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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Iodoacetamide (0):
Recent article
Iodoacetamide
Abstract
We have developed the first example of a one-donor/three-acceptor multicolor time-resolved fluorescence energy transfer (TR-FRET) system, and we have exemplified its use by monitoring a ligand-regulated protein-protein exchange process in a four-component biological system. By careful quantification...
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PMID: 20230029
PDF is available here.
PDF is available here.
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Mikrobiologiia (79)1 2010
Abstract
Abstract
Electron transfer dissociation (ETD) has proven to be a promising new ion activation method for proteomics applications due to its ability to generate c- and z-type fragment ions in comparison to the y- and b-type ions produced upon the more conventional collisional activation of peptides. However,...
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PMID: 19722535
PDF is available here.
PDF is available here.
Abstract
We examined the inhibitory effects of protein tyrosine phosphatase (PTPase) inhibitors, including sodium orthovanadate (SOV), ammonium molybdate (AM), and iodoacetamide (IA), on cell growth, accumulation of astaxanthin, and PTPase activity in the photosynthetic algae Haematococcus lacustris. PTPase...
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PMID: 19809248
PDF is available here.
PDF is available here.
Abstract
134 fungal cultures isolated from different soil samples were screened for lovastatin production. Of these, 38 isolates produced different levels of lovastatin. An Aspergillus terreus strain GD13, producing 190 mg/l of lovastatin was selected and subjected to a rational mutation-selection programme...
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PMID: 19621768
PDF is available here.
PDF is available here.
Abstract
The present study deals with the determination of hydrodynamic size of DNA/cationic gemini surfactant complex in sodium bromide solution using the dynamic light scattering method. Cationic gemini surfactants with polymethylene spacer of variable length were used for the interaction with DNA. The sca...
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PMID: 19592712
PDF is available here.
PDF is available here.
Nature Methods (6)5 2009
Abstract
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. P...
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PMID: 19377485
PDF is available here.
PDF is available here.
Biochemical Journal (419)2 2009
Abstract
We hypothesized that mitochondrial protein modification may also be important in Keap1/Nrf-2 signal transduction, leading to HO-1 up-regulation. In order to determine the role of mitochondrial protein thiol modification in HO-1 induction, we used the mitochondrial-targeted thiol-reactive compound IB...
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PMID: 19161347
PDF is available here.
PDF is available here.
Abstract
We have concluded that p53 is subject to structural redox modulation and that this modulation affects the functional ability of the protein to bind to DNA, to cause cell cycle arrest, and to trigger apoptosis....
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PMID: 19253049
PDF is available here.
PDF is available here.
Abstract
We describe a suite of methods to measure nuclear redox state, which include a redox Western blot technique to quantify the redox state of Trxl, a biotinylated iodoacetamide (BIAM) method for thioredoxin reductase-1 (TrxR1), GSH redox measurement using total protein S-glutathionylation, and a redox...
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PMID: 18951192
PDF is available here.
PDF is available here.
Abstract
Our study aimed to investigate the vagal afferents activity and the gastro-duodenal slow wave response to the mild gastric mucosa inflammation in rats. The gastric mucosal inflammation was induced by addition iodoacetamide to drinking water for 5 days. The gastro-duodenal slow wave, vagal nerve reco...
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PMID: 19212005
PDF is available here.
PDF is available here.
Biophysical Journal (95)6 2008
Abstract
Our results showed that merocyanine states, with a larger dipole moment, interact with the motor's extended conformation stronger than with the compact conformation by 1.6 x 10(-21) J/molecule. The interaction sites are near the cytoplasmic side of the motor protein....
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PMID: 18556757
PDF is available here.
PDF is available here.
Abstract
We describe here for the first time a simple, less time consuming, novel method for producing a non-toxic toxoid that is structurally and antigenically more similar to the native toxin. Toxin is chemically inactivated by alkylation with iodoacetamide in the presence of reversibly denaturing conditio...
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PMID: 18571196
PDF is available here.
