Abstract
We produced metagenomic data by direct 454 pyrosequencing from bathypelagic plankton (1000 m depth) and bottom sediment of the Sea of Marmara, the gateway between the Eastern Mediterranean and the Black Seas. Data from small subunit ribosomal RNA (SSU rRNA) gene libraries and direct pyrosequencin...
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PMID: 20668488
PDF is available here.
Abstract
The plankton community plays an especially important role in the functioning of aquatic ecosystems and also in biogeochemical cycles. Since the beginning of marine research expeditions in the 1870 s, an enormous number of planktonic organisms have been described and studied. Plankton...
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PMID: 21104154
PDF is available here.
Abstract
We present a simple, novel one-pot assay for the analysis of antibiotic susceptibility of bacterial biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) clearly confirm that the co...
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PMID: 20938507
PDF is available here.
Abstract
Methylmercury (MeHg) and inorganic mercury (Hg(inorg)) were evaluated in the water of a Brazilian estuary, with two size classes of plankton and seven fish species of different feeding habits. Water partition coefficients (PCs) in microplankton were fourfold higher for MeHg than for Hg(inorg); and w...
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PMID: 20951393
PDF is available here.
Abstract
A significant amount of marine debris has accumulated in the North Pacific Central Gyre (NPCG). The effects on larger marine organisms have been documented through cases of entanglement and ingestion; however, little is known about the effects on lower trophic level marine organisms. This study is t...
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PMID: 21067782
PDF is available here.
Abstract
We combine model predictions with global assessments of MTL from catches, trawl surveys and fisheries stock assessments and find that catch MTL does not reliably predict changes in marine ecosystems. Instead, catch MTL trends often diverge from ecosystem MTL trends obtained from surveys and assessme...
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PMID: 21085178
PDF is available here.
Shibu Yooseph,
Kenneth H Nealson,
Douglas B Rusch,
John P McCrow,
Christopher L Dupont,
Maria Kim,
Justin Johnson,
Robert Montgomery,
Steve Ferriera,
Karen Beeson,
Shannon J Williamson,
Andrey Tovchigrechko,
Andrew E Allen,
Lisa A Zeigler,
Granger Sutton,
Eric Eisenstadt,
Yu-Hui Rogers,
Robert Friedman,
Marvin Frazier and
J Craig Venter
Abstract
We report the sequencing of 137 diverse marine isolates collected from around the world. We analysed these sequences, along with previously published marine prokaryotic genomes, in the context of marine metagenomic data, to gain insights into the ecology of the surface ocean prokaryotic picoplankton...
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PMID: 21048761
PDF is available here.
Abstract
We show how the information value of such ecological time-series increases through space and time in terms of their potential policy value. We also explore the co-evolution of these oceanic biological time-series with changing marine management drivers. Lessons learnt from reviewing these sequences...
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PMID: 20813425
PDF is available here.
Abstract
We showed that an Escherichia coli O157:H7 strain that was unable to form biofilm was retained in large numbers in dual-strain biofilms formed with an E. coli O-:H4 companion strain. In this study we tested additional companion strains for their ability to retain E. coli O157:H7 strain 0475s. Compan...
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PMID: 20367070
PDF is available here.
Philip J Motta,
Michael Maslanka,
Robert E Hueter,
Ray L Davis,
Rafael de la Parra,
Samantha L Mulvany,
Maria Laura Habegger,
James A Strother,
Kyle R Mara,
Jayne M Gardiner,
John P Tyminski and
Leslie D Zeigler
Abstract
The feeding anatomy, behavior and diet of the whale shark Rhincodon typus were studied off Cabo Catoche, Yucatan Peninsula, Mexico. The filtering apparatus is composed of 20 unique filtering pads that completely occlude the pharyngeal cavity. A reticulated mesh lies on the proximal surface of the pa...
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PMID: 20817493
PDF is available here.
Abstract
Diatoms are abundant in biofilms developed on surfaces immersed in sunlit waters. In both the planktonic and the biofilm mode of growth, diatoms produce carbohydrate polymers which perform several functions including motility, protection, production of macro-aggregates and detoxification. However, l...
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PMID: 20512708
PDF is available here.
Abstract
Despite the recent enthusiasm for using bacteriophages as bacterial control agents, there are only limited studies concerning phage interaction with their respective hosts residing in mixed biofilm consortia and especially in biofilms where the host species is a minor constituent. In the present wor...
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PMID: 20544433
PDF is available here.
Abstract
We studied the diversity and development of bacterioplankton communities from Prydz Bay (Eastern Antarctic) during spring and early summer 2002-2003. Additionally, we investigated the possible shaping effects of solar UV radiation (UV-R: 280-400 nm) on bacterioplankton communities incubated for 13-1...
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PMID: 20455939
PDF is available here.
Abstract
We observed that maximal allelopathic activity is highest in early growth stages of the cyanobacterium, and this provided sufficient material for isolation and chemical characterization of active compounds that inhibited the growth of C. vulgaris. Using a bioassay-guided approach, we isolated and st...
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PMID: 20534563
PDF is available here.
Abstract
We use recently developed time series analysis techniques drawn from population genetics to estimate the strength of net selection (time-averaged selection over a year) and fluctuating selection (an index of the variation in selection throughout the year) in natural plankton communities. Analysis of...
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PMID: 20583718
PDF is available here.
Abstract
We explored three different facets of the links that may exist between bacterioplankton compositional and functional successions that occurred along the water flow path in a complex watershed in southern Quebec. We analyzed the correlation between composition and function in terms of their absolute...
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PMID: 20503878
PDF is available here.
Abstract
The surface adherence and intracellular survival of E. faecalis within macrophages was significantly higher in biofilm bacteria when compared with planktonic cells (p
PDF is available here.
Abstract
Effects of cement flue dust from Ewekoro cement Kilns were monitored at some aquatic receptor locations. High levels of total suspended particulates (TSPs) and atmospheric deposition rates (ADRs) were recorded within the factory compared to ancillary locations outside the factory. The TSP and ADR le...
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PMID: 19247792
PDF is available here.
Abstract
We report several new examples of these giant bony fishes from Asia, Europe, and North America. These fossils provide the first detailed anatomical information on this poorly understood clade and extend its range from the lower Middle Jurassic to the end of the Cretaceous, showing that this group pe...
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PMID: 20167784
PDF is available here.
Abstract
These results also demonstrate the need for sufficient monitoring of biofilm recovery at the solid substratum if mechanical methods are used for biofouling control....
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PMID: 20023078
PDF is available here.
Abstract
This study presents a new technological approach to minimize the use of antimicrobial (AMB) agents and their deleterious effects, based on the principle of drug-delivery systems whereby the AMB chemicals are transported on microparticles. The efficacy of microparticles carrying the quaternary ammoni...
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PMID: 19937490
PDF is available here.
Abstract
We reported a noticeably seasonal, spatial (between lake branches), and vertical (between water column strata) heterogeneity in the total Hg concentrations found in the pelagic zone of Lake Moreno. Sterile water samples taken in a depth profile of the lake showed moderate to high concentrations of C...
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PMID: 21179954
PDF is available here.
Abstract
The aim of the present study was to evaluate the antimicrobial activity of new 2-(4-ethyl-phenoxymethyl) benzoic acid thioureides. The synthesis of the new compounds was done in three steps starting with the synthesis of 2-(4-ethyl-phenoxymethyl) benzoic acid. In the second stage, the 2-(4-ethyl-phe...
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PMID: 21235136
PDF is available here.
Abstract
We analyzed CYN concentration and polyketide synthase/peptide synthetase (PKS /PS) genes, important parts of the gene cluster responsible for the CYN biosynthesis, in 14 isolated/collected Cylindrospermopsis raciborskii and Aphanizomenon ovalisporum strains originated mostly from Hungary. CYN and PK...
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PMID: 21565779
PDF is available here.
Abstract
The results of an investigation carried out during October 2005 to September 2006 on hydrography composition and community structure of phytoplankton and zooplankton including chlorophyll 'a' (Chl-a) content and primary productivity (PP) at the Kaduviyar estuary (Southeast coast of India) are report...
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PMID: 20329402
PDF is available here.
Abstract
Acinetobacter baumannii is an important pathogen, capable of survival for very long periods on various surfaces in the hospital environment. Tigecycline is a commonly used antimicrobial agent especially for the treatment of resistant infections. The aim of this study was to investigate the activity...
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PMID: 20084911
PDF is available here.
Abstract
The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not...
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PMID: 19935890
PDF is available here.
Abstract
We report on the ability of surface-associated microbes to produce and release single planktonic cells to the bulk liquid as early as 6 h after attachment, with pure culture and mixed-species biofilms yielding up to approximately 1 x 10(6) cells/cm(2) of attachment area per hour to the effluent afte...
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PMID: 19935892
PDF is available here.
