Abstract
We discovered 16 drought stress-associated miRNA (DsAmR) families in P. patens through computational analysis. Due to the possible discrepancy of expression periods and tissue distributions between potential DsAmRs and their targeting genes, and the existence of false positive results in computation...
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PMID: 21641561
PDF is available here.
Abstract
We report here that the expression of endogenous microRNAs (miRNAs) can be efficiently silenced in Arabidopsis thaliana (Arabidopsis) using artificial miRNA (amiRNA) technology. We demonstrate that an amiRNA designed to target a mature miRNA directs silencing against all miRNA family members, wherea...
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PMID: 20943811
PDF is available here.
Abstract
Transient populations of cis- and trans-acting small RNAs have recently emerged as key regulators of extensive epigenetic changes taking place during periconception, which encompasses gametogenesis, fertilization, and early zygotic development. These small RNAs are not only important to maintain gen...
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PMID: 21030645
PDF is available here.
Abstract
Paramutation refers to the process by which homologous DNA sequences communicate in trans to establish meiotically heritable expression states. Although mechanisms are unknown, current data are consistent with the hypothesis that the establishment and heritable transmission of specific chromatin sta...
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PMID: 21030647
PDF is available here.
Abstract
We show that in virus-infected plants the enhanced expression of AGO1 mRNA is not accompanied by increased AGO1 protein accumulation. We also show that the induction of AGO1 mRNA level is a part of the host defence reaction, whereas the induction of miR168, which overlaps spatially with virus-occupi...
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PMID: 20823831
PDF is available here.
Abstract
We recently performed a genome-wide survey for endogenous targets silenced by MOM1 and identified loci that are concentrated around centromeres and rich in sequences homologous to the 24-nt small interfering RNAs (siRNAs) that accumulate in wild type plants. Further and independent analyses indicate...
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PMID: 21464619
PDF is available here.
Abstract
MicroRNAs (miRNAs) are 21-23 nucleotide (nt) non-coding RNAs that play a key role in regulating the expression of protein-coding genes at post-transcriptional levels in plants and animals. MiRNA genes, which serve as genetic buffers and regulators, are primarily located in the intergenic regions of...
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PMID: 20691276
PDF is available here.
Abstract
Several key enzymes in lignin biosynthesis of Populus have been down-regulated by transgenic approaches to investigate their role in wood lignification and to explore their potential for lignin modification. Cinnamate 4-hydroxylase is an enzyme in the early phenylpropanoid pathway that has not yet b...
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PMID: 20831275
PDF is available here.
Abstract
We isolated the full-length cDNA of AhIRT1 (iron-regulated transporter 1) from peanut and characterized how iron deficiency and intercropping affected its iron-transporting ability. Functional complementation with AhIRT1 restored normal growth of the yeast mutant fet3fet4 (defective in both high- an...
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PMID: 20430476
PDF is available here.
Abstract
We reinvestigated the relationship between auxin-induced elongation and gene expression of glucanases upon treatment of coleoptile segments with sugars. Gene expression of exo-beta-1,3:1,4-glucanase ExoII was not affected by treatment with IAA and/or sucrose. In contrast, levels of endo-beta-1,3:1,4...
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PMID: 20412461
PDF is available here.
Abstract
Plants have evolved a variety of gene silencing pathways mediated by small RNAs. Mostly 21 or 24 nt in size, these small RNAs repress the expression of sequence homologous genes at the transcriptional, post-transcriptional and translational levels. These pathways, also referred as RNA silencing path...
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PMID: 20331529
PDF is available here.
Abstract
We show that the formation of polymeric CrSUMO96 and CrSUMO97 chains can be catalyzed either by the human SAE1/SAE2 and Ubc9 SUMOylation system in vitro or by an Escherichia coli chimeric SUMOylation system in vivo. An exposed C-terminal di-glycine motif of CrSUMO96 or CrSUMO97 is essential for func...
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PMID: 20544217
PDF is available here.
