidence that recombinant Nob1 forms a tetramer that binds directly to pre-rRNA analogs containing cleavage site D. Analysis of Nob1's affinity to a series of RNA truncations, as well as Nob1-dependent protections of pre-rRNA in vitro and in vivo demonstrate that Nob1's binding site cente...
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PMID: 19706509
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)32 2009
Abstract
We showed that galectin-1 and galectin-3 are redundant pre-mRNA splicing factors associated with the spliceosome throughout the splicing pathway. Here we present evidence for the association of galectin-3 with snRNPs outside of the spliceosome (i.e., in the absence of pre-mRNA splicing substrate). I...
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PMID: 19603745
PDF is available here.
PDF is available here.
Abstract
Through alternative splicing, multiple different transcripts can be generated from a single gene. Alternative splicing represents an important molecular mechanism of gene regulation in physiological processes such as developmental programming as well as in disease. In cancer, splicing is significant...
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PMID: 19574427
PDF is available here.
PDF is available here.