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Organisms, Genetically Modified (1):
Recent article
Organisms, Genetically Modified
Proteomics (11)12 2011
Abstract
Small-colony variants (SCVs) of Staphylococcus aureus represent a slow-growing subpopulation causing chronic and relapsing infections due to their physiological adaptation on an intracellular lifestyle. In this first proteomic study on physiological changes associated with a natural,...
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PMID: 21595038
PDF is available here.
PDF is available here.
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Clinical Cancer Research (17)9 2011
Abstract
This study demonstrated that oncolytic Ads containing the sequences complementary to normal cell-specific miRNAs showed significantly improved safety profiles without altering tumor cell lysis activity....
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PMID: 21346145
PDF is available here.
PDF is available here.
Abstract
We show that helicase loading by ORC is inhibited by two distinct CDK-dependent mechanisms. Independent of phosphorylation, binding of CDK to an "RXL" cyclin-binding motif in Orc6 sterically reduces the initial recruitment of the Cdt1/Mcm2-7 complex to ORC. CDK phosphorylation of Orc2 and Orc6 inhib...
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PMID: 21289063
PDF is available here.
PDF is available here.
Abstract
We found that either limitation or starvation for any of the three nutrients elicits all of the physiological properties associated with quiescence, such as enhanced cell wall integrity and resistance to heat shock and oxidative stress. Moreover, the starvations result in a common transcriptional pr...
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PMID: 21289062
PDF is available here.
PDF is available here.
Abstract
We report here that the pairing of the sRNA RyhB to the target mRNA sodB initiates mRNA degradation even in the absence of translation on the mRNA target. Remarkably, even though it pairs at the RBS, the sRNA RyhB induces mRNA cleavage in vivo at a distal site located >350 nucleotides (nt) downstrea...
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PMID: 21289064
PDF is available here.
PDF is available here.
Abstract
We analyzed the functional profile of CD8+ T-cell responses directed against autologous transmitted/founder HIV-1 isolates during acute and early infection, and examined whether multifunctionality is required for selection of virus escape mutations. Seven anti-retroviral therapy-naïve subjects were...
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PMID: 21347345
PDF is available here.
PDF is available here.
Abstract
We sought to confirm the regulatory role of BosR in a second virulent strain (strain 297) of Bb. Indeed, BosR displayed the same influence over lipoprotein expression and mammalian infectivity for strain Bb 297 that were previously noted for Bb strain B31. We subsequently found that recombinant BosR...
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PMID: 21347346
PDF is available here.
PDF is available here.
Abstract
We describe serine phosphorylation of the DegS sensing domain, which stimulates its kinase activity. We demonstrate that DegS phosphorylation can be carried out by at least two B. subtilis Hanks-type kinases in vitro, and this stimulates the phosphate transfer towards DegU. The consequences of this...
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PMID: 21304896
PDF is available here.
PDF is available here.
Abstract
The enzyme glutamine: fructose-6-phosphate aminotransferase (GFAT), also known as glucosamine synthase (GlmS), catalyzes the formation of glucosamine-6-phosphate from fructose-6-phosphate and is the first and rate-limiting enzyme of the hexosamine biosynthetic pathway. For the first time, the GFAT ge...
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PMID: 21340036
PDF is available here.
PDF is available here.
Abstract
We have shown that WalKR acts as the master controller of peptidoglycan metabolism, yet none of the identified regulon genes explain its requirement for cell viability. Transmission electron micrographs revealed cell wall thickening and aberrant division septa in the absence of WalKR, suggesting its...
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PMID: 21386961
PDF is available here.
PDF is available here.
Abstract
We systematically queried the Saccharomyces cerevisiae proteome for Prp19 WD40 domain interaction partners by two-hybrid analysis. We report that in addition to S. cerevisiae Cwc2, the splicing factor Prp17 binds directly to the Prp19 WD40 domain in a 1:1 ratio. Prp17 binds simultaneously with Cwc2...
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PMID: 21386897
PDF is available here.
PDF is available here.
Abstract
We developed a transgenic Lactobacillus acidophilus strain that constitutively expresses IFN-β (La-IFN-β). While pretreatment of mice with control Lactobacillus (La-EV) provided slight protective benefits, La-IFN-β increased sensitivity to DSS. Analysis showed colitic mice pretreated with La-IFN-...
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PMID: 21365015
PDF is available here.
PDF is available here.
Scientific American (303)5 2010
Abstract
Scientific American (303)5 2010
Abstract
Clinical Cancer Research (16)21 2010
Abstract
To determine the maximum tolerated dose (MTD), toxicity spectrum, clinical activity, and biological effects of the tropism-modified, infectivity-enhanced conditionally replicative adenovirus (CRAd), Ad5-Δ24-Arg-Gly-Asp (RGD), in patients with malignant gynecologic diseases.