Abstract
We isolated four distinct avocado cell proliferation-related genes and investigated their expression characteristics, comparing normal fruit (NF) and SF developmental patterns. Three cDNAs termed PaCYCA1, PaCYCB1 and PaPCNA, encoding two mitotic cyclins and a proliferating cell nuclear antigen (PCNA...
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PMID: 20544218
PDF is available here.
Abstract
Infestation of wheat by Hessian fly larvae causes a variety of physical and biochemical modifications of the host plant. Changes occur in cuticle permeability, lipid composition and gene transcript abundance, and these responses differ substantially between resistant and susceptible...
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PMID: 20409001
PDF is available here.
Yang-Seok Lee,
Dong-Hoon Jeong,
Dong-Yeon Lee,
Jakyung Yi,
Choong-Hwan Ryu,
Song L Kim,
Hee J Jeong,
Sang C Choi,
Ping Jin,
Jungil Yang,
Lae-Hyeon Cho,
Heebak Choi and
Gynheung An
Abstract
We investigated the functioning of OsCOL4, a member of the CONSTANS-like (COL) family in rice. OsCOL4 null mutants flowered early under short or long days. In contrast, OsCOL4 activation-tagging mutants (OsCOL4-D) flowered late in either environment. Transcripts of Ehd1, Hd3a, and RFT1 were increase...
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PMID: 20409004
PDF is available here.
Abstract
We analyzed the effect of exogenously applied plant growth regulators, namely gibberellic acid (GA(3)) and daminozide, on leaf morphology and essential oil formation of two leaf stages during the period of leaf expansion. Essential oil content increased with increasing levels of gibberellins and dec...
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PMID: 20163890
PDF is available here.
Abstract
Plants that constitutively express otherwise inducible disease resistance traits often suffer a depressed seed yield in the absence of a challenge by pathogens. This has led to the view that inducible disease resistance is indispensable, ensuring that minimal resources are diverted from growth, repr...
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PMID: 20573051
PDF is available here.
Hyeong Cheol Park,
Hun Kim,
Sung Cheol Koo,
Hee Jin Park,
Mi Sun Cheong,
Hyewon Hong,
Dongwon Baek,
Woo Sik Chung,
Doh Hoon Kim,
Ray A Bressan,
Sang Yeol Lee,
Hans J Bohnert and
Dae-Jin Yun
Abstract
We report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugate...
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PMID: 20561251
PDF is available here.
Abstract
Three vacuolar cation/H+ antiporters, AtNHX1 (At5g27150), 2 (At3g05030) and 5 (At1g54370), have been characterized as functional Na+/H+ antiporters in Arabidopsis. However, the physiological functions of AtNHX3 (At5g55470) still remain unclear. In this study, the physiological functions of AtNHX3 we...
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PMID: 20573049
PDF is available here.
Abstract
We used a molecular approach to characterize ureide accumulation under drought stress in Phaseolus vulgaris. Accumulation of ureides, mainly allantoate, was found in roots, shoots and leaves, but only a limited transient increase was observed in nodules from drought-stressed plants. We show that ure...
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PMID: 20545885
PDF is available here.
Abstract
We measured the O₂ uptake rate, activity and transcript level of respiratory components, and concentration of adenylates using Arabidopsis thaliana shoots grown in media containing various N sources. The O₂ uptake rate was correlated with concentrations of ammonium and ATP in shoots, but not rel...
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PMID: 20545883
PDF is available here.
Abstract
We hypothesize that AtPAP12 and AtPAP26 facilitate Pi scavenging from soil-localized organophosphates during nutritional Pi deprivation.
© 2010 Blackwell Publishing Ltd....
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PMID: 20545876
PDF is available here.
Abstract
These results clearly indicate that the activity of SSII in sweetpotato storage roots, like those in other plants, affects the pasting properties of starch through alteration of the amylopectin structure....
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PMID: 20306051
PDF is available here.
