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Virion (1):
Recent article
Virion
Nanomedicine (6)2 2011
Abstract
We investigated the expression of surface vimentin on cancer cells and evaluated targeting nanoparticles to tumors exploiting vimentin.
Cowpea mosaic virus nanoparticles that interact with surface vimentin were used as probes. Tumor homing was tested using the chick chorioallantoic m...
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PMID: 21385137
PDF is available here.
PDF is available here.
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Abstract
We compared enfuvirtide susceptibility and infectivity of recombinant viral pairs harboring the HR1-HR2 region or the full Env ectodomain of longitudinal env clones from 5 heavily treated patients failing enfuvirtide therapy. Prior to enfuvirtide treatment onset, no env carried known resistance muta...
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PMID: 21760896
PDF is available here.
PDF is available here.
Abstract
We demonstrated that the G1613A mutation in the HBV negative regulatory element (NRE) is a hotspot mutation in HCC patients. In this study, we further investigated the functional consequences of this mutation in the context of the full length HBV genome and its replication. We showed that the G1613A...
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PMID: 21814558
PDF is available here.
PDF is available here.
Abstract
We have previously shown that the hepatic toxicity caused by wild-type Adenovirus 5 (Ad5WT) in mice can be prevented by incorporating 4 binding sites for the liver-specific microRNA, mir122, into the 3' UTR of E1A mRNA. This virus, termed Ad5mir122, is a promising virotherapy candidate and causes no...
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PMID: 21264344
PDF is available here.
PDF is available here.
Abstract
Previously, a modified HIV Env protein with a heterologous membrane anchor was found to be incorporated into HIV virus-like particles (VLPs) at 10-fold-higher levels than those of unmodified Env. To further improve the immunogenicity of such VLPs, membrane-anchored forms of bacterial...
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PMID: 21325038
PDF is available here.
PDF is available here.
Abstract
The human pathogen, group A rotavirus, is the most prevalent cause of acute infantile and pediatric gastroenteritis worldwide, especially in developing countries. There is an urgent demand for safer, more effective and cheaper vaccines against rotavirus infection. Plant-derived antig...
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PMID: 21104033
PDF is available here.
PDF is available here.
Retrovirology (8)1 2011
Abstract
We investigated the importance of the highly conserved serine residue at position 40, which until now has not been assigned to any known function of p6.
Consistently with previous data, we found that mutation of Ser-40 has no effect on ALIX mediated rescue of HIV-1 L-domain mutants....
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PMID: 21324168
PDF is available here.
PDF is available here.
Abstract
Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells can self-renew while maintaining their pluripotency to differentiate into virtually all cell types. In addition to their potential for regenerative medicine, hESCs and iPSCs can also serve a...
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PMID: 21042988
PDF is available here.
PDF is available here.
Abstract
We report the crystal structures of the precursor p62-E1 heterodimer and of the mature E3-E2-E1 glycoprotein complexes. The resulting atomic models allow the synthesis of a wealth of genetic, biochemical, immunological and electron microscopy data accumulated over the years on alphaviruses in genera...
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PMID: 21124458
PDF is available here.
PDF is available here.
Abstract
We report the structure of an alphavirus spike, crystallized at low pH, representing an intermediate in the fusion process and clarifying the maturation process. The trimer of E2-E1 in the crystal structure is similar to the spikes in the neutral pH virus except that the E2 middle region is disorder...
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PMID: 21124457
PDF is available here.
PDF is available here.
Abstract
Virus/Uirusu (60)2 2010
Abstract
Herpes simplex virus (HSV), the prototype of the herpesvirus family, causes a variety of diseases in human. In this review, I focus on the molecular mechanism of HSV infection including recent advance on this research field.
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PMID: 21488332
PDF is available here.
PDF is available here.