PDF is available here.
Abstract
We have analysed the effect of Cu2+ (0.1 to 50 mM) on the kinetics of MTX oxidative folding and found that it dramatically speeds up the formation of the four-disulphide bridged, native-like, MTX (maximal production within 30 minutes instead of > 60 hours). This catalysing effect of Cu2+ was found t...
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PMID: 18533728
PDF is available here.
PDF is available here.
Abstract
Findings indicative of UC were seen in the combined treatment (IA + B) as well as the IA continued treatment groups: the animals showed slow rate of increase in body weight, diarrhea, bloody stools, high colonic ulcer score, as well as histological alterations characteristic of UC, with an extensive...
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PMID: 18609687
PDF is available here.
PDF is available here.
Abstract
Gastritis, an inflammatory state in gastric mucosa, can be induced experimentally in various ways. The present study considered the iodoacetamide model (Iodo). Omega-3 fatty acids (fish oil), black seed oil, and curcuminoids (natural products) in addition to omeprazole (synthetic proton-pump inhibit...
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PMID: 18368490
PDF is available here.
PDF is available here.
Nature Methods (5)6 2008
Abstract
Abstract
These results suggest that increases in radial gradients of O2 potentially promote cell death through the reverse action of F1Fo in mitochondria located at the anoxic cell core. However, in the intact cardiomyocyte, the CK-mediated energy flux from the subsarcolemmal space may sustain DeltaPsim at t...
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PMID: 18424639
PDF is available here.
PDF is available here.
Gastroenterology (134)7 2008
Abstract
These studies demonstrate that gastric irritation in the neonatal period can result in chronic gastric hypersensitivity and gastric motor dysfunction in adults even in the absence of significant detectable gastric pathology. Our results offer insight into the pathogenesis of chronic functional dyspe...
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PMID: 18448102
PDF is available here.
PDF is available here.
Abstract
We demonstrated that both water-soluble and insoluble nickel compounds induce histone ubiquitination (uH2A and uH2B) in a variety of cell lines. Investigations of the mechanism by which nickel increases histone ubiquitination in cells reveal that nickel does not affect cellular levels of the substra...
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PMID: 18279901
PDF is available here.
PDF is available here.
Analytical Biochemistry (375)2 2008
Abstract
Cysteine reactivity in enzymes is imparted to a large extent by the stabilization of the deprotonated form of the reduced cysteine (i.e., the thiolate) within the active site. Although this is likely to be an important chemical attribute of many thiol-based enzymes, including cysteine-dependent pero...
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PMID: 18162165
PDF is available here.
PDF is available here.
Analytical Biochemistry (374)2 2008
Abstract
We present here an optimized and cost-effective approach to saturation fluorescence labeling of protein thiols for proteomic analysis. We investigated a number of conditions and reagent concentrations, including the disulfide reducing agent tris(2-carboxyethyl)phosphine (TCEP), pH, incubation time,...
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PMID: 18191033
PDF is available here.
PDF is available here.
Abstract
We show that 3CLpro has mechanistic features common and disparate to the archetypical proteases papain and chymotrypsin. The kinetic mechanism for 3CLpro-mediated ester hydrolysis, including the individual rate constants, is consistent with a simple double displacement mechanism. The pre-steady-stat...
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PMID: 18237196
PDF is available here.
PDF is available here.
Abstract
Abstract
These results indicate that failure of NO to inhibit migration in VSMC exposed to HG is due to oxidation of the SERCA reactive cysteine-674....
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PMID: 18164028
PDF is available here.
PDF is available here.
Abstract
We developed a fluorescent biosensor (Mero-S100A4) that reports on the Ca2+-bound, activated form of S100A4. Direct attachment of a novel solvatochromatic reporter dye to S100A4 results in a sensor that, upon activation, undergoes a 3-fold enhancement in fluorescence, thus providing a sensitive assa...
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PMID: 18154362
PDF is available here.
PDF is available here.
