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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
RNA (20):
Recent article
RNA
Molecular Cell (43)6 2011
Abstract
RNA molecules play diverse functional roles in natural biological systems. There has been growing interest in designing synthetic RNA counterparts for programming biological function. The design of synthetic RNA molecules that exhibit diverse activities, including sensing, regulatory...
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PMID: 21925380
PDF is available here.
PDF is available here.
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Eukaryotic Cell (10)9 2011
Abstract
We analyzed the structure of the macronuclear TtVPS13A gene, which was found to be composed of 17 exons spanning 12.5 kb and was predicted to encode a protein of 3,475 amino acids (aa). A strain expressing a TtVPS13A-green fluorescent protein (GFP) fusion protein was constructed, and the protein was...
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PMID: 21764909
PDF is available here.
PDF is available here.
Nature Reviews: Genetics (12)9 2011
Abstract
RNA structure is crucial for gene regulation and function. In the past, transcriptomes have largely been parsed by primary sequences and expression levels, but it is now becoming feasible to annotate and compare transcriptomes based on RNA structure. In addition to computational pred...
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PMID: 21850044
PDF is available here.
PDF is available here.
Abstract
Atherosclerosis is a chronic inflammatory process. Poly(ADP-ribose) polymerase-1 (PARP), a nuclear enzyme linked to DNA repair, has been shown to be involved in atherogenesis; however, the effects on dendritic cells, T cells and serum auto-antibody levels are not fully understood....
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PMID: 21813379
PDF is available here.
PDF is available here.
Integrative Biology (3)7 2011
Abstract
We show that, in addition to causing cell death of hormone-refractory prostate cancer cells, PJ also increases cell adhesion and decreases cell migration of the cells that do not die. We hypothesized that PJ does so by stimulating the expression and/or activation of molecules that alter the cytoskel...
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PMID: 21594291
PDF is available here.
PDF is available here.
Abstract
We describe structural and functional analyses of the Thermus thermophilus CRISPR Cse3 endonuclease. High-resolution X-ray structures of Cse3 bound to repeat RNAs model both the pre- and post-cleavage complexes associated with processing the pre-crRNA. These structures establish the molecular basis...
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PMID: 21572444
PDF is available here.
PDF is available here.
Abstract
We show that Cse3 type CRISPR-specific endoRNases bind a hairpin structure and residues downstream of the cleavage site within the repetitive segment of cognate CRISPR RNA. Cocrystal structures of Cse3-RNA complexes reveal an RNA-induced conformational change in the enzyme active site that aligns th...
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PMID: 21572442
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
The understanding of physiology and pathology requires accurate quantification of intracellular concentrations of important molecules such as unique RNA species. Accurate quantification of highly homologous messenger RNAs (mRNAs) (1-3), alternatively spliced mRNAs (4), and the short microRNAs (miRNA...
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PMID: 18982299
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Today the treatment of inherited diseases holds a major field in gene therapy, and gamma -retroviral vectors are often the preferred tool for stable introduction of the therapeutic gene(s) into the host cell genome. In many cases, the newly introduced gene has to be constitutively expressed, since e...
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PMID: 19110630
PDF is available here.
PDF is available here.
Abstract
In recent developments in chemistry and genetic engineering, the humble researcher dealing with the origin of life finds her(him)self in a grey area of tackling something that even does not yet have a clear definition agreed upon. A series of chemical steps is described to be conside...
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PMID: 21641557
PDF is available here.
PDF is available here.
Brain research (1381) 2011
Abstract
We postulated that gamma-amino butyric acid (GABA)- and acetylcholine (ACh)-related genes might be associated with the pathophysiology of TS. Total RNA isolated from whole blood of 26 un-medicated TS subjects and 23 healthy controls (HC) was processed on Affymetrix Human Exon 1.0 ST arrays. Data wer...
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PMID: 21241679
PDF is available here.
PDF is available here.
Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale (40)1 2011
Abstract
Our results reveal that AMCase and eotaxin-3 may be important mediators in the pathogenesis of nasal polyps. The increased AMCase and eotaxin-3 might lead to nasal polyp formation and growth. Future studies are needed to determine the potential of AMCase and eotaxin-3 as therapeutic targets in CRSwN...
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PMID: 21303604
PDF is available here.
PDF is available here.
Journal of Molecular Biology (405)3 2011
Abstract
We report a fluorescence-based assay for measuring the real-time kinetics of single-nucleotide incorporation during transcription elongation. The fluorescent adenine analogue 2-aminopurine was incorporated at various single positions in the template or the nontemplate strand of the promoter-free elo...
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PMID: 21035457
PDF is available here.
PDF is available here.
Abstract
We investigated expressional changes in the hippocampus and cortex at 72 h after infection when dexamethasone was given to infant rats with pneumococcal meningitis. Nursing Wistar rats were intracisternally infected with Streptococcus pneumoniae to induce experimental meningitis or were sham-infecte...
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PMID: 21412436
PDF is available here.
PDF is available here.
Abstract
We analysed for the first time the complete transcriptome of human trisomic endothelial progenitor cells to an unprecedented level of resolution and sensitivity by RNA-sequencing. Our analysis allowed us to detect differential expression of even low expressed genes crucial for the pathogenesis, to d...
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PMID: 21533138
PDF is available here.
PDF is available here.
Abstract
We analysed for the first time the complete transcriptome of human trisomic endothelial progenitor cells to an unprecedented level of resolution and sensitivity by RNA-sequencing. Our analysis allowed us to detect differential expression of even low expressed genes crucial for the pathogenesis, to d...
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PMID: 21533138
PDF is available here.
PDF is available here.
Journal of Immunotherapy (34)1 2011
Abstract
Electroporation of mature dendritic cells (DC) with RNA-encoding CD40L greatly enhances the production of interleukin (IL)-12, a proinflammatory cytokine necessary for the induction of T-cell immunity. Results presented herein reveal a correlation between the priming of CD28(+) antig...
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PMID: 21150712
PDF is available here.
PDF is available here.
Abstract
We here describe RNA extraction from one or a few frozen tissue sections and subsequent analysis of structural RNA integrity by microcapillary gel electrophoresis....
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PMID: 20949406
PDF is available here.
PDF is available here.
Abstract
We have used RNA interference to selectively suppress the expression of aggrecanase genes in human chondrocytes, in an attempt to determine which of these key enzymes have roles in arthritic cartilage destruction. This combination of gene targeting and tissue engineering we are using should be equal...
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PMID: 21042968
PDF is available here.
PDF is available here.
Abstract
We describe the capacity of mouse embryonic stem cells (mESCs) to differentiate into osteoblast-like cells in a three-dimensional (3D) self-assembling peptide scaffold, a synthetic nanofiber biomaterial with future applications in regenerative medicine. We have previously demonstrated that classical...
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PMID: 21042996
PDF is available here.
PDF is available here.
Abstract
We review the protocols for a yeast-based assay to detect effective ZFNs. Additionally, we detail the procedures for synthesis and injection of ZFN-encoding mRNA into zebrafish embryos, screening of injected embryos for induced mutations in the soma, and recovery of germline mutations....
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PMID: 21805278
PDF is available here.
PDF is available here.
Abstract
The rhesus macaque (RM) model has the potential to be an invaluable tool for studying B cell populations during pathogenic infections, however, to date, there has been no definitive delineation of naïve and memory B cell populations in the RM. This has precluded a rigorous analysis of the generatio...
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PMID: 20875419
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)53 2010
Abstract
These results suggest an alternative pathway in which Pif1 removes Okazaki fragments initiated by fold-back flaps in vivo....
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PMID: 20959454
PDF is available here.
PDF is available here.
EMBO Journal (29)24 2010
Abstract
U1 small nuclear ribonucleoprotein (snRNP) recognizes the 5'-splice site early during spliceosome assembly. It represents a prototype spliceosomal subunit containing a paradigmatic Sm core RNP. The crystal structure of human U1 snRNP obtained from natively purified material by in situ limited proteo...