Virus/Uirusu (60)2 2010
Abstract
Human cytomegalovirus (HCMV) is a ubiquitous beta human herpesvirus type 5. Compared to other human herpesviruses, HCMV is the largest, with a genome of approximately 235 kb containing approximately 250 ORFs with the potential to encode proteins. Usually, HCMV asymptomatically infects the host during...
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PMID: 21488334
PDF is available here.
PDF is available here.
Abstract
These results illustrate the great progress that has been made in the HCV field and how this knowledge can be used to devise innovative strategies to counteract this pathogen.
© Thieme Medical Publishers....
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PMID: 20960374
PDF is available here.
PDF is available here.
Abstract
We found that the ORF0 of both strains was incorporated into virus particles, the C-terminal region of vOka ORF0 was presented on the virion surface and was N-glycosylated, suggesting that the mutation in vOka ORF0 changes it into a novel envelope glycoprotein. In a mutant virus in which pOka ORF0 w...
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PMID: 20598727
PDF is available here.
PDF is available here.
Abstract
We exchanged the HPV18 L2 or L1 genes with those of HPV16. The intertypical exchange of HPV18 L1 with the HPV16 L1 produced genomes that efficiently replicated and produced infectious virus. Genomes containing an intertypical exchange of HPV18 L2 for the HPV16 L2 failed to produce infectious virus i...
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PMID: 20598725
PDF is available here.
PDF is available here.
Mucosal Immunology (3)5 2010
Abstract
We produced mucosal vaccine vectors by HPV PsV encapsidation of DNA plasmids expressing an experimental antigen derived from the M and M2 proteins of respiratory syncytial virus (RSV). Intravaginal (IVag) delivery elicited local and systemic M-M2-specific CD8+ T-cell and antibody responses in mice t...
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PMID: 20555315
PDF is available here.
PDF is available here.
Abstract
We discuss the influenza A virion structure and its replication steps in host cells....
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PMID: 20845736
PDF is available here.
PDF is available here.
Biophysical Journal (99)4 2010
Abstract
We studied the control parameters that govern the dynamics of in vitro DNA ejection in bacteriophage lambda. Previous work demonstrated that bacteriophage DNA is highly pressurized, and this pressure has been hypothesized to help drive DNA ejection. Ions influence this process by screening charges o...
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PMID: 20712993
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular pa...
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PMID: 20519400
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We performed random mutagenesis of PrV pUL36 by transposon-mediated insertion of a 15-bp linker. By this approach, 26 pUL36 insertion mutants were selected and tested in transient transfection assays for their ability to complement one-step growth and/or viral spread of a PrV UL36 null mutant. Ten i...
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PMID: 20534865
PDF is available here.
PDF is available here.
Journal of General Virology (91)Pt 8 2010
Abstract
Cell entry and membrane fusion of the hepatitis C virus (HCV) depend on its envelope glycoproteins E1 and E2. HCV pseudotyped particles (HCVpps) are relevant and popular models to study the early steps of the HCV life cycle. However, no structural characterization of HCVpp has been available so far....
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PMID: 20375221
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)30 2010
Abstract
We found that the 5' termini of the transcripts are diphosphorylated. The 5'-terminal nucleotide is G, and GDP was the best substrate among those examined to prime the reaction. When GTP was used, the triphosphate of GTP incorporated into the 5'-end was converted to diphosphate. This activity was no...
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PMID: 20511225
PDF is available here.
PDF is available here.
Abstract
We developed a direct xenograft model, UM-PEL-1, by transferring freshly isolated human PEL cells into the peritoneal cavities of NOD/SCID mice without in vitro cell growth to avoid the changes in KSHV gene expression evident in cultured cells. We used this model to show that bortezomib induces PEL...
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PMID: 20615981
PDF is available here.
PDF is available here.
Gene Therapy (17)7 2010
Abstract
We determined the infectious, physical and genome-containing particle titers of several wild-type AAV type 2 (wtAAV2) and recombinant AAV type 2 (rAAV2) preparations that were produced and purified by standard methods. We found that the infectivity of wtAAV2 approaches a physical-to-infectious parti...