Neuroscience (150)3 2007
Abstract
We examined whether experimental gastritis modifies anxiety, stress coping and circulating corticosterone in male and female Him:OF1 mice. Gastritis was induced by adding iodoacetamide (0.1%) to the drinking water for at least 7 days. Inflammation was assessed by gastric histology and myeloperoxidas...
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PMID: 17945426
PDF is available here.
PDF is available here.
Helicobacter (12)6 2007
Abstract
The kinetics and properties of H. pylori phenylphosphonate transport were characterized, and the data suggested a carrier-mediated transport mechanism....
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PMID: 18001401
PDF is available here.
PDF is available here.
Abstract
We show that the BIA-modified C151 tryptic peptide is reproducibly detected by our method. We also investigated the key differences in the methods that have been used to prepare the protein for modification by BIA. Removal of the reducing agent from Keap1 before the addition of BIA did not significa...
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PMID: 17935299
PDF is available here.
PDF is available here.
Abstract
We monitored, in real time, the cytosolic ATP level in luciferase-expressing HeLa cells. A mitochondrial uncoupler or a respiration chain inhibitor was found to decrease cytosolic ATP by about 50%. However, even when mitochondrial ATP synthesis was suppressed, STS induced a profound elevation of int...
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PMID: 17897621
PDF is available here.
PDF is available here.
Analytical Biochemistry (370)1 2007
Abstract
We report for the first time on the copper (Cu(2+)) binding characteristics of the far-red fluorescent protein, HcRed, and its application in the development of a reagentless sensing system for copper. The far-red emission of HcRed (lambda(max) = 645 nm) where background cellular fluorescence is low...
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PMID: 17599800
PDF is available here.
PDF is available here.
Abstract
Cysteine thiol modifications are increasingly recognized to occur under both physiological and pathophysiological conditions, making their accurate detection, identification, and quantification of growing importance. Among free cysteines, the bulk of modifications occurs on a subset of cysteines tha...
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PMID: 17854705
PDF is available here.
PDF is available here.
Journal of Neuroscience (27)42 2007
Abstract
We show that formaldehyde activates the ion channel TRPA1 and that TRPA1-deficient mice exhibit dramatically reduced formaldehyde-induced pain responses. 4-Hydroxynonenal, a reactive chemical produced endogenously during oxidative stress, and other related aldehydes also activate TRPA1 in vitro. Fur...
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PMID: 17942735
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)41 2007
Abstract
We have previously shown that GCP60, a Golgi resident protein, interacts weakly with the golgin-160 head domain but has a strong interaction with one of the caspase-generated golgin-160 fragments (residues 140-311). This preferential interaction increases the Golgi retention of the golgin-160 fragme...
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PMID: 17711851
PDF is available here.
PDF is available here.
Abstract
Substrate specificity within the family of enzymes designated as homoserine transsuccinylases is variable, with some organisms utilizing succinyl-CoA and other organisms utilizing acetyl-CoA. In this study it is shown that the enzyme from Bacillus cereus uses acetyl-CoA as its acyl donor, but its ca...
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PMID: 17662245
PDF is available here.
PDF is available here.
European Journal of Pharmacology (570)1-3 2007
Abstract
The effects of the alkylaminoalkanethiosulfuric acids (AAATs), new schistosomicidal drugs, on Schistosoma mansoni ATP diphosphohydrolase isoforms, members of the NTPDase family, were analyzed. Pre-incubation of worm adult tegument with AAATs derivatives, with small apolar alkyl groups and an apolar...
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PMID: 17588561
PDF is available here.
PDF is available here.
Biochemistry (Washington) (46)35 2007
Abstract
Factor XIII can be activated proteolytically by thrombin cleavage of the activation peptide or non-proteolytically by exposure to 50 mM Ca2+. The resultant transglutaminase cross-links Q and K residues within the noncovalently associated fibrin clot. Hydrogen deuterium exchange coupl...
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PMID: 17691819
PDF is available here.