Abstract
Maize seeds are an important source of food, animal feed, and industrial raw materials. To understand global gene expression and regulation during maize seed development, a normalized cDNA library, covering most of the developmental stages of maize seeds, was constructed. Sequencing analysis of 10,8...
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PMID: 20336461
PDF is available here.
Abstract
Plants, and specially species adapted in non-friendly environments, produce secondary metabolites that help them to cope with biotic or abiotic stresses. These metabolites could be of great pharmaceutical interest because several of those show cytotoxic, antibacterial or antioxidant activities. Leav...
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PMID: 20364257
PDF is available here.
Abstract
We have investigated possible mechanisms by which UV-B could reactivate Mu transposons by monitoring transcript abundance, epigenetic DNA marks, and chromatin factors associated with these elements. We demonstrate that both mudrA and B transcripts are expressed at higher levels after an 8 h-UV-B tre...
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PMID: 20421734
PDF is available here.
Abstract
We identified 121 potential switchgrass miRNAs, belonging to 44 families, using a well-defined comparative genome-based computational approach. We also identified miRNA clusters and antisense miRNAs in switchgrass expressed sequences tags. These identified miRNAs potentially target 839 protein-codin...
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PMID: 20461402
PDF is available here.
Abstract
The SOS screen, as originally described by Perkins et al. (1999) [7], was setup with the aim of identifying Arabidopsis functions that might potentially be involved in the DNA metabolism. Such functions, when expressed in bacteria, are prone to disturb replication and thus trigger the SOS response....
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PMID: 20227352
PDF is available here.
Abstract
High temperature impairs grain filling by inhibiting the deposition of storage materials such as starch and protein. To comprehend its impact on grain filling metabolism in rice (Oryza sativa), levels of metabolites and transcripts related to central pathways of metabolism were simultaneously determ...
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PMID: 20304786
PDF is available here.
Abstract
We report the characterization of a novel aquaporin gene TaNIP (Triticum asetivum L. nodulin 26-like intrinsic protein), which was involved in salt tolerance pathways in plants. TaNIP was identified and cloned through the gene chip expression analysis of a salt-tolerant wheat mutant RH8706-49 under...
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PMID: 20360019
PDF is available here.
Abstract
We examined the effects of AOX deficiency on plant growth, gene expression of respiratory components and metabolic profiles under low-N stress, using an aox1a knockout transgenic line (aox1a) of Arabidopsis thaliana. We exposed wild-type (WT) and aox1a plants to low-N stress for 7 d and analyzed the...
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PMID: 20304787
PDF is available here.
Abstract
We identified a homolog of the RH36 gene, AtRH36, which encodes a DEAD-box protein in Arabidopsis thaliana. The gene was expressed ubiquitously throughout the plant. The AtRH36 fused to green fluorescent protein was localized in the nucleus. Homozygosity for the Arabidopsis atrh36 mutants, atrh36-1...
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PMID: 20378763
PDF is available here.
Abstract
In photosynthetic organisms chlorophyll and heme biosynthesis is tightly regulated at various levels in response to environmental adaptation and plant development. The formation of 5-aminolevulinic acid (ALA) is the key regulatory step and provides adequate amounts of the common precursor molecule f...
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PMID: 20375109
PDF is available here.
Abstract
We established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) and their target...
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PMID: 20375108
PDF is available here.
Abstract
We previously characterized proteins in the MOS4-associated complex (MAC) that function in the regulation of plant immune responses downstream of the autoactivated R protein snc1. The MAC is a highly conserved spliceosome-associated complex homologous to the nineteen complex (NTC) in yeast and human...
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PMID: 20360018
PDF is available here.
Abstract
In plants, small RNA (microRNAs and other endogenous small RNAs)-guided target gene expression is vital for a wide variety of biological processes including adaptation to stress conditions. Identification of stress-regulated microRNAs or other classes of endogenous small RNAs advances our understand...
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PMID: 20387050
PDF is available here.