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PMID: 20336156
PDF is available here.
PDF is available here.
Abstract
We investigated the post-binding mechanism of recombinant core particle uptake and its intracellular fate. In hepatic cells, these particles are internalized, most likely in a clathrin-dependent pathway, reaching early to late endosomes and finally lysosomes. The endocytic acidic milieu is implicate...
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PMID: 20358251
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We have used cryoelectron tomography to investigate the morphotypes of this double mutant. Virions produced at the nonpermissive temperature do not assemble capsids, although Gag is processed normally; moreover, they are more variable in size than the wild type and have fewer glycoprotein spikes. At...
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PMID: 20427531
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We report here the results of a structure/function analysis in which the role of conserved cysteine residues, clusters of charged amino acids and clusters of hydrophobic/aromatic amino acids have been assessed. Taking advantage of a recombinant virus in which F18 expression is IPTG (isopropyl-beta-d...
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PMID: 20392848
PDF is available here.
PDF is available here.
Cancer Research (70)12 2010
Abstract
We show that compared with nontransformed cells, Ras-transformed cells are severely compromised not only in their response to IFN-beta, but also in the induction of IFN-beta mRNA following reovirus infection. Defects in both IFN-beta production and response allow for efficient virus spread in Ras-tr...
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PMID: 20501842
PDF is available here.
PDF is available here.
Biochemistry (Washington) (49)23 2010
Abstract
We monitored structural changes occurring in HCV core protein and loop IIId upon formation of nucleocapsid-like particles (NLPs). The protein secondary structure of these particles involves beta-sheet enrichment in relation to its protein monomer. The phosphodiester backbone vibrations of loop IIId...
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PMID: 20469947
PDF is available here.
PDF is available here.
Abstract
We combine electron cryotomography and analysis of images of frozen-hydrated virions to determine the structural organization of filamentous influenza A virus. Influenza A/Udorn/72 virions are capsule-shaped or filamentous particles of highly uniform diameter. We show that the matrix layer adjacent...
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PMID: 20498070
PDF is available here.
PDF is available here.
Abstract
Genetically engineered retroviruses are widely used for gene delivery into human cells. A number of investigators have studied spleen necrosis virus (SNV) as a vehicle for gene delivery. Vectors developed from SNV and its closely associated avian reticuloendotheliosis virus strain A (REV-A) can be u...
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PMID: 20516173
PDF is available here.
PDF is available here.
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (18)6 2010
Abstract
We constructed a stable HCV-producing cell model which can be used for anti-HCV drug research....
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PMID: 20587314
PDF is available here.
PDF is available here.
Archives of Virology (155)6 2010
Abstract
Tomatoes in Guatemala have been affected by a new disease, locally known as "mancha de chocolate" (chocolate spot). The disease is characterized by distinct necrotic spots on leaves, stems and petioles that eventually expand and cause a dieback of apical tissues. Samples from symptomatic plants test...
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PMID: 20376682
PDF is available here.
PDF is available here.
Archives of Virology (155)6 2010
Abstract
We demonstrate that in non-permissive cells, the replicative cycle is more advanced in FP than in CP, that human cells, whether immune or not, are less permissive to avipox replication than monkey cells, and that the presence of virus-like particles only occurs after FP infection. Overall, these dat...
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PMID: 20379750
PDF is available here.
PDF is available here.
Journal of Virological Methods (166)1-2 2010
Abstract
The immunogenic properties of chimeric potato virus X (PVX) particles engineered to display the synthetic R9 peptide have been evaluated. The R9 peptide is a consensus sequence derived from diverse variants of the hypervariable region 1 from the hepatitis C virus (HCV) envelope protein E2. Two diffe...
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PMID: 20138085
PDF is available here.
PDF is available here.