PDF is available here.
Abstract
We demonstrate how to optimize this process with chemical synthesis. The fluorescence of fluorescein-labeled model protein, bovine pancreatic ribonuclease (RNase A), decreases upon binding to its cognate inhibitor protein (RI). Free and RI-bound fluorescein-RNase A have pKa values of 6.35 and 6.70,...
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PMID: 17672523
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)35 2007
Abstract
We studied the processing of the toxins. Dithiothreitol and beta-mercaptoethanol induced auto-cleavage of purified native toxin A and toxin B into approximately 250/210- and approximately 63-kDa fragments. The 63-kDa fragment was identified by mass spectrometric analysis as the N-terminal glucosyltr...
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PMID: 17591770
PDF is available here.
PDF is available here.
Toxicology In Vitro (21)5 2007
Abstract
We tested the hypothesis that cytoprotection following ER stress preconditioning is a common property of renal cell lines (LLC-PK1 (pig), NRK-52E (rat), HEK293 (human), MDCK (dog)) and extends to clinically relevant nephrotoxins. ER stress inducers (tunicamycin, thapsigargin and oxidized dithiothrei...
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PMID: 17416481
PDF is available here.
PDF is available here.
Toxicology In Vitro (21)5 2007
Abstract
Precision-cut tissue slices mimic specific organ toxicity because normal cellular heterogeneity and organ architecture are retained. To optimize the use of the smaller tissues of the mouse and to establish easy assays for tissue viability, a tissue chip based system was used to generate large number...
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PMID: 17376647
PDF is available here.
PDF is available here.
Abstract
We attempted to avoid using iodoacetamide (IAM) to stabilize the required oxidizing thiol redox potential, since previous IAM pretreatments prevented glucose utilization apparently by inactivating glyceraldehyde 3-phosphate dehydrogenase (G-3PDH). Instead, the glutathione reductase (Gor)-mediated di...
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PMID: 17154312
PDF is available here.
PDF is available here.
ChemBioChem (8)9 2007
Abstract
Abstract
We purified the galectin using a combination of ammonium sulphate fractionation and affinity chromatography and the homogeneity was determined by both native polyacrylamide gel electrophoresis (PAGE) and denaturing SDS-PAGE. The molecular weight of the galectin as determined by SDS-PAGE under reduci...
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PMID: 17218122
PDF is available here.
PDF is available here.
Abstract
We have applied liquid chromatography-tandem mass spectrometry (LC-MS-MS) to identify protein targets of the biotin-tagged model electrophiles 1-biotinamido-4-(4'-[maleimidoethylcyclohexane]-carboxamido)butane (BMCC) and N-iodoacetyl-N-biotinylhexylenediamine (IAB) in human liver microsomes. The bio...
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PMID: 17480101
PDF is available here.
PDF is available here.
Abstract
We describe the use of mass spectrometry to show that a single equivalent of raloxifene is bound to the intact P450 apoprotein. Furthermore, mass analysis of peptides following digestion with proteinase K revealed that the covalently bound drug is localized to residue Cys239. A mass shift of 471 Da...
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PMID: 17497897
PDF is available here.
PDF is available here.
Journal of Virology (81)12 2007
Abstract
Sindbis virus is a single-stranded positive-sense RNA virus. It is composed of 240 copies of three structural proteins: E1, E2, and capsid. These proteins form a mature virus particle composed of two nested T=4 icosahedral shells. A complex network of disulfide bonds in the E1 and E2 glycoproteins i...
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PMID: 17409163
PDF is available here.
PDF is available here.
Neuroscience (145)3 2007
Abstract
We conclude that the gastritis-evoked increase in the gastric acid-evoked c-Fos expression in the NTS is related to disruption of the gastric mucosal barrier, mucosal inflammation, mucosal acid influx and enhanced activation of the afferent stomach-NTS axis....
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PMID: 17303342
PDF is available here.
PDF is available here.