Abstract
We have developed a simple but effective array platform for profiling plant miRNAs from various plant species. The array is composed of 188 non-redundant miRNA probes that can detect both conserved and species-specific miRNAs from most plant species, including Arabidopsis, rice, and poplar. In this...
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PMID: 20387051
PDF is available here.
Abstract
We describe in detail the SSH procedure and explain the subtle changes that have been incorporated to make this technique adaptable for identifying stress-responsive genes in plants....
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PMID: 20387045
PDF is available here.
Akihiro Matsui,
Junko Ishida,
Taeko Morosawa,
Masanori Okamoto,
Jong-Myong Kim,
Yukio Kurihara,
Makiko Kawashima,
Maho Tanaka,
Taiko Kim To,
Kentaro Nakaminami,
Eli Kaminuma,
Takaho A Endo,
Yoshiki Mochizuki,
Shuji Kawaguchi,
Norio Kobayashi,
Kazuo Shinozaki,
Tetsuro Toyoda and
Motoaki Seki
Abstract
We applied the Arabidopsis Affymetrix tiling arrays to study the whole-genome transcriptome under drought, cold, and high-salinity stresses and identified a large number of drought, cold, and high-salinity stress-inducible genes and transcriptional units (TUs)....
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PMID: 20387044
PDF is available here.
Xu-xia Wang,
Bo Wang,
Li-jun Liu,
Xiao-ping Cui,
Jin-yu Yang,
Hui Wang,
Hui Jiang,
Bei-bei Luo,
Zhu Long,
Wen-xiang Dou,
Na Zhang and
Ding-xiang Peng
Abstract
These results showed that our protocol was applicable for RNA isolation from recalcitrant ramie tissues....
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PMID: 19680788
PDF is available here.
Abstract
We confirmed that PME was completely inhibited by TaPMEI. These results indicated that TaPMEI was involved in inhibition of pectin methylesterification and may play a role in the plant defense mechanism via cell wall fortification....
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PMID: 20558896
PDF is available here.
Susumu S Oda,
Fumi F Kaneko,
Kentaro K Yano,
Tomoaki T Fujioka,
Hiromi H Masuko,
Jong-In JI Park,
Shunsuke S Kikuchi,
Kazuki K Hamada,
Makoto M Endo,
Kuniaki K Nagano,
Yoshiaki Y Nagamura,
Makiko M Kawagishi-Kobayashi,
Keita K Suwabe,
Go G Suzuki and
Masao M Watanabe
Abstract
We analyzed morphological features and gene expression profiles, under cool temperature stress, in anther development of rice. Hitomebore was given cool temperature stress (19 degrees C) at flowering stage, and showed 87.3% seed fertility. Meanwhile, the seed fertility decreased to 41.7% in the case...
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PMID: 20558897
PDF is available here.
Abstract
We summarise the approaches currently available for localising RNA in vivo and address the specific limitations inherent with plant systems....
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PMID: 20153241
PDF is available here.
Abstract
We studied the LOX activity in damaged as well as in distal leaves in response to specialist (Agraulis vanillae vanillae) or generalist (Spodoptera frugiperda) insect attack. Enzymatic assays showed that induction of LOX activity occurred locally and systemically in response to both insects' attacks...
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PMID: 19995344
PDF is available here.
Abstract
We report on the first use of RNA-Seq to gain insight into the wide range of transcriptional responses that are associated with berry development in Vitis vinifera 'Corvina'. More than 59 million sequence reads, 36 to 44 bp in length, were generated from three developmental stages: post setting, vé...
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PMID: 20118272
PDF is available here.
Abstract
We describe the application of a PCR-based differential screening method to plant-fungal interactions. Suppression subtraction hybridization (SSH) provides a sensitive method to isolate fungal genes expressed in planta. Total RNA is isolated from infected plants for comparison with the pathogen in a...
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PMID: 20238264
PDF is available here.