Virus/Uirusu (60)1 2010
Abstract
We have been focusing on Sendai virus (SeV) and shown that (i) SeV M functionally and physically interact with a component of the ESCRT complex, Alix/AIP1, although budding of M-VLPs does not seem to be dependent on the pathway; (ii) one of the accessory proteins of SeV, C, also interact with Alix/A...
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PMID: 20848870
PDF is available here.
PDF is available here.
Virus/Uirusu (60)1 2010
Abstract
Hepatitis C virus (HCV) establishes a persistent infection and is recognized as a major cause of chronic liver diseases worldwide. Although much work remains to be done regarding the viral life cycle, significant progress has been made with respect to the molecular biology of HCV, especially the vir...
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PMID: 20848868
PDF is available here.
PDF is available here.
Abstract
We have developed a complementary approach in which viral and synthetic components are combined to form hybrid nanoparticulate vectors. In particular, we complexed non-infectious retrovirus-like particles lacking a viral envelope protein, from Moloney murine leukemia virus (M-VLP) or human immunodef...
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PMID: 20117154
PDF is available here.
PDF is available here.
Abstract
We designed a chimeric J6/JFH-1 virus with a FLAG (FL)-epitope-tagged sequence at the N-terminal region of the E2 hypervariable region-1 (HVR1) gene (J6/JFH-1/1FL). We found that introduction of an adaptive mutation at the potential N-glycosylation site (E2N151K) leads to efficient production of the...
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PMID: 20399750
PDF is available here.
PDF is available here.
Abstract
Abstract
We identified protein binding partners for two viral proteins, pUL99 (also termed pp28) and pUL32 (pp150), which are essential for HCMV virion assembly. We utilized HCMV strains expressing pUL99 or pUL32 carboxyl-terminal green fluorescent protein fusion proteins from their native location in the HC...
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PMID: 20023299
PDF is available here.
PDF is available here.
Abstract
Pseudomonas chlororaphis phage 201 phi 2-1 produces a large structurally complex virion, including the products of 89 phage genes. Many of these proteins are modified by proteolysis during virion maturation. To delineate the proteolytic maturation process, 46 slices from an SDS-polyacrylamide gel we...
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PMID: 20233846
PDF is available here.
PDF is available here.
Human Gene Therapy (21)5 2010
Abstract
We therefore developed a direct integrase fusion protein strategy as an alternative way to package heterologous proteins into vectors. The method was tested by creating two different integrase fusion proteins, IN-p53 and IN-mCherry, cloned into the 3' end of pol in the packaging plasmid. Lentiviral...
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PMID: 20039782
PDF is available here.
PDF is available here.
Abstract
Diagnosis of HBoV primary infection can be strengthened by measurement of IgG avidity. HBoV secondary infections or anamnestic antibody responses occur ubiquitously in immunocompetent adults.
Copyright 2010 Elsevier B.V. All rights reserved....
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PMID: 20227338
PDF is available here.
PDF is available here.
Abstract
The Cucumber mosaic virus (CMV) is an isodiametric plant virus with an extremely wide host range, present worldwide. CMV chimeric particles (R9-CMV), engineered to express a 27-aa synthetic peptide derived from Hepatitis C virus (HCV), were demonstrated to be stable under simulated gastric and intes...
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PMID: 20132840
PDF is available here.
PDF is available here.
Abstract
Our findings could have significance for human diseases such as viral encephalitis and multiple sclerosis....
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PMID: 20038206
PDF is available here.
PDF is available here.
Virus Genes (40)2 2010
Abstract
We asked whether the C-terminal scaffolding domain of gpO could work as a delta domain when fused to the gpN capsid protein. Varying lengths of C-terminal sequences from gpO were fused to the N-terminus of gpN and expressed in E. coli. The presence of just the 41 C-terminal residues of gpO increased...
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PMID: 20063181
PDF is available here.
PDF is available here.
Abstract
We report a near-atomic-resolution reconstruction of the GCRV virion by cryo-electron microscopy and single-particle reconstruction. A backbone model of the GCRV virion, including seven conformers of the five capsid proteins making up the 1500 molecules in both the core and the coat, was derived usi...