Biochemistry (Washington) (46)10 2007
Abstract
Homoserine transsuccinylase catalyzes the succinylation of homoserine in several bacterial species, the first unique step in methionine biosynthesis in these organisms. The enzyme from Escherichia coli is reported to be a dimer and uses a ping-pong catalytic mechanism involving transfer of succinate...
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PMID: 17302437
PDF is available here.
PDF is available here.
Abstract
Inflammatory bowel diseases are characterized by dysregulated immune response to the normal microflora and structural and functional changes of the enteric nervous system which occur in inflamed as well as non-inflamed areas of the bowel. This study describes the changes in the expression of nerve g...
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PMID: 17517520
PDF is available here.
PDF is available here.
Abstract
The uptake and transepithelial transport of the three main constituents macrocarpal A (M-A), macrocarpal B (M-B), and cypellocarpa C (Cy-C) from the fruits of Eucalyptus globulus Labill. were investigated. Monolayers of the human intestinal epithelial cancer cell line Caco-2 were incubated with M-A,...
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PMID: 17118653
PDF is available here.
PDF is available here.
Abstract
We report here that iodoacetamide reacts with the N-terminal NH2 and the C-terminal carboxylic acid groups, in addition to the peripheral residues bearing protic functional groups. If sufficient reaction time is given, the N-terminal NH2 group is readily dialkylated by iodoacetamide. In fact, the N-...
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PMID: 17206739
PDF is available here.
PDF is available here.
Abstract
In the present study, HPLC-ESI-IT (ion-trap) MS was used for carboxyterminal (C-terminal) amino acid sequence confirmation of intact recombinant Hirudin Variant 3 (HV3) and alkylated HV3. The C-terminal amino acid sequence of HV3 was determined by the use of carboxypeptidase P (CPP), and by the comb...
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PMID: 17141443
PDF is available here.
PDF is available here.
Abstract
5-Bromo-2[(2-iodoacetyl)amino]benzenesulfonic acid (AIBSA), a reagent for modification of free of cysteine thiol groups in proteins and peptides, was synthesized. Rate constants of its interaction with thiol groups were determined. The presence of a bromine atom allows an easy identification of the...
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PMID: 18173121
PDF is available here.
PDF is available here.
Abstract
Abstract
Our method eliminates fundamental problems in other existing isotope-tagging methods requiring liquid chromatography and MS/MS, such as isotope effects, fragmentation, and solubility. It is also anticipated to be more practical and accessible than those LC-dependent methods....
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PMID: 17049249
PDF is available here.
PDF is available here.
Abstract
D-labeled and -unlabeled N-beta-naphthyliodoacetamides have been synthesized for specific modification of the sulfhydryl groups of cysteine residues in proteins or peptides, and have been applied to quantitative analysis of several peptides. A combination of these reagents, coupled with mass spectro...
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PMID: 16979336
PDF is available here.
PDF is available here.
Abstract
We show that heterologously expressed and purified precursor peptides HalA1 and HalA2 are processed by the purified modification enzymes HalM1 and HalM2 in an in vitro reconstitution of the biosynthesis of a two-component lantibiotic. The activity of each HalM enzyme is substrate-specific, and the a...
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PMID: 17085596
PDF is available here.
PDF is available here.
Toxicological Sciences (94)1 2006
Abstract
We explored the link between ER stress, calpain, and cell death in renal cell injury due to model RCTs (iodoacetamide, menadione, tert-butyl hydroperoxide) and ER stress inducers (tunicamycin [TUN], thapsigargin [THAPS]). The calpain inhibitor, PD150606, significantly reduced the RCT and TUN-induced...
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PMID: 16920763
PDF is available here.
PDF is available here.
Canadian Journal of Physiology and Pharmacology/Revue Canadienne de Physiologie et Pharmacologie (84)11 2006
Abstract
We conclude that colitis can induce significant functional abnormalities in distant noninflamed small bowel regions....
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PMID: 17218984
PDF is available here.
PDF is available here.