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PMID: 21113136
PDF is available here.
PDF is available here.
Abstract
We searched for proteins that specifically bind to 8-oxoguanine-containing RNA from human HeLa cell extracts, and the candidate proteins were identified using mass spectrometry. Among the identified candidates, splicing isoform 1 of heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) and splicing is...
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PMID: 21073862
PDF is available here.
PDF is available here.
Abstract
We use Langevin dynamics to investigate the role played by the recently discovered force-induced entropic energy barrier on the two-state hopping phenomena that has been observed in single RNA, DNA and protein molecules placed under a stretching force. Simple considerations about the free energy of...
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PMID: 21050839
PDF is available here.
PDF is available here.
Abstract
We show that BRCA1 expression is controlled by a dynamic equilibrium between transcriptional coactivators and co-repressors that govern histone acetylation and DNA accessibility at the BRCA1 promoter. Eviction of the transcriptional co-repressor and metabolic sensor, C terminal-binding protein (CtBP...
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PMID: 21102443
PDF is available here.
PDF is available here.
Amplification of unknown RNAs and RNA mixtures based on unique restriction enzyme cleavage in vitro.
Abstract
We have developed a novel method to amplify target RNA. The amplification procedure was carried out by sequential RT-PCR, effective separation, restriction enzymatic cleavage of cDNA strand, and run-off transcription in vitro of target RNA from its cDNA. Introduction of a unique stem-loop linker int...
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PMID: 21106769
PDF is available here.
PDF is available here.
Abstract
We use standard molecular dynamics and replica exchange molecular dynamics to take a series of tests on the RNA stability under different combinations of Amber force field parameters (ff98, ff99 and ff99bsc0) and the general Born implicit solvent models (igb1, igb2 and igb5). It is found that only f...
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PMID: 20919758
PDF is available here.
PDF is available here.
British Journal of Nutrition (104)12 2010
Abstract
We hypothesised that unheated phaseolin influences mucin flow and gene expression, and that phaseolin heat treatment reverses these effects. The hypothesis was tested using a control diet containing casein as the sole protein source and three other diets with casein being replaced by 33 and 67 % of...
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PMID: 20673379
PDF is available here.
PDF is available here.
Journal of Insect Physiology (56)12 2010
Abstract
We report that the integrin β1 subunit from hemocytes of Ostrinia furnacalis (Ofint β1) plays an important role in regulating the spreading of plasmatocytes. The full length cDNA sequence (4477 bp) of Ofint β1 was cloned from hemocytes. Phylogenetic analysis showed that Ofint β1 belonged to the...
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PMID: 20708011
PDF is available here.
PDF is available here.
Biomedical Chromatography (24)12 2010
Abstract
Silica-based columns are largely used in RNA purification, allowing fast extractions and good yields of high quality nucleic acid, but their major limitation is the high cost. The reuse of such columns, although desirable, is not recommended because of residual amounts of material from the previous...
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PMID: 21117284
PDF is available here.
PDF is available here.
Anesthesiology (113)6 2010
Abstract
We found gene expression of the oxygen-sensing pathway, heme oxygenase 2, and the K channels TASK (TWIK-related acid sensitive K channel)-1 and BK (large-conductance potassium channel). In addition, we show the expression of critical receptor subunits such as γ-aminobutyric acid A (α2, β3, and γ...
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PMID: 20980909
PDF is available here.
PDF is available here.
Abstract
We recently reported that expression and induction of metallothionein, a reactive oxygen species scavenger, is reduced in BAV ascending aortic aneurysms relative to nonaneurysmal patients.
Tissue and primary SMCs from patients with and without thoracic aortic aneurysms and metallothionein-null and w...
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PMID: 21095332
PDF is available here.
PDF is available here.