Abstract
Laser microdissection (LM) has become an important tool for isolating individual cells or cell types from suitably prepared tissue samples. The technique can be used to isolate both fungal and host plant cells after pathogen infection for molecular studies. Sample preparation is a crucial step in LM...
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PMID: 20238267
PDF is available here.
Abstract
We describe a method to identify small RNA sequences from plant tissues. Small RNAs are purified by column fractionation and gel excision from total RNAs. These small RNAs are ligated at both termini to DNA/RNA chimeric adapters and reverse-transcribed to produce cDNAs. By the following PCR amplific...
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PMID: 20204873
PDF is available here.
Abstract
We provide protocols for the detection of miRNAs by stem-loop RT-PCR. This method enables fast and reliable miRNA expression profiling from as little as 20 pg of total RNA extracted from plant tissue and is suitable for high-throughput miRNA expression analysis. In addition, this method can be used...
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PMID: 20204872
PDF is available here.
Abstract
We address this problem by analysing the expression stability of eight potential tobacco reference genes. Primers targeting each gene (18S rRNA, EF-1alpha, Ntubc2, alpha- and beta-tubulin, PP2A, L25 and actin) were developed and optimized. The expression of each gene was then measured by real-time P...
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PMID: 20098998
PDF is available here.
Abstract
We report generation of ESTs, transcript profiles of selected genes and validation of candidate genes obtained from the subtracted cDNA libraries of pigeonpea plants subjected to PEG/water-deficit stress conditions. Cluster analysis of 124 selected ESTs yielded 75 high-quality ESTs. Homology searche...
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PMID: 20131066
PDF is available here.
Abstract
We demonstrate that HaFAD2-1 promoter is as strong as 35S promoter even though it is a tissue-specific promoter and its activity derived just from the embryo, thus confirming that it can be considered a strong highly specific seed promoter useful for biotechnology applications....
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PMID: 20084514
PDF is available here.
Abstract
We used Petunia hybrida flowers, which are rich in Phe-derived volatiles, to determine the biosynthetic routes involved in Phe formation in planta. Of the three identified petunia ADTs, expression of ADT1 was the highest in petunia petals and positively correlated with endogenous Phe levels througho...
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PMID: 20215586
PDF is available here.
Abstract
We have compared the transcriptomic profiles of microdissected live ovules at four developmental stages between a diploid sexual and diploid apomictic Boechera. We sequenced >2 million SuperSAGE tags and identified (1) heterochronic tags (n = 595) that demonstrated significantly different patterns o...
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PMID: 20305122
PDF is available here.
Abstract
We demonstrate that CRT1 interacts with various resistance proteins from different structural classes, and this interaction is disrupted when these resistance proteins are activated. The Arabidopsis mutant crt1-2 crh1-1, which lacks CRT1 and its closest homolog, displayed compromised resistance to a...
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PMID: 20332379
PDF is available here.
Abstract
We show that three MAMPs, the flagellar peptide Flg22, peptidoglycan, and chitin, trigger a strong tissue-specific response in Arabidopsis thaliana roots, either at the elongation zone for Flg22 and peptidoglycan or in the mature parts of the roots for chitin. Ethylene signaling, the 4-methoxy-indol...
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PMID: 20348432
PDF is available here.
Abstract
A double homozygous recessive mutant in the Arabidopsis thaliana homologs of nucleus- and phragmoplast-localized kinase 2 (ANP2) and 3 (ANP3) genes and a homozygous recessive mutant in the mitogen-activated protein kinase 4 (MPK4) gene of Arabidopsis exhibit deficiencies in the overall microtubule (...
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PMID: 20215588
PDF is available here.
Abstract
We show that GA-deficient (35S:PcGA2ox1) and GA-insensitive (35S:rgl1) transgenic Populus exhibited increased lateral root proliferation and elongation under in vitro and greenhouse conditions, and these effects were reversed by exogenous GA treatment. In addition, RNA interference suppression of tw...
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PMID: 20354195
PDF is available here.