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PMID: 20978148
PDF is available here.
PDF is available here.
Microbial Ecology (60)3 2010
Abstract
We have investigated the nature of the association between Asaia and the major Afro-tropical malaria vector Anopheles gambiae. We have isolated Asaia from different wild and laboratory reared colonies of A. gambiae, and it was detected by PCR in all the developmental stages of the mosquito and in al...
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PMID: 20571792
PDF is available here.
PDF is available here.
Abstract
Spiders and silkworms generate silk protein fibers that embody strength and beauty. Orb webs are fascinating feats of bioengineering in nature, displaying magnificent architectures while providing essential survival utility for spiders. The unusual combination of high strength and extensibility is a...
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PMID: 20671180
PDF is available here.
PDF is available here.
Abstract
We sought to assess the versatility of transgenic algae as a recombinant protein production platform. To do this, we tested whether the algal chloroplast could support the expression of a diverse set of current or potential human therapeutic proteins. Of the seven proteins chosen, >50% expressed at...
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PMID: 20230484
PDF is available here.
PDF is available here.
Trends in Parasitology (26)7 2010
Abstract
Genetic engineering provides an ingenious method of attenuating Plasmodium falciparum parasites for next generation vaccines. A novel approach stimulates new optimism in the struggle to eliminate the burden of malaria.
Copyright 2010 Elsevier Ltd. All rights reserved.
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PMID: 20537954
PDF is available here.
PDF is available here.
Abstract
We have developed an inexpensive nonradioactive high-throughput screening system to identify new ACC inhibitors. The screen uses yeast gene-replacement strains depending for growth on cloned human ACC1 and ACC2. In "proof of concept" experiments, growth of such strains was inhibited by compounds kno...
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PMID: 20439761
PDF is available here.
PDF is available here.
Molecular Biotechnology (44)3 2010
Abstract
GM risk assessments (GMRAs) play an important role in the decision-making process surrounding the regulation, notification and permission to handle Genetically Modified Organisms (GMOs). Ultimately the role of each GMRA will be able to ensure the safe handling and containment of the GMO; and to asse...
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PMID: 20087690
PDF is available here.
PDF is available here.
Development (137)6 2010
Abstract
We investigated the molecular mechanisms underlying the prominent vascular abnormalities that occur in the absence of β1 integrins. Because of the early embryonic lethality of knockout mice, we studied endothelial cell and vessel development in β1-integrin-deficient murine embryonic stem cells...
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PMID: 20179098
PDF is available here.
PDF is available here.
Abstract
Our results demonstrate that pulmonary infection with C. neoformans strain H99gamma results in the induction of classically activated macrophages and promotes fungal clearance. These studies indicate that phenotype, as opposed to quantity, of infiltrating macrophages correlates with protection again...
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PMID: 20056835
PDF is available here.
PDF is available here.
EMBO Journal (29)3 2010
Abstract
We report that, like the CE component Zip1, Saccharomyces cerevisiae axial-element structural protein, Red1, can bind small ubiquitin-like modifier (SUMO) polymeric chains. The Red1-SUMO chain interaction is dispensable for the initiation of meiotic DNA recombination, but it is essential for Tel1- a...
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PMID: 19959993
PDF is available here.
PDF is available here.
Abstract
It has been demonstrated that the duplicated carbonic anhydrase is induced by salt in the Dunaliella salina (D. salina) and duplicated carbonic anhydrase 1 (DCA1) is a member of carbonic anhydrase family. The purpose of this study was to identify whether both the DCA1 gene and its promoter from D. s...
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PMID: 19823944
PDF is available here.
PDF is available here.
Clinical Cancer Research (16)2 2010
Abstract
These data suggest that the novel oncolytic mutant AdDeltaDelta is a promising candidate for targeting of solid tumors specifically in combination with chemotherapeutics....
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PMID: 20068104
PDF is available here.
PDF is available here.
Abstract
We summarize micromanipulation methods for measuring the physical plasticity of the nucleus, with recent studies documenting the extreme flexibility of human embryonic stem cells and the rigidification in model aging of progerin-type nuclei. Lamin-A/C is a common molecular factor, and methods are pr...
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PMID: 20816236
PDF is available here.
PDF is available here.
Abstract
Eukaryotic microalgae have recently gained particular interest as bioreactors because they provide attractive alternatives to bacterial, yeast, plant and other cell-based systems currently in use. Over the last years there has been considerable progress in genetic engineering technologies for algae....
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PMID: 21520709
PDF is available here.
PDF is available here.
Abstract
We report that MoAtg4, an Atg4 homologue in Magnaporthe oryzae, controls cell differentiation and pathogenicity by interacting with MoAtg8, an autophagic protein essential for autophagic cell death and pathogenicity. Yeast complementation assay revealed that MoATG4 can functionally complement the de...