Chemical Communications (46)40 2010
Abstract
Photo-induced artificial RNA editing was demonstrated using photo-reactive oligonucleotides containing 3-cyanovinylcarbazole nucleoside. This non-enzymatic and sequence-specific methodology will make a major contribution to the elucidation of RNA functions including non-coding RNAs and to the develo...
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PMID: 20848024
PDF is available here.
PDF is available here.
EMBO Reports (11)11 2010
Abstract
We have determined the crystal structure of the zinc-finger domain of zebrafish Nanos, for the first time revealing that Nanos adopts a novel zinc-finger structure. In addition, Nanos has a conserved basic surface that is directly involved in RNA binding. Our results provide the structural basis for...
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PMID: 20948543
PDF is available here.
PDF is available here.
EMBO Reports (11)11 2010
Abstract
Abstract
Abstract
We constructed an empirical fitness landscape for a catalytic RNA by combining next-generation sequencing, computational analysis, and "serial depletion," an in vitro selection protocol. By determining the reaction rate constant for every point mutant of a catalytic RNA, we demonstrated that abundan...
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PMID: 20947767
PDF is available here.
PDF is available here.
Abstract
We have engineered an expression vector, p1D4-hrGFP II, in order to efficiently express visual pigments in mammalian cell culture. This expression vector is based on pIRES-hrGFP II (Stratagene), with the addition of a C-terminal 1D4 epitope tag for immunoblotting and immunoaffinity purification. Thi...
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PMID: 20627111
PDF is available here.
PDF is available here.
Abstract
We have established a method for analyzing base-methylated cytosines in RNA using bisulfite sequencing. Treatment of RNA with bisulfite causes the chemical deamination of nonmethylated cytosines to uracil, while methylated cytosines remain unaffected. cDNA synthesis followed by polymerase chain reac...
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PMID: 20889702
PDF is available here.
PDF is available here.
Abstract
We have previously shown that deficits in vocal learning occur in zebra finches after reduction of FoxP2 in Area X, a striatal nucleus involved in song acquisition. We recently showed that FoxP2 is expressed in newly generated spiny neurons (SN) in adult Area X as well as in the ventricular zone (VZ...
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PMID: 20528955
PDF is available here.
PDF is available here.
Abstract
The Modern Challenges in Therapeutic Protein Production conference hosted 44 delegates from the UK and Germany and was organized by EuroSciCon. The aim of the meeting was to look at the challenges that currently face the production of therapeutic proteins and to attempt to demystify some of the nove...
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PMID: 20973635
PDF is available here.
PDF is available here.
Abstract
The initial evaluation of the subfertile male includes a through history and physical examination, semen analyses, and hormonal evaluation. However, a normal spermiogram does not necessarily correlate with fertility potential, because it does not assess sperm function. For this reason, specialized s...
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PMID: 20887631
PDF is available here.
PDF is available here.
Abstract
We report that DC derived from transgenic mice, which solely express a naturally occurring CYLD isoform (CYLD(ex7/8)), display a higher content of nuclear RelB and express elevated levels of NF-κB family members as well as of known NF-κB-target genes comprising costimulatory molecules and pro-infl...
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PMID: 20836156
PDF is available here.
PDF is available here.
Abstract
We tested the hypothesis that islet isolation and transplantation activate innate immunity through TLR expressed on islets. Murine islets constitutively express TLR2 and TLR4, and TLR activation with peptidoglycan or LPS upregulates islet production of cytokines and chemokines. Following transplanta...
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PMID: 20809521
PDF is available here.
PDF is available here.
Food and Chemical Toxicology (48)10 2010
Abstract
Reactive oxygen species (ROS) and oxidative damage are the most important factors in cisplatin-induced acute renal failure. This study examined the protective effects of crocin against cisplatin-induced renal oxidative stress in rat. Animals were divided into five groups (n=6). Group 1 received norm...
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PMID: 20600529
PDF is available here.
PDF is available here.
Brain research (1352) 2010
Abstract
Our study may provide the scientific benefit of social attachment in our daily life....
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PMID: 20643112
PDF is available here.
PDF is available here.
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (68) 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract