Abstract
We hypothesized that these may be putative targets for C. collinus action and we tested this by exposing rat renal brush border membrane (BBM) as well as cultured kidney cells to a boiled decoction of C. collinus. Exposure to the C. collinus decoction resulted in significant inhibition of vacuolar t...
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PMID: 21824057
PDF is available here.
Abstract
Dipeptidyl peptidase IV is an ectopeptidase with multiple physiological roles including the degradation of incretins, and a target of therapies for type 2 diabetes mellitus. Divalent cations can inhibit its activity, but there has been little effort to understand how they act. The in...
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PMID: 21093607
PDF is available here.
Abstract
We synthesized the novel radioligand [(125)I]4-iodophenyl 1,4-diazaicyclo[3.2.2]nonane-4-carboxylate ([(125)I]CHIBA-1006), a iodine-derivative of the selective α7 nAChR agonist SSR180711, and studied the characterization of [(125)I]CHIBA-1006 binding to rat brain membranes. The assays of [(125)I]CH...
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PMID: 20816767
PDF is available here.
Abstract
I keratoprosthesis patients at 1 site who received Nd:YAG treatment for RPM from 2005 to 2009. Outcomes were based on slit-lamp examination of the optic immediately after YAG treatment, tactile intraocular pressure at follow-up, and complications from the outpatient procedure.
The incidence of RPM w...
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PMID: 20700050
PDF is available here.
Abstract
We have specifically investigated the correlation between neovascularization of membrane and the rate of hematoma absorption. Using improved surgical techniques, we have reduced surgery-related injury and increased the rate of generating successful SDH to 71.4%. With this model, we demonstrate that...
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PMID: 20486809
PDF is available here.
Abstract
A new solid support membrane aerated biofilm reactor was designed for the synthesis of enantiopure (S)-styrene oxide utilizing Pseudomonas sp. strain VLB120DeltaC growing in a biofilm as biocatalyst. In analogy to traditional packed bed systems, maximizing the volumetric oxygen mass...
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PMID: 20229513
PDF is available here.
Abstract
We hypothesized that U73122 acts as an inhibitor of cardiac PLD activity by interference with PIP2. U73122 concentration-dependently inhibited PLD activity in rat myocardial membranes. The inhibitory effect of U73122 was significantly attenuated when assayed on solubilized PLD activity and was compl...
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PMID: 20179606
PDF is available here.
Abstract
We tested the ability of THCV to activate CB(2) receptors by determining whether: (i) it inhibited forskolin-stimulated cyclic AMP production by Chinese hamster ovary (CHO) cells transfected with human CB(2) (hCB(2)) receptors; (ii) it stimulated [(35)S]GTPgammaS binding to hCB(2) CHO cell and mouse...
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PMID: 20590571
PDF is available here.
Tao Zhao,
Hiroshi Harada,
Yuji Teramura,
Shotaro Tanaka,
Satoshi Itasaka,
Akiyo Morinibu,
Kazumi Shinomiya,
Yuxi Zhu,
Hirofumi Hanaoka,
Hiroo Iwata,
Hideo Saji and
Masahiro Hiraoka
Abstract
We established a novel method of specifically tagging the surface of MMP2- and MT1-MMP-positive cells, and applied it to the development of an optical imaging probe. We constructed a protein-based probe composed of a glutathione-S-transferase (GST)-tag (Inhibitory [I]-domain), a polypeptide as a spe...
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PMID: 20096316
PDF is available here.
Abstract
The Keystone Symposium on the Molecular Basis for Biological Membrane Organization and Dynamics held in January this year offered new insights into the molecular machines at work in cells. Topics included the machinery responsible for the dynamic shape of organelles, the budding and fusion of vesicu...
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PMID: 20395954
PDF is available here.
Abstract
The recent development of various membrane-based techniques for the purification of valuable natural products is reviewed and covers the important research that has been conducted in the last 5 years on the utilization of microfiltration, ultrafiltration and nanofiltration techniques, either carried...
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PMID: 20049625
PDF is available here.
Abstract
We present a technique for repairing a perforated Schnei-derian membrane with a de-epithelialized fibromucosal graft harvested from the palate of a 50-year-old man. In the postoperative period, no wound infections, sinusitis, or bleeding were observed. This technique allowed good prosthetic rehabili...
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PMID: 20502434
PDF is available here.
Abstract
This study formulates governing equations for active transport across semi-permeable membranes within the framework of the theory of mixtures. In mixture theory, which models the interactions of any number of fluid and solid constituents, a supply term appears in the conservation of linear momentum...
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PMID: 20213212
PDF is available here.
Abstract
We have found that on the ventral side most of these patches have very transparent scales that enhance, by reflection, the wing coloration when illuminated from the dorsal side. These glass scales furthermore create a strongly polarized iridescence when illuminated by obliquely incident light from t...
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PMID: 20435824
PDF is available here.
Abstract
We trained a learning algorithm on several water-soluble and insoluble helical peptides by comparing its predictions with experimental solubility and fluorescence vesicle binding assays. The algorithm yielded an easily computed score S to discover soluble peptides in databases of transmembrane helic...
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PMID: 20232924
PDF is available here.
Abstract
Ionic liquids (ILs) have been applied in different areas of separation, such as ionic liquid supported membranes, as mobile phase additives and surface-bonded stationary phases in chromatography separations and as the extraction solvent in sample preparations, because they can be composed from vario...
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PMID: 20428052
PDF is available here.
Abstract
My research has included chemical physics, electron and NMR spectroscopy, membrane biophysics, and immunology. This research was curiosity driven as well as problem and technique oriented. A theoretical equation was developed for relating nuclear hyperfine splittings to electron spin distributions i...
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PMID: 20462374
PDF is available here.
Abstract
The PRF protocol concentrated most platelets and leukocytes from a blood harvest into a single autologous fibrin biomaterial. This protocol offers reproducible results as long as the main production principles are respected....
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PMID: 20373539
PDF is available here.
Abstract
The eggshell membrane (ESM) contains several surface functional groups such as amines, amides and carboxylic groups with potential as SPE adsorbent for the retention of target species of interest. In this paper, the potential use of ESM, a typical biomaterial, as solid-phase extraction (SPE) adsorbe...
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PMID: 20152431
PDF is available here.
Abstract
We have shown that sperm integrin alpha6beta1 plays a determinant role in mouse gamete interaction. In this study, we demonstrate the presence of alphavbeta3 integrin by Western blot and immunofluorescence on the sperm membrane. Oocytes and/or sperm preincubations with anti-alphav or anti-beta3 anti...
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PMID: 20151413
PDF is available here.
Abstract
Membrane technology is broadly applied in the medical field. The ability of membranous systems to effectively control the movement of chemical entities is pivotal to their significant potential for use in both drug delivery and surgical/medical applications. An alteration in the physical properties...
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PMID: 20300895
PDF is available here.
Abstract
The main characteristic of uridine diphosphate (UDP)-glucuronosyltransferases is their potency to glucuronidate a large array of structurally unrelated substances with various nucleophilic groups. The activity of these enzymes strongly depends on their tight association to the membrane of the endopl...
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PMID: 19807219
PDF is available here.
Abstract
We report the identification and characterization of galectin-3 in human semen. In the male reproductive tract, the approximately 30 kDa galectin-3 protein was identified in testis, epididymis, vas deferens, prostate, seminal vesicle, and sperm protein extracts. In seminal plasma, galectin-3 was ide...
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PMID: 19830550
PDF is available here.
Abstract
We present a detailed analysis of a Methanosarcina mazei Delta ech mutant which shows decreased ferredoxin-dependent membrane-bound electron transport activity, a lower growth rate, and faster substrate consumption. Evidence is presented that a second protein whose identity is unknown oxidizes reduc...
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PMID: 19948802
PDF is available here.
Abstract
I will first discuss some key notions, technical analogies, and images that are related to "fibre"-concepts from Andreas Vesalius to Albrecht von Haller and then focus on Charles Bonnet's and Denis Diderot's fibre ceconomies. In Bonnet's and Diderot's fibre economies, the self-active, regulating pro...
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PMID: 20499615
PDF is available here.
Abstract
Aberrantly folded proteins and peptides are hallmarks of amyloid diseases. A deeper knowledge of the pathways leading to the formation of amyloid protein aggregates and of the mechanisms of their cytotoxicity is fundamental for a better understanding of several human diseases with amyloid deposition...
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PMID: 20700716
PDF is available here.
Abstract
Occupational and environmental Pb-exposure is associated with protein aggregation diseases which typically present in elderly populations (Parkinsons and cataract). Post-translational processing of crystallins, the major structural proteins of the lens, is altered with short-term Pb-exposure in Fish...
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PMID: 21139692
PDF is available here.
Abstract
This study reports on an investigation into apoptotic and proliferation signals in leukocyte and membrane fibroblasts in periprosthetic membranes collected during revision surgery for loosened total hip joint arthroplasty. Cementless and cemented prosthesis were studied under both aseptic and septic...
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PMID: 19165796
PDF is available here.
Abstract
All biomolecular constructions and nanorecators are designed to perform preset functions. All of them operate with limited reliability, namely, for each and every device or bionanoreactor normal operation alternates with accidental malfunctions (failures). Timely preventive maintenance replacement (...
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PMID: 20734796
PDF is available here.
Abstract
TEM image processing tools are devised for the assessment of 2D-crystallization experiments. The algorithms search for the presence and assess the quality of crystalline membranes. The retained scenario emulates the decisions of a microscopist in selecting targets and assessing the sample. Crystalli...
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PMID: 20865516
PDF is available here.
Abstract
Histopathologically, basal membranes were thickened and there was intense inflammatory reaction in diabetic lungs. However, the PDTC group, in which there were poor positive expressions of eNOS and p65 activity compared to diabetes group, revealed fewer inflammatory changes. PCC levels in diabetic l...
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PMID: 19996572
PDF is available here.
Abstract
Human adenoviruses (HAdV) and hepatitis A virus (HAV) are shed in the faeces and consequently may be present in environmental waters, resulting in an increase in pathogen concentration that can affect water quality and human health. The aim of this study was to evaluate an adsorption-elution method...
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PMID: 20027462
PDF is available here.
Abstract
Proliferative vitreoretinopathy (PVR) appears to be one of the main reasons of functional and sometimes also anatomical death of the eye after its damages and diseases as well as is one of the heavy complications of the post operation period at vitreoretinal surgery. PVR is characterized by the occu...
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PMID: 19893117
PDF is available here.
Abstract
Activities of carboxylesterase and malate dehydrogenase on membranes were retained after enzymes of mouse liver cytosol were separated by non-denaturing, two-dimensional electrophoresis (2-DE), stained using imidazole and zinc salts and electroblotted on to membranes. Furthermore, hydrolytic changes...
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PMID: 19517067
PDF is available here.
Abstract
The pH-triggered membrane insertion pathway of the T-domain of diphtheria toxin was studied using site-selective fluorescence labeling with subsequent application of several spectroscopic techniques (e.g., fluorescence correlation spectroscopy, FRET, lifetime quenching, and kinetic fluorescence). FC...
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PMID: 19588969
PDF is available here.
Abstract
Chloroperoxidase (CPO) from Caldariomyces fumago was optimally covalently immobilized on chitosan membranes pretreated with 0.8 M glutaraldehyde at pH 3.5 to give 3.18 mg CPO g(-1) support. Using monochlorodimedone (MCD) as assay substrate, the immobilized-CPO retained 40% activity at 50 degrees C a...
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PMID: 19404743
PDF is available here.
Abstract
We treated immortalized non-tumor breast cells with 5 or 50 microM CdCl(2) for 24 or 96 h demonstrating that the exposure did not cause a significant mitochondrial proliferation, while it induced a significant increase in the respiratory activity and mitochondrial polarization. In addition, we found...
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PMID: 19266167
PDF is available here.
Abstract
Cyclic voltammetry is used to investigate the transfer of alkali-metal cations, protons, and ammonium ions facilitated by the complex formation with valinomycin at the interface between an aqueous electrolyte solution and a room-temperature ionic liquid (RTIL) membrane. The membrane is made of a thi...
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PMID: 19572695
PDF is available here.
Abstract
We demonstrate the application of the technique to liposomes reconstituted with the peptide cation channel gramicidin, vesicles from native tissue containing the nicotinic acetylcholine receptor, and membranes from a recombinant cell line expressing the ionotropic P2X2 receptor. It is shown that sta...
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PMID: 19580777
PDF is available here.
Abstract
Our results support the general perception that the biomembrane is crucial for correct positioning and embedding of its constituents. The described model provides a semi-quantitative tool of potential interest to many problems in structural membrane biology....
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PMID: 19416726
PDF is available here.
Abstract
despite the fact that there are only sporadic reports of cases of membranous dysmenorrhea in the scientific literature, this etiology must be considered in cases of pain associated with vaginal bleeding plus elimination of elastic or solid material. The final diagnosis depends on anatomopathological...
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PMID: 19684966
PDF is available here.
Abstract
Subretinal membranes in AMD patients consisted mainly of fibroblasts, isolated or grouped RPE cells and elements of blood. Numerous capillaries of subretinal blood vessels were also found. Their walls consisted only of one thin layer of fenestrated endothelial cells. The endothelial fenestration in...
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PMID: 19507100
PDF is available here.
Abstract
Ophthalmoscopy showed a retinal detachment with a giant tear in the right eye, and a nonpigmented epithelial detachment with pars plicata breaks in the left eye. Bilateral findings included an empty vitreous and a vitreous membrane at the equator. The systemic abnormalities included short stature an...
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PMID: 19089441
PDF is available here.
Abstract
Mixed matrix membranes (MMMs), which incorporate adsorptive particles during membrane casting, can be prepared simply and have performances that are competitive with other membrane chromatography materials. The application of MMM chromatography for fractionation of beta-Lactoglobulin from bovine whe...
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PMID: 19199353
PDF is available here.
Abstract
We examined the role of (1) extracellular Ca(2+); (2) voltage-induced influx of external Ca(2+) ions; (3) sarcoplasmic reticulum (SR) Ca(2+) depletion during repeated contractions; (4) store-operated Ca(2+) entry (SOCE); (5) SR ultrastructure; (6) SR subdomain localization of the ryanodine receptor;...
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PMID: 18948183
PDF is available here.
Abstract
A theoretical model for filtration of large solutes through a pore in the presence of transmembrane pressures, applied/induced electric fields, and dissimilar interactions at the pore entrance and exit is developed to characterize and predict the experimental performance of a hemofiltration membrane...
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PMID: 19184436
PDF is available here.
Abstract
We discover a rich phase diagram. In particular we show that the density of motors along the tube can exhibit shocks, inverse shocks, and plateaux, depending on parameters which could in principle be probed experimentally. In addition the phase diagram exhibits interesting reentrant behavior....
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PMID: 19392247
PDF is available here.
Abstract
The influence of a variety of microenvironmental factors on the inherent reactivity of membrane-located reagents is poorly understood. A goal of this review is to provide detailed profiles of membrane properties, including hydrophobicity, oxygen and nitric oxide solubility and diffusion rates, bilay...
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PMID: 19111611
PDF is available here.
Abstract
After 3 weeks of implantation, the wound surface of control group were observed nigrescene, scab and putrescence, and after 8 weeks of implantation, also evident putrescence and scar. The wound surface of experiment group was alive after 3 weeks implantation, appearance was leveled off and flexible...
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PMID: 19366152
PDF is available here.
Abstract
Egg shell membrane is a novel, robust, microporous, cost effective, easily available organic support material. In recent studies egg shell membranes were utilized as organic support for enzyme immobilization. But low conjugation yield limits its application as good support for biotechnological indus...
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PMID: 19159784
PDF is available here.
Abstract
In all specimens, the pituitary glands were found to be coated by 2 membranous layers, the inner layer being referred to here as the lamina propria and the outer as the pituitary capsule. In all specimens, the 2 membranes were intact with no histological defects. An interstice or cavity between the...
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PMID: 19240557
PDF is available here.
Abstract
Chemical penetration enhancers are widely used in transdermal pharmaceuticals as well as cosmetic products. Selection of suitable enhancers in topical formulations requires an understanding of the mechanism of action of these enhancers. The objective of the present study was to evaluate the enhancem...
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PMID: 18623209
PDF is available here.
Abstract
We evaluate methodologies and null mouse models used to study drug transporter function
in vitro and
in vivo. P-glycoprotein and
MRP null mice have been used to examine many aspects of xenobiotic distribution and bioavailability. Their advantage over conventional models is that...
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PMID: 19514970
PDF is available here.
Abstract
Eleven of 16 (69%) choroidal neovascular membranes stained positive for COX-2 in retinal pigment epithelial cells, with 6 (38%) of these also expressing COX-2 in vascular endothelial cells and 6 (38%) in fibroblasts. None of the sections that were negative for COX-2 in the retinal pigment epithelial...
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PMID: 18827739
PDF is available here.
Abstract
We study a thermodynamic model for the stability of a protein-decorated binary lipid layer. Here, proteins interact preferentially with one lipid species and thus locally sequester that species. We aim to specify conditions that lead to an additional macroscopic phase separation of the protein-decor...
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PMID: 19191415
PDF is available here.
Abstract
We evaluated a novel purification process combining an anion-exchange chromatography and an immobilized metal affinity membrane chromatography for the purification of recombinant adenovirus. Adenovirus was initially purified from clarified infectious lysate by anion-exchange chromatography using Q S...
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PMID: 19056351
PDF is available here.