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PMID: 20036256
PDF is available here.
PDF is available here.
Avian Pathology (39)2 2010
Abstract
Haemorrhagic nephritis enteritis of the goose (HNEG) is an epizootic viral disease in domestic geese. The causal agent is a polyomavirus, namely goose haemorrhagic polyomavirus. To help control the disease, an inactivated vaccine was developed, based on viral particles produced in goose kidney cells...
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PMID: 20390545
PDF is available here.
PDF is available here.
Methods in Molecular Biology (614)614 2010
Abstract
We describe a system relying on the finding that some HIV-1 Nef mutants are incorporated at high levels into both Human Immunodeficiency virus (HIV)-1 and Moloney Leukemia Virus (MLV)-based VLPs. Most importantly, these Nef mutants can efficiently act as anchoring proteins upon fusion with heterolog...
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PMID: 20225039
PDF is available here.
PDF is available here.
Abstract
We determined the impact of mucosal prime/boost regimens and vaccine type (attenuated Wa human rotavirus [AttHRV] or nonreplicating Wa 2/6 rotavirus-like particles [VLP]) on protection and antibody-secreting cell (ASC) responses to HRV in a neonatal gnotobiotic pig disease model. Comparisons of deli...
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PMID: 20107005
PDF is available here.
PDF is available here.
Abstract
A nasal adenovirus-based vaccine is under development. To determine if aggregation occurs during vaccination, infectious titer (limiting dilution) and capsid integrity (dynamic light scattering) were assessed after extrusion of a model vector from two intranasal delivery devices. Preparations of 2.5...
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PMID: 20044048
PDF is available here.
PDF is available here.
Abstract
We show that vaccinia virus spreads across one cell every 75 minutes, fourfold faster than its replication cycle would permit. To explain this phenomenon, we found that newly infected cells express two surface proteins that mark cells as infected and, via exploitation of cellular machinery, induce t...
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PMID: 20093437
PDF is available here.
PDF is available here.
Abstract
Abstract
We show that each virion contains two nested, left-handed helices: an outer helix of matrix protein M and an inner helix of nucleoprotein N and RNA. M has a hub domain with four contact sites that link to neighboring M and N subunits, providing rigidity by clamping adjacent turns of the nucleocapsid...
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PMID: 20133572
PDF is available here.
PDF is available here.
Determinants for the rhesus monkey TRIM5alpha-mediated block of the late phase of HIV-1 replication.
Abstract
We reported that TRIM5alpharh also blocks HIV-1 production in a B30.2(PRYSPRY)-independent manner. Efficient encapsidation of TRIM5alpharh, but not human TRIM5alpha (TRIM5alphahu), in HIV-1 virus-like particles suggests the interaction between Gag and TRIM5alpharh during viral assembly. Here, we det...
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PMID: 19951947
PDF is available here.
PDF is available here.
Abstract
We report the complete Euprosterna elaeasa virus (EeV) genome sequence of 5698 nt with no poly(A) tail and two overlapping open reading frames, encoding the replicase and capsid precursor, with approximately 67% amino acid identity to Thosea asigna virus (TaV). The N-terminally positioned 17 kDa pro...
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PMID: 19954807
PDF is available here.
PDF is available here.
Abstract
We investigated the physical relationship between TCS and HIV-1 particles, and demonstrated that TCS penetrates into viral particles, where it is protected from various protease digestion. The penetration of TCS exerts no obvious effect on viral integrity. FYY140-142, D176, and K177 were identified...
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PMID: 20119629
PDF is available here.
PDF is available here.
Abstract
The stabilities of the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) complete genome bacmid (Sfbac) and a deletion recombinant (Sf29null) in which the Sf29 gene was replaced by a kanamycin resistance cassette were determined during sequential rounds of per os infection in insect larva...
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PMID: 20008167
PDF is available here.
PDF is available here.