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PMID: 19923912
PDF is available here.
PDF is available here.
Abstract
We found that PpAtg6 is required for all autophagic pathways that have been identified in the yeast Pichia pastoris, as well as for the carboxypeptidase Y (PpCPY) vacuolar protein sorting pathway. We localized PpAtg6 to the phagophore assembly site (PAS) and observed its continued presence at that s...
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PMID: 19946209
PDF is available here.
PDF is available here.
Autophagy dysfunction and ubiquitin-positive protein aggregates in Dictyostelium cells lacking Vmp1.
Abstract
We now report several lines of evidence suggesting a defect in autophagy in Dictyostelium cells lacking Vmp1 (vacuole membrane protein 1), an endoplasmic reticulum (ER)-resident protein involved in pathological processes such as cancer and pancreatitis. vmp1- null cells are unable to survive starvat...
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PMID: 20009561
PDF is available here.
PDF is available here.
Abstract
We present evidence that the pore-forming toxin alpha-hemolysin (Hla) is a S. aureus secreted factor which participates in the activation of the autophagic pathway. In addition, our results indicate that although the toxin elicits an autophagic response this pathway is dysfunctional as indicated by...
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PMID: 20110774
PDF is available here.
PDF is available here.
Abstract
The completion of the WHO Schistosoma Genome Project in 2008, although not fully annotated, provides a golden opportunity to actively pursue fundamental research on the parasites genome. This analysis will aid identification of targets for drugs, vaccines and markers for diagnostic t...
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PMID: 21415884
PDF is available here.
PDF is available here.
Abstract
We will describe a technique that can be used to identify heterozygous mutations in dosage-sensitive genes that mediate genomic stability by performing genome-wide screens in yeast....
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PMID: 20721738
PDF is available here.
PDF is available here.
Gene Expression (15)2 2010
Abstract
We hypothesized that downregulation of Dd-Grp94 protein would affect Dictyostelium cell structure, growth, and development. We found that Dd-grp94 RNAi recombinants exhibited reduced growth rate, cell size, and a subtle change in cell motility compared to the parental cells. The recombinants also ex...
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PMID: 21526718
PDF is available here.
PDF is available here.
BMC Biology (8)1 2010
Abstract
We survey mechanisms that enable a phagocyte to remodel actin organization in response to particles of complex shape.
Using particles that consist of two lobes separated by a neck, we found that Dictyostelium cells transmit signals concerning the curvature of a surface to the actin s...
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PMID: 21190565
PDF is available here.
PDF is available here.
Biochemistry (New York) (74)13 2009
Abstract
Abstract (3/4) This review summarizes data about structural and functional organization of steroidogenic P450-dependent enzymatic systems. Problems of catalysis of steroid substrate transformation, special features of mitochondrial type P450scc topogenesis, and abilities of some microbial electron t...
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PMID: 20210705
PDF is available here.
PDF is available here.
PLoS Pathogens (5)10 2009
Abstract
We report that human THP-1 monocytic cells infected with a MYXV construct deleted for the M013L gene (vMyxM013-KO), in stark contrast to the parental MYXV, rapidly induce high levels of secreted pro-inflammatory cytokines like TNF, IL-6, and MCP-1, all of which are regulated by NF-kappaB. The induct...
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PMID: 19851467
PDF is available here.
PDF is available here.
Abstract
We focus here on building a stochastic kinetic model for the intracellular growth of vesicular stomatitis virus (VSV), a well-studied virus that encodes five genes. The essential network of VSV reactions creates challenges to stochastic simulation owing to (i) delayed reactions associated with trans...
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PMID: 19459014
PDF is available here.
PDF is available here.
Biochemical Journal (423)1 2009
Abstract
We use Saccharomyces cerevisiae mutants to examine the potential roles of inositol pyrophosphates in responding to cell damage caused by ROS (reactive oxygen species). Yeast lacking kcs1 [the S. cerevisiae IP6K (inositol hexakisphosphate kinase)] have greatly reduced IP7 (diphosphoinositol pentakisp...
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PMID: 19614566
PDF is available here.
PDF is available here.
Abstract
Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78%of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 16S rRNA sequence. The EPA content reache...
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PMID: 19809243
PDF is available here.
PDF is available here.
Abstract
In the late 1990s, the British anti-genetic engineering (GE) movement effectively closed Britain's market for genetically modified foods, while the U.S. anti-GE movement had a negligible impact. In seeking to explain the different outcomes of these similar social movements, the authors draw upon the...
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PMID: 19852188
PDF is available here.
PDF is available here.
Abstract
We have previously shown that ST3 is both necessary and sufficient for larval epithelial cell death in the remodeling intestine. To investigate the roles of ST3 in other organs and especially on different cell types, we have analyzed the effect of transgenic overexpression of ST3 in the tail of prem...