Abstract
The microsporidian Glugea pimephales was found parasitizing larval fathead minnow Pimephales promelas in Scott Lake, Algonquin Park, Ontario. These fish were estimated to be 2-3 weeks posthatch and, given the development time of the parasite, must have acquired infection soon after commencement of e...
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PMID: 20043401
PDF is available here.
Abstract
Pseudomonas aeruginosa, S. marcescens, and S. aureus reference and clinical strains formed biofilms on lotrafilcon A silicone hydrogel contact lenses, as dense networks of cells arranged in multiple layers with visible extracellular matrix. The biofilms were resistant to commonly used biguanide-pres...
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PMID: 19654521
PDF is available here.
Abstract
This study suggests that once the biofilm is formed, iron depletion may only slightly promote the bactericidal effect of antibiotics on PAO1, PAO-DeltapilHIJK and PAO-JP2. Although these changes were relatively small, iron as one of the environmental factors should not be ignored when evaluating bac...
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PMID: 19703377
PDF is available here.
Abstract
Trials were conducted aboard the tanker Seabulk Mariner to test a natural product, SeaKleen, as a biocide controlling non-indigenous populations of plankton and bacteria in ballast water. SeaKleen was dosed into matched ballast tanks at two different concentrations, 0.8 mg L(-1) active ingredient (a...
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PMID: 19803328
PDF is available here.
Abstract
Both ecological stoichiometry and the evolution of traits for energetic interactions such as prey protection and predatory efficiency are considered to be important aspects affecting population dynamics. However, no attempt has been made to examine the effect of the evolution of trai...
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PMID: 19298828
PDF is available here.
Abstract
Our results strongly support the idea of the flood pulse as the main factor driving the composition pattern of the planktonic ciliates community in the Paraná River floodplain....
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PMID: 19738959
PDF is available here.
Abstract
We show evidence of evolution within single-species biofilms in real time. Escherichia coli harvested from 22-day-old biofilms express a competitive advantage over cells incubated in biofilms for shorter periods of time. This advantage is manifested as the ability of aged cells to outcompete younger...
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PMID: 19239496
PDF is available here.
Abstract
Legionella pneumophila is a waterborne pathogen that has been isolated sporadically from drinking water distribution systems (DWDS). Resistance to disinfectants is mainly attributed to the association of cells with amoebae, but biofilms are also thought to provide some degree of protection. In...
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PMID: 19241230
PDF is available here.
Abstract
Animals with a gut, when confronted with food supplied ad libitum, can elevate their ingestion rates and inefficiently use the material they ingest. As a consequence, assimilation efficiency (AE) declines, resulting in food-density-dependent inefficiency (f-DDI). A model describing these processes s...
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PMID: 19129108
PDF is available here.
Abstract
We used background morphometric, hydrologic, physico-chemical and biological data, in order to assess the typology of the main Greek lakes. Correlation analysis and multivariate analysis (Principal Component Analysis) were applied for the data statistical elaboration. Lakes were classified according...
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PMID: 18509738
PDF is available here.
Abstract
This paper compares the fate and effects of linuron in an outdoor plankton-dominated microcosm study carried out in Thailand with those reported in temperate model ecosystem studies evaluating linuron and other photosynthesis-inhibiting herbicides. Lower linuron concentrations disappeared slightly f...
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PMID: 18722013
PDF is available here.
Abstract
We examined dynamics of dissolved DNA (D-DNA) and viruses along a transect in the phosphorus (P)-limited Ore Estuary, northern Baltic Sea. We found that viruses were an important mortality factor for bacterioplankton and that their activity mediated a significant recycling of carbon and especially o...
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PMID: 18670729
PDF is available here.
Abstract
We tracked the geochemistry and microbial community in Silver Lake playa, California, over one flooding/desiccation cycle following the unusually wet winter of 2004-2005. Over the course of the study, total dissolved solids increased by approximately 10-fold and pH increased by nearly one unit. As t...
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PMID: 18758846
PDF is available here.
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen of immunocompromised hosts. This pathogen has a tendency to form biofilms on the surface of indwelling catheters leading to acute and chronic urinary tract infections that result in significant morbidity and mortality. In the presen...
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PMID: 20701873
PDF is available here.
Abstract
This method is used to extract high molecular weight genomic DNA from planktonic biomass concentrated on 0.22 microM Sterivex filters that have been treated with storage/lysis buffer and archived at -80 degrees C, and to purify this DNA using a cesium chloride density gradient. The protocol begins w...
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PMID: 19767717
PDF is available here.
Abstract
Effects of chronic application of a mixture of the herbicide atrazine and the insecticide lindane were studied in indoor freshwater plankton-dominated microcosms. The macroinvertebrate community was seriously affected at all but the lowest treatment levels, the zooplankton community at the three high...
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PMID: 18757125
PDF is available here.
Abstract
I propose to establish the candidate species 'Candidatus Planktoluna difficilis', 'Candidatus Aquiluna rubra', 'Candidatus Flaviluna lacus', 'Candidatus Rhodoluna limnophila', 'Candidatus Rhodoluna planktonica', 'Candidatus Rhodoluna lacicola' and 'Candidatus Limnoluna rubra' for these taxa....
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PMID: 19126733
PDF is available here.
Abstract
Current classification of principal ecological groups of hydrobionts is an eclectic one as it confuses two fundamentals, one dealing with organismic ecomorphology and another with water body's topology. This leads to difficulties in determination of interrelations between benthos and fouling communi...
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PMID: 20063771
PDF is available here.
Abstract
Photodynamic disinfection (PDD) is a nonantibiotic approach to treating drug-resistant bacterial infections. Pseudomonas aeruginosa, an opportunistic pathogen, is problematic because of its propensity to develop antibiotic resistance and its ability to secrete a protective biofilm matrix. This study...
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PMID: 18673325
PDF is available here.
Abstract
We examined ultraviolet radiation (UVR)-induced DNA damage in marine micro-organisms collected from surface seawater along a latitudinal transect in the Central Pacific Ocean from 70 degrees N to 68 degrees S. Samples were collected predawn and incubated under ambient UVR in transparent incubators a...
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PMID: 19161407
PDF is available here.
Abstract
We observed that lysogenic infection of Pseudomonas aeruginosa PA14 with bacteriophage DMS3 inhibits biofilm formation and swarming motility, both important bacterial group behaviors. This inhibition requires the CRISPR region in the host. Mutation or deletion of five of the six cas genes and one of...
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PMID: 18952788
PDF is available here.
Abstract
Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in Bordetella pertussis, the agent responsible for whooping cough. Recent reports indicate that B. pertussis adopts a sessile biofilm as a strategy to persistently colonize t...
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PMID: 18972542
PDF is available here.
Abstract
These results are in agreement with those from semifield experiments with chlorpyrifos in temperate regions. The results of an in situ bioassay were used to calculate a NOEC of 0.1 microg/L and a 48-h median lethal concentration of 0.6 microg/L for M. micrura, which are similar to toxicity values re...
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PMID: 18699699
PDF is available here.
Abstract
Clear Lake is the site of the abandoned Sulphur Bank Mercury Mine, active periodically from 1873 to 1957, resulting in approximately 100 Mg of mercury (Hg) being deposited into the lake's ecosystem. Concentrations of total (primarily inorganic) Hg (TotHg) in Clear Lake are some of the highest report...
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PMID: 19475921
PDF is available here.
Abstract
Considerable ecological research on mercury (Hg) has focused on higher trophic level species (e.g., fishes and birds), but less on lower trophic species. Clear Lake, site of the abandoned Sulphur Bank Mercury Mine, provides a unique opportunity to study a system influenced by mine-derived Hg. An exp...
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PMID: 19475923
PDF is available here.
Abstract
These results indicate that even transient perturbations of the structure of freshwater food webs can result in significant alterations in the bioaccumulation of Hg and that food webs in lakes can be highly resilient....
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PMID: 19475926
PDF is available here.
Abstract
We describe the genome of one strain of the 'deep ecotype' (AltDE) isolated from 1000 m in the Eastern Mediterranean and compare this genome with that of the type strain ATCC 27126, a representative of the global 'surface' ecotype. The genomes are substantially different with DNA sequence similarity...
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PMID: 18670397
PDF is available here.
Abstract
We present a large-scale Southern Ocean observational analysis that examines the seasonal magnitude and variability of CO(3)(2-) and pH. Our analysis shows an intense wintertime minimum in CO(3)(2-) south of the Antarctic Polar Front and when combined with anthropogenic CO(2) uptake is likely to ind...
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PMID: 19022908
PDF is available here.
Abstract
We show that FCE is constrained by algal food quality and food-chain length. FCE across 3 trophic levels (phytoplankton to carnivorous fish) was highest under low light and high nutrients, where algal quality was best as indicated by taxonomic composition and nutrient stoichiometry. In 3-level syste...
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PMID: 19011082
PDF is available here.