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PMID: 19429683
PDF is available here.
PDF is available here.
Anticancer Research (29)6 2009
Abstract
We report here a tumor-targeting strategy for pancreatic cancer using a modified auxotrophic strain of Salmonella typhimurium. The genetically-modified strain of S. typhimurium requires the amino acids arginine and leucine. These mutations preclude growth in normal tissue but do not reduce bacterial...
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PMID: 19528442
PDF is available here.
PDF is available here.
Abstract
We have previously shown by mathematical modeling that mass-release of harmless susceptible (toxin-sensitive) insects engineered with repressible female-specific lethality using release of insects carrying a dominant lethal ([RIDL] Oxitec Limited, United Kingdom) technology could substantially delay...
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PMID: 19449654
PDF is available here.
PDF is available here.
Abstract
We describe the osmotic response process of Dunaliella under salinity stress, including the changes of cell volume, intracellular ions concentration, intracellular glycerol concentration, and the expression of some salt-induced genes. Some specific proteins and enzymes can be induced by different sa...
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PMID: 19202552
PDF is available here.
PDF is available here.
Abstract
Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich and metal-binding functional proteins. Transgenic MT mushroom can be used as functional food additives, but its zinc-enriching ability has not been studied systemically until now. The zinc contents in mycelia of transgenic MT...
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PMID: 19455831
PDF is available here.
PDF is available here.
Abstract
These results lay the foundation for the further research on gene function of parasitic nematodes using C. elegans....
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PMID: 19621571
PDF is available here.
PDF is available here.
Abstract
We might witness another rise in patents on use of dehydration stress related gene sequences in creating environmental stress tolerant biological control agents for plant disease and insect pest management in agriculture. This review summarizes some of the recent published patents related to drought...
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PMID: 19149734
PDF is available here.
PDF is available here.
Journal of Bacteriology (191)1 2009
Abstract
We measure the effective axial diffusion coefficient of green fluorescent protein (D(GFP)) in the cytoplasm of E. coli cells as a function of (phi) for both plasmolyzed and adapted cells. For plasmolyzed cells, the median D(GFP) (D(GFP)(m)) decreases by a factor of 70 as (phi) increases from 0.16 to...
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PMID: 18952804
PDF is available here.
PDF is available here.
Abstract
Recent data on the synthesis and hydrolysis of flavin nucleotides in yeast and bacteria and the regulation of this process are summarized. Specific examples are provided and the prospects of the use of genetically modified microorganisms for the industrial manufacturing of flavin mononucleotide and...
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PMID: 19382698
PDF is available here.
PDF is available here.
FASEB Journal (23)1 2009
Abstract
We identified an evolutionary conserved GTPase-activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS-dependent UGA read-through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein...
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PMID: 18772345
PDF is available here.
PDF is available here.
Abstract
One of the most important principles of scientific endeavour is that the results be reproducible from lab to lab. Although research groups rarely redo the published experiments of their colleagues, research plans almost always rely on the work of someone else. The assumption is that if the same expe...
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PMID: 19799172
PDF is available here.
PDF is available here.
Abstract
Meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the evolutionary conserved Spo11 protein. Along the S. cerevisiae chromosomes, the DSB sites are not evenly distributed and the cleavage frequencies vary 10-100-fold from site to site. Herein are reviewed the methods use...
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PMID: 19799174
PDF is available here.
PDF is available here.
Abstract
Different sporulation and pre-sporulation regimens were compared for a number of commonly used laboratory strains of S. cerevisiae to define conditions that support high-efficiency sporulation.
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PMID: 19799173
PDF is available here.
PDF is available here.
Abstract
Commercialization of numerous genetically modified organisms (GMOs) has already been approved worldwide, and several additional GMOs are in the approval process. Many countries have adopted legislation to deal with GMO-related issues such as food safety, environmental concerns, and consumers' right...
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PMID: 20166592
PDF is available here.
PDF is available here.
Abstract
Abstract
We review functional genomics applications that require the mass production of cloned sequence repertoires, including ORFeomes and silencing tag collections. We discuss the techniques employed in large-scale cloning projects and we provide an up-to-date overview of the clone resources available for...
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PMID: 19588105
PDF is available here.
PDF is available here.
Abstract
We describe a quantitative genetic assay that uses exogenous site-specific recombination to monitor the level of homolog associations between two defined loci in living cells of budding yeast (5). We have used the Cre/loxP assay to genetically dissect nuclear architecture and meiotic homolog pairing...
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PMID: 19799176
PDF is available here.
PDF is available here.
Abstract
Interest in crossover interference in yeast has been spurred by the discovery and characterization of mutants that alter it as well as by the development and testing of models to explain it. This chapter describes methods for detecting and for measuring interference, with emphasis on those that expl...
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PMID: 19799175
PDF is available here.
PDF is available here.